Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0579
(490 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 105 4e-24
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 70 2e-13
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 57 2e-09
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 56 3e-09
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 54 1e-08
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 49 3e-07
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 46 4e-06
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 45 7e-06
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 43 2e-05
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 43 3e-05
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 37 0.001
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 37 0.001
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 32 0.053
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 0.87
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 28 0.87
SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ... 26 2.7
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 25 4.6
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 8.1
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 25 8.1
SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2... 25 8.1
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 105 bits (252), Expect = 4e-24
Identities = 48/79 (60%), Positives = 58/79 (73%)
Frame = +1
Query: 253 MNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLS 432
++LM W+ IPGK T WEGGLYKL M F ++ P+ P KC+F PPL HPNVYPSGTV LS
Sbjct: 36 LDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLS 95
Query: 433 LL*LKKXXGXPAITIKQIL 489
+L ++ PAITIKQIL
Sbjct: 96 IL-NEEEGWKPAITIKQIL 113
Score = 53.6 bits (123), Expect = 2e-08
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = +2
Query: 149 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDG 250
MS + RL EERK WR+DHPFGF A+P K+ DG
Sbjct: 1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDG 34
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 70.1 bits (164), Expect = 2e-13
Identities = 30/61 (49%), Positives = 36/61 (59%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
+ TW+C I G GTP+EGGLY + F D P P KFE HPNVY GTV +S+
Sbjct: 34 DFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKFECEFFHPNVYKDGTVCISI 93
Query: 436 L 438
L
Sbjct: 94 L 94
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 56.8 bits (131), Expect = 2e-09
Identities = 24/61 (39%), Positives = 35/61 (57%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
++ WE I G + T +EGG + + F D P P K KF + HPNV+P+G V +S+
Sbjct: 34 SIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISI 93
Query: 436 L 438
L
Sbjct: 94 L 94
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 56.0 bits (129), Expect = 3e-09
Identities = 24/61 (39%), Positives = 34/61 (55%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
N+M W I G TP+E G +KL + F + P+ P KF + HPNVY +G + L +
Sbjct: 32 NVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDI 91
Query: 436 L 438
L
Sbjct: 92 L 92
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 54.0 bits (124), Expect = 1e-08
Identities = 25/78 (32%), Positives = 42/78 (53%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
+L W+ I G +P+ GG++ L + F D P P K F + HPN+ +G++ L +
Sbjct: 29 DLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINSNGSICLDI 88
Query: 436 L*LKKXXGXPAITIKQIL 489
L + PA+TI ++L
Sbjct: 89 L---RDQWSPALTISKVL 103
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 49.2 bits (112), Expect = 3e-07
Identities = 24/61 (39%), Positives = 32/61 (52%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
NL+ W I G T +EG +K+ M F + P SP F P+ HPNV SG + L +
Sbjct: 57 NLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGNICLDI 116
Query: 436 L 438
L
Sbjct: 117 L 117
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 45.6 bits (103), Expect = 4e-06
Identities = 24/78 (30%), Positives = 39/78 (50%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
NL ++ + G + + +EGG + L + D+ P P +F + HPNV G + LS
Sbjct: 30 NLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHPNVDKLGRICLST 89
Query: 436 L*LKKXXGXPAITIKQIL 489
L K PA+ I+ +L
Sbjct: 90 L---KKDWSPALQIRTVL 104
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 44.8 bits (101), Expect = 7e-06
Identities = 23/78 (29%), Positives = 36/78 (46%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSL 435
NL W C G + + GG + + F D P P +F + HPN G V L++
Sbjct: 35 NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAI 94
Query: 436 L*LKKXXGXPAITIKQIL 489
LK+ P+I ++ +L
Sbjct: 95 --LKQQVFKPSIKLRSVL 110
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 43.2 bits (97), Expect = 2e-05
Identities = 24/69 (34%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = +1
Query: 286 GKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNV-YPSGTVXLSLL*LKKXXGX 462
G +GTP+EGG + + + D P P K F+ + HPNV +G + L +L K
Sbjct: 42 GPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDIL---KDQWS 98
Query: 463 PAITIKQIL 489
P T+K L
Sbjct: 99 PVYTMKSAL 107
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 42.7 bits (96), Expect = 3e-05
Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
Frame = +1
Query: 253 MNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNV-YPSGTVXL 429
+++ W+ I G TP+EGG + L + + P SP F+ + HPN+ + +G V +
Sbjct: 32 VDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNGEVCM 91
Query: 430 SLL 438
+L
Sbjct: 92 DIL 94
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 37.1 bits (82), Expect = 0.001
Identities = 19/62 (30%), Positives = 31/62 (50%)
Frame = +1
Query: 304 WEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYPSGTVXLSLL*LKKXXGXPAITIKQ 483
++GG +K R+ D+ P P K K + HPN+ G V L++L + P + +
Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL---RQDWNPVLNLNS 121
Query: 484 IL 489
IL
Sbjct: 122 IL 123
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 37.1 bits (82), Expect = 0.001
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPP 387
N++ W I G TP+EGG Y +IF D P P + P
Sbjct: 33 NILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP 76
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 31.9 bits (69), Expect = 0.053
Identities = 16/62 (25%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = +1
Query: 256 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDXPSSPXKCKFEPPLXHPNVYP-SGTVXLS 432
N+ + G TP+ GG++K+ + + P F + HPN+ SG+V L
Sbjct: 28 NMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLD 87
Query: 433 LL 438
++
Sbjct: 88 VI 89
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 0.87
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = -2
Query: 207 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 100
W LR+ +S++ + ++F K NSIY +F++ L
Sbjct: 71 WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 27.9 bits (59), Expect = 0.87
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -3
Query: 377 NLHFXGLDGXSSLKIIRSLYSPPSHGVPFLPGIAHSQV 264
N F G+ L + S SP HG P +PG+ H+ +
Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASL 479
>SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 527
Score = 26.2 bits (55), Expect = 2.7
Identities = 17/56 (30%), Positives = 28/56 (50%)
Frame = -1
Query: 337 RSYVVYTAHLPMESPFYQVSHIPKSLNS*TIWILHRPRDKTKGMVLTPSFPFFS*T 170
RS + A LPME+ +SH ++++ + P +T + TP+F F S T
Sbjct: 374 RSAFSHVARLPMETVSQLLSHYTETISQ------YLPPSETSPIPKTPNFKFISST 423
>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 25.4 bits (53), Expect = 4.6
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +1
Query: 265 TWECAIPGKKGTPWEGGLYK 324
TW C++ G+ G +G L+K
Sbjct: 142 TWNCSVYGRNGIKVDGKLFK 161
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 24.6 bits (51), Expect = 8.1
Identities = 19/90 (21%), Positives = 41/90 (45%)
Frame = -3
Query: 389 SGGSNLHFXGLDGXSSLKIIRSLYSPPSHGVPFLPGIAHSQVIKFMNHLDSS*ASRQNQR 210
SG +++ F L+ + L++ P +PF+ +AH+ ++ N + S + +
Sbjct: 81 SGRTSIRFNTLEDIKCSISLLVLFAEPDIDLPFVSQVAHNILVWLENLIPLS--NGMDDT 138
Query: 209 DGPYAKLSVLQLNVHLLSLTFSTKITRFMS 120
+ K+ +L++ L + S RF S
Sbjct: 139 PKSHLKVKILKVQETLSNSNDSWLWQRFSS 168
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 24.6 bits (51), Expect = 8.1
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -2
Query: 252 EPSGFFIGLATKPKGWSLRQAFRSSAKRALA 160
E SGF + L ++ + WSL + S LA
Sbjct: 773 EDSGFSLKLCSEVQEWSLNKVLNGSINEKLA 803
>SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 825
Score = 24.6 bits (51), Expect = 8.1
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2
Query: 287 VKRGLHGKVGCINYV*SS 340
++ G HGK G +NYV SS
Sbjct: 537 LETGWHGKFGSLNYVTSS 554
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,869,806
Number of Sequences: 5004
Number of extensions: 37460
Number of successful extensions: 101
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 190087364
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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