Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0235
         (657 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr 2...    30   0.34 
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa...    27   2.4  
SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha su...    27   2.4  
SPBC30D10.10c |tor1||phosphatidylinositol kinase Tor1|Schizosacc...    26   4.2  
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa...    26   5.5  
SPAC1782.09c |clp1|flp1|Cdc14-related protein phosphatase Clp1/F...    26   5.5  

>SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 479

 Score = 29.9 bits (64), Expect = 0.34
 Identities = 28/95 (29%), Positives = 45/95 (47%), Gaps = 2/95 (2%)
 Frame = -2

Query: 356 TPVESSRTIVPHTRTRYPQMIQKKLTHQRSNISAA*TRRATL--AGPSLVESYKTKRDSS 183
           TP  + RT+ PH R    +  +  L  +RSN+ +A T R  L     +L ++       S
Sbjct: 36  TPRSAQRTVTPHKRRALAR--RNSLARRRSNVFSATTPRDILRMLSRALAKNPVPSPAES 93

Query: 182 E*TMRQCTKFTSHSLEILSKWRSARTTRRSQQNDA 78
           E + R+ T  T +S +     R +   RR+ +NDA
Sbjct: 94  ESSERRHTPQT-NSQKSQKTPRLSSNKRRTLKNDA 127


>SPCC550.03c |||RNA helicase involved in mRNA
           catabolism|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1213

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 10/15 (66%), Positives = 13/15 (86%)
 Frame = +1

Query: 316 RVCGTIVRLDSTGVR 360
           RVC TI+R+DS G+R
Sbjct: 908 RVCNTILRIDSGGIR 922


>SPAC458.07 |tfa1|SPAPYUG7.01|transcription factor TFIIE alpha
           subunit Tfa1 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 448

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 11/46 (23%), Positives = 21/46 (45%)
 Frame = -2

Query: 242 RATLAGPSLVESYKTKRDSSE*TMRQCTKFTSHSLEILSKWRSART 105
           R + + PS+   +   +++ E   R C K    +  IL +W +  T
Sbjct: 242 RLSTSSPSITVDFSADKETDEKRERNCDKQVKAAQNILPEWHATST 287


>SPBC30D10.10c |tor1||phosphatidylinositol kinase
            Tor1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2335

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 16/35 (45%), Positives = 20/35 (57%)
 Frame = +3

Query: 414  NDISKSVSLKVLGLCTIVSSNKKKYHHASFILPSI 518
            N  S+SVS KVL    I  SN ++Y H   +LP I
Sbjct: 941  NTSSRSVSYKVLQSFVIFGSNIEEYMH--LVLPVI 973


>SPAC1B9.02c |sck1||serine/threonine protein kinase
           Sck1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 696

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 26/104 (25%), Positives = 51/104 (49%), Gaps = 3/104 (2%)
 Frame = -1

Query: 348 RVQPNYRTAYPNS--ISSDDSKKTNASEIKHLGCMNTPRHACGSFPRRVLQN*T*FERIN 175
           +VQP ++    N   +S+ D + TN + +K++  ++    A  S P   L N T  +R  
Sbjct: 575 KVQPPFKPNVQNDLDVSNFDKEFTNTN-VKNINIVSNVDPANASTP---LSN-TIQDRFR 629

Query: 174 NAAVYKIYITFTRNIKQMAVRADDATEPAE*C-TTSHSSVHSLN 46
                 +  +     + + ++ ++ T+    C TT+HSSV+S+N
Sbjct: 630 GFTF--VNKSIDEQFQNLGLQENEETDNLHACRTTTHSSVNSIN 671


>SPAC1782.09c |clp1|flp1|Cdc14-related protein phosphatase
           Clp1/Flp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 537

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = -3

Query: 268 QTSRLHEHAAPRLRVLPSSSLTKLNVIRANKQCGSVQN 155
           Q  ++  H  P  R LPS+S  K N    N    S+QN
Sbjct: 391 QPRKVSGHNPPSARRLPSASSVKFNEKLKNASKQSIQN 428


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,357,732
Number of Sequences: 5004
Number of extensions: 43642
Number of successful extensions: 108
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 105
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 108
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)