BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0402 (447 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 60 2e-10 SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc... 48 5e-07 SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac... 31 0.081 SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 29 0.43 SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy... 28 0.75 SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pomb... 27 0.99 SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schi... 27 1.3 SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 27 1.7 SPBC3B9.03 |||signal recognition particle receptor alpha subunit... 27 1.7 SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 26 2.3 SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 25 4.0 SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At... 25 5.3 SPBC1773.16c |||transcription factor |Schizosaccharomyces pombe|... 25 5.3 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 25 7.0 SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces... 25 7.0 SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|c... 24 9.3 SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosacch... 24 9.3 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 59.7 bits (138), Expect = 2e-10 Identities = 28/58 (48%), Positives = 37/58 (63%), Gaps = 1/58 (1%) Frame = +1 Query: 232 KTEVDRRRHAVVTVRDAK-HTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402 K + R H +++ R+ K NF+E+ N K+VYRRYA L FC CVD DN+L LE I Sbjct: 28 KVRLKARIHQLISQRNQKFQANFLEWENSKLVYRRYAGLYFCFCVDSTDNDLAILEMI 85 Score = 50.0 bits (114), Expect = 2e-07 Identities = 22/31 (70%), Positives = 27/31 (87%) Frame = +2 Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKL 244 MI+FILIQNR GK RL+K+Y+ FDDDEK +L Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDDDEKVRL 31 >SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 48.4 bits (110), Expect = 5e-07 Identities = 24/57 (42%), Positives = 34/57 (59%) Frame = +1 Query: 232 KTEVDRRRHAVVTVRDAKHTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402 + ++ R ++V R K NFVE++ KIVYRRYA L F ++ +DN L LE I Sbjct: 29 RAKIIRDVSSLVITRKPKMCNFVEYKGEKIVYRRYASLFFVCGIEQDDNELIILEVI 85 Score = 35.1 bits (77), Expect = 0.005 Identities = 14/33 (42%), Positives = 21/33 (63%) Frame = +2 Query: 155 IRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEE 253 I+F L+ +R GK RLAKW+ E+ K+I + Sbjct: 3 IKFFLLVSRQGKVRLAKWFNTLSIKERAKIIRD 35 >SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 165 Score = 31.1 bits (67), Expect = 0.081 Identities = 15/32 (46%), Positives = 19/32 (59%) Frame = +2 Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLI 247 MI + I N GK RL K+Y D+ +QKLI Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPIDESIQQKLI 32 Score = 31.1 bits (67), Expect = 0.081 Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 4/53 (7%) Frame = +1 Query: 256 HAVVTVRDAKHTNFVEFRNF----KIVYRRYAXLVFCICVDVNDNNLXYLEAI 402 +A V+ R NF+E +I+YR+YA L F VD ++ L L+ I Sbjct: 36 YAAVSTRPPTACNFLESNLIAGKNRIIYRQYATLYFVFVVDEGESELGILDLI 88 >SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 28.7 bits (61), Expect = 0.43 Identities = 15/54 (27%), Positives = 29/54 (53%) Frame = -2 Query: 326 YTILKFLNSTKFVCFASLTVTTACLLLSTSVFRHHQNSYTISQVESCLLGSGSK 165 + + ++ F S +VTTAC++L T FR H+ +I +++S ++ K Sbjct: 21 FKVPSWITYKSFWIAVSSSVTTACVILGTLEFRKHR---SIRRLQSMIVPEAGK 71 >SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 385 Score = 27.9 bits (59), Expect = 0.75 Identities = 22/83 (26%), Positives = 29/83 (34%) Frame = -1 Query: 429 YHLGPRQSYDGLEITQIVIIXIDTYTEDKTCIPPVYYLEISELDKVRVFRVSDGDDCMPX 250 Y + P Q DG+ + + D +E C P Y E+S +K F D P Sbjct: 158 YQVIPTQICDGVRGLEYAVYNYDLPSESTFCHPSNGYTEVSTFNKPAYFGSKDLKQSAPL 217 Query: 249 XXXXXXXXXXXFIYHFASRVLPA 181 Y AS LPA Sbjct: 218 FTGIFSSLDDIPTYS-ASDYLPA 239 >SPBC428.19c |||U3 snoRNP protein Utp15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 494 Score = 27.5 bits (58), Expect = 0.99 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +1 Query: 91 GGVNPSLKVIFKKKAITKRENDKIHFD 171 GGV + V+ KKK I K END+ + + Sbjct: 334 GGVGSAFGVVGKKKQIYKGENDEFYVE 360 >SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 27.1 bits (57), Expect = 1.3 Identities = 10/28 (35%), Positives = 17/28 (60%) Frame = +2 Query: 200 AKWYMNFDDDEKQKLIEEGMQSSPSETR 283 ++WY+ FD D+ +L+E + SE R Sbjct: 178 SRWYLPFDKDDDNQLVEAYAKYFSSENR 205 >SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 26.6 bits (56), Expect = 1.7 Identities = 14/44 (31%), Positives = 22/44 (50%), Gaps = 1/44 (2%) Frame = +2 Query: 161 FILIQNRAGKTRLAKWYM-NFDDDEKQKLIEEGMQSSPSETRNT 289 F +QNR+G+ L +W+ F D Q + + + S S NT Sbjct: 21 FFQLQNRSGQQDLIEWFREKFGKDISQPSVSQILSSKYSYLDNT 64 >SPBC3B9.03 |||signal recognition particle receptor alpha subunit Srp101|Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 26.6 bits (56), Expect = 1.7 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = +2 Query: 179 RAGKTRLAKWYMNFDDDEKQKLIEEGMQSSPSETRNTRTLSS 304 + GK +L KW ++E+ L SS S+T + LSS Sbjct: 165 KKGKKQLRKWDDQITEEEQAALNYSSQASSASQTVDNSQLSS 206 >SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1183 Score = 26.2 bits (55), Expect = 2.3 Identities = 8/25 (32%), Positives = 16/25 (64%) Frame = +1 Query: 142 KRENDKIHFDPEPSRQDSTCEMVYE 216 + + ++H +PEP QDS E++ + Sbjct: 35 QESSSEVHANPEPDNQDSNAEILID 59 >SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 710 Score = 25.4 bits (53), Expect = 4.0 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 230 EKQKLIEEGMQSSPSETRNTRTLSSSEI 313 EK IE+ ++ S T+N T+SSS + Sbjct: 328 EKSFTIEQNRENEKSSTKNDSTVSSSAV 355 >SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 758 Score = 25.0 bits (52), Expect = 5.3 Identities = 13/26 (50%), Positives = 17/26 (65%), Gaps = 3/26 (11%) Frame = -1 Query: 336 IPPVY---YLEISELDKVRVFRVSDG 268 IPP+ YL+IS+L K + VSDG Sbjct: 106 IPPMVLHTYLDISDLSKNQTLSVSDG 131 >SPBC1773.16c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 595 Score = 25.0 bits (52), Expect = 5.3 Identities = 10/30 (33%), Positives = 17/30 (56%) Frame = +3 Query: 279 RETHELCRVQKFQDSIQEVCXSCLLYMCRC 368 R ELCR +K + + + C +C++Y C Sbjct: 5 RNACELCRRKKLRCNGELTCQNCMVYGEEC 34 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 24.6 bits (51), Expect = 7.0 Identities = 10/35 (28%), Positives = 20/35 (57%) Frame = +2 Query: 146 VKMIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIE 250 +K +R + + R + + NF DD+KQK+++ Sbjct: 1233 LKSLRHLPLSQRILDANVTRLPSNFTDDKKQKIMK 1267 >SPBP8B7.30c |thi5||transcription factor Thi5|Schizosaccharomyces pombe|chr 2|||Manual Length = 857 Score = 24.6 bits (51), Expect = 7.0 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = +3 Query: 261 SRHRQRRETHELCRVQKFQDSIQEVCXSCLLYMCRC 368 SR R RR CR +K + S E C +C+ +C Sbjct: 30 SRRRVRRACLS-CRAKKIRCSGSEPCQACIATPSQC 64 >SPAC105.03c |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 708 Score = 24.2 bits (50), Expect = 9.3 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = +1 Query: 283 KHTNFVEFRNFKIVYRRYAXL 345 KH +++FRNF +V+ Y L Sbjct: 654 KHCVWLKFRNFDLVFMAYKRL 674 >SPAC22F3.08c |rok1||ATP-dependent RNA helicase Rok1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 24.2 bits (50), Expect = 9.3 Identities = 15/36 (41%), Positives = 18/36 (50%) Frame = +2 Query: 179 RAGKTRLAKWYMNFDDDEKQKLIEEGMQSSPSETRN 286 RAG T A + +D E KLI M+SS E N Sbjct: 381 RAGNTGQAVTFFTKEDGEYIKLIAGVMRSSGCEVPN 416 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,726,205 Number of Sequences: 5004 Number of extensions: 32479 Number of successful extensions: 132 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 164204010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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