Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0251
         (666 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g08415.1 68418.m00991 lipoic acid synthase family protein sim...   102   2e-22
At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical t...   102   2e-22
At5g16280.1 68418.m01901 expressed protein                             29   3.7  
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil...    29   3.7  
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam...    28   4.9  
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil...    28   4.9  
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam...    28   6.4  
At3g55160.1 68416.m06126 expressed protein                             27   8.5  

>At5g08415.1 68418.m00991 lipoic acid synthase family protein
           similar to lipoic acid synthase from Arabidopsis
           thaliana [gi:3928758], from Mus musculus [gi:14669826]
           Pfam profile PF04055: radical SAM domain protein
          Length = 394

 Score =  102 bits (245), Expect = 2e-22
 Identities = 50/80 (62%), Positives = 59/80 (73%)
 Frame = +2

Query: 14  DILVECLVPDFRAVRGCVARVASSGLDVFAHNVETVERLTPFVRDPRAGYRQTLKVLQMA 193
           DI++ECL  DFR     V  +  SGLDVFAHNVETV+RL   VRDPRAGY Q++ VL+ A
Sbjct: 227 DIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGYEQSMSVLKHA 286

Query: 194 KEINPDLITKSSIMLGLGET 253
           K   P +ITK+SIMLGLGET
Sbjct: 287 KISKPGMITKTSIMLGLGET 306



 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 22/38 (57%), Positives = 28/38 (73%)
 Frame = +1

Query: 259 QVEQTMKDLREAGVDCVTLGQYMQPTKKHLKVFEYVTP 372
           ++++ M DLR   VD +TLGQY+QPT  HL V EYVTP
Sbjct: 309 ELKEAMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTP 346


>At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical to
           gi:3928758 contains Pfam profile PF04055: radical SAM
           domain protein
          Length = 374

 Score =  102 bits (244), Expect = 2e-22
 Identities = 49/81 (60%), Positives = 62/81 (76%), Gaps = 1/81 (1%)
 Frame = +2

Query: 14  DILVECLVPDFRAVRGCVARVASSGLDVFAHNVETVERLTPFVRDPRAGYRQTLKVLQMA 193
           ++L+E LVPDFR   GCV +V+ SGLDV AHN+ETVE L  FVRD RA ++Q+L VL+MA
Sbjct: 203 EMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNIETVEELQSFVRDHRANFKQSLDVLRMA 262

Query: 194 KEINP-DLITKSSIMLGLGET 253
           KE  P   +TK+S+MLG GET
Sbjct: 263 KEYAPAGTLTKTSVMLGCGET 283



 Score = 54.8 bits (126), Expect = 5e-08
 Identities = 23/38 (60%), Positives = 31/38 (81%)
 Frame = +1

Query: 259 QVEQTMKDLREAGVDCVTLGQYMQPTKKHLKVFEYVTP 372
           QV +TM+ +R AGVD +T GQYM+P+K+H+ V EYVTP
Sbjct: 286 QVVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVAEYVTP 323



 Score = 27.5 bits (58), Expect = 8.5
 Identities = 10/22 (45%), Positives = 17/22 (77%)
 Frame = +2

Query: 434 LVRSSYRAGEFFISAXLRDKKV 499
           +VRSSY+AGE++I + +   +V
Sbjct: 346 MVRSSYKAGEYYIKSMIEADRV 367


>At5g16280.1 68418.m01901 expressed protein
          Length = 1265

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 13/43 (30%), Positives = 25/43 (58%)
 Frame = -1

Query: 252  VSPSPSMMDDFVIKSGFISLAICKTFNVWRYPALGSRTKGVSL 124
            V+PSPS + +F+++   ++ A    F + +   +G R  G+SL
Sbjct: 933  VTPSPSRLQEFLVRMDIVNRAKSDCFQIHQLSTVGCRW-GISL 974


>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
           to AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam AMP-binding domain PF00501; identical to
           cDNA acyl-activating enzyme 12 (At1g65890) mRNA
           GI:29893228, acyl-activating enzyme 12 [Arabidopsis
           thaliana] GI:29893229
          Length = 578

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = -1

Query: 528 KASLLPVLRVTFLSRSXALMKNSPAL*DERTRLRWPRT 415
           +A+ +P+  +TFL R+     N  ++   +TR  WP+T
Sbjct: 8   EANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQT 45


>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501
          Length = 535

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = -1

Query: 528 KASLLPVLRVTFLSRSXALMKNSPAL*DERTRLRWPRT 415
           +A+ +P+  +TFL R+     N  ++   +TR  WP+T
Sbjct: 8   EANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQT 45


>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
           to AMP-binding protein GI:1903034 from [Brassica napus];
           contains Pfam AMP-binding domain PF00501; identical to
           cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
           acyl-activating enzyme 11 [Arabidopsis thaliana]
           GI:29893231
          Length = 572

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = -1

Query: 528 KASLLPVLRVTFLSRSXALMKNSPAL*DERTRLRWPRT 415
           +A+ +P+  +TFL R+     N  ++   +TR  WP+T
Sbjct: 8   EANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQT 45


>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
           protein similar to AMP-binding protein GI:1903034 from
           [Brassica napus]; contains Pfam AMP-binding domain
           PF00501
          Length = 580

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = -1

Query: 528 KASLLPVLRVTFLSRSXALMKNSPAL*DERTRLRWPRT 415
           +A+ +P+  +TFL R+     N  ++   +TR  WP+T
Sbjct: 8   EANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQT 45


>At3g55160.1 68416.m06126 expressed protein
          Length = 2149

 Score = 27.5 bits (58), Expect = 8.5
 Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
 Frame = +2

Query: 62   CVARVASSGLD---VFAHNVETVERLTPFVRDPRAGYRQTLKVLQMAKEINPDLITKSSI 232
            C+ R ++   D   +F+  +E  E L PF+R+P       + V    K +N  L+  S++
Sbjct: 2002 CIFRFSTDSNDKKTLFSDIIELGEALKPFLRNPLVSNMFRVVVRLHEKLLNDSLMDLSTV 2061

Query: 233  MLG 241
            + G
Sbjct: 2062 LSG 2064


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,690,519
Number of Sequences: 28952
Number of extensions: 263894
Number of successful extensions: 721
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 700
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 720
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1403159472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)