Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0114
         (650 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal pro...   163   1e-41
SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizos...   162   3e-41
SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosacch...   162   5e-41
SPBC21.03c |||DUF55 family protein|Schizosaccharomyces pombe|chr...    30   0.33 
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb...    27   3.1  
SPBC18H10.10c |cwc16||complexed with Cdc5 protein Cwf16|Schizosa...    27   3.1  
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ...    27   3.1  
SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr ...    25   7.2  
SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces p...    25   7.2  
SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyce...    25   7.2  
SPAC11E3.13c |||1,3-beta-glucanosyltransferase |Schizosaccharomy...    25   9.5  
SPAPB8E5.04c |||phosphatidylglycerol/phosphatidylinositol transf...    25   9.5  

>SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal
           protein S4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 262

 Score =  163 bits (397), Expect = 1e-41
 Identities = 80/131 (61%), Positives = 91/131 (69%)
 Frame = +2

Query: 254 LIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKR 433
           LIKVDGKVRTD T+P GFMDV+S+EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKR
Sbjct: 69  LIKVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKR 128

Query: 434 VATGPKNVPYLVTHDGRTIRYPDPLSKSTIPSS*TLQLRRLWTSSSLSPGTCV*SREAVT 613
           V  G K VP+LVTHDGRTIRYPDPL K        L+  ++ +         V       
Sbjct: 129 VQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQVMVTGGRN 188

Query: 614 WGAVGTIVSRE 646
            G VGTIV RE
Sbjct: 189 MGRVGTIVHRE 199



 Score =  113 bits (271), Expect = 3e-26
 Identities = 49/68 (72%), Positives = 55/68 (80%)
 Frame = +3

Query: 51  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 230
           M RGPKKHLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1   MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60

Query: 231 VLKIVKQR 254
           V  I+ QR
Sbjct: 61  VKAILMQR 68


>SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein
           S4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 262

 Score =  162 bits (394), Expect = 3e-41
 Identities = 79/131 (60%), Positives = 91/131 (69%)
 Frame = +2

Query: 254 LIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKR 433
           LIKVDGKVRTD T+P GFMDV+S++KT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKR
Sbjct: 69  LIKVDGKVRTDSTFPTGFMDVISVDKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKR 128

Query: 434 VATGPKNVPYLVTHDGRTIRYPDPLSKSTIPSS*TLQLRRLWTSSSLSPGTCV*SREAVT 613
           V  G K VP+LVTHDGRTIRYPDPL K        L+  ++ +         V       
Sbjct: 129 VQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQVMVTGGRN 188

Query: 614 WGAVGTIVSRE 646
            G VGTIV RE
Sbjct: 189 MGRVGTIVHRE 199



 Score =  113 bits (271), Expect = 3e-26
 Identities = 49/68 (72%), Positives = 55/68 (80%)
 Frame = +3

Query: 51  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 230
           M RGPKKHLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1   MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60

Query: 231 VLKIVKQR 254
           V  I+ QR
Sbjct: 61  VKAILMQR 68


>SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein
           S4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 262

 Score =  162 bits (393), Expect = 5e-41
 Identities = 79/131 (60%), Positives = 91/131 (69%)
 Frame = +2

Query: 254 LIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKR 433
           LI+VDGKVRTD T+P GFMDV+S+EKT E FRL+YD+KGRFT+HRIT EEAKYKLCKVKR
Sbjct: 69  LIQVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAKYKLCKVKR 128

Query: 434 VATGPKNVPYLVTHDGRTIRYPDPLSKSTIPSS*TLQLRRLWTSSSLSPGTCV*SREAVT 613
           V  G K VP+LVTHDGRTIRYPDPL K        L+  ++ +         V       
Sbjct: 129 VQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQVMVTGGRN 188

Query: 614 WGAVGTIVSRE 646
            G VGTIV RE
Sbjct: 189 MGRVGTIVHRE 199



 Score =  113 bits (271), Expect = 3e-26
 Identities = 49/68 (72%), Positives = 55/68 (80%)
 Frame = +3

Query: 51  MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 230
           M RGPKKHLKR+ AP  W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1   MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60

Query: 231 VLKIVKQR 254
           V  I+ QR
Sbjct: 61  VKAILMQR 68


>SPBC21.03c |||DUF55 family protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 239

 Score = 29.9 bits (64), Expect = 0.33
 Identities = 20/78 (25%), Positives = 37/78 (47%)
 Frame = +2

Query: 179 PGDFPEESSEVCFDRKRSPENCETTLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIY 358
           P D    S++  +D K +P+N     + V  + + D   P    ++  + K N+L  +  
Sbjct: 92  PDDSAWNSNDPYYDPKSTPQNPRWYSVGVQSEYKLDR--PVTLREL-KMHKENQLKSMEL 148

Query: 359 DVKGRFTIHRITPEEAKY 412
             + R +I R+ PEE K+
Sbjct: 149 LNRSRLSISRVKPEEWKF 166


>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1428

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 8/15 (53%), Positives = 12/15 (80%)
 Frame = +3

Query: 510 QSQRFHPVRHCNYED 554
           ++++ HPVRH  YED
Sbjct: 5   ENEKIHPVRHSKYED 19


>SPBC18H10.10c |cwc16||complexed with Cdc5 protein
           Cwf16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 294

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 21/93 (22%), Positives = 36/93 (38%), Gaps = 1/93 (1%)
 Frame = +2

Query: 239 NCETTLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKL 418
           N +    K+   +R +  +P    +  +I +    F  +    G +   +I     K  L
Sbjct: 11  NAKRKFDKLRNVIRFEMPFPVWCNNCENIIQQGTRFNAVKKEIGSYYTTKIWSFSLKCHL 70

Query: 419 CKVK-RVATGPKNVPYLVTHDGRTIRYPDPLSK 514
           C     V T PKN  Y+V   GR    P  +++
Sbjct: 71  CSNPIDVHTDPKNTEYIVASGGRRKIEPQDINE 103


>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 3655

 Score = 26.6 bits (56), Expect = 3.1
 Identities = 15/62 (24%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
 Frame = +2

Query: 311  DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYL-VTHDGRT 487
            +V S+EK N++F + Y+V  +     +   +   K+  ++ ++   KNVP + V H    
Sbjct: 1718 NVSSLEKCNQIFSIFYEVFFQHPSTNVYANDEGIKIGALQIISFFLKNVPEITVQHQTEM 1777

Query: 488  IR 493
            ++
Sbjct: 1778 LK 1779


>SPBC3D6.03c |||tRNA endonuclease |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 678

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 11/24 (45%), Positives = 12/24 (50%)
 Frame = +1

Query: 1   NSARGLXFLSXFNTCPTWREALRS 72
           N    L  L    TCPTWR +L S
Sbjct: 405 NEGYTLDVLGTSATCPTWRRSLSS 428


>SPAC607.10 |spo3||sporulation protein Spo3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1028

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 17/63 (26%), Positives = 28/63 (44%), Gaps = 7/63 (11%)
 Frame = -2

Query: 328 LN*DNIHKSSRI--SRVSPNFPINLY-----ERCFTIFRTSFPVKAYFRRFLRKITRGKH 170
           +N D+  K SRI  +++ P   +NL      E CF  + T          F++K +   H
Sbjct: 165 INLDDNRKFSRIQKNKLDPLITVNLVPIVNGENCFNFYETQMETNFLSPEFIKKPSMVDH 224

Query: 169 SRN 161
            R+
Sbjct: 225 KRD 227


>SPAC1093.01 ||SPAC12B10.18|PPR repeat protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1261

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 12/22 (54%), Positives = 15/22 (68%)
 Frame = -2

Query: 406 SLLRGDTVDCESALNIID*TKQ 341
           S  RGDT D  +ALNI + TK+
Sbjct: 898 STRRGDTDDATTALNIFEETKR 919


>SPAC11E3.13c |||1,3-beta-glucanosyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 510

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 19/62 (30%), Positives = 32/62 (51%)
 Frame = -3

Query: 345 NSSLVFSIETTSINPAG*VGSVLTFPSTFMSVVSQFSGLRFRSKHTSDDSSGKSPGASTR 166
           +SS   S  TT+ + +   GS ++  S+ MS  + ++     S H+S  SSG S   S+ 
Sbjct: 430 SSSGSSSASTTASSSSVSSGSSISSGSSSMS--TSYTSASGSSAHSSGSSSGSSSATSSA 487

Query: 165 AT 160
           +T
Sbjct: 488 ST 489


>SPAPB8E5.04c |||phosphatidylglycerol/phosphatidylinositol transfer
           protein |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 188

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 14/40 (35%), Positives = 20/40 (50%), Gaps = 3/40 (7%)
 Frame = -3

Query: 261 FMSVVSQFSGLRFRSKHTSD---DSSGKSPGASTRATCGD 151
           F+S  S FS   F    ++D    +S K PGA+  + C D
Sbjct: 16  FISASSWFSSFSFGESKSTDLVSSTSEKIPGANPASYCAD 55


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,760,775
Number of Sequences: 5004
Number of extensions: 57476
Number of successful extensions: 169
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)