Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0230
(660 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 121 1e-28
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 121 1e-28
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 121 1e-28
SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyce... 28 1.4
SPAC9.08c |||steroid reductase |Schizosaccharomyces pombe|chr 1|... 28 1.4
SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe... 26 5.5
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 5.5
SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual 25 7.3
SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein L13/L16|Schizos... 25 7.3
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 25 9.7
SPCC645.13 |||transcription elongation regulator|Schizosaccharom... 25 9.7
SPCC4B3.06c |||NADPH-dependent FMN reductase |Schizosaccharomyce... 25 9.7
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 121 bits (291), Expect = 1e-28
Identities = 58/108 (53%), Positives = 74/108 (68%), Gaps = 1/108 (0%)
Frame = +3
Query: 255 IAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATV 434
+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+ +
Sbjct: 76 VATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNYVII 135
Query: 435 IGHNPDAKPSRVKLPVWSQEGLPSRTEH-GRYCCWRWTYCKPILKAGR 575
+GH+ D +RVKLP +++ +PS KP+LKAGR
Sbjct: 136 VGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGR 183
Score = 104 bits (250), Expect = 1e-23
Identities = 64/169 (37%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +1
Query: 46 AQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDP 225
AQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA V FR+P
Sbjct: 7 AQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAKVAFRNP 65
Query: 226 YKFKTRKELLLLPKGSTQANLFIVERKQXXXXXXXXXXXXXXRVPLCAILKRKWVIEVVW 405
Y ++T E + +G + + + ++ K
Sbjct: 66 YHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGAL 125
Query: 406 HVPLETSPL*LDTILMLSRQE*SYPSGAKKVC-HQEQSMVGIVAGGGRI 549
+ + + + PSGAKKV + +VGIVAGGGRI
Sbjct: 126 GRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRI 174
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 121 bits (291), Expect = 1e-28
Identities = 58/108 (53%), Positives = 74/108 (68%), Gaps = 1/108 (0%)
Frame = +3
Query: 255 IAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATV 434
+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+ +
Sbjct: 76 VATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNYVII 135
Query: 435 IGHNPDAKPSRVKLPVWSQEGLPSRTEH-GRYCCWRWTYCKPILKAGR 575
+GH+ D +RVKLP +++ +PS KP+LKAGR
Sbjct: 136 VGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGR 183
Score = 104 bits (250), Expect = 1e-23
Identities = 64/169 (37%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +1
Query: 46 AQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDP 225
AQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA V FR+P
Sbjct: 7 AQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAKVAFRNP 65
Query: 226 YKFKTRKELLLLPKGSTQANLFIVERKQXXXXXXXXXXXXXXRVPLCAILKRKWVIEVVW 405
Y ++T E + +G + + + ++ K
Sbjct: 66 YHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGAL 125
Query: 406 HVPLETSPL*LDTILMLSRQE*SYPSGAKKVC-HQEQSMVGIVAGGGRI 549
+ + + + PSGAKKV + +VGIVAGGGRI
Sbjct: 126 GRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRI 174
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 121 bits (291), Expect = 1e-28
Identities = 58/108 (53%), Positives = 74/108 (68%), Gaps = 1/108 (0%)
Frame = +3
Query: 255 IAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATV 434
+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+ +
Sbjct: 76 VATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNYVII 135
Query: 435 IGHNPDAKPSRVKLPVWSQEGLPSRTEH-GRYCCWRWTYCKPILKAGR 575
+GH+ D +RVKLP +++ +PS KP+LKAGR
Sbjct: 136 VGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRIDKPLLKAGR 183
Score = 104 bits (250), Expect = 1e-23
Identities = 64/169 (37%), Positives = 89/169 (52%), Gaps = 1/169 (0%)
Frame = +1
Query: 46 AQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDP 225
AQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA V FR+P
Sbjct: 7 AQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAKVAFRNP 65
Query: 226 YKFKTRKELLLLPKGSTQANLFIVERKQXXXXXXXXXXXXXXRVPLCAILKRKWVIEVVW 405
Y ++T E + +G + + + ++ K
Sbjct: 66 YHYRTDVETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGAL 125
Query: 406 HVPLETSPL*LDTILMLSRQE*SYPSGAKKVC-HQEQSMVGIVAGGGRI 549
+ + + + PSGAKKV + +VGIVAGGGRI
Sbjct: 126 GRSSGNYVIIVGHDVDTGKTRVKLPSGAKKVVPSSARGVVGIVAGGGRI 174
>SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 27.9 bits (59), Expect = 1.4
Identities = 17/55 (30%), Positives = 27/55 (49%), Gaps = 2/55 (3%)
Frame = -2
Query: 353 LRHSSNRHHISNFKSCFLSTINKLACVEPFGSNKSSFLVLN--LYGSRKCTTAKG 195
L SS HH+ +ST L ++ +K+ F+VLN +Y S K + +G
Sbjct: 234 LNTSSESHHLLELLGLRISTFMSLGDIDKELISKTDFVVLNNAVYDSEKISFPEG 288
>SPAC9.08c |||steroid reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 282
Score = 27.9 bits (59), Expect = 1.4
Identities = 13/39 (33%), Positives = 18/39 (46%)
Frame = -3
Query: 622 TPRHMPQYVXLVLVEXLPAFKIGLQYVHLQQQYRPCSVL 506
+P H +V VL L IGL V Q+ P S++
Sbjct: 117 SPMHWTVFVSAVLFNFLNGMSIGLYLVQASVQHHPVSII 155
>SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 568
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/28 (35%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = -3
Query: 535 QQQYRPCSVLDGR-PSWLQTGSFTLDGL 455
++ PC+V+ PSW++ G+F L G+
Sbjct: 197 RETVEPCAVVCRMAPSWIRIGTFDLQGI 224
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -3
Query: 397 PLSPIFSSRLHTMVPSGIAPTGITFPTSRVAFF 299
P++P+F SR H P G+ T FP F+
Sbjct: 11 PINPVFPSRAHPSPPLGL--TSNLFPIQTNKFY 41
>SPAC1952.13 |ned1||lipin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 656
Score = 25.4 bits (53), Expect = 7.3
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = +3
Query: 423 FATVIGHNPDAKPSR 467
FA V GH+P A PSR
Sbjct: 279 FAEVYGHSPPASPSR 293
>SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein
L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 197
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +1
Query: 145 YIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKELLLLPKGSTQANL 288
Y+ + K ++P RGA HFR P + T+ +LP +T+ N+
Sbjct: 55 YLAYLRKACRYNPSRGA----FHFRAPSRIFTKAVRGMLPHKTTRGNI 98
>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 409
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = -1
Query: 318 LQELLSFHNKQIGLCRALREQ*ELLPCLELVWIAEVYN 205
LQ+++ F + G+C L + ELL + + + V+N
Sbjct: 305 LQDMVLFRHGSTGVCNVLAKAMELLNLIPVFPVKNVHN 342
>SPCC645.13 |||transcription elongation
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +1
Query: 49 QRKGAGSVFVSHTKKRKGAPKLRSLD 126
+R+G+ +HTK+ K APK D
Sbjct: 167 KRRGSVGTTATHTKRSKNAPKTSPKD 192
>SPCC4B3.06c |||NADPH-dependent FMN reductase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 200
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/54 (27%), Positives = 24/54 (44%), Gaps = 4/54 (7%)
Frame = +3
Query: 312 LEVGNVMPVGAMPEGTIVCNLEEKMGD----RGRLARASGNFATVIGHNPDAKP 461
+ V MP +P IV + + + G L +A G F+ + NP+ KP
Sbjct: 147 MRVAPTMPALTLPRDKIVQGVVDPAVEFTKHLGELKKAFGEFSQLFESNPERKP 200
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,782,459
Number of Sequences: 5004
Number of extensions: 59198
Number of successful extensions: 165
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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