Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0183
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 178 5e-46
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 98 9e-22
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 94 1e-20
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 88 1e-18
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 62 7e-11
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 58 1e-09
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 49 7e-07
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 32 0.064
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 31 0.11
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.59
SPCC1442.07c |||ubiquitin/metalloprotease fusion protein|Schizos... 27 1.8
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 3.2
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.2
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 25 9.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 178 bits (434), Expect = 5e-46
Identities = 79/84 (94%), Positives = 81/84 (96%)
Frame = +3
Query: 255 GQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 434
GQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE
Sbjct: 48 GQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTE 107
Query: 435 APLNPKANREKMTQIMFETFNTPA 506
APLNPK+NREKMTQI+FETFN PA
Sbjct: 108 APLNPKSNREKMTQIIFETFNAPA 131
Score = 96.7 bits (230), Expect = 3e-21
Identities = 41/49 (83%), Positives = 47/49 (95%)
Frame = +1
Query: 118 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMDRR 264
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGM ++
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQK 50
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 98.3 bits (234), Expect = 9e-22
Identities = 43/84 (51%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
Frame = +3
Query: 258 QKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTE 434
QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEHP+LLTE
Sbjct: 53 QKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTE 112
Query: 435 APLNPKANREKMTQIMFETFNTPA 506
PL N+EK+ + +ET N PA
Sbjct: 113 PPLANIRNKEKIAEYFYETLNVPA 136
Score = 55.2 bits (127), Expect = 8e-09
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVM 246
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVM 48
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 94.3 bits (224), Expect = 1e-20
Identities = 38/85 (44%), Positives = 61/85 (71%)
Frame = +3
Query: 240 RDGRYGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 419
+ G KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P
Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102
Query: 420 VLLTEAPLNPKANREKMTQIMFETF 494
+LLTE P+NP ANREKM + MFE +
Sbjct: 103 ILLTEPPMNPVANREKMCETMFERY 127
Score = 48.0 bits (109), Expect = 1e-06
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +1
Query: 124 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 228
E A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 87.8 bits (208), Expect = 1e-18
Identities = 38/79 (48%), Positives = 50/79 (63%), Gaps = 1/79 (1%)
Frame = +3
Query: 264 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 440
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 441 LNPKANREKMTQIMFETFN 497
LNP NRE +IMFE+FN
Sbjct: 133 LNPPENRENTAEIMFESFN 151
Score = 40.7 bits (91), Expect = 2e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 219
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 62.1 bits (144), Expect = 7e-11
Identities = 29/90 (32%), Positives = 53/90 (58%), Gaps = 1/90 (1%)
Frame = +3
Query: 240 RDGRYGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 416
RD + + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EH
Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116
Query: 417 PVLLTEAPLNPKANREKMTQIMFETFNTPA 506
P+ +TE NP R MT+++FE +N P+
Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPS 146
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 231
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 58.0 bits (134), Expect = 1e-09
Identities = 26/67 (38%), Positives = 37/67 (55%)
Frame = +3
Query: 306 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 485
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 486 ETFNTPA 506
E+ PA
Sbjct: 123 ESLRCPA 129
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +1
Query: 121 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 222
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 48.8 bits (111), Expect = 7e-07
Identities = 24/84 (28%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
Frame = +3
Query: 261 KDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 434
++ YV DE Q I + +K +GI+ +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 435 APLNPKANREKMTQIMFETFNTPA 506
NP++ R+++ + FE + PA
Sbjct: 109 PSWNPQSVRQQIMEAAFEQLHVPA 132
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +1
Query: 121 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 222
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 32.3 bits (70), Expect = 0.064
Identities = 20/65 (30%), Positives = 29/65 (44%), Gaps = 4/65 (6%)
Frame = +3
Query: 321 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 488
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 489 TFNTP 503
P
Sbjct: 172 ECQVP 176
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.5 bits (68), Expect = 0.11
Identities = 15/43 (34%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +3
Query: 381 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTP 503
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 29.1 bits (62), Expect = 0.59
Identities = 19/66 (28%), Positives = 29/66 (43%)
Frame = -3
Query: 353 IPVSDDSVFDGVFEGQDTSFALCLISYIRVLLSIPTITP*WRGLPTIEGNTARGASSPAK 174
+P + +V +TSFAL S L PT + P +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 173 PALHIP 156
P L+ P
Sbjct: 123 PQLNSP 128
>SPCC1442.07c |||ubiquitin/metalloprotease fusion
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 282
Score = 27.5 bits (58), Expect = 1.8
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -2
Query: 291 SVPHLLHKSPSVHTDHHALMAGPSHDRGEH 202
+VP L P+ HT H + G +H++G H
Sbjct: 148 TVPLLSEMDPAEHTRHDSKTLGLNHNQGAH 177
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.6 bits (56), Expect = 3.2
Identities = 15/45 (33%), Positives = 18/45 (40%)
Frame = -3
Query: 251 PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 117
PT+ P P N S P PL+TTN TS+S
Sbjct: 411 PTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 423 LLTEAPLNPKANREKMTQIMFETFN 497
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 25.0 bits (52), Expect = 9.7
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = -1
Query: 544 RTRQREHGXMPTHAGVLNVSNMIWVIFSLLALGLRGASVSRTGCSSG 404
RT++ E+G T L + +IF L LG +S++ C SG
Sbjct: 470 RTQELENGMENTKDSTLMKPEALGIIFGPLLLGNSSTDLSKS-CPSG 515
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,625,491
Number of Sequences: 5004
Number of extensions: 54774
Number of successful extensions: 142
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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