BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0291 (560 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 35 0.032 At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 34 0.057 At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 34 0.057 At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 33 0.099 At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 32 0.23 At4g03620.1 68417.m00497 myosin heavy chain-related contains wea... 29 2.1 At3g62110.1 68416.m06978 glycoside hydrolase family 28 protein /... 29 2.1 At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 4.9 At1g20410.1 68414.m02545 expressed protein 28 4.9 At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 6.5 At1g19485.1 68414.m02427 AT hook motif-containing protein contai... 27 6.5 At2g47410.1 68415.m05917 transducin family protein / WD-40 repea... 27 8.6 At1g20740.1 68414.m02598 expressed protein ; expression supporte... 27 8.6 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 35.1 bits (77), Expect = 0.032 Identities = 25/67 (37%), Positives = 39/67 (58%), Gaps = 8/67 (11%) Frame = +2 Query: 296 EEISVKTADGYVI-VEG-KHEERQDEHGYIXR------QFTRRSALPENCNPDTVESRLS 451 EE+ V+ + V+ + G +H E++D++ R QFTRR LPEN D V++ + Sbjct: 69 EEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAME 128 Query: 452 YDGVLTV 472 +GVLTV Sbjct: 129 -NGVLTV 134 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 34.3 bits (75), Expect = 0.057 Identities = 22/67 (32%), Positives = 40/67 (59%), Gaps = 8/67 (11%) Frame = +2 Query: 296 EEISVKTADGYVI-VEGK----HEERQDEHGYIXR---QFTRRSALPENCNPDTVESRLS 451 EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR LPEN + +++ + Sbjct: 73 EEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEIKASME 132 Query: 452 YDGVLTV 472 +GVL+V Sbjct: 133 -NGVLSV 138 >At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HSP17.8-CI) similar to 17.5 kDa class I heat shock protein SP:P04793 from [Glycine max]; contains Pfam PF00011: Hsp20/alpha crystallin family Length = 157 Score = 34.3 bits (75), Expect = 0.057 Identities = 25/67 (37%), Positives = 38/67 (56%), Gaps = 8/67 (11%) Frame = +2 Query: 296 EEISVKTADGYVI-VEGKH----EERQDEHGYIXR---QFTRRSALPENCNPDTVESRLS 451 EE+ V+ D V+ + G+ EE+QD + R QF+R+ LPEN D V++ + Sbjct: 71 EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASME 130 Query: 452 YDGVLTV 472 +GVLTV Sbjct: 131 -NGVLTV 136 >At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chloroplast precursor (HSP25.3-P) identical to small heat shock protein, chloroplast precursor SP:P31170 from [Arabidopsis thaliana]; identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 227 Score = 33.5 bits (73), Expect = 0.099 Identities = 15/65 (23%), Positives = 34/65 (52%), Gaps = 3/65 (4%) Frame = +2 Query: 287 LFAEEISVKTADGYVIVEGKHEERQDEHGYIXRQ---FTRRSALPENCNPDTVESRLSYD 457 L E++ + D ++++G+ ++ + + R + R LP+NC D +++ L + Sbjct: 147 LSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDKIKAELK-N 205 Query: 458 GVLTV 472 GVL + Sbjct: 206 GVLFI 210 >At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HSP17.6A-CI) similar to 17.5 kDa class I heat shock protein SP:P04793 from [Glycine max] Length = 155 Score = 32.3 bits (70), Expect = 0.23 Identities = 24/67 (35%), Positives = 37/67 (55%), Gaps = 8/67 (11%) Frame = +2 Query: 296 EEISVKTADGYVI-VEGKH----EERQDEHGYIXRQ---FTRRSALPENCNPDTVESRLS 451 EE+ V+ D V+ + G+ EE+QD + R F+R+ LPEN D V++ + Sbjct: 69 EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKASME 128 Query: 452 YDGVLTV 472 +GVLTV Sbjct: 129 -NGVLTV 134 >At4g03620.1 68417.m00497 myosin heavy chain-related contains weak similarity to Swiss-Prot:P24733 myosin heavy chain, striated muscle [Aequipecten irradians] Length = 342 Score = 29.1 bits (62), Expect = 2.1 Identities = 16/49 (32%), Positives = 30/49 (61%) Frame = +2 Query: 239 PSPRIRQIPGQLXRSTLFAEEISVKTADGYVIVEGKHEERQDEHGYIXR 385 P P +P Q R+ +E++ +K+ DGY ++ K+++ QDE+G + R Sbjct: 240 PGPGDNNVPLQ-GRNKELSEQL-LKSIDGYRSLKRKYKDVQDENGSMRR 286 >At3g62110.1 68416.m06978 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein weak similarity to polygalacturonase [Lycopersicon esculentum] GI:4325090; contains PF00295: Glycosyl hydrolases family 28 Length = 471 Score = 29.1 bits (62), Expect = 2.1 Identities = 11/38 (28%), Positives = 21/38 (55%) Frame = +2 Query: 194 GRGSRWRWPIETSVPPSPRIRQIPGQLXRSTLFAEEIS 307 G S WP+ +P R R++PG+ RS ++ + ++ Sbjct: 110 GSTSSENWPVVDPLPSYGRGRELPGRRHRSLIYGQNLT 147 >At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1) nearly identical to SH3 domain-containing protein 1 [Arabidopsis thaliana] GI:16974676; contains Pfam profile PF00018: SH3 domain Length = 439 Score = 27.9 bits (59), Expect = 4.9 Identities = 16/38 (42%), Positives = 17/38 (44%) Frame = +3 Query: 120 LRTGAYSGRSPDGRHHANDVQRLLPAVEADGDGQ*RRR 233 +RT YS D RH N RL VEA RRR Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173 >At1g20410.1 68414.m02545 expressed protein Length = 504 Score = 27.9 bits (59), Expect = 4.9 Identities = 17/51 (33%), Positives = 28/51 (54%) Frame = -3 Query: 162 DGRQEIVRSKRQSEVLIAKAVRLVSQDEWQQRHFYFTLGCEISSDITVRSN 10 D ++E+V+S S+ +A+ LV QD RH + + G E+S T+ N Sbjct: 111 DVKKELVKSDSSSDY-VARITDLVKQD----RHEFDSFGLEVSVPSTIMEN 156 >At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase large subunit 2 (APL2) / ADP-glucose pyrophosphorylase identical to SP|P55230 Length = 518 Score = 27.5 bits (58), Expect = 6.5 Identities = 16/65 (24%), Positives = 30/65 (46%) Frame = +2 Query: 296 EEISVKTADGYVIVEGKHEERQDEHGYIXRQFTRRSALPENCNPDTVESRLSYDGVLTVI 475 E+ K + +I+ G H R D ++ + + + +C P ESR S G+L + Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKID 256 Query: 476 XSQEL 490 S ++ Sbjct: 257 QSGKI 261 >At1g19485.1 68414.m02427 AT hook motif-containing protein contains Pfam profile: PF00730 HhH-GPD superfamily base excision DNA repair protein; contains Pfam PF02178: AT hook motif; contains Pfam PF00400: WD domain, G-beta repeat (5 copies); contains Prosite PS00354: HMG-I and HMG-Y DNA-binding domain (A+T-hook) Length = 815 Score = 27.5 bits (58), Expect = 6.5 Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 7/53 (13%) Frame = +2 Query: 197 RGSRWRWPIETSVPPSPRIR-------QIPGQLXRSTLFAEEISVKTADGYVI 334 RG + P+ET+ P PR R ++P +L L+ E +SV+ + V+ Sbjct: 212 RGRPRKHPVETTEPKKPRGRPRKKSTAELPVELDDDVLYVEALSVRYPENSVV 264 >At2g47410.1 68415.m05917 transducin family protein / WD-40 repeat family protein contains 5 WD-40 repeats (PF00400); similar to WDR protein, form B (GI:14970593) [Mus musculus] Length = 1589 Score = 27.1 bits (57), Expect = 8.6 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -2 Query: 265 WNLSYSR*WWNRRLYWPSPSASTAGS 188 W+ YS+ W R+Y PSPS + GS Sbjct: 419 WDARYSQ--WLPRIYVPSPSDANTGS 442 >At1g20740.1 68414.m02598 expressed protein ; expression supported by MPSS Length = 266 Score = 27.1 bits (57), Expect = 8.6 Identities = 11/29 (37%), Positives = 15/29 (51%) Frame = -3 Query: 135 KRQSEVLIAKAVRLVSQDEWQQRHFYFTL 49 KR EVL + R + D+W F FT+ Sbjct: 237 KRTKEVLFFERYREIFNDDWDDHDFAFTI 265 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,463,845 Number of Sequences: 28952 Number of extensions: 194189 Number of successful extensions: 505 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 495 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 505 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1072696904 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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