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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0721
         (421 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g22100.1 68418.m02573 RNA cyclase family protein contains Pfa...    35   0.026
At3g03950.2 68416.m00414 expressed protein contains Pfam profile...    30   0.73 
At1g58430.1 68414.m06647 GDSL-motif lipase/hydrolase family prot...    27   3.9  
At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase...    26   9.0  

>At5g22100.1 68418.m02573 RNA cyclase family protein contains Pfam
           profiles: PF01137 RNA 3'-terminal phosphate cyclase,
           PF05189 RNA 3-prime-terminal phosphate cyclase (RTC)
          Length = 375

 Score = 34.7 bits (76), Expect = 0.026
 Identities = 20/68 (29%), Positives = 30/68 (44%), Gaps = 3/68 (4%)
 Frame = +1

Query: 31  IDYMTEVFKHAVKHFGV---DFNLTIHRRGYFPKGGGHVTVEVSPLRHVQAADVTRGGEV 201
           +D       + +K FGV   D  L I  RG  P GGG V + V  ++ + A      G V
Sbjct: 127 VDTFRSTTLNIIKRFGVPAEDLELKIEARGVAPNGGGEVLLTVPNIKTLSAVHWVEEGMV 186

Query: 202 AAVYGWSY 225
             + G ++
Sbjct: 187 KKIRGTTF 194


>At3g03950.2 68416.m00414 expressed protein contains Pfam profile
           PF04146: YT521-B-like family
          Length = 424

 Score = 29.9 bits (64), Expect = 0.73
 Identities = 21/74 (28%), Positives = 29/74 (39%)
 Frame = +1

Query: 79  VDFNLTIHRRGYFPKGGGHVTVEVSPLRHVQAADVTRGGEVAAVYGWSYVAGCLPIKMAT 258
           V  N T H  G+ P G      E  P  ++ A ++  GG+    Y W Y+    P     
Sbjct: 35  VPMNFTKHPYGHAPYGASSHGSERRP--NMNAGNLLNGGDSIGSYPWGYIPANYPSGGYP 92

Query: 259 QWRMVLRRNSAGSA 300
             R    RNS  S+
Sbjct: 93  DPRFGYDRNSNHSS 106


>At1g58430.1 68414.m06647 GDSL-motif lipase/hydrolase family protein
           similar to family II lipases EXL3 GI:15054386, EXL1
           GI:15054382, EXL2 GI:15054384 from [Arabidopsis
           thaliana]; contains Pfam profile PF00657: GDSL-like
           Lipase/Acylhydrolase
          Length = 360

 Score = 27.5 bits (58), Expect = 3.9
 Identities = 11/16 (68%), Positives = 13/16 (81%)
 Frame = +1

Query: 232 GCLPIKMATQWRMVLR 279
           GCLPI+M  Q+R VLR
Sbjct: 229 GCLPIQMTAQFRNVLR 244


>At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase C
           (PLC1) identical to phosphoinositide specific
           phospholipase C [Arabidopsis thaliana] GI:902923
          Length = 561

 Score = 26.2 bits (55), Expect = 9.0
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +1

Query: 16  EMAPQIDYMTEVFKHAVKHFGVDFNLTIHRRGYF 117
           E    I+Y+ ++F H +KH GV     IH  G++
Sbjct: 54  ETHADINYVKDIF-HRLKHHGVFHPRGIHLEGFY 86


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,630,242
Number of Sequences: 28952
Number of extensions: 189342
Number of successful extensions: 370
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 367
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 370
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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