Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0666
(423 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 100 1e-22
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 2e-09
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 54 9e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 48 5e-07
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 47 1e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 41 9e-05
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 32 0.032
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 32 0.032
SPCC1183.11 ||SPCC31H12.01|MS ion channel protein 1|Schizosaccha... 30 0.13
SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor, ... 27 1.6
SPBC106.17c |cys2||O-acetyltransferase |Schizosaccharomyces pomb... 26 2.1
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 3.7
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 25 6.4
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 25 6.4
SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces pomb... 24 8.5
SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor Mc... 24 8.5
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 24 8.5
SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein Usp104|Schi... 24 8.5
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 100 bits (239), Expect = 1e-22
Identities = 44/56 (78%), Positives = 51/56 (91%)
Frame = +1
Query: 256 DGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
DGNGTIDF EFLTMMARKMKDTD+EEE+REAF+VFDKDGN +I+ EL HV+T+LG
Sbjct: 60 DGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLG 115
Score = 92.3 bits (219), Expect = 3e-20
Identities = 43/53 (81%), Positives = 48/53 (90%)
Frame = +2
Query: 95 LTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 253
LT+EQIAEF+EAFSLFD+D DG IT+ ELG VMRSLGQ+PT AELQDMINEVD
Sbjct: 6 LTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVD 58
Score = 53.6 bits (123), Expect = 1e-08
Identities = 27/57 (47%), Positives = 38/57 (66%)
Frame = +2
Query: 83 MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 253
M D EE++ +EAF +FDKDG+G IT +EL V+ SLG+ ++ E+ DMI E D
Sbjct: 78 MKDTDNEEEV---REAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREAD 131
Score = 32.7 bits (71), Expect = 0.024
Identities = 18/55 (32%), Positives = 30/55 (54%)
Frame = +1
Query: 253 RDGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTN 417
+DGNG I E LT + + + S+EE+ + R D DG+ I+ E V+++
Sbjct: 96 KDGNGYITVEE-LTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
Score = 32.3 bits (70), Expect = 0.032
Identities = 18/43 (41%), Positives = 26/43 (60%)
Frame = +1
Query: 295 MMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
M R + D + E REAF +FD+D + I++ EL VM +LG
Sbjct: 1 MTTRNLTD-EQIAEFREAFSLFDRDQDGNITSNELGVVMRSLG 42
Score = 27.1 bits (57), Expect = 1.2
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +2
Query: 113 AEFKEAFSLFDKDGDGTITTKELGTVM-RSLGQNPTEAELQDMINEVD 253
AE ++ + D DG+GTI E T+M R + E E+++ D
Sbjct: 48 AELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFD 95
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 56.0 bits (129), Expect = 2e-09
Identities = 27/51 (52%), Positives = 36/51 (70%)
Frame = +2
Query: 98 TEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 250
++EQ E KEAF L+D D DG I T +G+V+RSLG N T+AEL + NE+
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL 54
Score = 51.2 bits (117), Expect = 6e-08
Identities = 22/51 (43%), Positives = 35/51 (68%)
Frame = +1
Query: 271 IDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
ID +F++ ++ K+++T+SEEE +AFRVFDKD + +I A+ M LG
Sbjct: 58 IDEKKFMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLG 108
Score = 44.8 bits (101), Expect = 6e-06
Identities = 23/64 (35%), Positives = 39/64 (60%), Gaps = 1/64 (1%)
Frame = +2
Query: 77 STMADQLTE-EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 253
S ++++L E E E+ +AF +FDKD G I T + M++LG+ ++ E+Q M+ E D
Sbjct: 65 SFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEAD 124
Query: 254 GTET 265
T +
Sbjct: 125 PTNS 128
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 54.0 bits (124), Expect = 9e-09
Identities = 24/54 (44%), Positives = 35/54 (64%)
Frame = +2
Query: 92 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVD 253
QLT QI E KEAF+L DKDGDG I +++ T++ SL Q+ +E + M ++
Sbjct: 41 QLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESIN 94
Score = 29.9 bits (64), Expect = 0.17
Identities = 11/30 (36%), Positives = 22/30 (73%)
Frame = +1
Query: 331 EEIREAFRVFDKDGNXFISAAELRHVMTNL 420
+E++EAF + DKDG+ I +++ ++T+L
Sbjct: 48 QELKEAFALLDKDGDGNIGREDVKTMLTSL 77
Score = 27.9 bits (59), Expect = 0.69
Identities = 14/54 (25%), Positives = 25/54 (46%)
Frame = +1
Query: 262 NGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
N I+ FLT M + ++ EAF FD + I + +R ++++G
Sbjct: 94 NPPINLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKIPISTMRDALSSMG 147
Score = 24.2 bits (50), Expect = 8.5
Identities = 11/39 (28%), Positives = 18/39 (46%)
Frame = +2
Query: 125 EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMI 241
EAFS FD G I + + S+G E++ ++
Sbjct: 121 EAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESIL 159
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 48.4 bits (110), Expect = 5e-07
Identities = 23/56 (41%), Positives = 33/56 (58%)
Frame = +2
Query: 92 QLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDGT 259
++TEEQ + EAF LFD D D I EL MR+LG N ++E+ ++ + D T
Sbjct: 30 EITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKT 85
Score = 41.1 bits (92), Expect = 7e-05
Identities = 20/56 (35%), Positives = 29/56 (51%)
Frame = +1
Query: 253 RDGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTNL 420
+ G G + +F+ +M K+ + D EEI+ AF +FD D IS LR V L
Sbjct: 84 KTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKEL 139
Score = 30.7 bits (66), Expect = 0.098
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +1
Query: 322 DSEEEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
+ ++I EAF++FD D + I ELR M LG
Sbjct: 34 EQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Score = 27.1 bits (57), Expect = 1.2
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +1
Query: 256 DGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMT 414
D + ID+ E M R + + E+ + R FDK G ++ + VMT
Sbjct: 49 DKDNAIDYHELRAAM-RALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMT 100
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 46.8 bits (106), Expect = 1e-06
Identities = 19/40 (47%), Positives = 29/40 (72%)
Frame = +2
Query: 119 FKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDM 238
+K+AFSLFD+ G G I +G ++R+ GQNPT AE+ ++
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEI 47
Score = 39.1 bits (87), Expect = 3e-04
Identities = 21/53 (39%), Positives = 31/53 (58%), Gaps = 2/53 (3%)
Frame = +1
Query: 271 IDFPEFLTMMARKMK-DTDSE-EEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
+D +FL ++ R D + EE + F+VFDKD I ELR+V+T+LG
Sbjct: 55 VDMEQFLQVLNRPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLG 107
Score = 37.1 bits (82), Expect = 0.001
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +2
Query: 116 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEV 250
EF + F +FDKD G I EL V+ SLG+ + E+ +++ V
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV 122
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 40.7 bits (91), Expect = 9e-05
Identities = 18/52 (34%), Positives = 32/52 (61%)
Frame = +1
Query: 256 DGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVM 411
DG G +DF EF+ ++ + EE+++ AF+++D D + +IS EL V+
Sbjct: 68 DGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVL 119
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 32.3 bits (70), Expect = 0.032
Identities = 16/52 (30%), Positives = 30/52 (57%)
Frame = +1
Query: 256 DGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVM 411
D NG IDF EF+ ++ + + +++ AF+++D D N IS E+ ++
Sbjct: 75 DKNGYIDFKEFICALSVTSRG-ELNDKLIWAFQLYDLDNNGLISYDEMLRIV 125
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 32.3 bits (70), Expect = 0.032
Identities = 18/47 (38%), Positives = 26/47 (55%)
Frame = +2
Query: 104 EQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMIN 244
E E +EAF LFD G I ++L LG+N T+ +LQ M++
Sbjct: 9 EMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLD 55
Score = 27.5 bits (58), Expect = 0.91
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +1
Query: 298 MARKMKDTDSEEEIREAFRVFDKDGNXFISAAELRHVMTNLG 423
M+ K + +EE EAF +FD +I +LR LG
Sbjct: 1 MSVSTKRLEMDEEAEEAFDLFDVTHKGYIDFEDLRRSCAQLG 42
>SPCC1183.11 ||SPCC31H12.01|MS ion channel protein
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1011
Score = 30.3 bits (65), Expect = 0.13
Identities = 17/61 (27%), Positives = 33/61 (54%), Gaps = 5/61 (8%)
Frame = +2
Query: 89 DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQ-----NPTEAELQDMINEVD 253
D+ E A + +S+FD+D +G IT +E+ +G+ + + +L D I+++D
Sbjct: 538 DETGEVDNATLEACYSIFDRDLNGDITCEEIELACVEIGKERKSISASLRDLNDSISKLD 597
Query: 254 G 256
G
Sbjct: 598 G 598
>SPAC1327.01c ||SPAC1783.09c, SPAC18G6.16c|transcription factor,
zf-fungal binuclear cluster type |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 977
Score = 26.6 bits (56), Expect = 1.6
Identities = 9/27 (33%), Positives = 15/27 (55%)
Frame = -2
Query: 233 LEVRLLWGSVLATSSRCPALWS*WCHR 153
LE++ WGS+ A + +W+ W R
Sbjct: 850 LEMKNYWGSISALCDKMSEIWADWVQR 876
>SPBC106.17c |cys2||O-acetyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 504
Score = 26.2 bits (55), Expect = 2.1
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = -3
Query: 319 CPSSCAPSLSRTRESLSCRFRPVY 248
CP+S PS S T SLS PVY
Sbjct: 51 CPNSVDPSASITSPSLSSGPEPVY 74
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 25.4 bits (53), Expect = 3.7
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = +2
Query: 125 EAFSLFDKDGDGTITTKELGTVMR 196
+ F FD+D DG + +EL + R
Sbjct: 311 DLFYQFDRDNDGALNNEELSALFR 334
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 24.6 bits (51), Expect = 6.4
Identities = 10/33 (30%), Positives = 19/33 (57%)
Frame = +1
Query: 268 TIDFPEFLTMMARKMKDTDSEEEIREAFRVFDK 366
T DFPE L+ +++ EE+++ + + DK
Sbjct: 579 TYDFPESLSHCVKRLPTPIPAEELQKRYNLRDK 611
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 24.6 bits (51), Expect = 6.4
Identities = 10/37 (27%), Positives = 18/37 (48%)
Frame = -3
Query: 148 FVEQ*ECLLKLGDLFFRQLIRHCRLTWIIWSYTKKSQ 38
F E EC+ L ++ + C+ W I+S+ S+
Sbjct: 1113 FCEPLECVETLVQIYLENNVECCQCIWDIFSFLSDSE 1149
>SPAC31A2.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 24.2 bits (50), Expect = 8.5
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +2
Query: 131 FSLFDKDGDGTITTKELGTVMRSLGQNPTEAEL 229
F KD +TTK++ T+ ++ +PT+ +
Sbjct: 321 FERLRKDSSLQLTTKDINTLFSTIALSPTKLSM 353
>SPAPB1E7.02c |mcl1|slr3|DNA polymerase alpha accessory factor
Mcl1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 24.2 bits (50), Expect = 8.5
Identities = 20/57 (35%), Positives = 26/57 (45%)
Frame = +2
Query: 62 DNPS*STMADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQ 232
+NP ST L E Q+ L D GDG +T E ++ R L N +A LQ
Sbjct: 707 NNPDASTSVPVLEELQLRNKLFLTLLEDSIGDGDVTEDEKISIAR-LEANIDKALLQ 762
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 24.2 bits (50), Expect = 8.5
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -3
Query: 331 PRYPCPSSCAPSLSRTRESL 272
P P PSS APS+S R S+
Sbjct: 1237 PPVPAPSSEAPSVSTPRSSV 1256
>SPAC4D7.13 |usp104|prp40|U1 snRNP-associated protein
Usp104|Schizosaccharomyces pombe|chr 1|||Manual
Length = 695
Score = 24.2 bits (50), Expect = 8.5
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = +1
Query: 268 TIDFPEFLTMMARKMKDTDSEEEIRE 345
T + PE + ++ K+KD + E + E
Sbjct: 491 TSNIPEIIARLSEKLKDREESEAVTE 516
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,409,857
Number of Sequences: 5004
Number of extensions: 24074
Number of successful extensions: 93
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 93
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 150383836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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