Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0671
(398 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 87 4e-18
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 87 4e-18
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 87 4e-18
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 32 0.12
At1g29760.1 68414.m03639 expressed protein 31 0.37
At1g35530.1 68414.m04411 DEAD/DEAH box helicase, putative low si... 29 0.86
At1g32550.1 68414.m04017 ferredoxin family protein similar to fe... 29 1.1
At4g38440.1 68417.m05432 expressed protein 27 4.6
At1g71110.1 68414.m08206 expressed protein 27 4.6
At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot... 27 6.1
At2g30210.1 68415.m03674 laccase, putative / diphenol oxidase, p... 27 6.1
At5g11810.1 68418.m01378 expressed protein 26 8.0
At5g07280.1 68418.m00830 leucine-rich repeat protein kinase, put... 26 8.0
At3g53540.1 68416.m05912 expressed protein 26 8.0
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 87.0 bits (206), Expect = 4e-18
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +3
Query: 21 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL 152
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPL
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPL 44
Score = 80.6 bits (190), Expect = 4e-16
Identities = 36/46 (78%), Positives = 39/46 (84%)
Frame = +1
Query: 259 TYPAGFMDVVSIXKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKV 396
TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV
Sbjct: 81 TYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKV 126
Score = 50.4 bits (115), Expect = 4e-07
Identities = 26/44 (59%), Positives = 32/44 (72%), Gaps = 1/44 (2%)
Frame = +2
Query: 125 PQVARVLAP-VIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRT 253
P +R P V+ +RNRLKYALT EV+ I+ QR I+VDGKVRT
Sbjct: 35 PHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDGKVRT 78
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 87.0 bits (206), Expect = 4e-18
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +3
Query: 21 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL 152
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPL
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPL 44
Score = 80.6 bits (190), Expect = 4e-16
Identities = 36/46 (78%), Positives = 39/46 (84%)
Frame = +1
Query: 259 TYPAGFMDVVSIXKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKV 396
TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV
Sbjct: 81 TYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKV 126
Score = 50.4 bits (115), Expect = 4e-07
Identities = 26/44 (59%), Positives = 32/44 (72%), Gaps = 1/44 (2%)
Frame = +2
Query: 125 PQVARVLAP-VIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRT 253
P +R P V+ +RNRLKYALT EV+ I+ QR I+VDGKVRT
Sbjct: 35 PHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDGKVRT 78
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 87.0 bits (206), Expect = 4e-18
Identities = 37/44 (84%), Positives = 40/44 (90%)
Frame = +3
Query: 21 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPL 152
MARG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPL
Sbjct: 1 MARGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPL 44
Score = 80.6 bits (190), Expect = 4e-16
Identities = 36/46 (78%), Positives = 39/46 (84%)
Frame = +1
Query: 259 TYPAGFMDVVSIXKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKV 396
TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV
Sbjct: 81 TYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKV 126
Score = 50.4 bits (115), Expect = 4e-07
Identities = 26/44 (59%), Positives = 32/44 (72%), Gaps = 1/44 (2%)
Frame = +2
Query: 125 PQVARVLAP-VIFLRNRLKYALTGNEVLKIVKQRLIKVDGKVRT 253
P +R P V+ +RNRLKYALT EV+ I+ QR I+VDGKVRT
Sbjct: 35 PHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDGKVRT 78
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 32.3 bits (70), Expect = 0.12
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 154 HRGKHSRNLWGPVDGLGAYTPPSLSNIHAL 65
H KHS +LWG D L TPP+ ++ L
Sbjct: 41 HWKKHSADLWGSADALAIATPPASDDLRYL 70
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 30.7 bits (66), Expect = 0.37
Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -3
Query: 255 AVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL-RKITGAS 142
++LTFP +R CF F F + RRFL ++ G S
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFLMARVAGIS 233
>At1g35530.1 68414.m04411 DEAD/DEAH box helicase, putative low
similarity to RNA helicase/RNAseIII CAF protein
[Arabidopsis thaliana] GI:6102610; contains Pfam
profiles PF00270: DEAD/DEAH box helicase, PF00271:
Helicase conserved C-terminal domain
Length = 1324
Score = 29.5 bits (63), Expect = 0.86
Identities = 17/54 (31%), Positives = 25/54 (46%)
Frame = -2
Query: 175 QTIPQENHRGKHSRNLWGPVDGLGAYTPPSLSNIHALGAFKRFKCFLGPRAMLD 14
QT+P + H+ HSR +D + P+ S + F F+ LG R LD
Sbjct: 647 QTLPSKVHKVMHSRQTGMLIDAMQHLQEPTFSE-QSKSFFTEFRAPLGEREELD 699
>At1g32550.1 68414.m04017 ferredoxin family protein similar to
ferredoxin from Synechocystis sp. [GI:48019]; contains
Pfam profile PF00111 2Fe-2S iron-sulfur cluster binding
domain
Length = 181
Score = 29.1 bits (62), Expect = 1.1
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = -3
Query: 249 LTFPSTFMRRCFTIFRTSFPVKAYFRRFLRKITGASTRATCGDRLTVSVHT 97
L P TF C ++ + +FP+ RR++ +T ATC R+ V V T
Sbjct: 3 LILPCTF---CTSLQKKNFPIN---RRYITNFRRGATTATCEFRIPVEVST 47
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 27.1 bits (57), Expect = 4.6
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 48 KRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECL 146
+R+ P W L + V++ + STGP + E L
Sbjct: 1140 QRMPLPPHWFLSAISAVHSGKTSTGPPESTELL 1172
>At1g71110.1 68414.m08206 expressed protein
Length = 513
Score = 27.1 bits (57), Expect = 4.6
Identities = 15/68 (22%), Positives = 25/68 (36%)
Frame = +1
Query: 187 DRKRSPENCETTPHKG*WES*DCPTYPAGFMDVVSIXKTNELFRLIYDVKGRFTIHRITP 366
DR+ SP WE+ C P+G V + +L+ V + + TP
Sbjct: 358 DRQCSPWELSIENASSVWENYKCEVTPSGICTTVGRVTPDTFGQLVAAVNESYALEHYTP 417
Query: 367 EEAKYKLC 390
++ C
Sbjct: 418 PLLSFRDC 425
>At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein
similar to lipase [Arabidopsis thaliana] GI:1145627;
contains Pfam profile:PF00657 Lipase/Acylhydrolase with
GDSL-like motif
Length = 323
Score = 26.6 bits (56), Expect = 6.1
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = -3
Query: 240 PSTFMRRCFTIFRTSFPVK 184
P F CF I+ TSFPVK
Sbjct: 175 PGNFPVGCFPIYLTSFPVK 193
>At2g30210.1 68415.m03674 laccase, putative / diphenol oxidase,
putative similar to laccase [Populus balsamifera subsp.
trichocarpa][GI:3805960]
Length = 570
Score = 26.6 bits (56), Expect = 6.1
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -2
Query: 163 QENHRGKHSRNLWGPVDGLGAYTPPSLSNI 74
Q ++ G SR LW P+ G AY SN+
Sbjct: 427 QFDYTGNVSRGLWQPIKGTKAYKLKYKSNV 456
>At5g11810.1 68418.m01378 expressed protein
Length = 306
Score = 26.2 bits (55), Expect = 8.0
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -2
Query: 145 KHSRNLWGPVDGLGAYTPPSLSNIHALGA 59
K SR+L DGL + PS +NIH+L +
Sbjct: 4 KSSRSLILYGDGLARFVDPSNTNIHSLAS 32
>At5g07280.1 68418.m00830 leucine-rich repeat protein kinase,
putative / extra sporogenous cells (ESP) identical to
extra sporogenous cells [Arabidopsis thaliana]
gi|23304947|emb|CAD42912; contains Pfam domains PF00560:
Leucine Rich Repeat and PF00069: Protein kinase domain
Length = 1192
Score = 26.2 bits (55), Expect = 8.0
Identities = 17/57 (29%), Positives = 24/57 (42%), Gaps = 1/57 (1%)
Frame = -2
Query: 196 VSGQSILQTIPQENHRGKHSRNLWGPVDGLGAYTPPSLSNIHALGAFKRFKCFL-GP 29
VS S+ IP E + + NL+ ++ P + NI L F CF GP
Sbjct: 169 VSNNSLSGEIPPEIGKLSNLSNLYMGLNSFSGQIPSEIGNISLLKNFAAPSCFFNGP 225
>At3g53540.1 68416.m05912 expressed protein
Length = 924
Score = 26.2 bits (55), Expect = 8.0
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = -2
Query: 253 SPNFPINLYEALFHNFQDFVSGQSILQTIPQENHRGKHSR 134
SP F EAL N QDF +G + + QE + K S+
Sbjct: 40 SPEFNSCHCEALSENKQDFPTGVPMKSLLAQEMSKQKESK 79
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,907,803
Number of Sequences: 28952
Number of extensions: 179693
Number of successful extensions: 470
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 457
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 575830496
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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