Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0127
(661 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|... 134 1e-32
SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein ... 134 1e-32
SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein L... 134 1e-32
SPAC3F10.10c |map3||pheromone M-factor receptor |Schizosaccharom... 31 0.19
SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein L13/L16|Schizos... 27 1.8
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 26 5.5
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 5.5
SPCC645.13 |||transcription elongation regulator|Schizosaccharom... 25 9.7
>SPAC1F7.13c |rpl801|rpl8-1, rpl18, rpk5a, rpl2-1, SPAC21E11.02c|60S
ribosomal protein L8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 253
Score = 134 bits (323), Expect = 1e-32
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = +2
Query: 254 ELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNF 433
E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+
Sbjct: 73 ETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNY 132
Query: 434 ATVIGHNPDAKRTRVKLPSGAKKVL 508
++GH+ D +TRVKLPSGAKKV+
Sbjct: 133 VIIVGHDVDTGKTRVKLPSGAKKVV 157
Score = 110 bits (264), Expect = 2e-25
Identities = 51/81 (62%), Positives = 66/81 (81%)
Frame = +3
Query: 36 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 215
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 216 VHFRDPYKFKTRKSSSLLPKG 278
V FR+PY ++T + + +G
Sbjct: 60 VAFRNPYHYRTDVETFVATEG 80
Score = 93.5 bits (222), Expect = 2e-20
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = +1
Query: 511 SSNRGMVGIVAGGGRIDKPILKAGRAYHKXKVKRNCWPYVXGVAMNPVEH 660
SS RG+VGIVAGGGRIDKP+LKAGRA+HK +VKRNCWP GVAMNPV+H
Sbjct: 159 SSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTRGVAMNPVDH 208
>SPBC2F12.07c |rpl802|rpl8-2, rpk37, rpk5b|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 134 bits (323), Expect = 1e-32
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = +2
Query: 254 ELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNF 433
E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+
Sbjct: 73 ETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNY 132
Query: 434 ATVIGHNPDAKRTRVKLPSGAKKVL 508
++GH+ D +TRVKLPSGAKKV+
Sbjct: 133 VIIVGHDVDTGKTRVKLPSGAKKVV 157
Score = 110 bits (264), Expect = 2e-25
Identities = 51/81 (62%), Positives = 66/81 (81%)
Frame = +3
Query: 36 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 215
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 216 VHFRDPYKFKTRKSSSLLPKG 278
V FR+PY ++T + + +G
Sbjct: 60 VAFRNPYHYRTDVETFVATEG 80
Score = 93.5 bits (222), Expect = 2e-20
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = +1
Query: 511 SSNRGMVGIVAGGGRIDKPILKAGRAYHKXKVKRNCWPYVXGVAMNPVEH 660
SS RG+VGIVAGGGRIDKP+LKAGRA+HK +VKRNCWP GVAMNPV+H
Sbjct: 159 SSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTRGVAMNPVDH 208
>SPBC839.04 |rpl803|rpl8-3, rpk5-b, rpkD4|60S ribosomal protein
L8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 253
Score = 134 bits (323), Expect = 1e-32
Identities = 58/85 (68%), Positives = 70/85 (82%)
Frame = +2
Query: 254 ELFIAPEGLYTGQFVYCGKKATLEVGNVMPVGAMPEGTIVCNLEEKMGDRGRLARASGNF 433
E F+A EG+YTGQFVYCGK A L VGNV+PVG MPEGTI+ N+EEK GDRG L R+SGN+
Sbjct: 73 ETFVATEGMYTGQFVYCGKNAALTVGNVLPVGEMPEGTIISNVEEKAGDRGALGRSSGNY 132
Query: 434 ATVIGHNPDAKRTRVKLPSGAKKVL 508
++GH+ D +TRVKLPSGAKKV+
Sbjct: 133 VIIVGHDVDTGKTRVKLPSGAKKVV 157
Score = 110 bits (264), Expect = 2e-25
Identities = 51/81 (62%), Positives = 66/81 (81%)
Frame = +3
Query: 36 MGRVIRAQRKGAGSVFVSHTKKRKGAPKLRSLDYAERHGYIKGVVKDIIHDPGRGAPLAV 215
MGRVIRAQRK +G +F +HT+ RKGA +LR+LD+AERHGYI+GVV+ IIHDPGRGAPLA
Sbjct: 1 MGRVIRAQRK-SGGIFQAHTRLRKGAAQLRTLDFAERHGYIRGVVQKIIHDPGRGAPLAK 59
Query: 216 VHFRDPYKFKTRKSSSLLPKG 278
V FR+PY ++T + + +G
Sbjct: 60 VAFRNPYHYRTDVETFVATEG 80
Score = 93.5 bits (222), Expect = 2e-20
Identities = 40/50 (80%), Positives = 45/50 (90%)
Frame = +1
Query: 511 SSNRGMVGIVAGGGRIDKPILKAGRAYHKXKVKRNCWPYVXGVAMNPVEH 660
SS RG+VGIVAGGGRIDKP+LKAGRA+HK +VKRNCWP GVAMNPV+H
Sbjct: 159 SSARGVVGIVAGGGRIDKPLLKAGRAFHKYRVKRNCWPRTRGVAMNPVDH 208
>SPAC3F10.10c |map3||pheromone M-factor receptor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 365
Score = 30.7 bits (66), Expect = 0.19
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -3
Query: 581 PAFKIGLSIRPPPATIPTMPLLLDAEPSWLQTVALLLYA 465
P K+GL+ PP + M L W + VAL+L++
Sbjct: 255 PTTKVGLNDWVPPTVLYLMSLFFSTSGGWTEKVALILWS 293
>SPBC2G2.05 |rpl1603|rpl16c|60S ribosomal protein
L13/L16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 197
Score = 27.5 bits (58), Expect = 1.8
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +3
Query: 153 YIKGVVKDIIHDPGRGAPLAVVHFRDPYKFKTRKSSSLLPKGSTQANL 296
Y+ + K ++P RGA HFR P + T+ +LP +T+ N+
Sbjct: 55 YLAYLRKACRYNPSRGA----FHFRAPSRIFTKAVRGMLPHKTTRGNI 98
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.8 bits (54), Expect = 5.5
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 252 KSSSLLPKGSTQANLFIVE 308
KS+++LP GST LF++E
Sbjct: 1353 KSNAILPHGSTSDKLFLLE 1371
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = -2
Query: 405 PLSPIFSSRLHTMVPSGIAPTGITFPTSRVAFF 307
P++P+F SR H P G+ T FP F+
Sbjct: 11 PINPVFPSRAHPSPPLGL--TSNLFPIQTNKFY 41
>SPCC645.13 |||transcription elongation
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 25.0 bits (52), Expect = 9.7
Identities = 10/26 (38%), Positives = 15/26 (57%)
Frame = +3
Query: 57 QRKGAGSVFVSHTKKRKGAPKLRSLD 134
+R+G+ +HTK+ K APK D
Sbjct: 167 KRRGSVGTTATHTKRSKNAPKTSPKD 192
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,841,914
Number of Sequences: 5004
Number of extensions: 60535
Number of successful extensions: 184
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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