Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0402
(447 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g47830.1 68414.m05324 clathrin coat assembly protein, putativ... 75 2e-14
At4g35410.2 68417.m05030 clathrin adaptor complex small chain fa... 61 4e-10
At4g35410.1 68417.m05029 clathrin adaptor complex small chain fa... 61 4e-10
At2g17380.1 68415.m02007 clathrin assembly protein AP19 identica... 60 9e-10
At2g19790.1 68415.m02312 clathrin adaptor complex small chain fa... 48 2e-06
At5g50375.1 68418.m06239 cyclopropyl isomerase (CPI1) 33 0.088
At5g55650.1 68418.m06939 hypothetical protein 31 0.47
At1g74680.1 68414.m08648 exostosin family protein contains Pfam ... 31 0.47
At3g50860.1 68416.m05569 clathrin adaptor complex small chain fa... 30 0.82
At3g50430.1 68416.m05516 expressed protein 29 1.1
At1g73100.1 68414.m08452 SET domain-containing protein (SUVH3) i... 29 1.9
At4g07460.1 68417.m01148 hypothetical protein 27 4.4
At1g74830.1 68414.m08670 expressed protein contains Pfam profile... 27 4.4
At5g02980.1 68418.m00241 kelch repeat-containing F-box family pr... 27 5.8
At1g79560.1 68414.m09275 FtsH protease, putative contains simila... 27 5.8
At5g47850.1 68418.m05912 protein kinase, putative contains simil... 27 7.6
At1g18990.1 68414.m02362 expressed protein contains Pfam profile... 27 7.6
>At1g47830.1 68414.m05324 clathrin coat assembly protein, putative
similar to clathrin coat assembly protein AP17
GB:CAA65533 GI:2959358 from [Zea mays]; contains Pfam
profile: PF01217 clathrin adaptor complex small chain
Length = 142
Score = 74.9 bits (176), Expect = 2e-14
Identities = 33/57 (57%), Positives = 41/57 (71%)
Frame = +1
Query: 232 KTEVDRRRHAVVTVRDAKHTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402
K +V+ H +V RDAK TNFVEFR K++YRRYA L F +CVD+ DN L YLE+I
Sbjct: 28 KHKVEYEVHRLVVNRDAKFTNFVEFRTHKVIYRRYAGLFFSVCVDITDNELAYLESI 84
Score = 47.2 bits (107), Expect = 5e-06
Identities = 20/31 (64%), Positives = 26/31 (83%)
Frame = +2
Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKL 244
MIRFIL+QNR GKTRLAK+Y+ ++ EK K+
Sbjct: 1 MIRFILLQNRQGKTRLAKYYVPLEESEKHKV 31
>At4g35410.2 68417.m05030 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 162
Score = 60.9 bits (141), Expect = 4e-10
Identities = 27/57 (47%), Positives = 37/57 (64%)
Frame = +1
Query: 232 KTEVDRRRHAVVTVRDAKHTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402
+++V R V+ R K NFVE+R +K+VY+RYA L FC+C+D DN L LE I
Sbjct: 28 RSKVIRELSGVILNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEII 84
Score = 39.9 bits (89), Expect = 8e-04
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEE 253
MI F+L+ +R GK RL KWY + E+ K+I E
Sbjct: 1 MIHFVLLVSRQGKVRLTKWYSPYAQKERSKVIRE 34
>At4g35410.1 68417.m05029 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 124
Score = 60.9 bits (141), Expect = 4e-10
Identities = 27/57 (47%), Positives = 37/57 (64%)
Frame = +1
Query: 232 KTEVDRRRHAVVTVRDAKHTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402
+++V R V+ R K NFVE+R +K+VY+RYA L FC+C+D DN L LE I
Sbjct: 28 RSKVIRELSGVILNRGPKLCNFVEWRGYKVVYKRYASLYFCMCIDQEDNELEVLEII 84
Score = 39.9 bits (89), Expect = 8e-04
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEE 253
MI F+L+ +R GK RL KWY + E+ K+I E
Sbjct: 1 MIHFVLLVSRQGKVRLTKWYSPYAQKERSKVIRE 34
>At2g17380.1 68415.m02007 clathrin assembly protein AP19 identical
to clathrin assembly protein AP19 GI:2231698 from
[Arabidopsis thaliana]
Length = 161
Score = 59.7 bits (138), Expect = 9e-10
Identities = 26/57 (45%), Positives = 37/57 (64%)
Frame = +1
Query: 232 KTEVDRRRHAVVTVRDAKHTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402
+++V R V+ R K NF+E+R +K+VY+RYA L FC+C+D DN L LE I
Sbjct: 28 RSKVIRELSGVILNRGPKLCNFIEWRGYKVVYKRYASLYFCMCIDEADNELEVLEII 84
Score = 40.3 bits (90), Expect = 6e-04
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEE 253
MI F+L+ +R GK RL KWY + E+ K+I E
Sbjct: 1 MIHFVLLVSRQGKVRLTKWYSPYTQKERSKVIRE 34
>At2g19790.1 68415.m02312 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 143
Score = 48.4 bits (110), Expect = 2e-06
Identities = 23/43 (53%), Positives = 30/43 (69%)
Frame = +1
Query: 274 RDAKHTNFVEFRNFKIVYRRYAXLVFCICVDVNDNNLXYLEAI 402
R+ + +FVE RN+KIVYRRYA L F + VD ++N L LE I
Sbjct: 43 RNDQQCSFVEHRNYKIVYRRYASLFFMVGVDDDENELAILEFI 85
Score = 30.7 bits (66), Expect = 0.47
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = +2
Query: 155 IRFILIQNRAGKTRLAKWYMNFDDDEKQKL 244
IRFIL+ N+ G+TRLA++Y +E++ L
Sbjct: 3 IRFILMVNKQGQTRLAQYYEWLTLEERRAL 32
>At5g50375.1 68418.m06239 cyclopropyl isomerase (CPI1)
Length = 280
Score = 33.1 bits (72), Expect = 0.088
Identities = 14/33 (42%), Positives = 22/33 (66%), Gaps = 2/33 (6%)
Frame = -1
Query: 180 RFWIKMNLIIFTFSY--GFFFKYYFQRRVDASF 88
R+W+K NL I FSY +F+ +YF + + AS+
Sbjct: 84 RYWVKANLWIIVFSYVGNYFWTHYFFKVLGASY 116
>At5g55650.1 68418.m06939 hypothetical protein
Length = 154
Score = 30.7 bits (66), Expect = 0.47
Identities = 16/40 (40%), Positives = 26/40 (65%), Gaps = 6/40 (15%)
Frame = -2
Query: 332 LLYTILKFLNSTKFVCF------ASLTVTTACLLLSTSVF 231
LL+ ++ ++S F+CF +SLT+T A L +S+SVF
Sbjct: 22 LLFFLVTLISSVAFLCFLNSQGVSSLTITMAILFVSSSVF 61
>At1g74680.1 68414.m08648 exostosin family protein contains Pfam
profile: PF03016 Exostosin family
Length = 461
Score = 30.7 bits (66), Expect = 0.47
Identities = 18/46 (39%), Positives = 30/46 (65%), Gaps = 1/46 (2%)
Frame = -2
Query: 314 KFLNSTKFVCFASLTVTTACLLLSTSVF-RHHQNSYTISQVESCLL 180
K L S+KF+ F ++TV+T ++S+ VF + H +S+T S V +L
Sbjct: 7 KSLLSSKFL-FYTITVSTLLFIVSSLVFLQRHDSSFTSSLVRKLIL 51
>At3g50860.1 68416.m05569 clathrin adaptor complex small chain
family protein contains Pfam profile: PF01217 clathrin
adaptor complex small chain
Length = 166
Score = 29.9 bits (64), Expect = 0.82
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +2
Query: 152 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLI 247
MI+ +++ N GK RLAK+Y +++Q+LI
Sbjct: 1 MIKAVMMMNTQGKPRLAKFYDYLPVEKQQELI 32
>At3g50430.1 68416.m05516 expressed protein
Length = 642
Score = 29.5 bits (63), Expect = 1.1
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = -2
Query: 305 NSTKFVCFASLTVTTACLLLSTSVFRHHQNSYTISQVESCLLGSGSK 165
+S +++C L CLL +V H + +V CL+ SGS+
Sbjct: 83 DSIEYLCLERLVADLVCLLGMENVHVKHLAGNILVEVSGCLVESGSQ 129
>At1g73100.1 68414.m08452 SET domain-containing protein (SUVH3)
identical to SUVH3 [Arabidopsis thaliana] GI:13517747;
contains Pfam profiles PF00856: SET domain, PF05033:
Pre-SET motif, PF02182: YDG/SRA domain; identical to
cDNA SUVH3 (SUVH3) GI:14625477
Length = 669
Score = 28.7 bits (61), Expect = 1.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +1
Query: 352 CICVDVNDNNLXYLEAIITLSRS*MIF 432
C C+ ND +L YL +I +SR +I+
Sbjct: 445 CSCIRKNDGDLPYLNGVILVSRRPVIY 471
>At4g07460.1 68417.m01148 hypothetical protein
Length = 205
Score = 27.5 bits (58), Expect = 4.4
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -1
Query: 159 LIIFTFSYGFFFKYYFQR 106
LI+F+FS+ FF +YF R
Sbjct: 184 LILFSFSFPFFLFFYFSR 201
>At1g74830.1 68414.m08670 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 542
Score = 27.5 bits (58), Expect = 4.4
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = +3
Query: 258 CSRHRQRRETHELCRVQKFQDSIQEVCXSCLL 353
C H+++ + C V K I+ +C CLL
Sbjct: 100 CDSHKKKVSSLAYCHVHKKLSEIKHMCEGCLL 131
>At5g02980.1 68418.m00241 kelch repeat-containing F-box family
protein contains F-box domain Pfam:PF00646 and Kelch
motif Pfam:PF01344
Length = 335
Score = 27.1 bits (57), Expect = 5.8
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = -2
Query: 377 LSXTSTHIQKT-RXAYLLYTILKFLNSTKFVCFASLTVTTACLLLSTSVFRHHQNSYTIS 201
L T + I KT R Y+ + K ++ K+ F V LL +F +H NS T+S
Sbjct: 53 LYATRSRIGKTERFLYICLNLTK--SNPKYRWFTLPPVPNEQKLLPVPLFTYHLNSSTVS 110
Query: 200 QVES 189
+S
Sbjct: 111 STDS 114
>At1g79560.1 68414.m09275 FtsH protease, putative contains
similarity to chloroplast FtsH protease GI:5804782 from
[Nicotiana tabacum]
Length = 1008
Score = 27.1 bits (57), Expect = 5.8
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +2
Query: 200 AKWYMNFDDDEKQKLIEEGMQSSPSETRNTRTL 298
AK+ + FD E +K + E + SET TR L
Sbjct: 315 AKYVVEFDMKEVEKSLREDVIGRTSETEGTRAL 347
>At5g47850.1 68418.m05912 protein kinase, putative contains
similarity to cytokinin-regulated kinase 1 [Nicotiana
tabacum] gi|10998537|gb|AAG25966; contains protein
kinase domain, Pfam:PF00069
Length = 751
Score = 26.6 bits (56), Expect = 7.6
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = -2
Query: 338 AYLLYTILKFLNSTKFVCFASLTVTTACLLLSTSVFRHHQNSY 210
+Y + +L L+S FVCF+ TV+ + + T V + +SY
Sbjct: 12 SYFVSLLLLVLSSFSFVCFSLSTVSISHISNQTLVCALNNHSY 54
>At1g18990.1 68414.m02362 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593
Length = 524
Score = 26.6 bits (56), Expect = 7.6
Identities = 10/32 (31%), Positives = 15/32 (46%)
Frame = +3
Query: 258 CSRHRQRRETHELCRVQKFQDSIQEVCXSCLL 353
C H++ + C V K I+ +C CLL
Sbjct: 81 CDAHKKNVSSLAYCHVHKKLSEIKRMCEGCLL 112
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,803,490
Number of Sequences: 28952
Number of extensions: 162181
Number of successful extensions: 427
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 410
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 426
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 722638680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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