BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0544 (520 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 136 1e-33 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 73 2e-14 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 73 3e-14 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 65 5e-12 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 46 4e-06 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 45 8e-06 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 37 0.002 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 31 0.14 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.2 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 2.9 SPBP16F5.08c |||flavin dependent monooxygenase |Schizosaccharomy... 26 3.9 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 3.9 SPAC7D4.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 6.8 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 9.0 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 9.0 SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|... 25 9.0 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 136 bits (330), Expect = 1e-33 Identities = 64/87 (73%), Positives = 68/87 (78%) Frame = +2 Query: 242 GTEGPYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 421 G + YVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE Sbjct: 48 GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107 Query: 422 AXLNPXANXREDDPXHVSKHSTTPALY 502 A LNP +N RE + + PA Y Sbjct: 108 APLNPKSN-REKMTQIIFETFNAPAFY 133 Score = 104 bits (250), Expect = 7e-24 Identities = 45/50 (90%), Positives = 49/50 (98%) Frame = +3 Query: 105 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKD 254 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKD Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKD 51 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 73.3 bits (172), Expect = 2e-14 Identities = 34/69 (49%), Positives = 47/69 (68%), Gaps = 1/69 (1%) Frame = +2 Query: 257 YVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAXLN 433 +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE Sbjct: 57 FVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEP--- 113 Query: 434 PXANXREDD 460 P AN R + Sbjct: 114 PLANIRNKE 122 Score = 58.8 bits (136), Expect = 4e-10 Identities = 25/44 (56%), Positives = 32/44 (72%) Frame = +3 Query: 123 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKD 254 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKD 55 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 72.5 bits (170), Expect = 3e-14 Identities = 27/62 (43%), Positives = 47/62 (75%) Frame = +2 Query: 260 VGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAXLNPX 439 VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE +NP Sbjct: 54 VGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPV 113 Query: 440 AN 445 AN Sbjct: 114 AN 115 Score = 48.0 bits (109), Expect = 8e-07 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +3 Query: 111 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 215 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 65.3 bits (152), Expect = 5e-12 Identities = 28/64 (43%), Positives = 38/64 (59%), Gaps = 1/64 (1%) Frame = +2 Query: 257 YVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAXLN 433 ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE LN Sbjct: 75 FIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLN 134 Query: 434 PXAN 445 P N Sbjct: 135 PPEN 138 Score = 40.7 bits (91), Expect = 1e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +3 Query: 123 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 206 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 45.6 bits (103), Expect = 4e-06 Identities = 19/51 (37%), Positives = 28/51 (54%) Frame = +2 Query: 293 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAXLNPXAN 445 + +K I +G V NWD +W + +L+ P EHP+L+TE NP N Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPEN 113 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +3 Query: 108 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 209 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 44.8 bits (101), Expect = 8e-06 Identities = 22/71 (30%), Positives = 39/71 (54%), Gaps = 1/71 (1%) Frame = +2 Query: 227 RDGRYGTEGPYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 403 RD + VG++ + G ++ + P E +++NWD ME++ +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 Query: 404 PVLLTEAXLNP 436 P+ +TE NP Sbjct: 117 PICMTEPLANP 127 Score = 35.9 bits (79), Expect = 0.004 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +3 Query: 123 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 218 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 36.7 bits (81), Expect = 0.002 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +3 Query: 108 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 209 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 Score = 35.9 bits (79), Expect = 0.004 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%) Frame = +2 Query: 257 YVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAXL 430 YV DE Q I + +K +GI+ +W+ W +L+V P E+ +++TE Sbjct: 52 YVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSW 111 Query: 431 NP 436 NP Sbjct: 112 NP 113 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 30.7 bits (66), Expect = 0.14 Identities = 19/66 (28%), Positives = 29/66 (43%) Frame = -1 Query: 340 IPVSDDSVFDGVFEGQDTSFALCLVSYVRSFCPIPTITP*WRGLPTIEGNTARGASSPAK 161 +P + +V +TSFAL S S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 160 PALHIP 143 P L+ P Sbjct: 123 PQLNSP 128 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.6 bits (56), Expect = 2.2 Identities = 15/45 (33%), Positives = 18/45 (40%) Frame = -1 Query: 238 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 104 PT+ P P N S P PL+TTN TS+S Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 2.9 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -1 Query: 244 PIPTITP*WRGLPTIEGNTARGASSPAKPAL 152 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPBP16F5.08c |||flavin dependent monooxygenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 447 Score = 25.8 bits (54), Expect = 3.9 Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 2/49 (4%) Frame = +2 Query: 353 IWHHT--FYNELRVAPEEHPVLLTEAXLNPXANXREDDPXHVSKHSTTP 493 +W++T N+L V P +P+L TE + P A P + + TP Sbjct: 46 VWNYTSTLSNKLPV-PSTNPILTTEPIVGPAALPVYPSPLYRDLQTNTP 93 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 25.8 bits (54), Expect = 3.9 Identities = 7/21 (33%), Positives = 13/21 (61%) Frame = +2 Query: 308 PIEHGIVTNWDDMEKIWHHTF 370 PI+ G V +W+ ++ W H + Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133 >SPAC7D4.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 551 Score = 25.0 bits (52), Expect = 6.8 Identities = 12/31 (38%), Positives = 18/31 (58%) Frame = +3 Query: 387 SPPRNTLSCSLRLPSTPXPTXEKMTXIMFRN 479 SPP++T S R PS P + EK + + +N Sbjct: 285 SPPQSTRPSSTRFPSVPL-SDEKNSIVSVKN 314 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 9.0 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +3 Query: 123 LVVDNGSGMCKAGFAG 170 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 24.6 bits (51), Expect = 9.0 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = +3 Query: 168 GDDAPRAVFPSIVGRPRHQGVMVGM 242 G+ PRA F ++ P H G+++ M Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM 526 >SPAC824.04 |||WD repeat protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 341 Score = 24.6 bits (51), Expect = 9.0 Identities = 11/41 (26%), Positives = 26/41 (63%), Gaps = 3/41 (7%) Frame = +2 Query: 323 IVTNWDDME-KIWH--HTFYNELRVAPEEHPVLLTEAXLNP 436 ++ + DD +W+ HTF+++ + P EH + ++++ +NP Sbjct: 271 VIGSADDRTLNVWNLRHTFHHKGKTRPPEHRI-VSQSIINP 310 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,121,867 Number of Sequences: 5004 Number of extensions: 45285 Number of successful extensions: 122 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 106 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 210309424 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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