BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0124
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g28000.1 68417.m04016 AAA-type ATPase family protein contains... 29 3.8
At5g63200.1 68418.m07935 tetratricopeptide repeat (TPR)-containi... 28 6.6
At5g25910.1 68418.m03077 disease resistance family protein conta... 28 6.6
At3g50750.1 68416.m05554 brassinosteroid signalling positive reg... 28 6.6
At3g49430.1 68416.m05403 pre-mRNA splicing factor, putative stro... 28 6.6
At1g65390.2 68414.m07419 disease resistance protein (TIR class),... 28 6.6
At1g65390.1 68414.m07420 disease resistance protein (TIR class),... 28 6.6
At1g44780.1 68414.m05130 expressed protein ; expression supporte... 28 6.6
>At4g28000.1 68417.m04016 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family
Length = 726
Score = 28.7 bits (61), Expect = 3.8
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +2
Query: 443 EDGNNFGVSIQEDTLAEIQSGRSEAAAFFDQISRYFISRAKIVSKVAKYPHI 598
EDG G I+++ + +I GR E++ FD+ Y + +++ A Y H+
Sbjct: 38 EDGLT-GEKIEQELVRQIVDGR-ESSVTFDEFPYYLSEKTRLLLTSAAYVHL 87
>At5g63200.1 68418.m07935 tetratricopeptide repeat (TPR)-containing
protein low similarity to SP|P28290 Sperm-specific
antigen 2 (Cleavage signal-1 protein) (CS-1) Homo
sapiens; contains Pfam profile PF00515: TPR Domain
Length = 649
Score = 27.9 bits (59), Expect = 6.6
Identities = 18/54 (33%), Positives = 29/54 (53%)
Frame = +3
Query: 54 DYKDSLKLKAEHLIIKGFPEKIVKLNELLETSNFQNRNLSDVHQDLNIPIPTPP 215
D +++ +K E + + P + +KL L + NF D+ D +IPIPTPP
Sbjct: 2 DRPETIAVKCEPMTVDS-PSRPLKLVVLADL-NFNPPETDDL--DSSIPIPTPP 51
>At5g25910.1 68418.m03077 disease resistance family protein contains
leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Hcr2-5D [Lycopersicon
esculentum] gi|3894393|gb|AAC78596;
Length = 811
Score = 27.9 bits (59), Expect = 6.6
Identities = 35/116 (30%), Positives = 51/116 (43%), Gaps = 4/116 (3%)
Frame = -3
Query: 492 SASVSSCIETPKLFPSSIFGIINEIHILSKFESSTSSLMCGLTRCIRSLSGLLQGTEPFG 313
S ++S+ + F I I E+H L + ST+ + RCI +LS L G
Sbjct: 417 SVTISNNTRSNNNFTGKIPSFICELHSLILLDLSTNKFNGSIPRCIANLSTL--EVLNLG 474
Query: 312 ST*TRVPSMVELLDNSDESIFDV*H--LAHCLTSQVVLV--LECLNPDVRPINSDF 157
S+ E + S +SI D+ H LA L +V + LE LN + IN F
Sbjct: 475 KN-HLSGSIPENISTSVKSI-DIGHNQLAGKLPRSLVRISSLEVLNVESNKINDTF 528
>At3g50750.1 68416.m05554 brassinosteroid signalling positive
regulator-related contains similarity to BZR1 protein
[Arabidopsis thaliana] gi|20270971|gb|AAM18490
Length = 276
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = +3
Query: 165 NLSDVHQDLNIPIPTPPATSNNEPNAKRQRWTHRSYPATQP 287
NL+ + + P+ TPP +S N + RW ++P + P
Sbjct: 146 NLAPLRISNSAPV-TPPISSPRRSNPRLPRWQSSNFPVSAP 185
>At3g49430.1 68416.m05403 pre-mRNA splicing factor, putative strong
similarity to SP|O22315 Pre-mRNA splicing factor SF2
(SR1 protein) {Arabidopsis thaliana}
Length = 300
Score = 27.9 bits (59), Expect = 6.6
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +2
Query: 578 VAKYPHIDDYRRAVRELDEKEYLSLW 655
V Y + DD + A+R+LD+ E+ + W
Sbjct: 163 VVDYTNYDDMKYAIRKLDDTEFRNPW 188
>At1g65390.2 68414.m07419 disease resistance protein (TIR class),
putative domain signature TIR exists, suggestive of a
disease resistance protein.
Length = 297
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +3
Query: 99 KGFPEKIVKLNELLETSNFQNRNLSDVHQDLNIPIPTPPATSNNEPN 239
+ F + IVK + ++ + FQ RN +QD ++P T + PN
Sbjct: 173 RDFVKSIVKAVKKVQKNFFQRRNGEIEYQDFSVPACKLTITMHESPN 219
>At1g65390.1 68414.m07420 disease resistance protein (TIR class),
putative domain signature TIR exists, suggestive of a
disease resistance protein.
Length = 411
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +3
Query: 99 KGFPEKIVKLNELLETSNFQNRNLSDVHQDLNIPIPTPPATSNNEPN 239
+ F + IVK + ++ + FQ RN +QD ++P T + PN
Sbjct: 173 RDFVKSIVKAVKKVQKNFFQRRNGEIEYQDFSVPACKLTITMHESPN 219
>At1g44780.1 68414.m05130 expressed protein ; expression supported
by MPSS
Length = 471
Score = 27.9 bits (59), Expect = 6.6
Identities = 21/63 (33%), Positives = 31/63 (49%)
Frame = +2
Query: 191 KHSNTNTTCDVKQ*AKC*TSKMDSSELSSNSTIEGTRVYVLPNGSVPCNKPLSDLIHLVK 370
K S + +VK+ K SKM SSE +S+S EG + N V K ++ + L K
Sbjct: 206 KCSTESIVKNVKKKVKSTPSKMVSSEYNSDSDTEGN----VDNEEVAVKKTMARKVKLSK 261
Query: 371 PHI 379
P +
Sbjct: 262 PEM 264
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,548,347
Number of Sequences: 28952
Number of extensions: 308235
Number of successful extensions: 923
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 922
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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