Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0673
(421 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g31160.1 68414.m03812 zinc-binding protein, putative / protei... 77 5e-15
At3g56490.1 68416.m06282 zinc-binding protein, putative / protei... 69 1e-12
At4g16566.1 68417.m02507 histidine triad family protein / HIT fa... 49 1e-06
At5g48545.1 68418.m06002 histidine triad family protein / HIT fa... 46 1e-05
At5g58240.2 68418.m07291 bis(5'-adenosyl)-triphosphatase, putati... 31 0.24
At5g58240.1 68418.m07292 bis(5'-adenosyl)-triphosphatase, putati... 31 0.24
At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransfera... 30 0.55
At2g26780.1 68415.m03212 expressed protein contains Pfam profile... 29 1.7
At2g38840.1 68415.m04772 guanylate-binding family protein simila... 27 3.9
At3g04960.1 68416.m00538 expressed protein low similarity to SP|... 27 5.2
At5g54450.1 68418.m06781 hypothetical protein contains Pfam prof... 26 9.0
>At1g31160.1 68414.m03812 zinc-binding protein, putative / protein
kinase C inhibitor, putative similar to 14 kDa
zinc-binding protein (Protein kinase C inhibitor, PKCI)
[Zea mays] Swiss-Prot:P42856
Length = 187
Score = 77.0 bits (181), Expect = 5e-15
Identities = 39/80 (48%), Positives = 51/80 (63%), Gaps = 2/80 (2%)
Frame = +1
Query: 19 AQTAAPGGDTIFGKILRKEIPANFIYEDEQCVAFNDVNPQAPTHVLVIP--RKPIPQLSL 192
A A G TIF KI+ KEIP++ +YEDE +AF D+NPQAP HVLVIP R + L
Sbjct: 67 ASVADTGAPTIFDKIIAKEIPSDIVYEDENVLAFRDINPQAPVHVLVIPKLRDGLTSLGK 126
Query: 193 ADDTDEQLLGHLLIVARKVA 252
A+ ++LG LL ++ VA
Sbjct: 127 AEPRHVEVLGQLLHASKIVA 146
Score = 65.3 bits (152), Expect = 2e-11
Identities = 24/34 (70%), Positives = 30/34 (88%)
Frame = +3
Query: 276 GFRLVVNDGKNGAQSVYHLHIHILGGRQMQWPPG 377
GFR+V+N+G QSVYHLH+H+LGGRQM+WPPG
Sbjct: 154 GFRVVINNGVEACQSVYHLHLHVLGGRQMKWPPG 187
>At3g56490.1 68416.m06282 zinc-binding protein, putative / protein
kinase C inhibitor, putative similar to 14 kDa
zinc-binding protein (Protein kinase C inhibitor, PKCI)
[Zea mays] Swiss-Prot:P42856
Length = 147
Score = 69.3 bits (162), Expect = 1e-12
Identities = 36/85 (42%), Positives = 52/85 (61%), Gaps = 3/85 (3%)
Frame = +1
Query: 7 EVKLAQTAAPGGD-TIFGKILRKEIPANFIYEDEQCVAFNDVNPQAPTHVLVIP--RKPI 177
E + A A P TIF KI+ KEIP+ ++ED++ +AF D+ PQ P H+L+IP R +
Sbjct: 22 EKEAALAATPSDSPTIFDKIISKEIPSTVVFEDDKVLAFRDITPQGPVHILLIPKVRDGL 81
Query: 178 PQLSLADDTDEQLLGHLLIVARKVA 252
LS A++ +LG LL A+ VA
Sbjct: 82 TGLSKAEERHIDILGRLLYTAKLVA 106
Score = 68.5 bits (160), Expect = 2e-12
Identities = 24/34 (70%), Positives = 30/34 (88%)
Frame = +3
Query: 276 GFRLVVNDGKNGAQSVYHLHIHILGGRQMQWPPG 377
GFR+V+NDG G QSVYH+H+H++GGRQM WPPG
Sbjct: 114 GFRIVINDGPQGCQSVYHIHVHLIGGRQMNWPPG 147
>At4g16566.1 68417.m02507 histidine triad family protein / HIT
family protein contains Pfam domain, PF01230: HIT family
(histidine triad protein family)
Length = 146
Score = 48.8 bits (111), Expect = 1e-06
Identities = 24/69 (34%), Positives = 40/69 (57%), Gaps = 2/69 (2%)
Frame = +1
Query: 49 IFGKILRKEIPANFIYEDEQCVAFNDVNPQAPTHVLVIPRKPIPQLS--LADDTDEQLLG 222
IF +I+R ++ DE+ +AF D+ P A H LVIP++ IP ++ D D L+
Sbjct: 9 IFCEIVRNPTTTRLLHTDEKVIAFQDIKPAAQRHYLVIPKEHIPTVNDLQRRDEDYSLVR 68
Query: 223 HLLIVARKV 249
H+L V +++
Sbjct: 69 HMLSVGQQL 77
>At5g48545.1 68418.m06002 histidine triad family protein / HIT
family protein contains Pfam profile PF01230:HIT domain;
contains Prosite motif PS00892: HIT family signature.
Length = 197
Score = 45.6 bits (103), Expect = 1e-05
Identities = 20/48 (41%), Positives = 28/48 (58%)
Frame = +1
Query: 43 DTIFGKILRKEIPANFIYEDEQCVAFNDVNPQAPTHVLVIPRKPIPQL 186
D +F KI+R E P +YED+ C+ D NP + H L+IP+ P L
Sbjct: 49 DCVFCKIIRGESPCLKLYEDDMCLCILDTNPLSHGHSLIIPKLHYPTL 96
Score = 31.1 bits (67), Expect = 0.32
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +3
Query: 279 FRLVVNDGKNGAQSVYHLHIHIL 347
F L+VN+G Q ++H HIHI+
Sbjct: 127 FNLLVNNGAAAGQVIFHTHIHII 149
>At5g58240.2 68418.m07291 bis(5'-adenosyl)-triphosphatase, putative
similar to bis(5'-adenosyl)-triphosphatase (Diadenosine
5',5'''- P1,P3-triphosphate hydrolase,
Dinucleosidetriphosphatase, AP3A hydrolase, AP3AASE,
Fragile histidine triad protein) [Homo sapiens]
Swiss-Prot:P49789
Length = 160
Score = 31.5 bits (68), Expect = 0.24
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 2/77 (2%)
Frame = +1
Query: 79 PANFIYEDEQCVAFNDVNPQAPTHVLVIPRKPIPQLS--LADDTDEQLLGHLLIVARKVA 252
P Y A ++ P P HVLV PR+ +P+ + AD+T + L + A+KV
Sbjct: 17 PREVFYATPLSYAMVNLRPLLPAHVLVCPRRLVPRFTDLTADETSD-----LWLTAQKVG 71
Query: 253 AS*AWTRQASA*LLTME 303
+ AS+ L ++
Sbjct: 72 SKLETFHNASSLTLAIQ 88
Score = 29.1 bits (62), Expect = 1.3
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 285 LVVNDGKNGAQSVYHLHIHIL 347
L + DG Q+V H+HIHIL
Sbjct: 85 LAIQDGPQAGQTVPHVHIHIL 105
>At5g58240.1 68418.m07292 bis(5'-adenosyl)-triphosphatase, putative
similar to bis(5'-adenosyl)-triphosphatase (Diadenosine
5',5'''- P1,P3-triphosphate hydrolase,
Dinucleosidetriphosphatase, AP3A hydrolase, AP3AASE,
Fragile histidine triad protein) [Homo sapiens]
Swiss-Prot:P49789
Length = 180
Score = 31.5 bits (68), Expect = 0.24
Identities = 22/77 (28%), Positives = 36/77 (46%), Gaps = 2/77 (2%)
Frame = +1
Query: 79 PANFIYEDEQCVAFNDVNPQAPTHVLVIPRKPIPQLS--LADDTDEQLLGHLLIVARKVA 252
P Y A ++ P P HVLV PR+ +P+ + AD+T + L + A+KV
Sbjct: 37 PREVFYATPLSYAMVNLRPLLPAHVLVCPRRLVPRFTDLTADETSD-----LWLTAQKVG 91
Query: 253 AS*AWTRQASA*LLTME 303
+ AS+ L ++
Sbjct: 92 SKLETFHNASSLTLAIQ 108
Score = 29.1 bits (62), Expect = 1.3
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +3
Query: 285 LVVNDGKNGAQSVYHLHIHIL 347
L + DG Q+V H+HIHIL
Sbjct: 105 LAIQDGPQAGQTVPHVHIHIL 125
>At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransferase,
putative similar to polynucleotide phosphorylase
GB:AAC50039 [Pisum sativum], identical to putative
polynucleotide phosphorylase GB:AAF00646 [Arabidopsis
thaliana]
Length = 922
Score = 30.3 bits (65), Expect = 0.55
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = +1
Query: 97 EDEQCVAFNDVNPQAPTHVLVIPRKPIPQL 186
EDE+ V +V+ Q H+ IPRKPIP L
Sbjct: 391 EDEEVVPEGEVD-QGDVHIRPIPRKPIPLL 419
>At2g26780.1 68415.m03212 expressed protein contains Pfam profile TBP
(TATA-binding protein) -interacting protein 120 (TIP120);
contains TIGRFAM profile TIGR01612: reticulocyte binding
protein
Length = 1866
Score = 28.7 bits (61), Expect = 1.7
Identities = 19/56 (33%), Positives = 26/56 (46%)
Frame = -3
Query: 173 GFLGITNT*VGACGFTSLNATHCSSS*MKFAGISLRNIFPKIVSPPGAAVCASFTS 6
G +G+ NT VG F SL S F G+ LR +FP +A +F+S
Sbjct: 1387 GGVGL-NTRVGVASFISLLVQKVGSEIKPFTGMLLRLLFPVAKEEKSSAAKRAFSS 1441
>At2g38840.1 68415.m04772 guanylate-binding family protein similar
to SP|Q01514 Interferon-induced guanylate-binding
protein 1 (Guanine nucleotide-binding protein 1)
(Interferon-gamma inducible protein MAG-1) {Mus
musculus}; contains Pfam profile PF02263:
Guanylate-binding protein, N-terminal domain
Length = 679
Score = 27.5 bits (58), Expect = 3.9
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = +2
Query: 140 LLLMYW*YQGSLFHSSRWLMTLMNS 214
L ++YW G H +RWL+ L N+
Sbjct: 644 LCVLYWRCYGKRKHGARWLLPLYNN 668
>At3g04960.1 68416.m00538 expressed protein low similarity to
SP|P32380 NUF1 protein (Spindle poly body spacer protein
SPC110) {Saccharomyces cerevisiae}
Length = 556
Score = 27.1 bits (57), Expect = 5.2
Identities = 8/22 (36%), Positives = 16/22 (72%)
Frame = -1
Query: 70 YVIFSQRLYHRLERQFVQVSPR 5
+++FS +YH + Q+V ++PR
Sbjct: 406 HLMFSHEMYHTIRGQYVTINPR 427
>At5g54450.1 68418.m06781 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 368
Score = 26.2 bits (55), Expect = 9.0
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = +3
Query: 9 GETCTNCRSRR*YNLWENIT*RNPCKFHLR 98
G + CR R ++ W NI NPC F R
Sbjct: 160 GPQLSFCRPARSHSDWTNIRIENPCFFSSR 189
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,668,544
Number of Sequences: 28952
Number of extensions: 194507
Number of successful extensions: 425
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 425
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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