Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0838
(573 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa... 140 6e-34
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica... 137 6e-33
At4g35410.1 68417.m05029 clathrin adaptor complex small chain fa... 122 2e-28
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 103 8e-23
At1g47830.1 68414.m05324 clathrin coat assembly protein, putativ... 99 2e-21
At3g50860.1 68416.m05569 clathrin adaptor complex small chain fa... 79 2e-15
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 33 0.10
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun... 33 0.14
At4g24550.2 68417.m03519 clathrin adaptor complexes medium subun... 31 0.55
At4g24550.1 68417.m03518 clathrin adaptor complexes medium subun... 31 0.55
At2g13500.1 68415.m01489 hypothetical protein similar to zinc fi... 28 5.1
At5g11040.1 68418.m01290 expressed protein weak similarity to hy... 27 6.7
At1g56590.1 68414.m06508 clathrin adaptor complexes medium subun... 27 8.9
>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 162
Score = 140 bits (339), Expect = 6e-34
Identities = 61/97 (62%), Positives = 76/97 (78%)
Frame = +1
Query: 259 RELITTVLARKPKMCSFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLD 438
REL +L R PK+C+F+EW+ KVVYKRYASLYFC ++QEDNEL LE+IH YVE+LD
Sbjct: 33 RELSGVILNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEIIHHYVEILD 92
Query: 439 KYFGXVCELDIIFNFEKAYFILDDWFLGGXLQVNPVK 549
+YFG VCELD+IFNF KAY+ILD+ + G LQ + K
Sbjct: 93 RYFGSVCELDLIFNFHKAYYILDELLIAGELQESSKK 129
Score = 35.1 bits (77), Expect = 0.034
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +2
Query: 164 MMQFMLLFSRQGKLRLQKWY 223
M+ F+LL SRQGK+RL KWY
Sbjct: 1 MIHFVLLVSRQGKVRLTKWY 20
>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
to clathrin assembly protein AP19 GI:2231698 from
[Arabidopsis thaliana]
Length = 161
Score = 137 bits (331), Expect = 6e-33
Identities = 59/97 (60%), Positives = 75/97 (77%)
Frame = +1
Query: 259 RELITTVLARKPKMCSFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLD 438
REL +L R PK+C+F+EW+ KVVYKRYASLYFC +++ DNEL LE+IH YVE+LD
Sbjct: 33 RELSGVILNRGPKLCNFIEWRGYKVVYKRYASLYFCMCIDEADNELEVLEIIHHYVEILD 92
Query: 439 KYFGXVCELDIIFNFEKAYFILDDWFLGGXLQVNPVK 549
+YFG VCELD+IFNF KAY+ILD+ + G LQ + K
Sbjct: 93 RYFGSVCELDLIFNFHKAYYILDELLIAGELQESSKK 129
Score = 35.1 bits (77), Expect = 0.034
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +2
Query: 164 MMQFMLLFSRQGKLRLQKWY 223
M+ F+LL SRQGK+RL KWY
Sbjct: 1 MIHFVLLVSRQGKVRLTKWY 20
>At4g35410.1 68417.m05029 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 124
Score = 122 bits (294), Expect = 2e-28
Identities = 52/77 (67%), Positives = 63/77 (81%)
Frame = +1
Query: 259 RELITTVLARKPKMCSFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLD 438
REL +L R PK+C+F+EW+ KVVYKRYASLYFC ++QEDNEL LE+IH YVE+LD
Sbjct: 33 RELSGVILNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEIIHHYVEILD 92
Query: 439 KYFGXVCELDIIFNFEK 489
+YFG VCELD+IFNF K
Sbjct: 93 RYFGSVCELDLIFNFHK 109
Score = 35.1 bits (77), Expect = 0.034
Identities = 14/20 (70%), Positives = 17/20 (85%)
Frame = +2
Query: 164 MMQFMLLFSRQGKLRLQKWY 223
M+ F+LL SRQGK+RL KWY
Sbjct: 1 MIHFVLLVSRQGKVRLTKWY 20
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 103 bits (247), Expect = 8e-23
Identities = 43/90 (47%), Positives = 66/90 (73%)
Frame = +1
Query: 262 ELITTVLARKPKMCSFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDK 441
E++ LAR + CSF+E ++ K+VY+RYASL+F ++ ++NEL LE IH VE +DK
Sbjct: 35 EIVRKCLARNDQQCSFVEHRNYKIVYRRYASLFFMVGVDDDENELAILEFIHLLVETMDK 94
Query: 442 YFGXVCELDIIFNFEKAYFILDDWFLGGXL 531
+FG VCELDI+F+ EKA+F+L++ + G +
Sbjct: 95 HFGNVCELDIMFHLEKAHFMLEEMVMNGCI 124
>At1g47830.1 68414.m05324 clathrin coat assembly protein, putative
similar to clathrin coat assembly protein AP17
GB:CAA65533 GI:2959358 from [Zea mays]; contains Pfam
profile: PF01217 clathrin adaptor complex small chain
Length = 142
Score = 98.7 bits (235), Expect = 2e-21
Identities = 45/97 (46%), Positives = 64/97 (65%)
Frame = +1
Query: 244 KEEKTRELITTVLARKPKMCSFLEWKDVKVVYKRYASLYFCCAMEQEDNELLTLELIHRY 423
K + E+ V+ R K +F+E++ KV+Y+RYA L+F ++ DNEL LE IH +
Sbjct: 28 KHKVEYEVHRLVVNRDAKFTNFVEFRTHKVIYRRYAGLFFSVCVDITDNELAYLESIHLF 87
Query: 424 VELLDKYFGXVCELDIIFNFEKAYFILDDWFLGGXLQ 534
VE+LD +F VCELD++FNF K Y ILD++ L G LQ
Sbjct: 88 VEILDHFFSNVCELDLVFNFHKVYLILDEFILAGELQ 124
Score = 30.7 bits (66), Expect = 0.72
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = +2
Query: 164 MMQFMLLFSRQGKLRLQKWYV 226
M++F+LL +RQGK RL K+YV
Sbjct: 1 MIRFILLQNRQGKTRLAKYYV 21
>At3g50860.1 68416.m05569 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 166
Score = 79.4 bits (187), Expect = 2e-15
Identities = 46/101 (45%), Positives = 62/101 (61%), Gaps = 9/101 (8%)
Frame = +1
Query: 250 EKTRELIT---TVLARKPKMCS-FLEWK-----DVKVVYKRYASLYFCCAMEQEDNELLT 402
EK +ELI +VL +P+ S FLE + D ++VYK YA+LYF + +NEL
Sbjct: 26 EKQQELIRGVFSVLCSRPENVSNFLEIESLFGPDSRLVYKHYATLYFVLVFDGSENELAM 85
Query: 403 LELIHRYVELLDKYFGXVCELDIIFNFEKAYFILDDWFLGG 525
L+LI VE LDK F VCELDI+FN+ K + +LD+ GG
Sbjct: 86 LDLIQVLVETLDKCFSNVCELDIVFNYSKMHAVLDEIVFGG 126
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 33.5 bits (73), Expect = 0.10
Identities = 17/62 (27%), Positives = 31/62 (50%)
Frame = +1
Query: 325 VKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEKAYFIL 504
V ++ +++++Y A Q N L +HR V++ YF + E + NF Y +L
Sbjct: 58 VTYMFVQHSNIYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELL 117
Query: 505 DD 510
D+
Sbjct: 118 DE 119
>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 33.1 bits (72), Expect = 0.14
Identities = 17/62 (27%), Positives = 31/62 (50%)
Frame = +1
Query: 325 VKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEKAYFIL 504
V ++ +++++Y A Q N L +HR V++ YF + E + NF Y +L
Sbjct: 58 VTYMFVQHSNVYLMIASRQNCNAASLLFFLHRVVDVFKHYFEELEEESLRDNFVVVYELL 117
Query: 505 DD 510
D+
Sbjct: 118 DE 119
>At4g24550.2 68417.m03519 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 451
Score = 31.1 bits (67), Expect = 0.55
Identities = 19/70 (27%), Positives = 29/70 (41%)
Frame = +1
Query: 325 VKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEKAYFIL 504
V + + LYF + L LEL+ R ++ Y G + E NF Y +L
Sbjct: 57 VNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNFVLVYELL 116
Query: 505 DDWFLGGXLQ 534
D+ G +Q
Sbjct: 117 DEVIDFGYVQ 126
>At4g24550.1 68417.m03518 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 380
Score = 31.1 bits (67), Expect = 0.55
Identities = 19/70 (27%), Positives = 29/70 (41%)
Frame = +1
Query: 325 VKVVYKRYASLYFCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEKAYFIL 504
V + + LYF + L LEL+ R ++ Y G + E NF Y +L
Sbjct: 57 VNYFHVKVVGLYFVATTRVNVSPSLVLELLQRIARVIKDYLGVLNEDSFRKNFVLVYELL 116
Query: 505 DDWFLGGXLQ 534
D+ G +Q
Sbjct: 117 DEVIDFGYVQ 126
>At2g13500.1 68415.m01489 hypothetical protein similar to zinc
finger protein [Arabidopsis thaliana] GI:976277
Length = 338
Score = 27.9 bits (59), Expect = 5.1
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = -1
Query: 393 LIVFLFHSTAEI**CISFIHNFNIFPFKER 304
LI F + I C F HN N +P+++R
Sbjct: 193 LIRFQYERLRRIYSCFRFTHNLNYYPYRQR 222
>At5g11040.1 68418.m01290 expressed protein weak similarity to
hypercellular protein [Aspergillus nidulans] GI:9309269
Length = 1186
Score = 27.5 bits (58), Expect = 6.7
Identities = 16/42 (38%), Positives = 20/42 (47%)
Frame = -2
Query: 539 FTCSXPPKNQSSSMKYAFSKLKMMSSSQTLPKYLSSSST*RW 414
FT PP + SSS +FS+ + TL K L S RW
Sbjct: 963 FTKDPPPGSPSSSRNPSFSEKNTKAEINTLIKNLISKIKVRW 1004
>At1g56590.1 68414.m06508 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 415
Score = 27.1 bits (57), Expect = 8.9
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +1
Query: 361 FCCAMEQEDNELLTLELIHRYVELLDKYFGXVCELDIIFNFEKAYFILDDWFLGG 525
F + E L+ +E + R ++L +Y G + E I NF Y +LD+ G
Sbjct: 67 FLACSQVEMPPLMAIEFLCRVADVLSEYLGGLNEDLIKDNFIIVYELLDEMIDNG 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,478,280
Number of Sequences: 28952
Number of extensions: 214947
Number of successful extensions: 462
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 455
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 462
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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