BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0124 (680 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 28 1.1 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 27 2.5 SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces ... 27 3.3 SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 26 4.4 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 4.4 SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 26 4.4 SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 26 4.4 SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schi... 26 4.4 SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccha... 26 5.8 SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c... 26 5.8 SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch... 26 5.8 SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 25 7.7 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 28.3 bits (60), Expect = 1.1 Identities = 22/75 (29%), Positives = 35/75 (46%) Frame = -3 Query: 531 SKKAAASDLPDCISASVSSCIETPKLFPSSIFGIINEIHILSKFESSTSSLMCGLTRCIR 352 S ++S +P S SVSS + + PSS I+ S F S+++S ++ I Sbjct: 561 SSGISSSSIPSTFS-SVSSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSIS 619 Query: 351 SLSGLLQGTEPFGST 307 S S +L P S+ Sbjct: 620 SSSTILSSPTPSTSS 634 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 27.1 bits (57), Expect = 2.5 Identities = 13/51 (25%), Positives = 29/51 (56%), Gaps = 1/51 (1%) Frame = +3 Query: 75 LKAEHLIIKGFPEKIVKLNELLETS-NFQNRNLSDVHQDLNIPIPTPPATS 224 +K E + + P++ + E ++TS + N+S + + ++P+PT AT+ Sbjct: 524 IKEEEAVSENAPQQEERSFEQIKTSIHIAPENISAIEESKSVPLPTSFATT 574 >SPCC736.03c |||phenylalanyl-tRNA synthetase|Schizosaccharomyces pombe|chr 3|||Manual Length = 429 Score = 26.6 bits (56), Expect = 3.3 Identities = 13/50 (26%), Positives = 26/50 (52%) Frame = +3 Query: 138 LETSNFQNRNLSDVHQDLNIPIPTPPATSNNEPNAKRQRWTHRSYPATQP 287 LE + F +++L D + + + + ++ R RWT+ S+P T+P Sbjct: 208 LEETKFVSQHLKDTLTTVIHDLVSLAPSISSGAEQVRYRWTYDSFPFTKP 257 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 26.2 bits (55), Expect = 4.4 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +1 Query: 262 IGVIQQLNHRRNTGLCTAEWFSTLQQATQ*SY 357 I V Q L H N G C+ + ST++ + SY Sbjct: 416 IPVFQNLEHNINIGRCSLKKLSTIRSCSTNSY 447 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 26.2 bits (55), Expect = 4.4 Identities = 15/32 (46%), Positives = 18/32 (56%), Gaps = 4/32 (12%) Frame = +2 Query: 335 NKPLSDL----IHLVKPHIRELVEDSNLLKMW 418 N+ L DL IHLVKP++ E N LK W Sbjct: 1072 NQELLDLLLFSIHLVKPNVETNDEVCNTLKAW 1103 >SPBC25H2.03 |||vacuolar protein involved in phosphoinositide metabolism|Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 26.2 bits (55), Expect = 4.4 Identities = 14/31 (45%), Positives = 17/31 (54%), Gaps = 2/31 (6%) Frame = +1 Query: 259 LIGVIQQL--NHRRNTGLCTAEWFSTLQQAT 345 LI V+Q+L N T LC EW LQ+ T Sbjct: 437 LIEVLQKLLSNDNEETRLCALEWVLLLQRRT 467 >SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual Length = 1279 Score = 26.2 bits (55), Expect = 4.4 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = -1 Query: 533 DQKKQLPLIYLIVSLPVYPLVL 468 D K Q+P YL S PV P+VL Sbjct: 358 DLKVQIPFAYLDPSAPVNPIVL 379 >SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 970 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +2 Query: 308 VLPNGSVPCNKPLSDLIHLVKPH 376 VLPNG+ ++D+ H VKP+ Sbjct: 523 VLPNGNYEVGVHIADVTHFVKPN 545 >SPAC20G8.08c |fft1||fun thirty related protein Fft1|Schizosaccharomyces pombe|chr 1|||Manual Length = 944 Score = 25.8 bits (54), Expect = 5.8 Identities = 21/91 (23%), Positives = 40/91 (43%), Gaps = 4/91 (4%) Frame = +2 Query: 419 ISFMIPKIEDGNNFGVSIQEDTLAEIQSGRSEAAAFFDQISRYFISRAKIVSKVAKYPHI 598 ++FM+PK+ D N G+ I A ++ISR + + K + Sbjct: 586 LAFMLPKVFDNNMQGLDIIYKIKTTSDGDIERAYLSQERISRAKTIMNPFILRRRKENVL 645 Query: 599 DDYRRAVRELD----EKEYLSLWLVMCEIRN 679 D ++ ++ E+ LSL+L + E++N Sbjct: 646 SDLPPKIQHVEYCHMEETQLSLYLSVLELKN 676 >SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +3 Query: 156 QNRNLSDVHQDLNIPIPTPPATSNNEPN 239 Q+ ++S H L+IP+P P SNN N Sbjct: 61 QDSSISFTHDPLHIPLPNP---SNNNDN 85 >SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Schizosaccharomyces pombe|chr 2|||Manual Length = 775 Score = 25.8 bits (54), Expect = 5.8 Identities = 8/30 (26%), Positives = 20/30 (66%) Frame = -3 Query: 285 VELLDNSDESIFDV*HLAHCLTSQVVLVLE 196 +E+ ++SDE ++D ++ CL ++L ++ Sbjct: 91 LEIREDSDEDLYDYFYVPKCLVCNIILDIQ 120 >SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 952 Score = 25.4 bits (53), Expect = 7.7 Identities = 18/65 (27%), Positives = 29/65 (44%), Gaps = 2/65 (3%) Frame = +3 Query: 45 KVQDYKDSLKLKAEHLI--IKGFPEKIVKLNELLETSNFQNRNLSDVHQDLNIPIPTPPA 218 ++Q LKL+ E L IK E+ L E LET +NL +++ + + Sbjct: 319 EIQTQNRDLKLETEKLQDQIKALLERNQSLQEALETVKNDEKNLREMNANYETEMKEARQ 378 Query: 219 TSNNE 233 NN+ Sbjct: 379 KLNNK 383 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,842,996 Number of Sequences: 5004 Number of extensions: 61515 Number of successful extensions: 205 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 196 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 205 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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