Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0559
(476 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65980.1 68414.m07486 peroxiredoxin type 2, putative strong s... 97 7e-21
At1g65970.1 68414.m07485 peroxiredoxin type 2, putative strong s... 96 9e-21
At1g60740.1 68414.m06838 peroxiredoxin type 2, putative strong s... 96 9e-21
At3g52960.1 68416.m05838 peroxiredoxin type 2, putative similar ... 95 2e-20
At1g65990.1 68414.m07488 type 2 peroxiredoxin-related / thiol sp... 83 7e-17
At3g06050.1 68416.m00692 alkyl hydroperoxide reductase/thiol spe... 73 1e-13
At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearl... 32 0.17
At3g26060.1 68416.m03245 peroxiredoxin Q, putative similar to pe... 32 0.23
At1g48130.1 68414.m05371 peroxiredoxin (PER1) / rehydrin, putati... 32 0.23
At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing ... 29 1.2
At5g52440.1 68418.m06507 HCF106 protein identical to HCF106 [Ara... 29 2.2
At3g02890.1 68416.m00284 PHD finger protein-related contains low... 29 2.2
At4g38650.1 68417.m05471 glycosyl hydrolase family 10 protein si... 27 6.6
At1g58440.1 68414.m06648 squalene monooxygenase, putative / squa... 27 6.6
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 8.7
>At1g65980.1 68414.m07486 peroxiredoxin type 2, putative strong
similarity to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profile: PF00578
AhpC/TSA (alkyl hydroperoxide reductase and
thiol-specific antioxidant) family
Length = 162
Score = 96.7 bits (230), Expect = 7e-21
Identities = 44/85 (51%), Positives = 58/85 (68%), Gaps = 3/85 (3%)
Frame = +2
Query: 11 IKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNA 181
I VGD +P + E+ ++ L AGKKV+LF VPGAFTP CS H+PG+++ A
Sbjct: 4 IAVGDVVPDGTISFFDENDQLQTASVHSLAAGKKVILFGVPGAFTPTCSMKHVPGFIEKA 63
Query: 182 DKLKSDGVAEIVCVSVNDPYVMAAW 256
++LKS GV EI+C SVNDP+VM AW
Sbjct: 64 EELKSKGVDEIICFSVNDPFVMKAW 88
Score = 38.3 bits (85), Expect = 0.003
Identities = 25/62 (40%), Positives = 35/62 (56%), Gaps = 1/62 (1%)
Frame = +1
Query: 283 VRMLADPSGNFIKALDLGTNLPXLG-GFRSKRFSMVIVDSKVQDLNVEPDGTGLSXSLAD 459
V+ +AD SG + L L +L G G RS+RF++++ D KV NVE G + S AD
Sbjct: 98 VKFVADGSGEYTHLLGLELDLKDKGLGVRSRRFALLLDDLKVTVANVESGGE-FTVSSAD 156
Query: 460 KI 465
I
Sbjct: 157 DI 158
>At1g65970.1 68414.m07485 peroxiredoxin type 2, putative strong
similarity to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profile: PF00578
AhpC/TSA (alkyl hydroperoxide reductase and
thiol-specific antioxidant) family
Length = 162
Score = 96.3 bits (229), Expect = 9e-21
Identities = 43/85 (50%), Positives = 59/85 (69%), Gaps = 3/85 (3%)
Frame = +2
Query: 11 IKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNA 181
I VGD +P + E+ V++ + AGKKV+LF VPGAFTP CS +H+PG++ A
Sbjct: 4 ITVGDVVPDGTISFFDENDQLQTVSVHSIAAGKKVILFGVPGAFTPTCSMSHVPGFIGKA 63
Query: 182 DKLKSDGVAEIVCVSVNDPYVMAAW 256
++LKS G+ EI+C SVNDP+VM AW
Sbjct: 64 EELKSKGIDEIICFSVNDPFVMKAW 88
Score = 37.1 bits (82), Expect = 0.006
Identities = 23/63 (36%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
Frame = +1
Query: 283 VRMLADPSGNFIKALDLGTNLPXLG-GFRSKRFSMVIVDSKVQDLNVEPDGTGLSXSLAD 459
V+ +AD SG + L L +L G G RS+RF++++ + KV NVE G S D
Sbjct: 98 VKFVADGSGEYTHLLGLELDLKDKGLGIRSRRFALLLDNLKVTVANVESGGEFTVSSAED 157
Query: 460 KIK 468
+K
Sbjct: 158 ILK 160
>At1g60740.1 68414.m06838 peroxiredoxin type 2, putative strong
similarity to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profile: PF00578
AhpC/TSA (alkyl hydroperoxide reductase and
thiol-specific antioxidant) family
Length = 162
Score = 96.3 bits (229), Expect = 9e-21
Identities = 43/85 (50%), Positives = 59/85 (69%), Gaps = 3/85 (3%)
Frame = +2
Query: 11 IKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNA 181
I VGD +P + E+ V++ + AGKKV+LF VPGAFTP CS +H+PG++ A
Sbjct: 4 ITVGDVVPDGTISFFDENDQLQTVSVHSIAAGKKVILFGVPGAFTPTCSMSHVPGFIGKA 63
Query: 182 DKLKSDGVAEIVCVSVNDPYVMAAW 256
++LKS G+ EI+C SVNDP+VM AW
Sbjct: 64 EELKSKGIDEIICFSVNDPFVMKAW 88
Score = 37.5 bits (83), Expect = 0.005
Identities = 24/72 (33%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Frame = +1
Query: 256 GAQHNTKGKVRMLADPSGNFIKALDLGTNLPXLG-GFRSKRFSMVIVDSKVQDLNVEPDG 432
G + V+ +AD SG + L L +L G G RS+RF++++ + KV NVE G
Sbjct: 89 GKTYQENKHVKFVADGSGEYTHLLGLELDLKDKGLGIRSRRFALLLDNLKVTVANVENGG 148
Query: 433 TGLSXSLADKIK 468
S D +K
Sbjct: 149 EFTVSSAEDILK 160
>At3g52960.1 68416.m05838 peroxiredoxin type 2, putative similar to
type 2 peroxiredoxin [Brassica rapa subsp. pekinensis]
GI:4928472; contains Pfam profile: PF00578 AhpC/TSA
(alkyl hydroperoxide reductase and thiol-specific
antioxidant) family
Length = 234
Score = 95.1 bits (226), Expect = 2e-20
Identities = 45/86 (52%), Positives = 58/86 (67%), Gaps = 4/86 (4%)
Frame = +2
Query: 11 IKVGDQLPAADLFEDSPAN----KVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQN 178
I VGD+LP + L P+ V + LTAGKK +LFAVPGAFTP CS+ H+PG+V
Sbjct: 73 ISVGDKLPDSTLSYLDPSTGDVKTVTVSSLTAGKKTILFAVPGAFTPTCSQKHVPGFVSK 132
Query: 179 ADKLKSDGVAEIVCVSVNDPYVMAAW 256
A +L+S G+ I C+SVND +VM AW
Sbjct: 133 AGELRSKGIDVIACISVNDAFVMEAW 158
>At1g65990.1 68414.m07488 type 2 peroxiredoxin-related / thiol
specific antioxidant / mal allergen family protein
similar to type 2 peroxiredoxin [Brassica rapa subsp.
pekinensis] GI:4928472; contains Pfam profiles PF00646:
F-box domain, PF00578: AhpC/TSA family
Length = 553
Score = 83.4 bits (197), Expect = 7e-17
Identities = 38/84 (45%), Positives = 57/84 (67%), Gaps = 3/84 (3%)
Frame = +2
Query: 11 IKVGDQLPAADLF---EDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNA 181
I VGD +P + +D V++ L AGKKV+LF VPGAF P CS H+ G+++ A
Sbjct: 4 IDVGDFVPDGSISFFDDDDQLQTVSVHSLAAGKKVILFGVPGAFPPTCSMNHVNGFIEKA 63
Query: 182 DKLKSDGVAEIVCVSVNDPYVMAA 253
++LKS+GV EI+C+S +DP+++ A
Sbjct: 64 EELKSNGVDEIICLSGDDPFMITA 87
>At3g06050.1 68416.m00692 alkyl hydroperoxide reductase/thiol
specific antioxidant (AhpC/TSA)/mal allergen family
protein identical to SP|Q9M7T0 Putative peroxiredoxin,
mitochondrial precursor {Arabidopsis thaliana}; similar
to thioredoxin peroxidase [Capsicum annuum] GI:18654477;
contains Pfam profile: PF00578 AhpC/TSA (alkyl
hydroperoxide reductase and thiol-specific antioxidant)
family
Length = 201
Score = 72.9 bits (171), Expect = 1e-13
Identities = 27/60 (45%), Positives = 42/60 (70%)
Frame = +2
Query: 77 ICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNADKLKSDGVAEIVCVSVNDPYVMAAW 256
+ ++ GKKVV+F +PGA+T CS+ H+P Y + DK K+ G+ ++CVSVNDP+ + W
Sbjct: 67 LSDIFKGKKVVIFGLPGAYTGVCSQQHVPSYKSHIDKFKAKGIDSVICVSVNDPFAINGW 126
>At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearly
identical to acyl-activating enzyme 18 [Arabidopsis
thaliana] GI:29893268; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501l; identical to cDNA
acyl-activating enzyme 18 (At1g55320) GI: 29893267
Length = 725
Score = 32.3 bits (70), Expect = 0.17
Identities = 25/78 (32%), Positives = 40/78 (51%), Gaps = 1/78 (1%)
Frame = +2
Query: 47 FEDSPANKVNICELTAGKKVVLFAVPGAFTPGCS-KTHLPGYVQNADKLKSDGVAEIVCV 223
F+DSP N++ I EL +V A+ G+F G + +P V + +A + V
Sbjct: 199 FDDSPVNRMTIKELREQVMLVANAISGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVV 258
Query: 224 SVNDPYVMAAWELSTTLK 277
S+ D + AA E++T LK
Sbjct: 259 SIADSF--AAKEIATRLK 274
>At3g26060.1 68416.m03245 peroxiredoxin Q, putative similar to
peroxiredoxin Q [Sedum lineare] GI:6899842; contains
Pfam profile: PF00578 AhpC/TSA (alkyl hydroperoxide
reductase and thiol-specific antioxidant) family
Length = 216
Score = 31.9 bits (69), Expect = 0.23
Identities = 18/47 (38%), Positives = 27/47 (57%)
Frame = +2
Query: 95 GKKVVLFAVPGAFTPGCSKTHLPGYVQNADKLKSDGVAEIVCVSVND 235
GK VVL+ P TPGC+K + + +K K G AE++ +S +D
Sbjct: 95 GKPVVLYFYPADETPGCTK-QACAFRDSYEKFKKAG-AEVIGISGDD 139
>At1g48130.1 68414.m05371 peroxiredoxin (PER1) / rehydrin, putative
identical to peroxiredoxin (Rehydrin homolog)
[Arabidopsis thaliana] SWISS-PROT:O04005; contains Pfam
profile: PF00578 AhpC/TSA (alkyl hydroperoxide reductase
and thiol-specific antioxidant) family
Length = 216
Score = 31.9 bits (69), Expect = 0.23
Identities = 23/82 (28%), Positives = 41/82 (50%)
Frame = +2
Query: 11 IKVGDQLPAADLFEDSPANKVNICELTAGKKVVLFAVPGAFTPGCSKTHLPGYVQNADKL 190
I +GD +P ++ ++ +K + + A VLF+ PG FTP C+ T L + A +
Sbjct: 4 ITLGDTVPNLEV--ETTHDKFKLHDYFANSWTVLFSHPGDFTPVCT-TELGAMAKYAHEF 60
Query: 191 KSDGVAEIVCVSVNDPYVMAAW 256
GV +++ +S +D W
Sbjct: 61 DKRGV-KLLGLSCDDVQSHKDW 81
>At5g46250.1 68418.m05693 RNA recognition motif (RRM)-containing
protein contains similarity to RNA-binding protein;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 422
Score = 29.5 bits (63), Expect = 1.2
Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%)
Frame = +3
Query: 279 KGAYASRSQRQLHQG-SGPGHQSAXARRFPLQKVLDGHR*QQGPRSECGAR 428
KG + +RQ HQG +G GH +A + P ++ + GPR G R
Sbjct: 350 KGRVVGQGRRQNHQGGNGIGHGTASSSSHPNYHPVEVSKRPPGPRMPDGTR 400
>At5g52440.1 68418.m06507 HCF106 protein identical to HCF106
[Arabidopsis thaliana] GI:4894914; contains Pfam profile
PF02416: mttA/Hcf106 family
Length = 260
Score = 28.7 bits (61), Expect = 2.2
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 302 PAATSSRLWTWAPICRRSEVSAPKGSRWSSLTARSK 409
P T S L+++ P+ R + S P S W SL S+
Sbjct: 15 PTITKSHLFSYPPLQSRYKASKPNLSSWFSLLGSSR 50
>At3g02890.1 68416.m00284 PHD finger protein-related contains low
similarity to PHD-finger domain proteins
Length = 963
Score = 28.7 bits (61), Expect = 2.2
Identities = 11/28 (39%), Positives = 20/28 (71%)
Frame = -3
Query: 186 LSAFCTYPGKCVLEHPGVKAPGTANNTT 103
L++ C++ G +L GV APG+++NT+
Sbjct: 48 LASQCSFNGDNLLRSSGVNAPGSSHNTS 75
>At4g38650.1 68417.m05471 glycosyl hydrolase family 10 protein
similar to tapetum specific protein GI:3885492 from [Zea
mays]
Length = 562
Score = 27.1 bits (57), Expect = 6.6
Identities = 18/53 (33%), Positives = 29/53 (54%)
Frame = +1
Query: 307 GNFIKALDLGTNLPXLGGFRSKRFSMVIVDSKVQDLNVEPDGTGLSXSLADKI 465
G+ I LG N+P F KRF + +++++ EPD L+ +LADK+
Sbjct: 223 GSAISKTILG-NIPYQEWF-VKRFDATVFENELKWYATEPDQGKLNYTLADKM 273
>At1g58440.1 68414.m06648 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2) 6566341 dbj AB008021.1 AB008021
Length = 531
Score = 27.1 bits (57), Expect = 6.6
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -3
Query: 219 HTISATPSDFSLSAFCTYPGKCVLEHPGVKAPGTAN 112
H + A PS + + +C+++ PG K P AN
Sbjct: 257 HVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIAN 292
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 26.6 bits (56), Expect = 8.7
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +2
Query: 113 FAVPGAFTPGCSKTHLPGYVQNADKLKSD 199
F VPG F GCS T+L Y Q ++K + D
Sbjct: 215 FLVPGNFPLGCSATYLTLY-QTSNKEEYD 242
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,678,982
Number of Sequences: 28952
Number of extensions: 233379
Number of successful extensions: 683
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 642
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 682
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 821630280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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