BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0108 (662 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 146 3e-36 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 82 8e-17 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 79 7e-16 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 47 3e-06 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 33 0.049 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 31 0.15 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.26 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 29 0.79 SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 27 2.4 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 27 3.2 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 4.2 SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase Nak1|Schizosacch... 26 4.2 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 7.4 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 25 7.4 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 9.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 146 bits (353), Expect = 3e-36 Identities = 104/225 (46%), Positives = 114/225 (50%), Gaps = 9/225 (4%) Frame = +3 Query: 3 PAMYVAIQAXLSLFASGRTTGIVLDSGDGVSHTVPXYEGYALPHAIXXXXXXXXXXXXYL 182 PA YVAIQA LSL+ASGRTTGIVLDSGDGV+HTVP YEGYALPHAI YL Sbjct: 130 PAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYL 189 Query: 183 MKILTERGYXFTTTAEREXVRDIKRSXATSXLTXSRRWPPLHXAAXXRSLTNXPTVRSSP 362 MKIL ERGY F+TTAERE VRDIK L + L AA SL + Sbjct: 190 MKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQE---LQTAAQSSSLEKSYELPDGQ 246 Query: 363 XETKDSVARGSLPTLDLGYGSXAXXTRQXIXPX*SATWTSVRTCTPTPYXRWYHHV---- 530 T + R P L S I AT+ S+ C Y +V Sbjct: 247 VITIGN-ERFRAPEA-LFQPSALGLENAGIH---EATYNSIMKCDVDIRKDLYGNVVMSG 301 Query: 531 -----PWNRXPYAKGNTALAPSTMKXKIXXPTXRKYSVWIXGSIL 650 P K ALAPS+MK KI P RKYSVWI GSIL Sbjct: 302 GTTMYPGIADRMQKEIQALAPSSMKVKIVAPPERKYSVWIGGSIL 346 Score = 96.3 bits (229), Expect = 3e-21 Identities = 49/86 (56%), Positives = 53/86 (61%) Frame = +2 Query: 251 QEKLXYVXLDXXQEMATXXXXXXLEKSYELXXGQVITXGNERFRCQRLSSNPRSWVWKLX 430 +EKL YV LD QE+ T LEKSYEL GQVIT GNERFR P + + Sbjct: 213 KEKLCYVALDFEQELQTAAQSSSLEKSYELPDGQVITIGNERFRAPEALFQPSALGLENA 272 Query: 431 GXHETTYXSXMKCDVDIRKDLYANTV 508 G HE TY S MKCDVDIRKDLY N V Sbjct: 273 GIHEATYNSIMKCDVDIRKDLYGNVV 298 Score = 47.6 bits (108), Expect = 2e-06 Identities = 33/67 (49%), Positives = 37/67 (55%), Gaps = 3/67 (4%) Frame = +1 Query: 385 PEALFQPSILGME--AXRXXRDNXXLHXEVRRGHP*GLVRQHRMXGGTTMYPGIDXRMQK 558 PEALFQPS LG+E N + +V L M GGTTMYPGI RMQK Sbjct: 258 PEALFQPSALGLENAGIHEATYNSIMKCDVDIRKD--LYGNVVMSGGTTMYPGIADRMQK 315 Query: 559 EIQ-LSP 576 EIQ L+P Sbjct: 316 EIQALAP 322 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 81.8 bits (193), Expect = 8e-17 Identities = 38/82 (46%), Positives = 52/82 (63%) Frame = +3 Query: 9 MYVAIQAXLSLFASGRTTGIVLDSGDGVSHTVPXYEGYALPHAIXXXXXXXXXXXXYLMK 188 +YVAIQA LSL+A G ++G+V+DSGDGV+H VP YE L H + YL+ Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLIS 191 Query: 189 ILTERGYXFTTTAEREXVRDIK 254 +L +GY F TA+ E VR++K Sbjct: 192 LLLRKGYAFNRTADFETVREMK 213 Score = 46.4 bits (105), Expect = 4e-06 Identities = 27/82 (32%), Positives = 37/82 (45%) Frame = +2 Query: 251 QEKLXYVXLDXXQEMATXXXXXXLEKSYELXXGQVITXGNERFRCQRLSSNPRSWVWKLX 430 +EKL YV D + L ++Y L G+VI G+ER+ C P + Sbjct: 213 KEKLCYVSYDLELDHKLSEETTVLMRNYTLPDGRVIKVGSERYECPECLFQPHLVGSEQP 272 Query: 431 GXHETTYXSXMKCDVDIRKDLY 496 G E + + DVDIRK LY Sbjct: 273 GLSEFIFDTIQAADVDIRKYLY 294 Score = 30.3 bits (65), Expect = 0.26 Identities = 19/61 (31%), Positives = 28/61 (45%) Frame = +1 Query: 385 PEALFQPSILGMEAXRXXRDNXXLHXEVRRGHP*GLVRQHRMXGGTTMYPGIDXRMQKEI 564 PE LFQP ++G E L R + GG++MY G+ R++KEI Sbjct: 258 PECLFQPHLVGSEQPGLSEFIFDTIQAADVDIRKYLYRAIVLSGGSSMYAGLPSRLEKEI 317 Query: 565 Q 567 + Sbjct: 318 K 318 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 78.6 bits (185), Expect = 7e-16 Identities = 36/84 (42%), Positives = 55/84 (65%) Frame = +3 Query: 3 PAMYVAIQAXLSLFASGRTTGIVLDSGDGVSHTVPXYEGYALPHAIXXXXXXXXXXXXYL 182 PA+ ++Q L+L+AS RTTGIVL+ GDG++H+VP Y+G+++P AI YL Sbjct: 135 PALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYL 194 Query: 183 MKILTERGYXFTTTAEREXVRDIK 254 L + G+ ++AE+E VR+IK Sbjct: 195 QLQLRKSGHELVSSAEKEIVREIK 218 Score = 47.2 bits (107), Expect = 2e-06 Identities = 26/86 (30%), Positives = 38/86 (44%) Frame = +2 Query: 251 QEKLXYVXLDXXQEMATXXXXXXLEKSYELXXGQVITXGNERFRCQRLSSNPRSWVWKLX 430 +EK YV D E+ + +Y+L Q IT G E F + NP + Sbjct: 218 KEKCCYVASDFRSEIESWTEHKPQIHTYQLPDNQTITLGTECFSAPEVLFNPEMMGSEAS 277 Query: 431 GXHETTYXSXMKCDVDIRKDLYANTV 508 G H + S + D+D+R LY+N V Sbjct: 278 GLHIQLFKSILLSDIDLRSTLYSNIV 303 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 46.8 bits (106), Expect = 3e-06 Identities = 26/88 (29%), Positives = 45/88 (51%), Gaps = 8/88 (9%) Frame = +3 Query: 9 MYVAIQAXLSLFASGRT--------TGIVLDSGDGVSHTVPXYEGYALPHAIXXXXXXXX 164 +Y+A+QA L+L AS + TG V+DSGDGV+H +P EGY + +I Sbjct: 155 LYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSIKTMPLAGR 214 Query: 165 XXXXYLMKILTERGYXFTTTAEREXVRD 248 ++ +L +R ++ E +++ Sbjct: 215 DVTYFVQSLLRDRNEPDSSLKTAERIKE 242 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 32.7 bits (71), Expect = 0.049 Identities = 16/42 (38%), Positives = 23/42 (54%) Frame = +3 Query: 3 PAMYVAIQAXLSLFASGRTTGIVLDSGDGVSHTVPXYEGYAL 128 PA Y+A Q + FASG+ T ++D G S Y+G+ L Sbjct: 128 PATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 31.1 bits (67), Expect = 0.15 Identities = 21/70 (30%), Positives = 27/70 (38%), Gaps = 9/70 (12%) Frame = +2 Query: 326 KSYELXXGQVITXGNERFRCQRLSSNPRSWVWKLX---------GXHETTYXSXMKCDVD 478 K +E G G ERFR + NP + G HE Y S + CD + Sbjct: 279 KPFEFPDGVTHKFGQERFRISEILFNPSFSASRSAETTPPQGSVGLHELVYQSILACDSE 338 Query: 479 IRKDLYANTV 508 +R L N V Sbjct: 339 LRSPLLNNIV 348 Score = 27.1 bits (57), Expect = 2.4 Identities = 9/18 (50%), Positives = 14/18 (77%) Frame = +1 Query: 514 GGTTMYPGIDXRMQKEIQ 567 GGT++ PG+ R+Q E+Q Sbjct: 351 GGTSLIPGLSERLQAEVQ 368 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 30.3 bits (65), Expect = 0.26 Identities = 17/51 (33%), Positives = 20/51 (39%) Frame = +1 Query: 7 PCTSPSKPFSRCSRPVVPPVSCWTPATVSPTPCPXTRDTHSPTPSCVXTXP 159 P T+P P S P VP S P+ P P P + P PS P Sbjct: 1037 PSTAPPVPIPT-STPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVP 1086 Score = 27.1 bits (57), Expect = 2.4 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 5/51 (9%) Frame = +1 Query: 4 PPCTSPSKPFSRCSRPVVPPVSCWTP-----ATVSPTPCPXTRDTHSPTPS 141 PP +P P P VPP S P A + P P P + P PS Sbjct: 1193 PPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPS 1243 Score = 26.2 bits (55), Expect = 4.2 Identities = 22/78 (28%), Positives = 26/78 (33%), Gaps = 2/78 (2%) Frame = +1 Query: 4 PPCTSPSKPFSRCSRP--VVPPVSCWTPATVSPTPCPXTRDTHSPTPSCVXTXPVAXXXX 177 PP PS +P VPPV P+ P P P P PS PV Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPA--PSGAPPVPKPSVAAPPVPVPS--GAPPVPKPSV 1147 Query: 178 XXXXXXXXAGTXSLPPPS 231 +G +P PS Sbjct: 1148 AAPPVPAPSGAPPVPKPS 1165 Score = 25.4 bits (53), Expect = 7.4 Identities = 17/54 (31%), Positives = 20/54 (37%), Gaps = 2/54 (3%) Frame = +1 Query: 4 PPCTSPSKPFSRCSRPV--VPPVSCWTPATVSPTPCPXTRDTHSPTPSCVXTXP 159 PP +PS +P VPPV P+ P P P P PS P Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPVP--PPSEAPPVPKPSVGVPPVPPPSTAPPVP 1220 Score = 25.0 bits (52), Expect = 9.7 Identities = 14/47 (29%), Positives = 18/47 (38%), Gaps = 1/47 (2%) Frame = +1 Query: 4 PPCTSPSKPFSRCSRPVVPPVSCWTPATVSP-TPCPXTRDTHSPTPS 141 PP T+P P P VP + P +P + P S PS Sbjct: 1212 PPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPRSSVPS 1258 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 28.7 bits (61), Expect = 0.79 Identities = 17/69 (24%), Positives = 31/69 (44%) Frame = +1 Query: 358 HHXKRKIPLPEALFQPSILGMEAXRXXRDNXXLHXEVRRGHP*GLVRQHRMXGGTTMYPG 537 H +I +PE +F PSI+G++ + LV + GG PG Sbjct: 590 HLNVEQIRVPEVIFSPSIVGIDQAGILEIMRSILQRHSLEEQQKLVSNVLITGGLGSLPG 649 Query: 538 IDXRMQKEI 564 ++ R+++E+ Sbjct: 650 METRIKREL 658 Score = 26.2 bits (55), Expect = 4.2 Identities = 15/65 (23%), Positives = 28/65 (43%), Gaps = 2/65 (3%) Frame = +3 Query: 3 PAMYVAIQAXLSLFASGR--TTGIVLDSGDGVSHTVPXYEGYALPHAIXXXXXXXXXXXX 176 P++ I S + + + ++GIVL+ G+ SH +P G + Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQSSS 204 Query: 177 YLMKI 191 YL+K+ Sbjct: 205 YLLKL 209 >SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1016 Score = 27.1 bits (57), Expect = 2.4 Identities = 17/52 (32%), Positives = 24/52 (46%) Frame = +1 Query: 10 CTSPSKPFSRCSRPVVPPVSCWTPATVSPTPCPXTRDTHSPTPSCVXTXPVA 165 C +PS+ C + PV + T S TP P T + +PTP+ T A Sbjct: 825 CYNPSQ--YGCDNGALGPVQS-SSTTSSITPTPTTTSSITPTPTTTSTTTTA 873 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 26.6 bits (56), Expect = 3.2 Identities = 12/32 (37%), Positives = 15/32 (46%), Gaps = 1/32 (3%) Frame = +1 Query: 40 CSRP-VVPPVSCWTPATVSPTPCPXTRDTHSP 132 C R + PPV+C TP P C + H P Sbjct: 624 CGRTRLYPPVACGTPIPDCPYLCVLPKSCHHP 655 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 26.2 bits (55), Expect = 4.2 Identities = 16/58 (27%), Positives = 21/58 (36%), Gaps = 4/58 (6%) Frame = +1 Query: 4 PPCTSPSKPFSRCSRPVVPPVSCWTP----ATVSPTPCPXTRDTHSPTPSCVXTXPVA 165 PP PS P + P +PP + P + P P P P+ PVA Sbjct: 432 PPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVA 489 Score = 25.0 bits (52), Expect = 9.7 Identities = 15/52 (28%), Positives = 23/52 (44%) Frame = +1 Query: 4 PPCTSPSKPFSRCSRPVVPPVSCWTPATVSPTPCPXTRDTHSPTPSCVXTXP 159 PP P+ P S P +PP+ + + P P P + +P PS + P Sbjct: 388 PPAPPPAIPGR--SAPALPPLGNASRTSTPPVPTPPSLPPSAP-PSLPPSAP 436 >SPBC17F3.02 |nak1|orb3, mor4|PAK-related kinase Nak1|Schizosaccharomyces pombe|chr 2|||Manual Length = 652 Score = 26.2 bits (55), Expect = 4.2 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +1 Query: 10 CTSPSKPFSRCSRPVVPPVSCWTPA 84 C+S KPF+ R +PP+S +PA Sbjct: 562 CSSTPKPFNSKLRAHLPPLSIGSPA 586 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.4 bits (53), Expect = 7.4 Identities = 17/50 (34%), Positives = 20/50 (40%), Gaps = 3/50 (6%) Frame = +1 Query: 22 SKPFSRCSRPVVPPVSCWT---PATVSPTPCPXTRDTHSPTPSCVXTXPV 162 S+ S + P PP + T PA SP P H PT S PV Sbjct: 899 SRTSSVSTLPPPPPTASMTASAPAIASPPPPKVGETYHPPTASGTRVPPV 948 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 25.4 bits (53), Expect = 7.4 Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = +1 Query: 7 PCTSPSKPFSRCSRPVVPPVSCWTPATVSPTPCPXTRDTHSPT-PSCVXTXP 159 P SPS P S +P ++P + S +P T ++SPT PS T P Sbjct: 1556 PSYSPSSPGYSTSPAYMPSSPSYSPTSPSYSP---TSPSYSPTSPSYSPTSP 1604 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 25.0 bits (52), Expect = 9.7 Identities = 21/78 (26%), Positives = 28/78 (35%), Gaps = 3/78 (3%) Frame = +1 Query: 7 PCTSPSKPFSRCSRPVVPPVSCWTPATVSPTPCPXTRDTHS---PTPSCVXTXPVAXXXX 177 P T S S P VPP S + + P + DT+S PT S T + Sbjct: 122 PPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSNPLPTTSTSCTTSTSIPPT 181 Query: 178 XXXXXXXXAGTXSLPPPS 231 T ++PP S Sbjct: 182 GGSSSLSTPITPTVPPTS 199 Score = 25.0 bits (52), Expect = 9.7 Identities = 16/46 (34%), Positives = 18/46 (39%), Gaps = 1/46 (2%) Frame = +1 Query: 7 PCTSPSKPFSRCSRPVVPPVS-CWTPATVSPTPCPXTRDTHSPTPS 141 P T S S P VPP S T + PT T SP P+ Sbjct: 179 PPTGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPT 224 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,362,438 Number of Sequences: 5004 Number of extensions: 40249 Number of successful extensions: 150 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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