BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0435 (488 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces... 48 6e-07 SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIII... 48 8e-07 SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pom... 48 1e-06 SPAC4G8.13c |prz1||transcription factor Prz1 |Schizosaccharomyce... 47 2e-06 SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyce... 45 5e-06 SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces... 42 4e-05 SPAC25B8.19c ||SPAC683.01c|transcription factor, zf-fungal binuc... 41 1e-04 SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces... 40 2e-04 SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyce... 40 2e-04 SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 39 5e-04 SPAC144.02 |||transcription factor |Schizosaccharomyces pombe|ch... 38 8e-04 SPAC11D3.17 |||zinc finger protein|Schizosaccharomyces pombe|chr... 34 0.010 SPAC6B12.08 |mug185||DNAJ domain protein Jjj family|Schizosaccha... 31 0.071 SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces ... 29 0.28 SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su... 29 0.50 SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB... 28 0.66 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 27 1.1 SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 27 1.5 SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 27 2.0 SPBC19G7.04 |||HMG box protein |Schizosaccharomyces pombe|chr 2|... 26 3.5 SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc... 25 4.6 SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase ... 25 4.6 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 25 4.6 SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 25 6.1 SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pomb... 25 6.1 SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 25 8.1 SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces ... 25 8.1 >SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces pombe|chr 2|||Manual Length = 897 Score = 48.4 bits (110), Expect = 6e-07 Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 2/54 (3%) Frame = +1 Query: 28 YICQY--CNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKRRH 183 + C Y C K+F K+ L+ HER H K +SC FC+ F N+H+++ H Sbjct: 26 FYCTYPDCPKSFTRKEHLRRHERTHENVKAFSCSFCNRAFARSDVLNRHVQQMH 79 >SPAC144.09c |sfc2||RNA polymerase III transcription factor TFIIIA|Schizosaccharomyces pombe|chr 1|||Manual Length = 374 Score = 48.0 bits (109), Expect = 8e-07 Identities = 21/58 (36%), Positives = 34/58 (58%), Gaps = 4/58 (6%) Frame = +1 Query: 13 ADSMPYICQY--CNKAFRFKQILKNHERQHTGSKPYSCQF--CSMEFTNWSNYNKHMK 174 ++ P++C Y C+KAF K LK H+R HT KP+SC + C +F + +H++ Sbjct: 48 SNERPFVCDYTGCSKAFYRKSHLKIHKRCHTNVKPFSCHYDGCDAQFYTQQHLERHIE 105 Score = 35.1 bits (77), Expect = 0.006 Identities = 22/61 (36%), Positives = 26/61 (42%), Gaps = 7/61 (11%) Frame = +1 Query: 22 MPYICQY--CNKAFRFKQILKNH-ERQHTGSKPYSCQFCSME----FTNWSNYNKHMKRR 180 +PY C Y C F KQ L+NH R H YSC S F WS H++ Sbjct: 142 LPYPCTYQDCELRFATKQKLQNHVNRAHEKIISYSCPHESCVGHEGFEKWSQLQNHIREA 201 Query: 181 H 183 H Sbjct: 202 H 202 Score = 33.1 bits (72), Expect = 0.023 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%) Frame = +1 Query: 34 CQY--CNKAFRFKQILKNHERQHTGSKPYSCQF--CSMEFTNWSNYNKHMKRRH 183 C Y C K + +L+ H R H+ +P+ C + CS F S+ H KR H Sbjct: 25 CPYEECGKKYSRPSLLEQHLRTHSNERPFVCDYTGCSKAFYRKSHLKIH-KRCH 77 Score = 29.5 bits (63), Expect = 0.28 Identities = 21/60 (35%), Positives = 26/60 (43%), Gaps = 2/60 (3%) Frame = +1 Query: 43 CNKAFRFKQILKNHERQ-HTGSKPYSCQFCSMEFTNWSNYNKHMKRRH-GTDTAKKKITP 216 C K+F LK H H G+ + C C +F Y KHM +RH T KK P Sbjct: 243 CKKSFTRSSALKKHISVIHEGNMAFHCDSCGTKF----GY-KHMLQRHLERGTCKKAHKP 297 >SPAC1039.05c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 781 Score = 47.6 bits (108), Expect = 1e-06 Identities = 20/54 (37%), Positives = 29/54 (53%), Gaps = 2/54 (3%) Frame = +1 Query: 28 YICQY--CNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKRRH 183 Y C + C K+F K+ + H R HT SK + C CS FT N+H+ ++H Sbjct: 17 YKCDFQGCTKSFTRKEHARRHFRSHTNSKAFICPHCSSSFTRSDVLNRHVNQKH 70 Score = 25.0 bits (52), Expect = 6.1 Identities = 8/29 (27%), Positives = 15/29 (51%) Frame = +1 Query: 7 ARADSMPYICQYCNKAFRFKQILKNHERQ 93 + +S +IC +C+ +F +L H Q Sbjct: 40 SHTNSKAFICPHCSSSFTRSDVLNRHVNQ 68 >SPAC4G8.13c |prz1||transcription factor Prz1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 681 Score = 46.8 bits (106), Expect = 2e-06 Identities = 18/54 (33%), Positives = 27/54 (50%) Frame = +1 Query: 25 PYICQYCNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKRRHG 186 P+ C C K+F + + HE+ HTG K ++C C+ F N+H K G Sbjct: 599 PFQCSICKKSFARQHDKRRHEQLHTGIKAFACVTCNQRFARMDALNRHYKSEVG 652 Score = 42.3 bits (95), Expect = 4e-05 Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 2/54 (3%) Frame = +1 Query: 28 YICQY--CNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKRRH 183 Y+C + CNK F LK+H HT +P+ C C F +H KRRH Sbjct: 570 YVCTFAGCNKRFTRAYNLKSHMNTHTNYRPFQCSICKKSFA-----RQHDKRRH 618 >SPBP4H10.09 |rsv1||transcription factor Rsv1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 45.2 bits (102), Expect = 5e-06 Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 2/72 (2%) Frame = +1 Query: 28 YICQYCNKAFRFKQILKNHERQHTGSKPYSCQF--CSMEFTNWSNYNKHMKRRHGTDTAK 201 Y C +C + F ++ H R HTG KP+ C + C FT +H++ T K Sbjct: 4 YECPFCKRVFHRQEHQVRHIRSHTGEKPFECSYPSCKKRFTRRDELIRHVR----THLRK 59 Query: 202 KKITPEGVFPVD 237 +TPE V+ Sbjct: 60 ALVTPEQTLDVN 71 >SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 565 Score = 42.3 bits (95), Expect = 4e-05 Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 2/52 (3%) Frame = +1 Query: 25 PYICQYCNKAFRFKQILKNHERQHTGSKPYSCQF--CSMEFTNWSNYNKHMK 174 PY C C KAF + H R HTG KP+ C F C+ F+ +H + Sbjct: 25 PYKCPLCTKAFYRLEHQTRHIRTHTGEKPHVCTFPGCAKRFSRSDELTRHAR 76 >SPAC25B8.19c ||SPAC683.01c|transcription factor, zf-fungal binuclear cluster type |Schizosaccharomyces pombe|chr 1|||Manual Length = 522 Score = 40.7 bits (91), Expect = 1e-04 Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 2/64 (3%) Frame = +1 Query: 1 NSARADSMPYICQYCNKAFRFKQILKNHERQHTGSKPYSCQF--CSMEFTNWSNYNKHMK 174 +++ A ++ Y C C + F LK H HTG +P+ C + C F SN +H + Sbjct: 459 STSSAANVRYRCTECLQGFSRPSSLKIHTYSHTGERPFVCDYAGCGKAFNVRSNMRRH-Q 517 Query: 175 RRHG 186 R HG Sbjct: 518 RIHG 521 >SPAC6F12.02 |rst2||transcription factor Rst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 567 Score = 40.3 bits (90), Expect = 2e-04 Identities = 14/31 (45%), Positives = 20/31 (64%) Frame = +1 Query: 28 YICQYCNKAFRFKQILKNHERQHTGSKPYSC 120 Y+C+ C +AF + LK H R HT KP++C Sbjct: 70 YVCETCTRAFARLEHLKRHIRSHTNEKPFTC 100 >SPAC6G10.12c |ace2||transcription factor Ace2|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 39.9 bits (89), Expect = 2e-04 Identities = 15/51 (29%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = +1 Query: 28 YICQY--CNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMK 174 Y+C Y CNK K +++H + H +PY C C F + +H++ Sbjct: 443 YVCLYNGCNKRIARKYNVESHIQTHLSDRPYRCDLCKAGFVRHHDLKRHLR 493 Score = 39.5 bits (88), Expect = 3e-04 Identities = 18/51 (35%), Positives = 22/51 (43%) Frame = +1 Query: 25 PYICQYCNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKR 177 PY C C F LK H R H +PY C+ C F N+H +R Sbjct: 472 PYRCDLCKAGFVRHHDLKRHLRIHENGRPYVCE-CLKRFNRLDALNRHKQR 521 >SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 582 Score = 38.7 bits (86), Expect = 5e-04 Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 1/53 (1%) Frame = +1 Query: 28 YICQYCNKAFRFKQILKNHERQ-HTGSKPYSCQFCSMEFTNWSNYNKHMKRRH 183 ++C C+K F+ + L+ H R HT KP+ C C F+ N +H +R H Sbjct: 423 FVCPECSKKFKRSEHLRRHIRSLHTSEKPFVC-ICGKRFSRRDNLRQH-ERLH 473 Score = 30.3 bits (65), Expect = 0.16 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 1/42 (2%) Frame = +1 Query: 4 SARADSMPYICQYCNKAFRFKQILKNHERQHTGSKP-YSCQF 126 S P++C C K F + L+ HER H + P +C F Sbjct: 444 SLHTSEKPFVC-ICGKRFSRRDNLRQHERLHVNASPRLACFF 484 >SPAC144.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 249 Score = 37.9 bits (84), Expect = 8e-04 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 2/55 (3%) Frame = +1 Query: 82 HERQHTGSKPYSCQF--CSMEFTNWSNYNKHMKRRHGTDTAKKKITPEGVFPVDP 240 H R HTG KP+ C C FT KHM+ H DT + P+ P Sbjct: 96 HLRSHTGEKPFICSVPECDRSFTRSDALAKHMRTVHEADTLRPSDPIPKAHPMHP 150 >SPAC11D3.17 |||zinc finger protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 585 Score = 34.3 bits (75), Expect = 0.010 Identities = 13/50 (26%), Positives = 24/50 (48%) Frame = +1 Query: 34 CQYCNKAFRFKQILKNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKRRH 183 C C K F + L H+ H+ ++P C +C ++ +H++R H Sbjct: 33 CDQCAKRFTRHENLTRHKACHSKAEPIPCPYCEIKCKRKDLLKRHIQRFH 82 >SPAC6B12.08 |mug185||DNAJ domain protein Jjj family|Schizosaccharomyces pombe|chr 1|||Manual Length = 380 Score = 31.5 bits (68), Expect = 0.071 Identities = 13/31 (41%), Positives = 17/31 (54%) Frame = +1 Query: 16 DSMPYICQYCNKAFRFKQILKNHERQHTGSK 108 +S P +C CNK FR + L+NHE K Sbjct: 266 NSNPIMCMVCNKNFRSQNQLENHENSKKHKK 296 >SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 463 Score = 29.5 bits (63), Expect = 0.28 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = +1 Query: 109 PYSCQFCSMEFTNWSNYNKHMKRRH 183 P C FC+ F+++ KHMK H Sbjct: 207 PRDCLFCAASFSSFDTCKKHMKASH 231 Score = 25.4 bits (53), Expect = 4.6 Identities = 11/43 (25%), Positives = 19/43 (44%) Frame = +1 Query: 76 KNHERQHTGSKPYSCQFCSMEFTNWSNYNKHMKRRHGTDTAKK 204 +N E Q +C+ C+ +F + Y+ HM + D K Sbjct: 58 QNEEVQKKAEFYQNCEVCNKKFYSEGAYSSHMASKKHRDNLSK 100 >SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 448 Score = 28.7 bits (61), Expect = 0.50 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +1 Query: 16 DSMPYICQYCNKAFRFKQILKNHERQHTGSKPYSCQFCSME 138 DS Y+C +CNK F +L + T ++C C E Sbjct: 132 DSKGYVCPFCNKKFSSLDVLSLVTNEGT----FACNVCGTE 168 >SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT |Schizosaccharomyces pombe|chr 1|||Manual Length = 588 Score = 28.3 bits (60), Expect = 0.66 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = -1 Query: 350 VNLFDSFLFPPFPGYHDLTLPLLSTGWVFYLYICP 246 +NLF F F +HD++ L + GW+ L+I P Sbjct: 455 LNLFIIFTFVAL--WHDISWELFAWGWLIVLFILP 487 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 27.5 bits (58), Expect = 1.1 Identities = 9/27 (33%), Positives = 13/27 (48%) Frame = +1 Query: 103 SKPYSCQFCSMEFTNWSNYNKHMKRRH 183 +KPY C+ CS + N + H H Sbjct: 396 NKPYRCEVCSKRYKNLNGLKYHRTHSH 422 >SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 697 Score = 27.1 bits (57), Expect = 1.5 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -1 Query: 314 PGYHDLTLPLLSTGWVFYLYICPVMGSTGKTPSGVI 207 P D + LL++ ++Y+ MGSTG + +GVI Sbjct: 157 PSTTDRRMELLASVADSFIYVVSRMGSTGSSATGVI 192 >SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 926 Score = 26.6 bits (56), Expect = 2.0 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +1 Query: 172 KRRHGTDTAKKKITPEGVFPVDPITGQM 255 K +G + I P+G PV P+ GQ+ Sbjct: 10 KGSYGASSGNSNIPPQGFQPVYPVAGQL 37 >SPBC19G7.04 |||HMG box protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 362 Score = 25.8 bits (54), Expect = 3.5 Identities = 15/43 (34%), Positives = 23/43 (53%), Gaps = 2/43 (4%) Frame = +3 Query: 291 KSKIMIPGKRGKKKRVKQID*NK--FSYVEIIDKVMVSKEXPY 413 KS G+ K+ K K ++Y+E+ DKV+V+KE Y Sbjct: 165 KSFSTTAGRANLKRNNKDAPLGKKTYAYIELSDKVIVTKERLY 207 >SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosaccharomyces pombe|chr 2|||Manual Length = 2335 Score = 25.4 bits (53), Expect = 4.6 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +1 Query: 307 YPGNGGKRKESNKLTEINLVMLR*LIKLWFQKKXHI 414 Y GN +++ES +L ++ + R LIK + Q H+ Sbjct: 615 YSGNSRQKEESAQLLKLLVSKARTLIKPYIQSIIHV 650 >SPAC19G12.14 |its3||1-phosphatidylinositol-4-phosphate 5-kinase Its3|Schizosaccharomyces pombe|chr 1|||Manual Length = 742 Score = 25.4 bits (53), Expect = 4.6 Identities = 9/29 (31%), Positives = 16/29 (55%) Frame = +1 Query: 88 RQHTGSKPYSCQFCSMEFTNWSNYNKHMK 174 R+ ++P C+M+ TNW N H++ Sbjct: 453 RELDENQPCQSPMCTMKDTNWIRRNMHLQ 481 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 25.4 bits (53), Expect = 4.6 Identities = 13/30 (43%), Positives = 21/30 (70%) Frame = +3 Query: 300 IMIPGKRGKKKRVKQID*NKFSYVEIIDKV 389 +++PG GKKK + +++ K SY EII +V Sbjct: 122 MIVPG--GKKKDMSKVEEYKESYEEIIKEV 149 >SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizosaccharomyces pombe|chr 2|||Manual Length = 953 Score = 25.0 bits (52), Expect = 6.1 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = -1 Query: 335 SFLFPPFPGYHDLTLPLLST 276 SF FPPFP Y D ++T Sbjct: 286 SFSFPPFPRYSDRMKRFIAT 305 >SPAC13F5.03c |||glycerol dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 25.0 bits (52), Expect = 6.1 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = -2 Query: 322 PRFPGIMILLFHSSVPAGSSTCTSVQ*WGQQEKRPLE 212 PRFP + + +S V A +S+ Q WG++ P+E Sbjct: 11 PRFPLVSLAHRNSKVFALASSNAVAQRWGKRFYAPIE 47 >SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase Cho2|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 24.6 bits (51), Expect = 8.1 Identities = 8/21 (38%), Positives = 14/21 (66%) Frame = +1 Query: 160 NKHMKRRHGTDTAKKKITPEG 222 N H++R +GTD +++P G Sbjct: 336 NPHIERTYGTDINHARLSPRG 356 >SPBC577.06c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1877 Score = 24.6 bits (51), Expect = 8.1 Identities = 9/26 (34%), Positives = 17/26 (65%) Frame = -2 Query: 397 ETITLSIIST*LNLFQSICLTLFFFP 320 + +TL +I+ N+F S+ L ++ FP Sbjct: 1632 DVLTLQLIAMFKNIFNSVGLDVYLFP 1657 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,025,900 Number of Sequences: 5004 Number of extensions: 42606 Number of successful extensions: 154 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 152 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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