Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0541
(589 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 73 3e-14
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 71 1e-13
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 70 2e-13
SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa... 49 6e-07
SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 32 0.071
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 29 0.67
SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual 29 0.67
SPBC215.14c |vps20||vacuolar sorting protein Vps20|Schizosacchar... 28 0.88
SPBC354.01 |gtp1|SPBC649.06|GTP binding protein Gtp1|Schizosacch... 28 0.88
SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity fac... 27 2.0
SPBC27.02c |ask1|mug181|DASH complex subunit Ask1|Schizosaccharo... 27 2.0
SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyce... 26 4.7
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 26 4.7
>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 537
Score = 72.9 bits (171), Expect = 3e-14
Identities = 35/73 (47%), Positives = 47/73 (64%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDSTVA 190
PN GKSSIIN+L + K CNV PG TK Q V L +I ++D PGIV S +SD+ +
Sbjct: 319 PNAGKSSIINTLRKKKVCNVAPIPGETKVWQYVALMKRIFLIDCPGIVPPSSNDSDAELL 378
Query: 191 LKNAIRVGSLKDP 229
LK +RV ++ +P
Sbjct: 379 LKGVVRVENVSNP 391
Score = 49.2 bits (112), Expect = 4e-07
Identities = 22/64 (34%), Positives = 39/64 (60%)
Frame = +1
Query: 253 TRANKQTLQDLYRIPDFSTPQEFFAQLAQRMGRFKKGGVPDQEAASRILLNDWNIGXVRY 432
+R + L+ Y I ++ EF A+LA++ GR KGG PD+ + ++++LND+ G + +
Sbjct: 400 SRCKVKHLERTYEISGWNDSTEFLAKLAKKGGRLLKGGEPDEASVAKMVLNDFMRGKIPW 459
Query: 433 FTEP 444
F P
Sbjct: 460 FIGP 463
>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 470
Score = 71.3 bits (167), Expect = 1e-13
Identities = 39/96 (40%), Positives = 58/96 (60%), Gaps = 8/96 (8%)
Frame = +2
Query: 11 PNVGKSSIINSL------NRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPE 172
PNVGKSS+IN+L RS C G+ G+T ++ V+LD+K++++DSPGIVF S
Sbjct: 278 PNVGKSSVINALVNRSANGRSAPCPAGNVAGMTTSLREVKLDNKLRLVDSPGIVFPSSDS 337
Query: 173 SDS--TVALKNAIRVGSLKDPVTPATAILRGLTSKP 274
D + + NA+ + DPV A+ IL+ L+ P
Sbjct: 338 KDDLYRLVMLNAVSSTKVDDPVAVASYILQFLSRVP 373
Score = 53.2 bits (122), Expect = 3e-08
Identities = 20/57 (35%), Positives = 34/57 (59%)
Frame = +1
Query: 274 LQDLYRIPDFSTPQEFFAQLAQRMGRFKKGGVPDQEAASRILLNDWNIGXVRYFTEP 444
L L D T +F +A++ GR +GG+P+ AA+ I++NDW+ G + ++ EP
Sbjct: 385 LPPLLNTSDIDTATDFLVNIARKRGRLGRGGIPNLNAAANIVINDWHAGRIEWWAEP 441
>SPAC3F10.16c |||GTP binding protein,
HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
Length = 616
Score = 70.1 bits (164), Expect = 2e-13
Identities = 33/77 (42%), Positives = 47/77 (61%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDSTVA 190
PNVGKSS IN+L SK +V STPG TK QT+ L K+ +LD PG+VF S + + +
Sbjct: 310 PNVGKSSTINALVGSKKVSVSSTPGKTKHFQTINLSEKVSLLDCPGLVFPSFATTQADLV 369
Query: 191 LKNAIRVGSLKDPVTPA 241
L + + L++ P+
Sbjct: 370 LDGVLPIDQLREYTGPS 386
Score = 26.6 bits (56), Expect = 2.7
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +1
Query: 364 GVPDQEAASRILLNDWNIGXVRYFTEP 444
G PD A+RILL D+ G + Y P
Sbjct: 440 GTPDDSRAARILLKDYVNGKLLYVHPP 466
>SPBC25B2.04c |||mitochondrial ribosome assembly
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 328
Score = 48.8 bits (111), Expect = 6e-07
Identities = 36/91 (39%), Positives = 54/91 (59%), Gaps = 10/91 (10%)
Frame = +2
Query: 11 PNVGKSSIINS-----LNRSKACNVGSTPGVTKQM-QTVQL--DSKIKILDSPGIVFQS- 163
PN GKSSI+NS L +SK+ VG+ PGVTK++ + V+L D + +LD+PGI+ S
Sbjct: 154 PNTGKSSILNSLRNVALRKSKSAIVGNYPGVTKRISEIVRLFNDMDVYMLDTPGIMTPSI 213
Query: 164 -GPESDSTVALKNAIRVGSLKDPVTPATAIL 253
PE ++L ++ G + PVT +L
Sbjct: 214 TKPEDMLKLSLVGCVKEG-IVHPVTVVDYLL 243
>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 31.9 bits (69), Expect = 0.071
Identities = 22/77 (28%), Positives = 40/77 (51%), Gaps = 3/77 (3%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKI---KILDSPGIVFQSGPESDS 181
PNVGKSS +N + R++ +V TK + D K +++D+PGI+ E +
Sbjct: 177 PNVGKSSFMNKVTRAQV-DVQPYAFTTKSLFVGHFDYKYLRWQVIDTPGIL-DHPLEQMN 234
Query: 182 TVALKNAIRVGSLKDPV 232
T+ +++ + L+ V
Sbjct: 235 TIEMQSITAMAHLRSAV 251
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 28.7 bits (61), Expect = 0.67
Identities = 28/95 (29%), Positives = 43/95 (45%), Gaps = 2/95 (2%)
Frame = +2
Query: 50 RSKACNVGSTPGVTKQMQTVQLDSKIKILD-SPGI-VFQSGPESDSTVALKNAIRVGSLK 223
R + N GS K ++ + S +K+ D SP VF+ G +S S VAL +R
Sbjct: 3245 REENDNEGSNVVEPKLLKIIADFSDVKVTDISPQTSVFELGLDSISAVALSGLLRKSGY- 3303
Query: 224 DPVTPATAILRGLTSKPYKICIEYQTSVHLRNSLR 328
D P + IL T + QT+ L +S++
Sbjct: 3304 DNANP-SLILTSSTISNLGFALNTQTNEELEDSIK 3337
>SPAC9.07c |||GTPase Rbg1 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 366
Score = 28.7 bits (61), Expect = 0.67
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +2
Query: 11 PNVGKSSIINSLN--RSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIV 154
P+VGKS+++ L RS+A T T +KI+ILD PGI+
Sbjct: 72 PSVGKSTLMTQLTGTRSEAAAYEFTTLTTVPGVLQYNGAKIQILDLPGII 121
>SPBC215.14c |vps20||vacuolar sorting protein
Vps20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 226
Score = 28.3 bits (60), Expect = 0.88
Identities = 14/32 (43%), Positives = 19/32 (59%)
Frame = +2
Query: 110 QLDSKIKILDSPGIVFQSGPESDSTVALKNAI 205
Q D K+K L+ PG +G + +V LKNAI
Sbjct: 162 QEDEKVKDLEKPGFTPSTGVDVLPSVPLKNAI 193
>SPBC354.01 |gtp1|SPBC649.06|GTP binding protein
Gtp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 28.3 bits (60), Expect = 0.88
Identities = 14/50 (28%), Positives = 32/50 (64%), Gaps = 2/50 (4%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVG-STPGVTKQMQTVQLD-SKIKILDSPGIV 154
P+VGKS++++++ ++K+ +T ++ D ++I++LD PGI+
Sbjct: 71 PSVGKSTLLSAITKTKSATASYEFTTLTAIPGVLEYDGAEIQMLDLPGII 120
>SPAC22G7.10 |||mRNA cleavage and polyadenylation specificity factor
complex subunit, Fip1 homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 344
Score = 27.1 bits (57), Expect = 2.0
Identities = 18/38 (47%), Positives = 21/38 (55%)
Frame = +1
Query: 259 ANKQTLQDLYRIPDFSTPQEFFAQLAQRMGRFKKGGVP 372
A + TL+D DFS PQ+F A LAQ G G VP
Sbjct: 156 AKQTTLRD-----DFS-PQKFMASLAQIPGPLPSGNVP 187
>SPBC27.02c |ask1|mug181|DASH complex subunit
Ask1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 307
Score = 27.1 bits (57), Expect = 2.0
Identities = 7/20 (35%), Positives = 16/20 (80%)
Frame = -2
Query: 414 VPVIEQYSRSCFLVWXSTFF 355
+P++E+Y+++C +W S+ F
Sbjct: 37 LPIVEKYAKNCNTIWDSSKF 56
>SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 520
Score = 25.8 bits (54), Expect = 4.7
Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Frame = +1
Query: 247 YPTRANKQTLQDLYRIPDFSTPQEFFAQLAQRMGRFKKGGVPDQE--AASRILLN 405
YP + K L+ ++PD +E+ A + G V D + A++R+L+N
Sbjct: 351 YPEKVTKALLELYPKVPDMEDEKEYLAAIKALFGSI----VSDMQVYASTRVLIN 401
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 25.8 bits (54), Expect = 4.7
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -3
Query: 311 GVLKSGILYKSCKVCLLALV 252
G+L ILYK CK LL+L+
Sbjct: 521 GILTYEILYKKCKCSLLSLL 540
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,284,181
Number of Sequences: 5004
Number of extensions: 44746
Number of successful extensions: 130
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 254167452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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