Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0596
(408 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 113 4e-26
At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 112 1e-25
At1g67280.1 68414.m07657 lactoylglutathione lyase, putative / gl... 29 0.91
At1g21740.1 68414.m02721 expressed protein contains Pfam domains... 29 0.91
At5g35330.2 68418.m04188 methyl-CpG-binding domain-containing pr... 29 1.2
At5g35330.1 68418.m04187 methyl-CpG-binding domain-containing pr... 29 1.2
At3g44670.1 68416.m04804 disease resistance protein RPP1-Ws[A,C]... 27 4.8
At5g54560.1 68418.m06792 hypothetical protein contains Pfam prof... 27 6.4
At5g54550.1 68418.m06791 expressed protein contains Pfam profile... 27 6.4
At4g24670.2 68417.m03532 alliinase family protein contains Pfam ... 27 6.4
At4g24670.1 68417.m03531 alliinase family protein contains Pfam ... 27 6.4
At4g31240.2 68417.m04435 expressed protein 26 8.5
At4g31240.1 68417.m04434 expressed protein 26 8.5
>At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal
protein S6, Arabidopsis thaliana, PID:g2662469
Length = 250
Score = 113 bits (272), Expect = 4e-26
Identities = 51/81 (62%), Positives = 60/81 (74%)
Frame = +3
Query: 27 MKLNVSYPATGCQKLFEVXDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVXGGNDKQG 206
MK NV+ P TGCQK E+ D+ KLR FY+KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFYDKRISQEVSGDALGEEFKGYVFKIKGGCDKQG 60
Query: 207 FPMKQGVLTNSRVRLLCQRAT 269
FPMKQGVLT RVRLL R T
Sbjct: 61 FPMKQGVLTPGRVRLLLHRGT 81
Score = 41.5 bits (93), Expect = 2e-04
Identities = 22/41 (53%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Frame = +2
Query: 260 KGHSCYRP--RRDGERKRKSVXGCIVDANLSVLALCYCAXG 376
+G C+R RR GER+RKSV GCIV +LSVL L G
Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKG 119
>At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)
Length = 249
Score = 112 bits (269), Expect = 1e-25
Identities = 50/81 (61%), Positives = 60/81 (74%)
Frame = +3
Query: 27 MKLNVSYPATGCQKLFEVXDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVXGGNDKQG 206
MK NV+ P TGCQK E+ D+ KLR F++KR+ EV D LG+E+KGYV ++ GG DKQG
Sbjct: 1 MKFNVANPTTGCQKKLEIDDDQKLRAFFDKRLSQEVSGDALGEEFKGYVFKIMGGCDKQG 60
Query: 207 FPMKQGVLTNSRVRLLCQRAT 269
FPMKQGVLT RVRLL R T
Sbjct: 61 FPMKQGVLTPGRVRLLLHRGT 81
Score = 41.5 bits (93), Expect = 2e-04
Identities = 22/41 (53%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Frame = +2
Query: 260 KGHSCYRP--RRDGERKRKSVXGCIVDANLSVLALCYCAXG 376
+G C+R RR GER+RKSV GCIV +LSVL L G
Sbjct: 79 RGTPCFRGHGRRTGERRRKSVRGCIVSPDLSVLNLVIVKKG 119
>At1g67280.1 68414.m07657 lactoylglutathione lyase, putative /
glyoxalase I, putative similar to putative
lactoylglutathione lyase SP:Q39366, GI:2494843 from
[Brassica oleracea]
Length = 350
Score = 29.5 bits (63), Expect = 0.91
Identities = 17/56 (30%), Positives = 26/56 (46%)
Frame = +3
Query: 48 PATGCQKLFEVXDEHKLRIFYEKRMGAEVEADQLGDEWKGYVLRVXGGNDKQGFPM 215
P CQ + V D + FYEK G E+ + E+K Y + + G + FP+
Sbjct: 216 PEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYK-YTIAMMGYGPEDKFPV 270
>At1g21740.1 68414.m02721 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 953
Score = 29.5 bits (63), Expect = 0.91
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = +3
Query: 63 QKLF-EVXDEHKLRIFYEKRMGAEVEADQLGDE 158
+KL+ EV DE KLR+ YE++ + D LG E
Sbjct: 602 KKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAE 634
>At5g35330.2 68418.m04188 methyl-CpG-binding domain-containing
protein similar to methyl-CpG binding protein MBD4 [Mus
musculus] GI:3800807; contains Pfam profile PF01429:
Methyl-CpG binding domain
Length = 272
Score = 29.1 bits (62), Expect = 1.2
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3
Query: 6 GTRLTAVMKLNVSYPATGCQKLFEVXDE 89
GTRL A+ K N+S P G Q+L + E
Sbjct: 114 GTRLWAIDKPNISRPPAGWQRLLRIRGE 141
>At5g35330.1 68418.m04187 methyl-CpG-binding domain-containing
protein similar to methyl-CpG binding protein MBD4 [Mus
musculus] GI:3800807; contains Pfam profile PF01429:
Methyl-CpG binding domain
Length = 272
Score = 29.1 bits (62), Expect = 1.2
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +3
Query: 6 GTRLTAVMKLNVSYPATGCQKLFEVXDE 89
GTRL A+ K N+S P G Q+L + E
Sbjct: 114 GTRLWAIDKPNISRPPAGWQRLLRIRGE 141
>At3g44670.1 68416.m04804 disease resistance protein
RPP1-Ws[A,C]-like (TIR-NBS-LRR class), putative domain
signature TIR-NBS-LRR exists, suggestive of a disease
resistance protein. Closest Col-0 homolog to both RPP1
Ws-A and RPP1 Ws-C
Length = 872
Score = 27.1 bits (57), Expect = 4.8
Identities = 14/39 (35%), Positives = 17/39 (43%)
Frame = +2
Query: 179 CRWRQRQARIPDETGRPDKQPXSSSVSKGHSCYRPRRDG 295
C QR+ R + PD P SS VS C R + G
Sbjct: 791 CGILQRETRSLRRSSSPDLSPESSRVSSYDHCLRGKNHG 829
>At5g54560.1 68418.m06792 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 360
Score = 26.6 bits (56), Expect = 6.4
Identities = 17/46 (36%), Positives = 22/46 (47%)
Frame = -1
Query: 222 PVSSGILACRCRXRHEVHSPSIHRLTDQPLLRRPCAFRKRYEACAR 85
P S G L R E H+P I RL + L P A R+ ++C R
Sbjct: 202 PGSGGHLIGSWDPRTEDHNPKIQRLRFRNLPELPRAKRELLDSCYR 247
>At5g54550.1 68418.m06791 expressed protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 359
Score = 26.6 bits (56), Expect = 6.4
Identities = 17/46 (36%), Positives = 22/46 (47%)
Frame = -1
Query: 222 PVSSGILACRCRXRHEVHSPSIHRLTDQPLLRRPCAFRKRYEACAR 85
P S G L R E H+P I RL + L P A R+ ++C R
Sbjct: 200 PGSGGHLIGSWDLRTEDHNPKIQRLRFRNLPELPRAKRELLDSCYR 245
>At4g24670.2 68417.m03532 alliinase family protein contains Pfam
profiles: PF04864 allinase C-terminal domain, PF04863
alliinase EGF-like domain
Length = 440
Score = 26.6 bits (56), Expect = 6.4
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -2
Query: 281 VCNMSGPLTQKTNXAVCQDALFHRESLLVVAAXDTKYIALP 159
V + G +T K+ DA++ R LL AA DTK ++P
Sbjct: 317 VSDSCGNVTGKSFFDHSYDAMYERWKLLKQAAKDTKRFSVP 357
>At4g24670.1 68417.m03531 alliinase family protein contains Pfam
profiles: PF04864 allinase C-terminal domain, PF04863
alliinase EGF-like domain
Length = 440
Score = 26.6 bits (56), Expect = 6.4
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = -2
Query: 281 VCNMSGPLTQKTNXAVCQDALFHRESLLVVAAXDTKYIALP 159
V + G +T K+ DA++ R LL AA DTK ++P
Sbjct: 317 VSDSCGNVTGKSFFDHSYDAMYERWKLLKQAAKDTKRFSVP 357
>At4g31240.2 68417.m04435 expressed protein
Length = 392
Score = 26.2 bits (55), Expect = 8.5
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = -2
Query: 221 LFHRESLLVVAAXDTKYIALPFIA*LISLYFGAH 120
L H VVA +K + + I LYFGAH
Sbjct: 180 LTHESRNYVVARNGSKVLVSKLVGKTIGLYFGAH 213
>At4g31240.1 68417.m04434 expressed protein
Length = 392
Score = 26.2 bits (55), Expect = 8.5
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = -2
Query: 221 LFHRESLLVVAAXDTKYIALPFIA*LISLYFGAH 120
L H VVA +K + + I LYFGAH
Sbjct: 180 LTHESRNYVVARNGSKVLVSKLVGKTIGLYFGAH 213
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,246,786
Number of Sequences: 28952
Number of extensions: 153258
Number of successful extensions: 423
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 423
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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