Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0466
         (418 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces ...    64   1e-11
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos...    29   0.38 
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ...    27   1.2  
SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces po...    26   2.0  
SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex ...    25   4.7  
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ...    25   6.2  
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos...    25   6.2  
SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6 |Schi...    25   6.2  

>SPCC576.08c |rps2||40S ribosomal protein S2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 253

 Score = 63.7 bits (148), Expect = 1e-11
 Identities = 34/75 (45%), Positives = 47/75 (62%)
 Frame = +3

Query: 150 KEDQKEWVPVTKLGRLVREGKIDKLESIYLFSLPIKNSRSLIXFSARP*MMRFLRSCLYR 329
           ++++KEWVPVTKLGRLV+ GKI  +E IYL+SLPIK  + +  F  R      ++    +
Sbjct: 28  RDEEKEWVPVTKLGRLVKAGKIKSIEEIYLYSLPIKEYQIVDYFLPRL-NDEVMKVVPVQ 86

Query: 330 NKHVPDXXTRFKAFV 374
            +      TRFKAFV
Sbjct: 87  KQTRAGQRTRFKAFV 101


>SPBC17D11.05 |tif32||translation initiation factor
           eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 932

 Score = 28.7 bits (61), Expect = 0.38
 Identities = 12/31 (38%), Positives = 19/31 (61%)
 Frame = +3

Query: 243 SLPIKNSRSLIXFSARP*MMRFLRSCLYRNK 335
           ++ +K SR+   FS  P MMRF+  C++  K
Sbjct: 34  TIVLKRSRNAQGFSLEPIMMRFIELCVHLRK 64


>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 500

 Score = 27.1 bits (57), Expect = 1.2
 Identities = 17/43 (39%), Positives = 21/43 (48%), Gaps = 5/43 (11%)
 Frame = -1

Query: 403 KYDRCCRQWQTNALKRVXXSGTCLFLYRHD-----LKNLIIQG 290
           KYDR  R W+      +  S  CL LY +      LKNLI+ G
Sbjct: 9   KYDRQVRLWKAEGQNAIEKSHVCL-LYANTVGCEALKNLILPG 50


>SPBC18H10.11c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 432

 Score = 26.2 bits (55), Expect = 2.0
 Identities = 16/58 (27%), Positives = 28/58 (48%)
 Frame = -1

Query: 334 LFLYRHDLKNLIIQGRAEKXINDLEFLIGKENK*MLSSLSIFPSRTRRPSLVTGTHSF 161
           L +Y   L+NL+ QG+A + +N ++ +          +  I  S  +RP   + T SF
Sbjct: 361 LQVYHEKLRNLVQQGQAAECLNTIKRMSHNGPFPTQQTFLIVLSLCKRPKFYSYTKSF 418


>SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex
           subunit Apc3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 665

 Score = 25.0 bits (52), Expect = 4.7
 Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
 Frame = -1

Query: 400 YDRCCRQWQTNALKRVXXSGTCLFLYRHDLKNLIIQGRAEKXIND---LEFLIGK 245
           YDR C+  + ++L R   +   + L+ HD K L+   + +    D   + FL+GK
Sbjct: 556 YDRACKLDEKSSLARFKKAKVLILLHDHD-KALVELEQLKAIAPDEANVHFLLGK 609


>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1420

 Score = 24.6 bits (51), Expect = 6.2
 Identities = 12/42 (28%), Positives = 20/42 (47%)
 Frame = -2

Query: 147 RAHDHGRDHDRVHEDRHGLYLHRVIRIRRENRHVHRLEQRXP 22
           ++HDHG  H + H DR      +  R  R++R     ++  P
Sbjct: 720 QSHDHGHSHSKSH-DREKEKEKKKDREHRKHRETEEEDEGPP 760


>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 467

 Score = 24.6 bits (51), Expect = 6.2
 Identities = 17/68 (25%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
 Frame = +2

Query: 200 SRRKNRQTREHLLVFFTNQEF--EIIDXFLGPSLNDEVLKIMPVQKQTRAXXXHTFQGIC 373
           + R N+ +R+HL    T++EF    ID F    + D +  I+    QT+       QG  
Sbjct: 167 NNRGNKYSRQHLRFDSTDKEFWDFSIDDFAQYDIPDTIDYILKTSGQTKLTYIGFSQGTA 226

Query: 374 LPLSTTTV 397
              ++ ++
Sbjct: 227 QAFASLSI 234


>SPAC343.19 ||SPAC824.01|phosphatidylinositol 4-kinase Lsb6
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 624

 Score = 24.6 bits (51), Expect = 6.2
 Identities = 9/25 (36%), Positives = 18/25 (72%)
 Frame = +2

Query: 209 KNRQTREHLLVFFTNQEFEIIDXFL 283
           ++R+TRE+     ++++F+I D FL
Sbjct: 289 RHRETREYTESVASSEDFDIFDPFL 313


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,383,484
Number of Sequences: 5004
Number of extensions: 23221
Number of successful extensions: 66
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 64
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 65
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 146319408
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)