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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0291
         (560 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote...    35   0.032
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote...    34   0.057
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS...    34   0.057
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo...    33   0.099
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS...    32   0.23 
At4g03620.1 68417.m00497 myosin heavy chain-related contains wea...    29   2.1  
At3g62110.1 68416.m06978 glycoside hydrolase family 28 protein /...    29   2.1  
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)...    28   4.9  
At1g20410.1 68414.m02545 expressed protein                             28   4.9  
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase...    27   6.5  
At1g19485.1 68414.m02427 AT hook motif-containing protein contai...    27   6.5  
At2g47410.1 68415.m05917 transducin family protein / WD-40 repea...    27   8.6  
At1g20740.1 68414.m02598 expressed protein ; expression supporte...    27   8.6  

>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
           (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
           crystallin family; identified in Scharf, K-D., et al,
           Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 153

 Score = 35.1 bits (77), Expect = 0.032
 Identities = 25/67 (37%), Positives = 39/67 (58%), Gaps = 8/67 (11%)
 Frame = +2

Query: 296 EEISVKTADGYVI-VEG-KHEERQDEHGYIXR------QFTRRSALPENCNPDTVESRLS 451
           EE+ V+  +  V+ + G +H E++D++    R      QFTRR  LPEN   D V++ + 
Sbjct: 69  EEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAME 128

Query: 452 YDGVLTV 472
            +GVLTV
Sbjct: 129 -NGVLTV 134


>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
           (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
           heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
           (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
           17 (19), 7995 (1989))
          Length = 157

 Score = 34.3 bits (75), Expect = 0.057
 Identities = 22/67 (32%), Positives = 40/67 (59%), Gaps = 8/67 (11%)
 Frame = +2

Query: 296 EEISVKTADGYVI-VEGK----HEERQDEHGYIXR---QFTRRSALPENCNPDTVESRLS 451
           EE+ V+  DG ++ + G+    +EE+ D+   + R   +FTRR  LPEN   + +++ + 
Sbjct: 73  EEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEIKASME 132

Query: 452 YDGVLTV 472
            +GVL+V
Sbjct: 133 -NGVLSV 138


>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
           (HSP17.8-CI) similar to 17.5 kDa class I heat shock
           protein SP:P04793 from [Glycine max]; contains Pfam
           PF00011: Hsp20/alpha crystallin family
          Length = 157

 Score = 34.3 bits (75), Expect = 0.057
 Identities = 25/67 (37%), Positives = 38/67 (56%), Gaps = 8/67 (11%)
 Frame = +2

Query: 296 EEISVKTADGYVI-VEGKH----EERQDEHGYIXR---QFTRRSALPENCNPDTVESRLS 451
           EE+ V+  D  V+ + G+     EE+QD    + R   QF+R+  LPEN   D V++ + 
Sbjct: 71  EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASME 130

Query: 452 YDGVLTV 472
            +GVLTV
Sbjct: 131 -NGVLTV 136


>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
           chloroplast precursor (HSP25.3-P) identical to small
           heat shock protein, chloroplast precursor SP:P31170 from
           [Arabidopsis thaliana]; identified in Scharf, K-D., et
           al,Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 227

 Score = 33.5 bits (73), Expect = 0.099
 Identities = 15/65 (23%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
 Frame = +2

Query: 287 LFAEEISVKTADGYVIVEGKHEERQDEHGYIXRQ---FTRRSALPENCNPDTVESRLSYD 457
           L  E++ +   D  ++++G+ ++   +  +  R    +  R  LP+NC  D +++ L  +
Sbjct: 147 LSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDKIKAELK-N 205

Query: 458 GVLTV 472
           GVL +
Sbjct: 206 GVLFI 210


>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
           (HSP17.6A-CI) similar to 17.5 kDa class I heat shock
           protein SP:P04793 from [Glycine max]
          Length = 155

 Score = 32.3 bits (70), Expect = 0.23
 Identities = 24/67 (35%), Positives = 37/67 (55%), Gaps = 8/67 (11%)
 Frame = +2

Query: 296 EEISVKTADGYVI-VEGKH----EERQDEHGYIXRQ---FTRRSALPENCNPDTVESRLS 451
           EE+ V+  D  V+ + G+     EE+QD    + R    F+R+  LPEN   D V++ + 
Sbjct: 69  EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKASME 128

Query: 452 YDGVLTV 472
            +GVLTV
Sbjct: 129 -NGVLTV 134


>At4g03620.1 68417.m00497 myosin heavy chain-related contains weak
           similarity to Swiss-Prot:P24733 myosin heavy chain,
           striated muscle [Aequipecten irradians]
          Length = 342

 Score = 29.1 bits (62), Expect = 2.1
 Identities = 16/49 (32%), Positives = 30/49 (61%)
 Frame = +2

Query: 239 PSPRIRQIPGQLXRSTLFAEEISVKTADGYVIVEGKHEERQDEHGYIXR 385
           P P    +P Q  R+   +E++ +K+ DGY  ++ K+++ QDE+G + R
Sbjct: 240 PGPGDNNVPLQ-GRNKELSEQL-LKSIDGYRSLKRKYKDVQDENGSMRR 286


>At3g62110.1 68416.m06978 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to polygalacturonase [Lycopersicon
           esculentum] GI:4325090; contains PF00295: Glycosyl
           hydrolases family 28
          Length = 471

 Score = 29.1 bits (62), Expect = 2.1
 Identities = 11/38 (28%), Positives = 21/38 (55%)
 Frame = +2

Query: 194 GRGSRWRWPIETSVPPSPRIRQIPGQLXRSTLFAEEIS 307
           G  S   WP+   +P   R R++PG+  RS ++ + ++
Sbjct: 110 GSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLT 147


>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
           nearly identical to SH3 domain-containing protein 1
           [Arabidopsis thaliana] GI:16974676; contains Pfam
           profile PF00018: SH3 domain
          Length = 439

 Score = 27.9 bits (59), Expect = 4.9
 Identities = 16/38 (42%), Positives = 17/38 (44%)
 Frame = +3

Query: 120 LRTGAYSGRSPDGRHHANDVQRLLPAVEADGDGQ*RRR 233
           +RT  YS    D RH  N   RL   VEA      RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173


>At1g20410.1 68414.m02545 expressed protein
          Length = 504

 Score = 27.9 bits (59), Expect = 4.9
 Identities = 17/51 (33%), Positives = 28/51 (54%)
 Frame = -3

Query: 162 DGRQEIVRSKRQSEVLIAKAVRLVSQDEWQQRHFYFTLGCEISSDITVRSN 10
           D ++E+V+S   S+  +A+   LV QD    RH + + G E+S   T+  N
Sbjct: 111 DVKKELVKSDSSSDY-VARITDLVKQD----RHEFDSFGLEVSVPSTIMEN 156


>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
           large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
           identical to SP|P55230
          Length = 518

 Score = 27.5 bits (58), Expect = 6.5
 Identities = 16/65 (24%), Positives = 30/65 (46%)
 Frame = +2

Query: 296 EEISVKTADGYVIVEGKHEERQDEHGYIXRQFTRRSALPENCNPDTVESRLSYDGVLTVI 475
           E+   K  +  +I+ G H  R D   ++ +     + +  +C P   ESR S  G+L + 
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKID 256

Query: 476 XSQEL 490
            S ++
Sbjct: 257 QSGKI 261


>At1g19485.1 68414.m02427 AT hook motif-containing protein contains
           Pfam profile: PF00730 HhH-GPD superfamily base excision
           DNA repair protein; contains Pfam PF02178: AT hook
           motif; contains Pfam PF00400: WD domain, G-beta repeat
           (5 copies); contains Prosite PS00354: HMG-I and HMG-Y
           DNA-binding domain (A+T-hook)
          Length = 815

 Score = 27.5 bits (58), Expect = 6.5
 Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 7/53 (13%)
 Frame = +2

Query: 197 RGSRWRWPIETSVPPSPRIR-------QIPGQLXRSTLFAEEISVKTADGYVI 334
           RG   + P+ET+ P  PR R       ++P +L    L+ E +SV+  +  V+
Sbjct: 212 RGRPRKHPVETTEPKKPRGRPRKKSTAELPVELDDDVLYVEALSVRYPENSVV 264


>At2g47410.1 68415.m05917 transducin family protein / WD-40 repeat
           family protein contains 5 WD-40 repeats (PF00400);
           similar to WDR protein, form B (GI:14970593) [Mus
           musculus]
          Length = 1589

 Score = 27.1 bits (57), Expect = 8.6
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -2

Query: 265 WNLSYSR*WWNRRLYWPSPSASTAGS 188
           W+  YS+  W  R+Y PSPS +  GS
Sbjct: 419 WDARYSQ--WLPRIYVPSPSDANTGS 442


>At1g20740.1 68414.m02598 expressed protein ; expression supported
           by MPSS
          Length = 266

 Score = 27.1 bits (57), Expect = 8.6
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = -3

Query: 135 KRQSEVLIAKAVRLVSQDEWQQRHFYFTL 49
           KR  EVL  +  R +  D+W    F FT+
Sbjct: 237 KRTKEVLFFERYREIFNDDWDDHDFAFTI 265


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,463,845
Number of Sequences: 28952
Number of extensions: 194189
Number of successful extensions: 505
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 495
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 505
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1072696904
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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