Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0724
(428 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 64 5e-11
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 64 5e-11
At2g23780.1 68415.m02840 zinc finger (C3HC4-type RING finger) fa... 31 0.33
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 1.0
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 2.3
At1g03050.1 68414.m00277 epsin N-terminal homology (ENTH) domain... 27 4.1
At5g05340.1 68418.m00575 peroxidase, putative similar to peroxid... 27 5.4
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 63.7 bits (148), Expect = 5e-11
Identities = 31/58 (53%), Positives = 33/58 (56%)
Frame = +3
Query: 255 AGHQTSAESWGTGRAVAEFRVXRGGGTHRSXXXXXXXXXXXXXYVRPTKPWRARHRRV 428
AGHQTSAESWGTGRAV+ GGGTHR+ PTK WR HRRV
Sbjct: 64 AGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 121
Score = 58.4 bits (135), Expect = 2e-09
Identities = 30/59 (50%), Positives = 37/59 (62%), Gaps = 2/59 (3%)
Frame = +1
Query: 85 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 255
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKK 63
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 63.7 bits (148), Expect = 5e-11
Identities = 31/58 (53%), Positives = 33/58 (56%)
Frame = +3
Query: 255 AGHQTSAESWGTGRAVAEFRVXRGGGTHRSXXXXXXXXXXXXXYVRPTKPWRARHRRV 428
AGHQTSAESWGTGRAV+ GGGTHR+ PTK WR HRRV
Sbjct: 63 AGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRV 120
Score = 60.5 bits (140), Expect = 5e-10
Identities = 30/62 (48%), Positives = 41/62 (66%), Gaps = 2/62 (3%)
Frame = +1
Query: 76 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 249
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 250 KE 255
K+
Sbjct: 61 KK 62
>At2g23780.1 68415.m02840 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type
Length = 227
Score = 31.1 bits (67), Expect = 0.33
Identities = 14/38 (36%), Positives = 18/38 (47%)
Frame = -3
Query: 387 HTVHXXTCYRRHPDRTYGYHHHGTRGIRQQHVRYPMIR 274
H H T Y P YG +H+G RG+ + PM R
Sbjct: 164 HGFHDATLYGSTPGYPYGGYHNGFRGVPPRGQERPMAR 201
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 1.0
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +1
Query: 100 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 246
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 2.3
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 151 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 246
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 4.1
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 151 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 246
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At1g03050.1 68414.m00277 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to CLATHRIN
COAT ASSEMBLY PROTEIN AP180 - Mus musculus,
SWISSPROT:Q61548
Length = 599
Score = 27.5 bits (58), Expect = 4.1
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 139 PAPSHSSLNTPTLKVGLPIDSFXXFSEAIPPKY 41
PAPS ++ N + +P+D F E PP Y
Sbjct: 519 PAPSTANGNAGNINSPVPMDPFAASLEVAPPAY 551
>At5g05340.1 68418.m00575 peroxidase, putative similar to peroxidase
[Nicotiana tabacum] gi|5381253|dbj|BAA82306; similar to
Peroxidase P7 [Brassica rapa (Turnip)] SWISS-PROT:P00434
Length = 324
Score = 27.1 bits (57), Expect = 5.4
Identities = 15/46 (32%), Positives = 21/46 (45%)
Frame = +3
Query: 75 NESIGSPTFSVGVFREE*DGAGCSQAPPVRIQGAHTSGPGQ*CSRF 212
N +I +PT S+ G S V + GAHT G + C+ F
Sbjct: 163 NSNIPAPTSSLSQLISSFSAVGLSTRDMVALSGAHTIGQSR-CTNF 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,634,290
Number of Sequences: 28952
Number of extensions: 170219
Number of successful extensions: 438
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 420
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 436
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 675111616
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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