BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0768 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 3e-09 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 44 1e-05 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 1e-05 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 42 9e-05 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 40 4e-04 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 35 0.008 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 30 0.22 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 27 1.6 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 27 2.1 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 26 4.8 SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 26 4.8 SPAC14C4.15c ||SPAPJ760.01c|dipeptidyl aminopeptidase |Schizosac... 25 6.3 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 25 6.3 SPCC4B3.01 ||SPCP25A2.01c|thiosulfate sulfurtransferase|Schizosa... 25 8.3 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 56.4 bits (130), Expect = 3e-09 Identities = 23/51 (45%), Positives = 34/51 (66%) Frame = +2 Query: 95 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVV 247 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + SD+ + Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI 53 Score = 44.0 bits (99), Expect = 2e-05 Identities = 17/36 (47%), Positives = 27/36 (75%) Frame = +1 Query: 256 DVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 363 DVD+ +IA+E +++MP+F KNG+K++E GAN Sbjct: 56 DVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 44.4 bits (100), Expect = 1e-05 Identities = 19/36 (52%), Positives = 26/36 (72%) Frame = +1 Query: 256 DVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 363 +VDE IAS + +MPTFVF +NGK++D +GAN Sbjct: 59 NVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGAN 94 Score = 34.3 bits (75), Expect = 0.014 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 149 VVIDFMATWCGPCKMIGPKLDEIAAE 226 + +D A WCGPCK I P ++A++ Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASK 47 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 44.4 bits (100), Expect = 1e-05 Identities = 17/44 (38%), Positives = 29/44 (65%) Frame = +2 Query: 95 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 226 ++ D TR++ DK+ V+DF A WCGPCK + P L++++ + Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63 Score = 32.7 bits (71), Expect = 0.042 Identities = 13/37 (35%), Positives = 23/37 (62%) Frame = +1 Query: 256 DVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGANV 366 + D+ DIA + + ++PT V + G++LD GA+V Sbjct: 73 NADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADV 109 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 41.5 bits (93), Expect = 9e-05 Identities = 21/72 (29%), Positives = 37/72 (51%) Frame = +2 Query: 47 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 226 VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67 Query: 227 MSDSIVVVKGTL 262 D V+ G + Sbjct: 68 FEDHNDVLIGKI 79 Score = 30.3 bits (65), Expect = 0.22 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +2 Query: 143 KLVVIDFMATWCGPCKMIGPKLDEI 217 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 Score = 26.6 bits (56), Expect = 2.7 Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Frame = +1 Query: 256 DVDECEDIASEYNINSMPTFV-FVKNGKKLDEFSGA 360 D D D+A +Y+I PT + F +G + ++S A Sbjct: 80 DADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 39.5 bits (88), Expect = 4e-04 Identities = 17/39 (43%), Positives = 26/39 (66%), Gaps = 2/39 (5%) Frame = +2 Query: 143 KLVVIDFMATWCGPCKMIGPKLDEIAAEMSD--SIVVVK 253 K V+++F A WCG CK + P +++A E SD ++VV K Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAK 412 Score = 37.5 bits (83), Expect = 0.001 Identities = 15/39 (38%), Positives = 25/39 (64%), Gaps = 1/39 (2%) Frame = +2 Query: 140 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM-SDSIVVVK 253 DK++++ F A WCG CK + P+ + A E+ D I +V+ Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVE 77 Score = 36.3 bits (80), Expect = 0.003 Identities = 16/36 (44%), Positives = 22/36 (61%) Frame = +1 Query: 250 EGDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSG 357 E D E D+ SEY+I PT KNGK++ ++SG Sbjct: 77 EVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSG 112 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 35.1 bits (77), Expect = 0.008 Identities = 12/53 (22%), Positives = 28/53 (52%) Frame = +2 Query: 83 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 241 MS+ I + + L +++++++F A W PCK + D+ A + +++ Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV 53 Score = 34.3 bits (75), Expect = 0.014 Identities = 13/36 (36%), Positives = 22/36 (61%) Frame = +1 Query: 256 DVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 363 + ++ DIA +++N++P FV + K L SGAN Sbjct: 58 EAEKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 30.3 bits (65), Expect = 0.22 Identities = 24/80 (30%), Positives = 37/80 (46%) Frame = +2 Query: 8 TLSLACFLFAF*TVILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPC 187 TL LACF +F S +I + +S + + + G LVV F A WCG C Sbjct: 10 TLFLACFSLVSG---VFGYSPMFGSNTIEL-NSKNFRKFVKAKGPSLVV--FYAPWCGYC 63 Query: 188 KMIGPKLDEIAAEMSDSIVV 247 K + P ++A+ + + V Sbjct: 64 KKLVPTYQKLASNLHSLLPV 83 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 27.5 bits (58), Expect = 1.6 Identities = 11/32 (34%), Positives = 22/32 (68%) Frame = +1 Query: 253 GDVDECEDIASEYNINSMPTFVFVKNGKKLDE 348 G+VD ++++S NI ++PT +NG+ ++E Sbjct: 83 GEVDCSKELSSCANIRAVPTLYLYQNGEIVEE 114 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 27.1 bits (57), Expect = 2.1 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = +2 Query: 206 LDEIAAEMSDSIVVVKGTLMSARTSP 283 LDE+ M DS+ VVK TL S + P Sbjct: 390 LDEMERSMHDSLSVVKRTLESGKVVP 415 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.8 bits (54), Expect = 4.8 Identities = 15/48 (31%), Positives = 26/48 (54%) Frame = +2 Query: 101 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIV 244 + D + RL EAG + +A CGPC + + D+IA+ ++I+ Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIASGEPNAIL 473 >SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 25.8 bits (54), Expect = 4.8 Identities = 12/54 (22%), Positives = 29/54 (53%), Gaps = 1/54 (1%) Frame = -3 Query: 429 RAAYNFSLYLLVFKDSCFEFVDVSAR-EFVQFLAILNEDERRHRVNVVLAGDVL 271 RA ++ ++L KD+CF +V+A E++ + + D + + + +G ++ Sbjct: 242 RALHSLPKHILPEKDACFYAAEVTAALEYLHLMGFIYRDLKPENILLHQSGHIM 295 >SPAC14C4.15c ||SPAPJ760.01c|dipeptidyl aminopeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 853 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/24 (41%), Positives = 16/24 (66%), Gaps = 1/24 (4%) Frame = +1 Query: 4 LYVVTCVFLIC-ILNCDIIFSFNL 72 +Y+ C+F + +L+C IIF F L Sbjct: 67 IYLAVCLFFLASVLSCAIIFRFYL 90 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = -1 Query: 521 KFITALKTFFIHLLKTVYMFFFFNSILVSI 432 KFI + FF + T Y F F ++L+ + Sbjct: 436 KFILDSQNFFFESINTEYSFIIFTNLLMHL 465 >SPCC4B3.01 ||SPCP25A2.01c|thiosulfate sulfurtransferase|Schizosaccharomyces pombe|chr 3|||Manual Length = 298 Score = 25.0 bits (52), Expect = 8.3 Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 2/59 (3%) Frame = -3 Query: 285 AGDVLALINVPFTTTMESDISA-AISSSLGPII-LHGPHQVAMKSITTSLSPASASLVF 115 +G + IN+PFT T + I+A L + HG ++ IT+ S +AS++F Sbjct: 204 SGHIPTSINIPFTETTAAGITAPKPEEDLEKVFSSHGLTDKSVPIITSCGSGVTASVLF 262 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,240,522 Number of Sequences: 5004 Number of extensions: 41740 Number of successful extensions: 157 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 141 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 157 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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