Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0229
(666 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 92 8e-20
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 84 2e-17
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 42 6e-05
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 40 4e-04
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 36 0.004
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 34 0.016
SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces pombe... 27 1.8
SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit ... 26 4.2
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 26 4.2
SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 26 5.6
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 25 9.8
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 91.9 bits (218), Expect = 8e-20
Identities = 43/93 (46%), Positives = 60/93 (64%), Gaps = 1/93 (1%)
Frame = +3
Query: 264 YFLDDLDRLGAKDYQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIH 443
Y+ D +DR+ Y P++QDIL +KTTGI E F +++ FDVGGQ SER+KWIH
Sbjct: 194 YYQDHIDRIFDPQYIPSDQDILHCRIKTTGISEETFLLNRHHYRFFDVGGQRSERRKWIH 253
Query: 444 CFEDVTAIIFXVPCLNMXRXCTG*NT-NRMQES 539
CFE+VTA++F V + N+ N+MQE+
Sbjct: 254 CFENVTALLFLVSLAGYDQCLVEDNSGNQMQEA 286
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 84.2 bits (199), Expect = 2e-17
Identities = 37/75 (49%), Positives = 51/75 (68%)
Frame = +3
Query: 255 SAKYFLDDLDRLGAKDYQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFDVGGQXSERKK 434
SA YF D + ++ Y PT DILR+ T GI E+ F+ +L ++FDVGGQ +ER+K
Sbjct: 149 SAPYFFSRADEICSRHYVPTIDDILRSRNSTLGISEISFTLDHLQIRMFDVGGQRTERRK 208
Query: 435 WIHCFEDVTAIIFXV 479
WI+CFE+V +IIF V
Sbjct: 209 WIYCFENVNSIIFCV 223
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 42.3 bits (95), Expect = 6e-05
Identities = 26/85 (30%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +3
Query: 231 FQRVPTQRSAKYFLDDLDRLGAKD--YQPTEQDILRTXVKTTGIXEVHFSFKNLNFKLFD 404
FQ + +R + + LD G Y+ +I+ T + T G ++N++F ++D
Sbjct: 9 FQSLFGKREMRILMVGLDAAGKTTILYKLKLGEIVTT-IPTIGFNVETVEYRNISFTVWD 67
Query: 405 VGGQXSERKKWIHCFEDVTAIIFXV 479
VGGQ R W H F++ IIF V
Sbjct: 68 VGGQDKIRPLWRHYFQNTQGIIFVV 92
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 39.5 bits (88), Expect = 4e-04
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +3
Query: 339 VKTTGIXEVHFSFKNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
+ T G ++KN+ F ++DVGGQ R W H F +IF V + R
Sbjct: 50 IPTVGFNVETVTYKNIKFNVWDVGGQDKIRPLWRHYFTGTKGLIFVVDSADSNR 103
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 36.3 bits (80), Expect = 0.004
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +3
Query: 381 NLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
N+ F FD+GG R+ W F +V I++ V C + R
Sbjct: 63 NVRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFER 102
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 34.3 bits (75), Expect = 0.016
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = +3
Query: 378 KNLNFKLFDVGGQXSERKKWIHCFEDVTAIIFXVPCLNMXR 500
+ L F ++D+GGQ + R W + FE AII+ V L+ R
Sbjct: 58 EGLRFTIWDIGGQKTLRNFWKNYFESTEAIIWVVDSLDDLR 98
>SPBC20F10.05 |||DuF1740 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 972
Score = 27.5 bits (58), Expect = 1.8
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +3
Query: 372 SFKNLNFKLFD 404
SFKNLNFKLFD
Sbjct: 553 SFKNLNFKLFD 563
>SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit
Rrn6 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 868
Score = 26.2 bits (55), Expect = 4.2
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +1
Query: 202 DSGVQECFGRSNEYQLNDPLNISWTTSTG*EQRITSPPNKI 324
D G +ECFG S + L L + T+S + +P N I
Sbjct: 306 DDGYRECFGSSFKESLQQALCSAPTSSIAYHEEEINPDNTI 346
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 26.2 bits (55), Expect = 4.2
Identities = 17/56 (30%), Positives = 33/56 (58%), Gaps = 5/56 (8%)
Frame = -1
Query: 246 LVLVGTTKALLNARVGPEPFHRGEKLLTERFRVLHPLN-----HVEHHLTIAFSVS 94
L+++ T ++L A +GP +KL ERF++L +N +++ ++ AFS+S
Sbjct: 1469 LMIISTVDSVL-ATIGPTITGWMKKLDHERFKILAGINLCNLIYLKRYIKYAFSIS 1523
>SPBC27B12.12c |||CorA family magnesium ion transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 25.8 bits (54), Expect = 5.6
Identities = 10/25 (40%), Positives = 15/25 (60%)
Frame = -3
Query: 217 PERQSRPRAFSSRREAPH*TVPCPP 143
P R S ++ S+ + PH T+P PP
Sbjct: 275 PRRPSATKSCSAAVDCPHTTIPKPP 299
>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 991
Score = 25.0 bits (52), Expect = 9.8
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -3
Query: 97 LYCHR*CPSWAWH 59
LY ++ CPSW W+
Sbjct: 626 LYYYQGCPSWTWY 638
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,653,077
Number of Sequences: 5004
Number of extensions: 52332
Number of successful extensions: 143
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 143
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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