Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0230
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A) 124 5e-29
At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) riboso... 123 1e-28
At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) riboso... 122 3e-28
At2g44065.2 68415.m05480 ribosomal protein L2 family protein sim... 38 0.006
At2g44065.1 68415.m05479 ribosomal protein L2 family protein sim... 38 0.006
At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate 5-ki... 31 0.90
At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17 si... 29 2.1
At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17 si... 29 2.1
At3g45860.1 68416.m04963 receptor-like protein kinase, putative ... 29 3.6
At2g42730.1 68415.m05292 F-box family protein contains F-box dom... 29 3.6
At5g20860.1 68418.m02477 pectinesterase family protein contains ... 28 6.3
At1g70380.1 68414.m08096 F-box family protein contains Pfam PF00... 28 6.3
At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1, plast... 27 8.4
At1g21980.1 68414.m02750 1-phosphatidylinositol-4-phosphate 5-ki... 27 8.4
>At2g18020.1 68415.m02094 60S ribosomal protein L8 (RPL8A)
Length = 258
Score = 124 bits (299), Expect = 5e-29
Identities = 62/123 (50%), Positives = 86/123 (69%), Gaps = 2/123 (1%)
Frame = +3
Query: 210 TLPRSIQVQDKEGALIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMG 389
T + + ++ +A EG+YTGQF+YCGKKATL VGNV+P+ ++PEG +VCN+E +G
Sbjct: 62 TFRHPFRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVVCNVEHHVG 121
Query: 390 DRGRLARASGNFATVIGHNPDAKPSRVKLPVWSQEGLPS--RTEHGRYCCWRWTYCKPIL 563
DRG LARASG++A VI HNPD+ +R+KLP S++ +PS R G+ T KP+L
Sbjct: 122 DRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGRTE-KPML 180
Query: 564 KAG 572
KAG
Sbjct: 181 KAG 183
Score = 111 bits (268), Expect = 3e-25
Identities = 74/176 (42%), Positives = 97/176 (55%), Gaps = 9/176 (5%)
Frame = +1
Query: 46 AQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDP 225
AQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P
Sbjct: 7 AQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVTFRHP 66
Query: 226 YKFKTRKELLLLPKGSTQANLFIVERKQXXXXXXXXXXXXXXRVPLCAILKRKWVIEVVW 405
++FK +KEL + +G +K +PL +I + V V
Sbjct: 67 FRFKKQKELFVAAEGMYTGQFLYCGKKATLVVGNV--------LPLRSIPEGAVVCNVEH 118
Query: 406 HVPLE--TSPL*LDTILMLSRQE*S------YPSGAKKVCHQE-QSMVGIVAGGGR 546
HV + D ++++ S PSG+KK+ ++M+G VAGGGR
Sbjct: 119 HVGDRGVLARASGDYAIVIAHNPDSDTTRIKLPSGSKKIVPSGCRAMIGQVAGGGR 174
>At3g51190.1 68416.m05604 60S ribosomal protein L8 (RPL8B) ribosomal
protein L8, cytosolic - Arabidopsis thaliana, PIR:T04582
Length = 260
Score = 123 bits (296), Expect = 1e-28
Identities = 60/108 (55%), Positives = 80/108 (74%), Gaps = 2/108 (1%)
Frame = +3
Query: 255 IAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATV 434
+A EG+YTGQ++YCGKKA L VGNV+P+G++PEG ++CN+E +GDRG LARASG++A V
Sbjct: 78 VAAEGMYTGQYLYCGKKANLMVGNVLPLGSIPEGAVICNVELHVGDRGALARASGDYAIV 137
Query: 435 IGHNPDAKPSRVKLPVWSQEGLPS--RTEHGRYCCWRWTYCKPILKAG 572
I HNP++ +RVKLP S++ LPS R G+ T KP LKAG
Sbjct: 138 IAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTE-KPFLKAG 184
Score = 105 bits (251), Expect = 3e-23
Identities = 73/184 (39%), Positives = 98/184 (53%), Gaps = 10/184 (5%)
Frame = +1
Query: 46 AQRKGA-GSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRD 222
AQRKGA GSVF SHT RKG K RSLDY ER+GY+KG+V +IIHDPGRGAPLA V FR
Sbjct: 7 AQRKGAAGSVFKSHTHHRKGPAKFRSLDYGERNGYLKGLVTEIIHDPGRGAPLARVAFRH 66
Query: 223 PYKFKTRKELLLLPKGSTQANLFIVERKQXXXXXXXXXXXXXXRVPLCAILKRKWVIEVV 402
P+++ +KEL + +G +K +PL +I + + V
Sbjct: 67 PFRYMKQKELFVAAEGMYTGQYLYCGKKANLMVGNV--------LPLGSIPEGAVICNVE 118
Query: 403 WHVPLETSPL*L--DTILMLSRQE*S------YPSGAKKVCHQE-QSMVGIVAGGGRIAN 555
HV + D ++++ S PSG+KK+ ++M+G VAGGGR
Sbjct: 119 LHVGDRGALARASGDYAIVIAHNPESNTTRVKLPSGSKKILPSACRAMIGQVAGGGRTEK 178
Query: 556 LF*K 567
F K
Sbjct: 179 PFLK 182
>At4g36130.1 68417.m05142 60S ribosomal protein L8 (RPL8C) ribosomal
protein L8, cytosolic, tomato, PIR1:R5TOL8
Length = 258
Score = 122 bits (293), Expect = 3e-28
Identities = 60/108 (55%), Positives = 79/108 (73%), Gaps = 2/108 (1%)
Frame = +3
Query: 255 IAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATV 434
+A EG+YTGQF+YCGKKATL VGNV+P+ ++PEG ++CN+E +GDRG ARASG++A V
Sbjct: 77 VAAEGMYTGQFLYCGKKATLVVGNVLPLRSIPEGAVICNVEHHVGDRGVFARASGDYAIV 136
Query: 435 IGHNPDAKPSRVKLPVWSQEGLPS--RTEHGRYCCWRWTYCKPILKAG 572
I HNPD SR+KLP S++ +PS R G+ T KP+LKAG
Sbjct: 137 IAHNPDNDTSRIKLPSGSKKIVPSGCRAMIGQVAGGGRTE-KPMLKAG 183
Score = 111 bits (266), Expect = 5e-25
Identities = 51/75 (68%), Positives = 60/75 (80%)
Frame = +1
Query: 46 AQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAVVHFRDP 225
AQRKGAGSVF SHT RKG K RSLD+ ER+GY+KGVV +IIHDPGRGAPLA V FR P
Sbjct: 7 AQRKGAGSVFKSHTHHRKGPAKFRSLDFGERNGYLKGVVTEIIHDPGRGAPLARVAFRHP 66
Query: 226 YKFKTRKELLLLPKG 270
++FK +KEL + +G
Sbjct: 67 FRFKKQKELFVAAEG 81
>At2g44065.2 68415.m05480 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 37.9 bits (84), Expect = 0.006
Identities = 19/54 (35%), Positives = 30/54 (55%)
Frame = +3
Query: 318 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKPSRVKLP 479
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P +KLP
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLP 111
>At2g44065.1 68415.m05479 ribosomal protein L2 family protein
similar to ribosomal protein L2 [Gossypium arboreum]
GI:17644114; contains Pfam profile PF03947: Ribosomal
Proteins L2, C-terminal domain
Length = 214
Score = 37.9 bits (84), Expect = 0.006
Identities = 19/54 (35%), Positives = 30/54 (55%)
Frame = +3
Query: 318 VGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKPSRVKLP 479
+G+ MP+G M GTI+ N+E G ++ RA+G A ++ P +KLP
Sbjct: 59 IGSSMPLGMMRIGTIIHNIEMNPGQGAKMVRAAGTNAKIL-KEPAKGKCLIKLP 111
>At2g26420.1 68415.m03170 1-phosphatidylinositol-4-phosphate
5-kinase, putative / PIP kinase, putative /
PtdIns(4)P-5-kinase, putative / diphosphoinositide
kinase, putative similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 705
Score = 30.7 bits (66), Expect = 0.90
Identities = 13/38 (34%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = -2
Query: 329 HISNFKSCFLSTINKLACVEPFGSNKSSFLVL-NLYGS 219
H+S +K+ ++ + CV+P G K+ F+V+ NL+ S
Sbjct: 462 HVSKYKNSLVTKFFGVHCVKPVGGQKTRFIVMGNLFCS 499
>At2g43660.2 68415.m05427 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 123
Score = 29.5 bits (63), Expect = 2.1
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -2
Query: 281 ACVEPFGSNKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 153
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 70 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 112
>At2g43660.1 68415.m05426 glycosyl hydrolase family protein 17
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]; C terminal homology
only
Length = 122
Score = 29.5 bits (63), Expect = 2.1
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -2
Query: 281 ACVEPFGSNKSSFLVLNLYGSRKCTTAKGAPLPGSWIISLTTP 153
AC +P S+ +V+NLY + GS IIS+T P
Sbjct: 69 ACYDPINLYNSASVVMNLYYQNQGRQYSKCDFEGSGIISVTDP 111
>At3g45860.1 68416.m04963 receptor-like protein kinase, putative
similar to receptor-like protein kinase 4 (gi:13506745),
5 (gi:13506747), and 6 (gi:13506749) from Arabidopsis
thaliana; contains Pfam protein kinase domain PF00069
Length = 676
Score = 28.7 bits (61), Expect = 3.6
Identities = 17/52 (32%), Positives = 28/52 (53%)
Frame = -2
Query: 398 TSITHFLFKIAHNGTLRHSSNRHHISNFKSCFLSTINKLACVEPFGSNKSSF 243
+++T+ I NG SSN++ + F+ ST+N LA VE S+K +
Sbjct: 137 STVTYDGSAILLNGANISSSNQNQVDEFRDLVSSTLN-LAAVEAANSSKKFY 187
>At2g42730.1 68415.m05292 F-box family protein contains F-box domain
Pfam:PF00646
Length = 737
Score = 28.7 bits (61), Expect = 3.6
Identities = 19/53 (35%), Positives = 27/53 (50%)
Frame = -1
Query: 333 ASHFQLQELLSFHNKQIGLCRALREQ*ELLPCLELVWIAEVYNSQRCTSTRVM 175
+S ++ E+LS+ L ++ E LPCLELV I V NS +T M
Sbjct: 382 SSQVKVLEILSYQGTTREL-NQMKHFLEKLPCLELVKICVVNNSNNLQTTMEM 433
>At5g20860.1 68418.m02477 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 512
Score = 27.9 bits (59), Expect = 6.3
Identities = 14/58 (24%), Positives = 22/58 (37%)
Frame = +3
Query: 324 NVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNFATVIGHNPDAKPSRVKLPVWSQEG 497
+++ G + + ++ +KM RL S I NP K LP W G
Sbjct: 160 SILDSGGSSSASAISHISQKMDHLSRLVSNSLTLVDTIMKNPKPKTKSTALPRWVTAG 217
>At1g70380.1 68414.m08096 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640: F-box
protein interaction domain
Length = 377
Score = 27.9 bits (59), Expect = 6.3
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +3
Query: 249 ALIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKM 386
+++ EGL+ G F+Y G +++ VM E V L E++
Sbjct: 207 SVVTDEGLFNGGFLYYGARSSCNKPMVMRFNVSSEAFAVIKLPEEV 252
>At3g16950.1 68416.m02166 dihydrolipoamide dehydrogenase 1,
plastidic / lipoamide dehydrogenase 1 (PTLPD1) identical
to plastidic lipoamide dehydrogenase from Arabidopsis
thaliana [gi:7159282]
Length = 570
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -2
Query: 428 GEVSRGTCQTTSITHFLFKIAHNGTLRHSSNRHHISNF 315
G+V GTC +A +G +R N HH+ +F
Sbjct: 117 GDVVGGTCVNRGCVPSKALLAVSGRMRELQNEHHMKSF 154
>At1g21980.1 68414.m02750 1-phosphatidylinositol-4-phosphate
5-kinase, putative / PIP kinase, putative /
PtdIns(4)P-5-kinase, putative / diphosphoinositide
kinase, putative strong similarity to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 752
Score = 27.5 bits (58), Expect = 8.4
Identities = 11/38 (28%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = -2
Query: 329 HISNFKSCFLSTINKLACVEPFGSNKSSFLVL-NLYGS 219
H+ +++ ++ + CV+P G K+ F+V+ NL+ S
Sbjct: 488 HVCQYENSLVTRFYGVHCVKPVGGQKTRFIVMGNLFCS 525
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,979,008
Number of Sequences: 28952
Number of extensions: 327464
Number of successful extensions: 862
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 859
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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