Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0541
(589 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g07050.1 68416.m00837 GTP-binding family protein contains Pfa... 94 5e-20
At1g52980.1 68414.m05995 GTP-binding family protein contains Pfa... 69 2e-12
At4g02790.1 68417.m00379 GTP-binding family protein contains Pfa... 56 2e-08
At1g08410.1 68414.m00930 GTP-binding family protein contains Pfa... 55 4e-08
At2g27200.1 68415.m03269 GTP-binding family protein contains Pfa... 54 7e-08
At2g41670.1 68415.m05148 GTP-binding family protein contains Pfa... 45 4e-05
At5g11480.1 68418.m01340 expressed protein 38 0.004
At5g58370.2 68418.m07309 expressed protein 38 0.007
At5g58370.1 68418.m07308 expressed protein 38 0.007
At3g12080.1 68416.m01504 GTP-binding family protein contains Pfa... 34 0.081
At1g78010.1 68414.m09091 tRNA modification GTPase, putative simi... 34 0.081
At2g22870.1 68415.m02715 expressed protein 33 0.19
At5g39960.1 68418.m04846 GTP-binding family protein contains Pfa... 32 0.33
At1g50920.1 68414.m05725 GTP-binding protein-related similar to ... 29 1.7
At3g57180.1 68416.m06366 expressed protein 29 2.3
At1g80770.1 68414.m09476 expressed protein 29 2.3
At4g38790.1 68417.m05492 ER lumen protein retaining receptor fam... 29 3.0
At3g05630.1 68416.m00626 phospholipase D, putative (PLDP2) ident... 29 3.0
At1g56710.1 68414.m06522 glycoside hydrolase family 28 protein /... 28 4.0
At1g10300.1 68414.m01160 GTP-binding protein-related contains si... 28 5.3
At3g60020.1 68416.m06702 E3 ubiquitin ligase SCF complex subunit... 27 7.0
At2g36760.1 68415.m04509 UDP-glucoronosyl/UDP-glucosyl transfera... 27 7.0
At1g76580.1 68414.m08911 SPL1-Related3 protein (SPL1R3) identica... 27 9.3
>At3g07050.1 68416.m00837 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 582
Score = 94.3 bits (224), Expect = 5e-20
Identities = 41/82 (50%), Positives = 58/82 (70%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDSTVA 190
PNVGKSS+INSL R+ NVG+TPG+T+ +Q V LD +K+LD PG+V +D+++A
Sbjct: 262 PNVGKSSLINSLKRAHVVNVGATPGLTRSLQEVHLDKNVKLLDCPGVVMLKSSGNDASIA 321
Query: 191 LKNAIRVGSLKDPVTPATAILR 256
L+N R+ L DPV+P IL+
Sbjct: 322 LRNCKRIEKLDDPVSPVKEILK 343
Score = 62.1 bits (144), Expect = 3e-10
Identities = 27/60 (45%), Positives = 39/60 (65%)
Frame = +1
Query: 265 KQTLQDLYRIPDFSTPQEFFAQLAQRMGRFKKGGVPDQEAASRILLNDWNIGXVRYFTEP 444
K L LY+IP F +F ++A G+ KKGG+ D +AA+RI+L+DWN G + Y+T P
Sbjct: 347 KDMLVTLYKIPSFEAVDDFLYKVATVRGKLKKGGLVDIDAAARIVLHDWNEGKIPYYTMP 406
>At1g52980.1 68414.m05995 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 576
Score = 69.3 bits (162), Expect = 2e-12
Identities = 33/82 (40%), Positives = 50/82 (60%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDSTVA 190
PNVGKSS+IN+L C V PG TK Q + L +I ++D PG+V+QS ++++ +
Sbjct: 318 PNVGKSSVINTLRTKNVCKVAPIPGETKVWQYITLTKRIFLIDCPGVVYQS-RDTETDIV 376
Query: 191 LKNAIRVGSLKDPVTPATAILR 256
LK +RV +L+D +LR
Sbjct: 377 LKGVVRVTNLEDASEHIGEVLR 398
Score = 54.0 bits (124), Expect = 7e-08
Identities = 22/63 (34%), Positives = 35/63 (55%)
Frame = +1
Query: 256 RANKQTLQDLYRIPDFSTPQEFFAQLAQRMGRFKKGGVPDQEAASRILLNDWNIGXVRYF 435
R K+ LQ Y+I D+ +F QL + G+ KGG PD ++++L+DW G + +F
Sbjct: 399 RVKKEHLQRAYKIKDWEDDHDFLLQLCKSSGKLLKGGEPDLMTGAKMILHDWQRGRIPFF 458
Query: 436 TEP 444
P
Sbjct: 459 VPP 461
>At4g02790.1 68417.m00379 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 372
Score = 55.6 bits (128), Expect = 2e-08
Identities = 29/65 (44%), Positives = 40/65 (61%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDSTVA 190
PNVGKSS+IN L + K C PGVT++M+ V+L + +LDSPG++ D A
Sbjct: 222 PNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLDSPGMLPMR--IDDQAAA 279
Query: 191 LKNAI 205
+K AI
Sbjct: 280 IKLAI 284
>At1g08410.1 68414.m00930 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 589
Score = 54.8 bits (126), Expect = 4e-08
Identities = 26/51 (50%), Positives = 33/51 (64%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQS 163
PNVGKSS IN+L K V STPG TK QT+ + ++ + D PG+VF S
Sbjct: 317 PNVGKSSTINALVGQKRTGVTSTPGKTKHFQTLIISDELMLCDCPGLVFPS 367
>At2g27200.1 68415.m03269 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 537
Score = 54.0 bits (124), Expect = 7e-08
Identities = 26/51 (50%), Positives = 32/51 (62%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQS 163
PNVGKSS IN+L K V STPG TK QT+ + + + D PG+VF S
Sbjct: 313 PNVGKSSTINALVGQKRTGVTSTPGKTKHFQTLIISEDLMLCDCPGLVFPS 363
>At2g41670.1 68415.m05148 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 386
Score = 44.8 bits (101), Expect = 4e-05
Identities = 34/87 (39%), Positives = 48/87 (55%), Gaps = 13/87 (14%)
Frame = +2
Query: 11 PNVGKSSIINSLN-----------RSKACNVGSTPGVTKQMQTVQLDSK--IKILDSPGI 151
PNVGKS++INS++ R K VG PGVT+ + ++ + I +LDSPG+
Sbjct: 145 PNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGFKIAHRPSIYVLDSPGV 204
Query: 152 VFQSGPESDSTVALKNAIRVGSLKDPV 232
+ S P D LK A+ GS+KD V
Sbjct: 205 LVPSIP--DIETGLKLALS-GSVKDSV 228
>At5g11480.1 68418.m01340 expressed protein
Length = 318
Score = 38.3 bits (85), Expect = 0.004
Identities = 21/53 (39%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = +2
Query: 14 NVGKSSIINSLNRSKACNVGS-TPGVTKQMQTVQLDSKIKILDSPGIVFQSGP 169
NVGKSS++NSL R K + S PG T+ + +++ K ++D PG + S P
Sbjct: 145 NVGKSSLLNSLVRRKRLALTSKKPGKTQCINHFRINDKWYLVDLPGYGYASAP 197
>At5g58370.2 68418.m07309 expressed protein
Length = 465
Score = 37.5 bits (83), Expect = 0.007
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = +2
Query: 14 NVGKSSIINSLNRS-KACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVF 157
NVGKSS++N+L R PG+T+ + L K++++D PG F
Sbjct: 298 NVGKSSLLNALTRQWGVVRTSDKPGLTQTINFFGLGPKVRLVDLPGYGF 346
>At5g58370.1 68418.m07308 expressed protein
Length = 446
Score = 37.5 bits (83), Expect = 0.007
Identities = 18/49 (36%), Positives = 28/49 (57%), Gaps = 1/49 (2%)
Frame = +2
Query: 14 NVGKSSIINSLNRS-KACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVF 157
NVGKSS++N+L R PG+T+ + L K++++D PG F
Sbjct: 298 NVGKSSLLNALTRQWGVVRTSDKPGLTQTINFFGLGPKVRLVDLPGYGF 346
>At3g12080.1 68416.m01504 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 663
Score = 33.9 bits (74), Expect = 0.081
Identities = 28/100 (28%), Positives = 45/100 (45%), Gaps = 9/100 (9%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQL----DSKIKILDSPGI-----VFQS 163
PNVGKSSI+N+L R V G T+ + K +++D+ GI V S
Sbjct: 377 PNVGKSSILNALVREDRTIVSPVSGTTRDAIDAEFTGPDGEKFRLIDTAGIRKKSSVASS 436
Query: 164 GPESDSTVALKNAIRVGSLKDPVTPATAILRGLTSKPYKI 283
G +++ +++ A R D V + +T + KI
Sbjct: 437 GSTTEA-MSVNRAFRAIRRSDVVALVIEAMACITEQDLKI 475
Score = 31.5 bits (68), Expect = 0.43
Identities = 23/89 (25%), Positives = 40/89 (44%), Gaps = 3/89 (3%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQM---QTVQLDSKIKILDSPGIVFQSGPESDS 181
PNVGKS++ N L V PGVT+ ++ D + ++D+ G++ S S
Sbjct: 167 PNVGKSALFNRLVGENRAIVVDEPGVTRDRLYGRSYWGDQEFVVVDTGGVMTVSKSPSGV 226
Query: 182 TVALKNAIRVGSLKDPVTPATAILRGLTS 268
L + +G P++ A + + S
Sbjct: 227 MEELNVSTTIGMEGIPLSSREAAIARMPS 255
>At1g78010.1 68414.m09091 tRNA modification GTPase, putative similar
to tRNA modification GTPase trmE [strain PCC 7120,
Anabaena sp.] SWISS-PROT:Q8YN91
Length = 560
Score = 33.9 bits (74), Expect = 0.081
Identities = 22/100 (22%), Positives = 49/100 (49%), Gaps = 3/100 (3%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSK---IKILDSPGIVFQSGPESDS 181
PNVGKSS++N+ ++S+ V G T+ + + + I +LD+ GI + +
Sbjct: 326 PNVGKSSLLNAWSKSERAIVTEVAGTTRDVVEANVTVRGVPITLLDTAGI--RETNDIVE 383
Query: 182 TVALKNAIRVGSLKDPVTPATAILRGLTSKPYKICIEYQT 301
+ ++ + + D + A + + G T + ++ + Q+
Sbjct: 384 KIGVERSETAAKVADVIIMAVSAVEGWTEEDTELLRKIQS 423
>At2g22870.1 68415.m02715 expressed protein
Length = 300
Score = 32.7 bits (71), Expect = 0.19
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = +2
Query: 14 NVGKSSIINSLNRSKACNVGS-TPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDST 184
NVGKSS+IN L R K + S PG T+ + ++ I+D PG F ++ T
Sbjct: 130 NVGKSSLINCLVRKKEVALTSKKPGKTQLINHFLVNKSWYIVDLPGYGFAKVSDAAKT 187
>At5g39960.1 68418.m04846 GTP-binding family protein contains Pfam
domain, PF01926: GTPase of unknown function
Length = 616
Score = 31.9 bits (69), Expect = 0.33
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLD 118
PNVGKS+++N+L + VG G+T+ VQ +
Sbjct: 321 PNVGKSTLLNALLEEERVLVGPEAGLTRDAVRVQFE 356
>At1g50920.1 68414.m05725 GTP-binding protein-related similar to
GTP-binding protein SP:Q99ME9 from [Mus musculus]
Length = 671
Score = 29.5 bits (63), Expect = 1.7
Identities = 18/51 (35%), Positives = 28/51 (54%), Gaps = 3/51 (5%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSK---IKILDSPGIV 154
PNVGKSS +N + R+ +V TK + D K +++D+PGI+
Sbjct: 177 PNVGKSSFMNKVTRADV-DVQPYAFTTKSLFVGHTDYKYLRYQVIDTPGIL 226
>At3g57180.1 68416.m06366 expressed protein
Length = 644
Score = 29.1 bits (62), Expect = 2.3
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 7/54 (12%)
Frame = +2
Query: 14 NVGKSSIINSLNRSKACNV-----GSTPGVTKQMQTVQ--LDSKIKILDSPGIV 154
N GKS++IN+L++ V PG T + + L +K K+ D+PG++
Sbjct: 386 NAGKSTLINALSKKDGAKVTRLTEAPVPGTTLGILKIGGILSAKAKMYDTPGLL 439
>At1g80770.1 68414.m09476 expressed protein
Length = 451
Score = 29.1 bits (62), Expect = 2.3
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = +2
Query: 8 SPNVGKSSIINSLNRSK--ACNVGSTPGVTKQMQTVQLDSKIKILDSPGIVFQSGPESDS 181
+PNVGKSS++ L+ K CN T V + ++ D+PG++ + + ++
Sbjct: 261 APNVGKSSLVRILSTGKPEICNYPFTTRGILMGHIVLNYQRFQVTDTPGLLRRCDEDRNN 320
Query: 182 TVALKNAI 205
L A+
Sbjct: 321 LEKLTLAV 328
>At4g38790.1 68417.m05492 ER lumen protein retaining receptor family
protein similar to SP|P35402 ER lumen protein retaining
receptor (HDEL receptor) {Arabidopsis thaliana};
contains Pfam profile PF00810: ER lumen protein
retaining receptor
Length = 273
Score = 28.7 bits (61), Expect = 3.0
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = -2
Query: 147 PGLSRILIFESSWTVCICLVTPGVLPTLQALDRFKLL 37
P S +I SW +C+ L VLP L+ + K++
Sbjct: 155 PSTSHNIINRISWALCVYLEAVSVLPQLRVMQNTKIV 191
>At3g05630.1 68416.m00626 phospholipase D, putative (PLDP2)
identical to SP|Q9M9W8 Phospholipase D p2 (EC 3.1.4.4)
(AtPLDp2) (Phospholipase D2 PHOX and PX containing
domain) (Phospholipase D zeta 2) (PLDzeta2) {Arabidopsis
thaliana}; similar to phospholipase D GB:BAA24577 from
[Rattus norvegicus]; contains Pfam profile: PF00614
phospholipase D, PF00169 PH domain, PF00787 PX domain
Length = 1046
Score = 28.7 bits (61), Expect = 3.0
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = -2
Query: 123 FESSWTVCICLVTPGVLPTLQALDRFKLLIIELFPTFGL 7
F +SWT ++ PG L L+ KLL I +F T GL
Sbjct: 248 FGTSWTKVWAVLKPGFLALLEDPFSGKLLDIMVFDTLGL 286
>At1g56710.1 68414.m06522 glycoside hydrolase family 28 protein /
polygalacturonase (pectinase) family protein similar to
polygalacturonase PG1 GI:5669846, PG2 GI:5669848 from
(Glycine max); contains PF00295: Glycosyl hydrolases
family 28 (polygalacturonases)
Length = 434
Score = 28.3 bits (60), Expect = 4.0
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = +1
Query: 136 GQSWYSIPIRPGE*FNGSTQ 195
GQ W+++P +P + NG+TQ
Sbjct: 154 GQKWWNLPCKPHKGLNGTTQ 173
>At1g10300.1 68414.m01160 GTP-binding protein-related contains
similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4
from [Homo sapiens];
Length = 687
Score = 27.9 bits (59), Expect = 5.3
Identities = 17/51 (33%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = +2
Query: 11 PNVGKSSIINSLNRSKACNVGSTPGVTKQMQTVQLDSK---IKILDSPGIV 154
PNVGKSS +N + R+ V TK + D K +++D+PG++
Sbjct: 206 PNVGKSSFMNKVTRADVA-VQPYAFTTKSLFLGHTDYKCLRYQVIDTPGLL 255
>At3g60020.1 68416.m06702 E3 ubiquitin ligase SCF complex subunit
SKP1/ASK1 (At5), putative E3 ubiquitin ligase; similar
to Skp1 GI:4959710 from [Medicago sativa]
Length = 153
Score = 27.5 bits (58), Expect = 7.0
Identities = 23/79 (29%), Positives = 35/79 (44%)
Frame = +2
Query: 113 LDSKIKILDSPGIVFQSGPESDSTVALKNAIRVGSLKDPVTPATAILRGLTSKPYKICIE 292
+ +KI + S G F E D VA K+ ++D LR +TSK KI I+
Sbjct: 1 MSTKIMLKSSDGKSF----EIDEDVARKSIAINHMVEDGCATDVIPLRNVTSKILKIVID 56
Query: 293 YQTSVHLRNSLRN*HNEWE 349
Y H+++ EW+
Sbjct: 57 Y-CEKHVKSKEEEDLKEWD 74
>At2g36760.1 68415.m04509 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 496
Score = 27.5 bits (58), Expect = 7.0
Identities = 14/48 (29%), Positives = 23/48 (47%)
Frame = -2
Query: 480 RPTLXISVXCLGRLREIAYXTNVPVIEQYSRSCFLVWXSTFFEPSHSL 337
+P+ IS CL +IA N+P I + SCF + +H++
Sbjct: 123 KPSCLISDFCLPYTSKIAKRFNIPKIVFHGVSCFCLLSMHILHRNHNI 170
>At1g76580.1 68414.m08911 SPL1-Related3 protein (SPL1R3) identical
to cDNA partial mRNA for SPL1-Related3 protein (SPL1R3
gene) GI:6006428
Length = 488
Score = 27.1 bits (57), Expect = 9.3
Identities = 24/91 (26%), Positives = 44/91 (48%), Gaps = 10/91 (10%)
Frame = +2
Query: 26 SSIINSLNRSKACNVGSTPGVTKQMQTVQLDSKIKILDSP----------GIVFQSGPES 175
++++ + R++A GS PGV ++ Q +Q+ +KIK L P GI+ + PE
Sbjct: 8 TALVCAQGRNEATTNGS-PGVPQREQLLQILNKIKALPLPMNLTSKLNNIGILARKNPEQ 66
Query: 176 DSTVALKNAIRVGSLKDPVTPATAILRGLTS 268
S + +N++ S + A+ L S
Sbjct: 67 PSPMNPQNSMNGASSPSTMDLLAALSASLGS 97
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,831,649
Number of Sequences: 28952
Number of extensions: 231693
Number of successful extensions: 601
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 580
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 595
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1161268208
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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