Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0556
(567 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g38560.1 68417.m05459 expressed protein 36 0.019
At2g41020.2 68415.m05066 WW domain-containing protein contains P... 34 0.076
At2g41020.1 68415.m05067 WW domain-containing protein contains P... 34 0.076
At2g16860.1 68415.m01939 GCIP-interacting family protein similar... 33 0.18
At2g16900.1 68415.m01946 expressed protein 32 0.23
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 30 0.94
At4g35110.2 68417.m04989 expressed protein 29 1.6
At4g35110.1 68417.m04988 expressed protein 29 1.6
At2g20960.1 68415.m02479 expressed protein pEARLI 4 gene product... 29 1.6
At3g59770.1 68416.m06670 sacI homology domain-containing protein... 29 2.2
At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A... 29 2.2
At1g45231.2 68414.m05189 WW domain-containing protein Since this... 29 2.9
At2g04170.1 68415.m00402 meprin and TRAF homology domain-contain... 28 5.0
At2g02060.1 68415.m00141 calcium-dependent protein kinase-relate... 27 6.6
At1g76420.1 68414.m08883 no apical meristem (NAM) family protein... 27 6.6
At1g74630.1 68414.m08643 pentatricopeptide (PPR) repeat-containi... 27 6.6
At5g12100.1 68418.m01421 pentatricopeptide (PPR) repeat-containi... 27 8.7
At4g19000.1 68417.m02798 IWS1 C-terminus family protein contains... 27 8.7
At4g04610.1 68417.m00674 5'-adenylylsulfate reductase (APR1) / P... 27 8.7
At2g04190.1 68415.m00404 meprin and TRAF homology domain-contain... 27 8.7
At1g06250.1 68414.m00661 lipase class 3 family protein similar t... 27 8.7
>At4g38560.1 68417.m05459 expressed protein
Length = 521
Score = 35.9 bits (79), Expect = 0.019
Identities = 14/31 (45%), Positives = 15/31 (48%)
Frame = +3
Query: 306 HRGCPNKSNIYHECSTFCIKRWKQGKLVPTE 398
H CPN N YHEC C KR G + E
Sbjct: 6 HHDCPNSGNPYHECHDQCFKRISSGDVPKKE 36
>At2g41020.2 68415.m05066 WW domain-containing protein contains Pfam
domain PF00397: WW domain
Length = 382
Score = 33.9 bits (74), Expect = 0.076
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +3
Query: 453 WEEVFDEGYGQHYFWNCAHNLGSWIPP 533
W E FDE G YF+N ++ W PP
Sbjct: 243 WIETFDEASGHKYFYNTRTHVSQWEPP 269
>At2g41020.1 68415.m05067 WW domain-containing protein contains Pfam
domain PF00397: WW domain
Length = 463
Score = 33.9 bits (74), Expect = 0.076
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +3
Query: 453 WEEVFDEGYGQHYFWNCAHNLGSWIPP 533
W E FDE G YF+N ++ W PP
Sbjct: 243 WIETFDEASGHKYFYNTRTHVSQWEPP 269
>At2g16860.1 68415.m01939 GCIP-interacting family protein similar to
GCIP-interacting protein mp29 (GI:27372623) [Mus
musculus]; similar to GCIP-interacting protein P29
(GI:11967379) [Homo sapiens]
Length = 298
Score = 32.7 bits (71), Expect = 0.18
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +3
Query: 306 HRGCPNKSNIYHECSTFCIKRWKQGK 383
H C N SN YHEC +C K+ + K
Sbjct: 8 HPDCINASNPYHECVEYCFKKIAEAK 33
>At2g16900.1 68415.m01946 expressed protein
Length = 382
Score = 32.3 bits (70), Expect = 0.23
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 300 KGHRGCPNKSNIYHECSTFCIKRWKQGK 383
K H C SN +HEC++ C+++ QG+
Sbjct: 6 KAHPDCRYSSNPFHECASDCLEKISQGR 33
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 30.3 bits (65), Expect = 0.94
Identities = 22/81 (27%), Positives = 37/81 (45%), Gaps = 2/81 (2%)
Frame = +2
Query: 47 NKISEHSKTTKEISTLGKIYNTAKCLY--XXXXXXXXXKEVF*NKMQIPPLSHLLVKSTK 220
++I HSK K++STL + N A Y ++ F +++ PPL + + ST
Sbjct: 5 SRIINHSKKLKDVSTLLRRENAATIRYYSNTNRAPLNREDTFNSRLGYPPLERISICSTS 64
Query: 221 K*LQKIMIVSLRKWKR*ALGR 283
I+ + R A+GR
Sbjct: 65 TLPVSIIFSTTRSNLSSAMGR 85
>At4g35110.2 68417.m04989 expressed protein
Length = 386
Score = 29.5 bits (63), Expect = 1.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 300 KGHRGCPNKSNIYHECSTFCIKRWKQG 380
K H C N +HEC++ C+++ QG
Sbjct: 6 KAHPDCVYADNPFHECASACLEKIAQG 32
>At4g35110.1 68417.m04988 expressed protein
Length = 386
Score = 29.5 bits (63), Expect = 1.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 300 KGHRGCPNKSNIYHECSTFCIKRWKQG 380
K H C N +HEC++ C+++ QG
Sbjct: 6 KAHPDCVYADNPFHECASACLEKIAQG 32
>At2g20960.1 68415.m02479 expressed protein pEARLI 4 gene product
[Arabidopsis thaliana] GI:871782
Length = 748
Score = 29.5 bits (63), Expect = 1.6
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +3
Query: 312 GCPNKSNIYHECSTFCIKR 368
GC N N +HEC+ C +R
Sbjct: 11 GCSNAGNPFHECTAICFER 29
>At3g59770.1 68416.m06670 sacI homology domain-containing protein /
WW domain-containing protein contains Pfam profiles
PF00397: WW domain, PF02383: SacI homology domain;
identical to cDNA SAC domain protein 9 (SAC9)
GI:31415734
Length = 1630
Score = 29.1 bits (62), Expect = 2.2
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +3
Query: 438 PLPPGWEEVFDEGYGQHYFWNCAHNLGSWIPP 533
PLPPGWE+ D G+ Y+ + +W P
Sbjct: 509 PLPPGWEKRADAVTGKSYYIDHNTKTTTWSHP 540
>At1g13320.1 68414.m01546 serine/threonine protein phosphatase 2A
(PP2A) 65 kDa regulatory subunit, putative similar to
protein phosphatase 2A 65 kDa regulatory subunit
GI:683502 from [Arabidopsis thaliana]
Length = 587
Score = 29.1 bits (62), Expect = 2.2
Identities = 15/50 (30%), Positives = 28/50 (56%)
Frame = -1
Query: 531 VVSTNPDYVHNSKNNVDHNLHQTLLPILVARAIALKLSPCVLNMSQSVPV 382
V++ + D V N K NV + Q+L+PI+ + + PC++ +S+ V
Sbjct: 519 VITASKDRVPNIKFNVA-KMMQSLIPIVDQAVVENMIRPCLVELSEDPDV 567
>At1g45231.2 68414.m05189 WW domain-containing protein Since this
genomic sequence region is unfinished, the annotated
gene may be missing a stop codon or start codon
Length = 538
Score = 28.7 bits (61), Expect = 2.9
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +3
Query: 453 WEEVFDEGYGQHYFWNCAHNLGSWIPP 533
W+ +D YG+ YF+N W PP
Sbjct: 167 WKVYWDSFYGRSYFYNFKTQESKWEPP 193
>At2g04170.1 68415.m00402 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to NtN2 [Medicago truncatula] GI:3776084; contains Pfam
profile PF00917: MATH domain
Length = 420
Score = 27.9 bits (59), Expect = 5.0
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +3
Query: 363 KRWKQGKLVPTETYLEHKEKVLE 431
K W GKL+P T+L+ E LE
Sbjct: 229 KEWGYGKLIPLTTFLDTNEGYLE 251
>At2g02060.1 68415.m00141 calcium-dependent protein kinase-related /
CDPK-related contains TIGRFAM TIGR01557: myb-like
DNA-binding domain, SHAQKYF class; contains Pfam
PF00249: Myb-like DNA-binding domain; similar to CDPK
substrate protein 1; CSP1 (GI:6942190) [Mesembryanthemum
crystallinum].
Length = 626
Score = 27.5 bits (58), Expect = 6.6
Identities = 14/38 (36%), Positives = 17/38 (44%)
Frame = -2
Query: 566 GRFRRHSTGMSGWYPRTQIMCTIPKIMLTITFIKHFFP 453
GR + T SG Y I K+ T TF+ H FP
Sbjct: 145 GRTKEQQTSESGGYDDDADFLHIKKMNDTTTFLSHHFP 182
>At1g76420.1 68414.m08883 no apical meristem (NAM) family protein
N-term similar to N-term of NAM GB:CAA63101 [Petunia x
hybrida] (apical meristem formation), CUC2 GB:BAA19529
[Arabidopsis thaliana], GRAB2 protein GB:CAA09372
[Triticum sp.]
Length = 334
Score = 27.5 bits (58), Expect = 6.6
Identities = 10/32 (31%), Positives = 17/32 (53%)
Frame = -1
Query: 540 NVRVVSTNPDYVHNSKNNVDHNLHQTLLPILV 445
NV ++ Y+HN + H+ H LP+L+
Sbjct: 177 NVGLIHNQISYLHNHSLSTTHHHHHEALPLLI 208
>At1g74630.1 68414.m08643 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 643
Score = 27.5 bits (58), Expect = 6.6
Identities = 24/73 (32%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
Frame = +1
Query: 232 EDYDSKPKKMEKMSLGKALSILSKAIEDALIKVIFIMNVLLFVLKDGSKVNWYRLRHI*N 411
E D K EK++L AL+ LSK ++K + I V+K SKV Y + +
Sbjct: 567 EKEDQVSKHSEKLALAFALARLSKGANIRIVKNLRICRDCHAVMKLTSKV--YGVEILVR 624
Query: 412 TRRKF*SY--GPC 444
R +F S+ G C
Sbjct: 625 DRNRFHSFKDGSC 637
>At5g12100.1 68418.m01421 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 816
Score = 27.1 bits (57), Expect = 8.7
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +1
Query: 274 LGKALSILSKAIEDALIKVIFIMNVLLFVLKDGSKVN 384
+GK L + ++ D + +FI NVL+ L G ++N
Sbjct: 195 VGKGLELFNRMKHDRIYPSVFIYNVLIDGLCKGKRMN 231
>At4g19000.1 68417.m02798 IWS1 C-terminus family protein contains
Pfam profile PF05909: IWS1 C-terminus
Length = 406
Score = 27.1 bits (57), Expect = 8.7
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +1
Query: 211 INEEIIAEDYDSKPKKMEKMSLGKALSILSKAIED 315
+N+ I D DS+ +++ K LGK + LSK+ E+
Sbjct: 228 LNDFRIDLDQDSRREQLIKSGLGKVIMFLSKSDEE 262
>At4g04610.1 68417.m00674 5'-adenylylsulfate reductase (APR1) / PAPS
reductase homolog (PRH19) identical to
5'-adenylylsulfate reductase [Arabidopsis thaliana]
GI:2738756; identical to cDNA PAPS reductase homolog
(PRH19) GI:1710111
Length = 465
Score = 27.1 bits (57), Expect = 8.7
Identities = 15/43 (34%), Positives = 24/43 (55%)
Frame = +1
Query: 184 STAVASVGQINEEIIAEDYDSKPKKMEKMSLGKALSILSKAIE 312
+T VA + + E + ED++ KK+E S L I+ KA+E
Sbjct: 75 ATMVAEIAEEVEVVEIEDFEELAKKLENAS---PLEIMDKALE 114
>At2g04190.1 68415.m00404 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 411
Score = 27.1 bits (57), Expect = 8.7
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +3
Query: 363 KRWKQGKLVPTETYLEHKEKVLE 431
K W GKL+P T+L+ + LE
Sbjct: 220 KEWGYGKLIPLSTFLDTSQGYLE 242
>At1g06250.1 68414.m00661 lipase class 3 family protein similar to
lipase GB:AAD01804 GI:4103627 from [Dianthus
caryophyllus]; contains Pfam profile PF01764: Lipase
Length = 423
Score = 27.1 bits (57), Expect = 8.7
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = -1
Query: 543 GNVRVVSTNPDYVHNSKNNVDHNLHQTLLPILV 445
G V V + D V+ KNN++ NL + +PI V
Sbjct: 225 GAVMSVLSAADLVYGKKNNININLQKKQVPITV 257
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,359,729
Number of Sequences: 28952
Number of extensions: 249130
Number of successful extensions: 692
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 674
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 692
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1092379416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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