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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0105
         (661 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    29   0.45 
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa...    28   1.4  
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar...    27   1.8  
SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomy...    26   4.2  
SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ...    26   4.2  
SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces p...    26   5.5  
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi...    25   9.7  
SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|...    25   9.7  

>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 29.5 bits (63), Expect = 0.45
 Identities = 22/84 (26%), Positives = 35/84 (41%), Gaps = 3/84 (3%)
 Frame = -3

Query: 563 WTPDPGACCPCIKGISNSCHQMKECEMSDHSWRTHCSWRRE*RTGALRDFQVSC---TYC 393
           W  D   C  C KG+ +S  QM           + C+         ++D+ +     +Y 
Sbjct: 40  WHKDCFCCTKCDKGLEHS-DQMLVQTSDGRPVCSSCAHTCTACRMRIKDYALMSGYDSYH 98

Query: 392 TACMTYHDFRKQFVDTPFQFVHRT 321
             C   HD RKQ +D+ F+  +RT
Sbjct: 99  RECFRCHDCRKQIIDSNFKRDNRT 122


>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
           Brl2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 680

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = -2

Query: 354 CGHSIPIRAQNSKNYDGPFTAS 289
           C H I + +Q SKN++G F +S
Sbjct: 317 CSHEINVLSQQSKNFNGVFESS 338


>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
           Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 205

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 8/21 (38%), Positives = 13/21 (61%)
 Frame = +3

Query: 378 GHTSCTICTRDLKISKCPCPL 440
           GH  C+ C ++L+    PCP+
Sbjct: 168 GHLFCSTCAKELRKKTVPCPV 188


>SPAC1F7.02c |||ATP-dependent RNA helicase Has1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 578

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = -1

Query: 604 AQRGVIVCTEYAYSGLLIPA 545
           A++G+++CT  A  GL IPA
Sbjct: 385 AEKGILLCTNVAARGLDIPA 404


>SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 791

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 8/18 (44%), Positives = 14/18 (77%)
 Frame = +1

Query: 520 IPLIHGQHAPGSGVQNTH 573
           +P ++GQ+AP +G+ N H
Sbjct: 688 VPPMYGQYAPNNGMMNMH 705


>SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 704

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 10/30 (33%), Positives = 19/30 (63%)
 Frame = +2

Query: 389 LYNMYKRPENLEVPLSATRDAKNSVYAMND 478
           L+N+  +     +P+S+T+D+K S Y+  D
Sbjct: 104 LHNLLSKAFRSTLPMSSTKDSKKSRYSSPD 133


>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 306

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 13/43 (30%), Positives = 20/43 (46%)
 Frame = +3

Query: 303 DRHNFSSSVHELEWSVHKLLPKIMIGHTSCTICTRDLKISKCP 431
           D+H  SS   E +      LP I  G+  C++C   ++   CP
Sbjct: 227 DQHTISSITDERDLEDKNKLPFIPEGNRKCSLC---MEFIHCP 266


>SPCC4G3.18 |||conserved fungal family|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 828

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 2/35 (5%)
 Frame = -1

Query: 652 LVGPSRPVLDSSCLPAAQRGVIVCT--EYAYSGLL 554
           LV P+ P   ++CL  A+ G  +CT     Y G++
Sbjct: 142 LVTPNLPTFVNNCLRIAESGKCLCTVCSCLYQGII 176


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,814,406
Number of Sequences: 5004
Number of extensions: 57242
Number of successful extensions: 166
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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