BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0610 (485 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1687.13c |csn5||COP9/signalosome complex subunit Csn5|Schizo... 66 2e-12 SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulat... 42 7e-05 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 29 0.49 SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schiz... 27 1.1 SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pom... 26 2.6 SPBC2D10.07c |||mitochondrial inner membrane peptidase complex c... 26 3.5 SPAC17A2.01 |bsu1|SPAC1B1.05, bsu1|high-affinity import carrier ... 25 4.6 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 6.1 SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 25 6.1 SPAC11D3.07c |||transcription factor|Schizosaccharomyces pombe|c... 25 8.0 >SPAC1687.13c |csn5||COP9/signalosome complex subunit Csn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 299 Score = 66.5 bits (155), Expect = 2e-12 Identities = 33/72 (45%), Positives = 44/72 (61%) Frame = +3 Query: 198 PTFLQRHKDISSCIAEDGDHARSGGTLEVMGLLLGKVDANTMIVMDSFALPVEGTETRVN 377 P F + K + + + H G LEVMG + GKV+ ++I++DSFALPVEGTETRVN Sbjct: 29 PEFFRSVKISAVALLKMLRHVSQGMPLEVMGYVQGKVEGASLIILDSFALPVEGTETRVN 88 Query: 378 AQAQXYEYMTAY 413 A + EY Y Sbjct: 89 AHEEAQEYSVQY 100 Score = 50.4 bits (115), Expect = 1e-07 Identities = 17/44 (38%), Positives = 35/44 (79%) Frame = +1 Query: 121 SNVDDIYRYDKKQQQDILAAKPWEKDPHFFKDIKISALALLKMV 252 + +++++R+D+++++ + PW+ DP FF+ +KISA+ALLKM+ Sbjct: 3 NQLENVFRFDEEKERAKIRESPWKHDPEFFRSVKISAVALLKML 46 Score = 30.7 bits (66), Expect = 0.12 Identities = 10/11 (90%), Positives = 10/11 (90%) Frame = +2 Query: 449 ENAIGWYHSHP 481 EN IGWYHSHP Sbjct: 111 ENVIGWYHSHP 121 >SPAC31G5.13 |rpn11|pad1, sks1, bfr2, mts5|19S proteasome regulatory subunit Rpn11|Schizosaccharomyces pombe|chr 1|||Manual Length = 308 Score = 41.5 bits (93), Expect = 7e-05 Identities = 22/43 (51%), Positives = 28/43 (65%), Gaps = 1/43 (2%) Frame = +3 Query: 255 HARSGGTLEVMGLLLGK-VDANTMIVMDSFALPVEGTETRVNA 380 H R G +EVMGL+LG+ VD T+ V+D FA+P GT V A Sbjct: 43 HGRHGTPMEVMGLMLGEFVDDFTVRVVDVFAMPQSGTGVSVEA 85 Score = 29.5 bits (63), Expect = 0.28 Identities = 9/12 (75%), Positives = 10/12 (83%) Frame = +2 Query: 449 ENAIGWYHSHPG 484 E +GWYHSHPG Sbjct: 105 EMVVGWYHSHPG 116 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 28.7 bits (61), Expect = 0.49 Identities = 14/56 (25%), Positives = 29/56 (51%) Frame = +3 Query: 252 DHARSGGTLEVMGLLLGKVDANTMIVMDSFALPVEGTETRVNAQAQXYEYMTAYIE 419 + + G V+G+LLG+ + + + V +S+A+P E E + + +M + E Sbjct: 32 NRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWFLDHNFMESMNE 87 >SPBC15D4.07c |atg9|apg9|autophagy associated protein Atg9 |Schizosaccharomyces pombe|chr 2|||Manual Length = 702 Score = 27.5 bits (58), Expect = 1.1 Identities = 12/47 (25%), Positives = 22/47 (46%) Frame = -1 Query: 188 HGLAARISCCCFLSYRYISSTFETVSMLFAITQVF*AIDACASALVD 48 H A + C SYR ++ +E + +L +F +C+ +VD Sbjct: 588 HSKAVQQEFCSLYSYRIVNLLWEILGILLTPVLLFFTFPSCSQDIVD 634 >SPBC19F8.03c |||clathrin binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 26.2 bits (55), Expect = 2.6 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = -3 Query: 171 DILLLFLVVSIYIINVRNGFNVIRHHPSFLSNR 73 D+L+LF V+I +INV + + HH + S R Sbjct: 188 DLLVLFKAVNIGVINVLEHYFEMGHHDAAQSLR 220 >SPBC2D10.07c |||mitochondrial inner membrane peptidase complex catalytic subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 157 Score = 25.8 bits (54), Expect = 3.5 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +2 Query: 134 IYIDTTRNNNRISSPLNH 187 IY+D T +N +I+ PL H Sbjct: 90 IYVDPTSSNKKITIPLGH 107 >SPAC17A2.01 |bsu1|SPAC1B1.05, bsu1|high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1|Schizosaccharomyces pombe|chr 1|||Manual Length = 526 Score = 25.4 bits (53), Expect = 4.6 Identities = 10/30 (33%), Positives = 16/30 (53%) Frame = -3 Query: 186 WFSGEDILLLFLVVSIYIINVRNGFNVIRH 97 W I+ +F ++ IY+I NGF I + Sbjct: 77 WAKRWSIVFMFCLMQIYVIWTSNGFGSIEY 106 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.0 bits (52), Expect = 6.1 Identities = 14/42 (33%), Positives = 24/42 (57%) Frame = -2 Query: 364 SVPSTGSANESITIIVFASTLPSNSPMTSSVPPDLA*SPSSA 239 S PS+ S++ + + S++PS S +SS L+ S SS+ Sbjct: 220 SSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSS 261 >SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 892 Score = 25.0 bits (52), Expect = 6.1 Identities = 13/44 (29%), Positives = 24/44 (54%) Frame = +1 Query: 31 LSHTMASTSADAQASIAQKTWVMANNIETVSNVDDIYRYDKKQQ 162 ++ T+ TS + + +AQ T++ VSNV+ +RY + Q Sbjct: 212 ITGTVTRTS-EVRPELAQGTFICEECHTVVSNVEQAFRYTEPTQ 254 >SPAC11D3.07c |||transcription factor|Schizosaccharomyces pombe|chr 1|||Manual Length = 603 Score = 24.6 bits (51), Expect = 8.0 Identities = 16/61 (26%), Positives = 28/61 (45%), Gaps = 1/61 (1%) Frame = +3 Query: 195 RPTFLQRHKDISSCIA-EDGDHARSGGTLEVMGLLLGKVDANTMIVMDSFALPVEGTETR 371 R L+ D+ IA E + R +E+ L+ DA ++++ PV+ T TR Sbjct: 76 RLKLLEEKIDLLLDIATETSEFKRENKAIELPSLVTQIKDAESIVIKHRQGSPVDNTPTR 135 Query: 372 V 374 + Sbjct: 136 I 136 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,140,880 Number of Sequences: 5004 Number of extensions: 46616 Number of successful extensions: 134 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 121 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 133 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 188065158 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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