BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0408
(403 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces... 124 4e-30
SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase Ubp12|Schizo... 25 4.4
>SPBC16G5.14c |rps3||40S ribosomal protein S3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 249
Score = 124 bits (300), Expect = 4e-30
Identities = 73/125 (58%), Positives = 87/125 (69%), Gaps = 1/125 (0%)
Frame = +2
Query: 20 AVNNISKKRKFVGDGVFKAELN*FLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLG 199
A ISKKRKFV DGVF AELN F TREL+E+GYSG EVRVTP RSEIII AT TQ VLG
Sbjct: 3 AAFTISKKRKFVADGVFYAELNEFFTRELSEEGYSGCEVRVTPSRSEIIIRATHTQDVLG 62
Query: 200 EKGRRIRELTSVVQKRFNIQSNL*NCMLKR-WLLVVFALSPRPNL*DTSLSEVSLYRRAC 376
EKGRRIRELT++VQKRF N ++ + A++ +L L+ +++ RRA
Sbjct: 63 EKGRRIRELTALVQKRFKFAENTVELYAEKVQNRGLCAVAQCESLRYKLLAGLAV-RRAA 121
Query: 377 YGVLR 391
YGVLR
Sbjct: 122 YGVLR 126
Score = 60.9 bits (141), Expect = 7e-11
Identities = 29/48 (60%), Positives = 34/48 (70%)
Frame = +1
Query: 259 EQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVPSCLLWCSPFIME 402
E +VELYAEKV RGLCA+AQ ESLRYKL+ GLAV ++ME
Sbjct: 83 ENTVELYAEKVQNRGLCAVAQCESLRYKLLAGLAVRRAAYGVLRYVME 130
>SPCC1494.05c |ubp12||ubiquitin C-terminal hydrolase
Ubp12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 979
Score = 25.0 bits (52), Expect = 4.4
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 7/71 (9%)
Frame = -2
Query: 345 KLVS*RFGLGDSAKTTSSHLFSIQFYRLLWMLNRFCTTEVSS-------RILRPFSPSTL 187
K V+ + G D K + + +FYR L L++ E+S + RP+ +
Sbjct: 533 KYVAEKSGCSDYRKILVTETYKGRFYRFLTQLSKSLLMEISEEDEIYLYELERPYEDGSD 592
Query: 186 CVLVAIIMISE 154
+LV + IS+
Sbjct: 593 DILVPVYHISD 603
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,660,862
Number of Sequences: 5004
Number of extensions: 30667
Number of successful extensions: 89
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 88
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 136158338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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