BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0721 (421 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g22100.1 68418.m02573 RNA cyclase family protein contains Pfa... 35 0.026 At3g03950.2 68416.m00414 expressed protein contains Pfam profile... 30 0.73 At1g58430.1 68414.m06647 GDSL-motif lipase/hydrolase family prot... 27 3.9 At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase... 26 9.0 >At5g22100.1 68418.m02573 RNA cyclase family protein contains Pfam profiles: PF01137 RNA 3'-terminal phosphate cyclase, PF05189 RNA 3-prime-terminal phosphate cyclase (RTC) Length = 375 Score = 34.7 bits (76), Expect = 0.026 Identities = 20/68 (29%), Positives = 30/68 (44%), Gaps = 3/68 (4%) Frame = +1 Query: 31 IDYMTEVFKHAVKHFGV---DFNLTIHRRGYFPKGGGHVTVEVSPLRHVQAADVTRGGEV 201 +D + +K FGV D L I RG P GGG V + V ++ + A G V Sbjct: 127 VDTFRSTTLNIIKRFGVPAEDLELKIEARGVAPNGGGEVLLTVPNIKTLSAVHWVEEGMV 186 Query: 202 AAVYGWSY 225 + G ++ Sbjct: 187 KKIRGTTF 194 >At3g03950.2 68416.m00414 expressed protein contains Pfam profile PF04146: YT521-B-like family Length = 424 Score = 29.9 bits (64), Expect = 0.73 Identities = 21/74 (28%), Positives = 29/74 (39%) Frame = +1 Query: 79 VDFNLTIHRRGYFPKGGGHVTVEVSPLRHVQAADVTRGGEVAAVYGWSYVAGCLPIKMAT 258 V N T H G+ P G E P ++ A ++ GG+ Y W Y+ P Sbjct: 35 VPMNFTKHPYGHAPYGASSHGSERRP--NMNAGNLLNGGDSIGSYPWGYIPANYPSGGYP 92 Query: 259 QWRMVLRRNSAGSA 300 R RNS S+ Sbjct: 93 DPRFGYDRNSNHSS 106 >At1g58430.1 68414.m06647 GDSL-motif lipase/hydrolase family protein similar to family II lipases EXL3 GI:15054386, EXL1 GI:15054382, EXL2 GI:15054384 from [Arabidopsis thaliana]; contains Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase Length = 360 Score = 27.5 bits (58), Expect = 3.9 Identities = 11/16 (68%), Positives = 13/16 (81%) Frame = +1 Query: 232 GCLPIKMATQWRMVLR 279 GCLPI+M Q+R VLR Sbjct: 229 GCLPIQMTAQFRNVLR 244 >At5g58670.1 68418.m07351 phosphoinositide-specific phospholipase C (PLC1) identical to phosphoinositide specific phospholipase C [Arabidopsis thaliana] GI:902923 Length = 561 Score = 26.2 bits (55), Expect = 9.0 Identities = 12/34 (35%), Positives = 19/34 (55%) Frame = +1 Query: 16 EMAPQIDYMTEVFKHAVKHFGVDFNLTIHRRGYF 117 E I+Y+ ++F H +KH GV IH G++ Sbjct: 54 ETHADINYVKDIF-HRLKHHGVFHPRGIHLEGFY 86 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,630,242 Number of Sequences: 28952 Number of extensions: 189342 Number of successful extensions: 370 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 367 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 370 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 645327280 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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