BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0715
(432 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58070.1 68418.m07267 lipocalin, putative similar to temperat... 40 5e-04
At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 31 0.44
At1g59560.1 68414.m06698 expressed protein contains similarity t... 30 0.78
At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor, pu... 29 1.0
At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative s... 28 2.4
At5g49110.1 68418.m06079 expressed protein ; expression support... 27 7.2
At5g37100.1 68418.m04453 replication protein-related weak simila... 27 7.2
At4g11110.1 68417.m01803 WD-40 repeat family protein / phytochro... 27 7.2
At2g14800.1 68415.m01677 hypothetical protein 27 7.2
At3g05150.1 68416.m00559 sugar transporter family protein simila... 26 9.6
>At5g58070.1 68418.m07267 lipocalin, putative similar to temperature
stress-induced lipocalin [Triticum aestivum] GI:18650668
Length = 186
Score = 40.3 bits (90), Expect = 5e-04
Identities = 23/64 (35%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = +3
Query: 90 ELKPVNNFNLTAYQGIWYEISKFPNESE-KNGKCSSAEYKLEGD-VVKVKNVHIIDGSRS 263
E++ V N+ Y G WYEI+ FP+ + KNG + A Y L D + V N +G R
Sbjct: 6 EMEVVKGLNVERYMGRWYEIASFPSRFQPKNGVDTRATYTLNPDGTIHVLNETWSNGKRG 65
Query: 264 I*KG 275
+G
Sbjct: 66 FIEG 69
>At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative
strong similarity to probable NADP-dependent
oxidoreductase (zeta-crystallin homolog) P1
[SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430],
Arabidopsis thaliana
Length = 345
Score = 30.7 bits (66), Expect = 0.44
Identities = 19/55 (34%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
Frame = +3
Query: 147 ISKFPNESEKNGKCSSAEYKLEG--DVVKVKNVHI-IDGSRSI*KGRPSSPTTPI 302
+S FP ES+ + K ++ E+KL G + V VKN+++ D I G+P T +
Sbjct: 14 VSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYMRIRMGKPDPSTAAL 68
>At1g59560.1 68414.m06698 expressed protein contains similarity to
apoptosis inhibitors
Length = 338
Score = 29.9 bits (64), Expect = 0.78
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
Frame = +1
Query: 169 LRRTANALQLNTNWKVTW*RSRTCISSTAQEV--YRRDG 279
L+RTA L NW+ +W R+ T + +EV Y DG
Sbjct: 78 LKRTAEQQVLRRNWRFSWVRNSTLMQPMTKEVPWYLDDG 116
>At1g08550.1 68414.m00948 violaxanthin de-epoxidase precursor,
putative (AVDE1) similar to EST gb|N37612
Length = 462
Score = 29.5 bits (63), Expect = 1.0
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = +3
Query: 102 VNNFNLTAYQGIWYEISKFPNESEKNGKCSSAEYKLEGDVVKVKNV 239
V NFN++ + G WY I+ N + C E+ EGD V N+
Sbjct: 204 VQNFNISDFNGKWY-ITSGLNPTFDAFDCQLHEFHTEGDNKLVGNI 248
>At5g60040.1 68418.m07529 DNA-directed RNA polymerase, putative
similar to SP|P04051 DNA-directed RNA polymerase III
largest subunit (EC 2.7.7.6) {Saccharomyces cerevisiae};
contains InterPro accession IPR000722: RNA polymerase,
alpha subunit
Length = 1328
Score = 28.3 bits (60), Expect = 2.4
Identities = 13/46 (28%), Positives = 21/46 (45%)
Frame = +2
Query: 263 YIEGTAKLTDDANKAAKLTVTFKFGEISRDGSVQVLATDYNNYAIA 400
Y + ++ KA F G ++DG +L DYN++A A
Sbjct: 609 YFRNSELISGQLGKATLALDIFPLGNGNKDGLYSILLRDYNSHAAA 654
>At5g49110.1 68418.m06079 expressed protein ; expression supported
by MPSS
Length = 1487
Score = 26.6 bits (56), Expect = 7.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +2
Query: 329 KFGEISRDGSVQVLATDYNNYAIAYNCKY 415
KFGE S L T YN+Y ++ +CK+
Sbjct: 321 KFGESSLGMLRTALLTAYNDYRLSKDCKW 349
>At5g37100.1 68418.m04453 replication protein-related weak
similarity to Replication Protein A 70 kDa DNA-binding
subunit (RP-A) (RF-A) (Replication factor-A protein 1)
(Single-stranded DNA-binding protein) (DMRPA1)
(GI:2498844)(SP:Q24492) [Drosophila melanogaster]
Length = 269
Score = 26.6 bits (56), Expect = 7.2
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +3
Query: 102 VNNFNLTAYQGIWYEISKFP-NESEKNGKCSSAEYKLE 212
+NNF + ++G WY I+ F + K SS Y++E
Sbjct: 60 LNNFKPSLFEGKWYYIADFRLKRATSIPKYSSFNYEIE 97
>At4g11110.1 68417.m01803 WD-40 repeat family protein / phytochrome
A-related contains 7 WD-40 repeats (PF00400); similar to
phytochrome A supressor spa1 (GI:4809171) [Arabidopsis
thaliana]; contains non-consensus (GC) donor splice
sites at introns 4 and 6
Length = 1017
Score = 26.6 bits (56), Expect = 7.2
Identities = 17/38 (44%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = +3
Query: 177 NGKCSSAEYKL-EGDVVKVKNVHIIDGSRSI*KGRPSS 287
N CSS + EGDVV+ V +GS GRPSS
Sbjct: 77 NEPCSSRQNTNDEGDVVEELTVKTCEGSSMAIVGRPSS 114
>At2g14800.1 68415.m01677 hypothetical protein
Length = 580
Score = 26.6 bits (56), Expect = 7.2
Identities = 17/47 (36%), Positives = 20/47 (42%), Gaps = 2/47 (4%)
Frame = +1
Query: 130 KASGMKSRNFPTNLRRTANALQLNTNWKVTW*RSRTC--ISSTAQEV 264
K G+KS F N + + N W TW R TC I S EV
Sbjct: 175 KLKGLKSWMFKYNFEKRILEKRHNRGWLHTWSRRVTCRKIKSAKLEV 221
>At3g05150.1 68416.m00559 sugar transporter family protein similar
to sugar-porter family proteins 1 and 2 [Arabidopsis
thaliana] GI:14585699, GI:14585701; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 470
Score = 26.2 bits (55), Expect = 9.6
Identities = 11/25 (44%), Positives = 13/25 (52%)
Frame = +1
Query: 148 SRNFPTNLRRTANALQLNTNWKVTW 222
S FP NL+ TA L NW +W
Sbjct: 394 SEIFPINLKGTAGGLVTVVNWLSSW 418
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,103,596
Number of Sequences: 28952
Number of extensions: 145230
Number of successful extensions: 397
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 388
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 397
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 685039728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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