BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0684
(428 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 29 0.40
SPBC2D10.08c |||mitochondrial ribosomal protein subunit Yml6|Sch... 26 2.2
SPBC30B4.03c |||conserved protein |Schizosaccharomyces pombe|chr... 25 5.0
SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyce... 25 6.6
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr... 25 6.6
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos... 25 6.6
SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase Rdp1|Schizosa... 25 6.6
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 24 8.7
SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 24 8.7
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 28.7 bits (61), Expect = 0.40
Identities = 13/49 (26%), Positives = 23/49 (46%), Gaps = 1/49 (2%)
Frame = -3
Query: 273 CCPRIHRHFNS-SQ*QRTRNFGKHSIDTAASTSTCHTLQTSTLKSLLII 130
C +H H N+ Q +NFG + + T + STC +K + ++
Sbjct: 240 CIATLHGHKNTIMQASFQKNFGSNYLATVSRDSTCRVFDLRAMKDVRVL 288
>SPBC2D10.08c |||mitochondrial ribosomal protein subunit
Yml6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 26.2 bits (55), Expect = 2.2
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = -1
Query: 278 LYAVHVSTGISTRHNNNELEILVNIASILQHPQVRV 171
+Y + + +STR NNEL IL N ++ Q P+ R+
Sbjct: 110 VYNLAMRIALSTRFINNELTILENPINLSQ-PKTRI 144
>SPBC30B4.03c |||conserved protein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 25.0 bits (52), Expect = 5.0
Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Frame = +2
Query: 209 LPKFLVRCYCDEL-KCRWIRGQHKEF 283
LP+FL YC +L K ++ G KEF
Sbjct: 84 LPRFLYLSYCGKLKKMSFLLGNTKEF 109
>SPAC30D11.10 |rad22||DNA repair protein Rad22|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 469
Score = 24.6 bits (51), Expect = 6.6
Identities = 10/33 (30%), Positives = 17/33 (51%)
Frame = -1
Query: 119 SRRNNRPKAHDQLNNNEKAKHRSTKRNPKKISY 21
+ ++ KA Q+NN +K H S K + +Y
Sbjct: 267 ANNHHSEKAGTQINNKDKGSHNSAKPVQRSHTY 299
>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 652
Score = 24.6 bits (51), Expect = 6.6
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = -1
Query: 125 LKSRRNNRPKAHDQLNNNEKAKHRSTKRNPKKISYVRAT 9
L++R +A Q +N++ + K+ KK+ +RAT
Sbjct: 113 LEARLKGDKEARGQNSNDQPEESDDKKKRKKKVKEIRAT 151
>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 449
Score = 24.6 bits (51), Expect = 6.6
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 166 CVTRTCGCCSIDAMFTKISSS 228
C TC C+ID MF+ I +S
Sbjct: 183 CKRPTCMTCAIDDMFSSIYNS 203
>SPAC6F12.09 |rdp1|rdr1|RNA-directed RNA polymerase
Rdp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1215
Score = 24.6 bits (51), Expect = 6.6
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = -1
Query: 113 RNNRPKAHDQLNNNEKAKHRSTKRN 39
RN + K ++NNE H + KRN
Sbjct: 30 RNKKQKKWASVHNNEAQLHSANKRN 54
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 24.2 bits (50), Expect = 8.7
Identities = 10/28 (35%), Positives = 14/28 (50%)
Frame = -1
Query: 125 LKSRRNNRPKAHDQLNNNEKAKHRSTKR 42
L+ R +Q++NN HR TKR
Sbjct: 114 LEEIRKRNQSRKNQMSNNSTTYHRETKR 141
>SPCC1322.12c |bub1||serine/threonine protein kinase
Bub1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1044
Score = 24.2 bits (50), Expect = 8.7
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = -1
Query: 101 PKAHDQLNNNEKAKHRSTKRNP 36
PK + +N N+ KH S+ NP
Sbjct: 562 PKGYMFVNENQSMKHESSVSNP 583
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,751,381
Number of Sequences: 5004
Number of extensions: 34226
Number of successful extensions: 76
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 76
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 76
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 154448264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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