Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0436
         (487 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g39630.1 68418.m04799 vesicle transport v-SNARE family protei...    37   0.006
At1g26670.1 68414.m03249 vesical transport v-SNARE 12 (VTI12) / ...    33   0.13 
At5g39510.1 68418.m04784 vesicle transport v-SNARE 11 (VTI11) / ...    31   0.31 
At2g28520.1 68415.m03465 vacuolar proton ATPase, putative simila...    29   1.7  
At1g21650.1 68414.m02710 preprotein translocase secA family prot...    29   1.7  
At5g43110.1 68418.m05263 pumilio/Puf RNA-binding domain-containi...    28   3.8  
At4g12750.1 68417.m02002 expressed protein                             28   3.8  
At5g03180.1 68418.m00266 zinc finger (C3HC4-type RING finger) fa...    27   8.9  
At4g24680.1 68417.m03533 expressed protein                             27   8.9  
At3g58010.1 68416.m06465 expressed protein                             27   8.9  
At3g09980.1 68416.m01198 expressed protein contains Pfam profile...    27   8.9  
At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase fa...    27   8.9  
At1g13120.1 68414.m01521 expressed protein contains Prosite PS00...    27   8.9  

>At5g39630.1 68418.m04799 vesicle transport v-SNARE family protein
           similar to v-SNARE AtVTI1a (GI:10177700) Arabidopsis
           thaliana; contains Pfam profile PF05008: Vesicle
           transport v-SNARE protein
          Length = 207

 Score = 37.1 bits (82), Expect = 0.006
 Identities = 18/44 (40%), Positives = 26/44 (59%)
 Frame = +2

Query: 254 SEQIGIATAEELSRQREQLQNTDRRLDEINTNLNYSQKHLNGIK 385
           +E IGI+  E L RQ+E LQN+   L EI+  +  S+  +  IK
Sbjct: 143 TENIGISILENLQRQKESLQNSQAMLHEIDDTVKESRSIVRSIK 186


>At1g26670.1 68414.m03249 vesical transport v-SNARE 12 (VTI12) /
           vesicle soluble NSF attachment protein receptor VTI1b
           (VTI1B) receptor VTI1b identical to SP|Q9SEL5 Vesicle
           transport v-SNARE 12 (AtVTI12) (Vesicle transport
           v-SNARE protein VTI1b) (Vesicle soluble NSF attachment
           protein receptor VTI1b) (AtVTI1b) {Arabidopsis thaliana}
          Length = 222

 Score = 32.7 bits (71), Expect = 0.13
 Identities = 15/53 (28%), Positives = 32/53 (60%)
 Frame = +2

Query: 224 INEKYRIAS*SEQIGIATAEELSRQREQLQNTDRRLDEINTNLNYSQKHLNGI 382
           I E  R+   +E++GI+  ++LS+QR+ L +   +L  ++  ++ S+K L  +
Sbjct: 138 IRESRRLMLETEEVGISIVQDLSQQRQTLLHAHNKLHGVDDAIDKSKKVLTAM 190


>At5g39510.1 68418.m04784 vesicle transport v-SNARE 11 (VTI11) /
           vesicle soluble NSF attachment protein receptor VTI1a
           (VTI1A) identical to SP|Q9SEL6 Vesicle transport v-SNARE
           11 (AtVTI11) (Vesicle transport v-SNARE protein VTI1a)
           (Vesicle soluble NSF attachment protein receptor VTI1a)
           (AtVTI1a) {Arabidopsis thaliana}
          Length = 221

 Score = 31.5 bits (68), Expect = 0.31
 Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 1/63 (1%)
 Frame = +2

Query: 188 NTKR-NRTTDNRFINEKYRIAS*SEQIGIATAEELSRQREQLQNTDRRLDEINTNLNYSQ 364
           +T+R  RTTD   + +  R    +E+IG++  ++L  QR+ L      L  ++ N+  S+
Sbjct: 126 STERLGRTTDR--VKDSRRTMMETEEIGVSILQDLHGQRQSLLRAHETLHGVDDNIGKSK 183

Query: 365 KHL 373
           K L
Sbjct: 184 KIL 186


>At2g28520.1 68415.m03465 vacuolar proton ATPase, putative similar
           to Swiss-Prot:Q93050 vacuolar proton translocating
           ATPase 116 kDa subunit A isoform 1 (Clathrin-coated
           vesicle/synaptic vesicle proton pump 116 kDa subunit,
           Vacuolar proton pump subunit 1, Vacuolar adenosine
           triphosphatase subunit Ac116) [Homo sapiens]
          Length = 817

 Score = 29.1 bits (62), Expect = 1.7
 Identities = 14/47 (29%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
 Frame = +2

Query: 281 EELSRQREQLQNTDRRLDEINTNLNYSQKHL-NGIKSVFYGLKNYIS 418
           E+ ++QR+  +    RL ++   L+   +H  N + SV Y L N+I+
Sbjct: 271 EDTTKQRQLTREVLSRLSDLEATLDAGTRHRNNALNSVGYSLTNWIT 317


>At1g21650.1 68414.m02710 preprotein translocase secA family protein
           contains Pfam profiles: PF01043 SecA protein, amino
           terminal region, PF00400 WD domain, G-beta repeat,
           PF00097 zinc finger, C3HC4 type (RING finger)
          Length = 1579

 Score = 29.1 bits (62), Expect = 1.7
 Identities = 16/32 (50%), Positives = 19/32 (59%)
 Frame = +3

Query: 195 REIEQRTIDSSTRSIGLLRDLNKLVLQRPKSF 290
           RE E    DSS+R  G LRD +K+ L R  SF
Sbjct: 122 RESESSDFDSSSRLCGSLRDDSKVSLLRVASF 153


>At5g43110.1 68418.m05263 pumilio/Puf RNA-binding domain-containing
           protein contains similarity to RNA-binding protein
          Length = 518

 Score = 27.9 bits (59), Expect = 3.8
 Identities = 15/43 (34%), Positives = 24/43 (55%)
 Frame = +3

Query: 144 GHIAELERQRQAMIQTQREIEQRTIDSSTRSIGLLRDLNKLVL 272
           G+ A L R +      Q+ ++ R IDS    I LLR+++ L+L
Sbjct: 427 GNYAHLARNKYGSHAVQKLLKLRWIDSRVIVIDLLREIDTLLL 469


>At4g12750.1 68417.m02002 expressed protein 
          Length = 1108

 Score = 27.9 bits (59), Expect = 3.8
 Identities = 19/58 (32%), Positives = 32/58 (55%), Gaps = 1/58 (1%)
 Frame = +3

Query: 108 IPQTQQANKPYGGHIAELERQRQ-AMIQTQREIEQRTIDSSTRSIGLLRDLNKLVLQR 278
           +PQ+   N P+     + ++QRQ A I  Q+ +++R+ +   RSI    +LNK   QR
Sbjct: 186 LPQSSARNPPH-----QKKKQRQLASILKQKLLQKRSTEKKRRSIHREAELNKDETQR 238


>At5g03180.1 68418.m00266 zinc finger (C3HC4-type RING finger)
           family protein various predicted proteins, Arabidopsis
           thaliana ; contains Pfam profile PF00097: Zinc finger,
           C3HC4 type (RING finger)
          Length = 462

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 11/28 (39%), Positives = 14/28 (50%)
 Frame = +2

Query: 155 RTGTSTPSYDPNTKRNRTTDNRFINEKY 238
           RTG    S  P++ RNRT    F N  +
Sbjct: 86  RTGRRVGSMSPSSSRNRTNQKNFKNRNH 113


>At4g24680.1 68417.m03533 expressed protein
          Length = 1480

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 14/36 (38%), Positives = 21/36 (58%)
 Frame = +3

Query: 156 ELERQRQAMIQTQREIEQRTIDSSTRSIGLLRDLNK 263
           EL RQR    Q Q+E E+R  D   +++  L +LN+
Sbjct: 744 ELARQRAQ--QRQKEEEERARDQRAKALAKLEELNR 777


>At3g58010.1 68416.m06465 expressed protein
          Length = 308

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 8/28 (28%), Positives = 17/28 (60%)
 Frame = -1

Query: 478 TSITYIXGRWTFRWCLITFSGNIILQTI 395
           T+  Y+ GRW+F W  +   G++ ++ +
Sbjct: 123 TTSPYLEGRWSFEWFGVNTPGSLAVRVM 150


>At3g09980.1 68416.m01198 expressed protein contains Pfam profile
           PF04949: Family of unknown function (DUF662)
          Length = 178

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 13/68 (19%), Positives = 33/68 (48%)
 Frame = +3

Query: 105 RIPQTQQANKPYGGHIAELERQRQAMIQTQREIEQRTIDSSTRSIGLLRDLNKLVLQRPK 284
           +I    +  KP G  + + ER+ +  ++   E  +  +   TR + L+ +  K+ +++ +
Sbjct: 109 KIDSVNKELKPLGHTVQKKEREYKEALEAFNEKNREKVQLITRLMELVGESEKMRMKKLE 168

Query: 285 SFPDNVNS 308
               N++S
Sbjct: 169 ELSKNIDS 176


>At1g13640.1 68414.m01603 phosphatidylinositol 3- and 4-kinase
           family protein low similarity to phosphatidylinositol
           4-kinase type-II beta [Homo sapiens] GI:20159767;
           contains Pfam profile PF00454: Phosphatidylinositol 3-
           and 4-kinase
          Length = 622

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 14/49 (28%), Positives = 26/49 (53%)
 Frame = +1

Query: 34  SQIRTTYSKMTTSMITNL*IASGQEYLKHSKQISLMEVILQNWNVNAKL 180
           +++  + SK++TSM  NL    G  YLK  K     + ++ + + N +L
Sbjct: 522 AKLTLSLSKLSTSMKNNLSNTMGSGYLKPPKDNQTDKALVSHKSANVQL 570


>At1g13120.1 68414.m01521 expressed protein contains Prosite
           PS00012: Phosphopantetheine attachment site; similar to
           GLE1 (GI:3288817) {Homo sapiens}; EST gb|N37870 comes
           from this gene
          Length = 611

 Score = 26.6 bits (56), Expect = 8.9
 Identities = 13/45 (28%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
 Frame = +2

Query: 293 RQREQLQNTDRRL-DEINTNLNYSQKHLNGIKSVFYGLKNYISGK 424
           ++ E+L+ T++ L    N N +  +KH+  +     G K+ +SGK
Sbjct: 330 KKLEELETTNQSLKSRSNENFSSFEKHIGRVIRQISGTKDSVSGK 374


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,802,593
Number of Sequences: 28952
Number of extensions: 181901
Number of successful extensions: 558
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 542
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 558
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 838967680
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)