BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0079
(650 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 57 2e-09
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 39 5e-04
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 28 1.3
SPAC57A7.04c |pabp||mRNA export shuttling protein |Schizosacchar... 25 7.2
SPBC543.02c |||DNAJ/TPR domain protein DNAJC7 family|Schizosacch... 25 9.5
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 9.5
>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 446
Score = 57.2 bits (132), Expect = 2e-09
Identities = 31/77 (40%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +2
Query: 26 KILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLETDTKKKWT 205
+I P N +GKA Y+ SEN I WKI R G E AE+EL T ++ W
Sbjct: 335 RIPVPTNVVKANPRVNRGKAGYEPSENIINWKIPRFLGETELIFYAEVELSNTTNQQIWA 394
Query: 206 RPPISMGFEV-PFAPSG 253
+PPIS+ F + F SG
Sbjct: 395 KPPISLDFNILMFTSSG 411
Score = 32.7 bits (71), Expect = 0.047
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = +1
Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETR 360
+ V+YL+V EP + S + IKWVRY R+G E R
Sbjct: 412 LHVQYLRVSEP--SNSKYKSIKWVRYSTRAGTCEIR 445
>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 39.1 bits (87), Expect = 5e-04
Identities = 21/52 (40%), Positives = 28/52 (53%)
Frame = +1
Query: 190 QEEVDAPAHLHGVRSSLCTLRIKVRYLKVFEPKLNYSDHDVIKWVRYIGRSG 345
Q + P L T I+VRYLK+ EPKLNY + WVRY+ ++G
Sbjct: 371 QVQKKRPVQLKFAIPYFTTSGIQVRYLKITEPKLNY---HAMPWVRYVTQNG 419
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 27.9 bits (59), Expect = 1.3
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -3
Query: 309 VMVRVIQLRFEHLQVANLDPEG 244
+ +R I +RFEHL V+N+ P G
Sbjct: 150 INIRDIHIRFEHLPVSNVVPGG 171
>SPAC57A7.04c |pabp||mRNA export shuttling protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 653
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 1/27 (3%)
Frame = -2
Query: 301 PSNSASVRTPSGSEP*SGGC-KGNFEP 224
P+N++SV TPSG+ P S G +P
Sbjct: 63 PTNASSVATPSGTAPTSASLYVGELDP 89
>SPBC543.02c |||DNAJ/TPR domain protein DNAJC7
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 476
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +2
Query: 41 LNTSGVQLICLKGKAKYKASENA 109
LN V+ + L+GK Y + ENA
Sbjct: 172 LNPKNVEALVLRGKVMYYSGENA 194
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.0 bits (52), Expect = 9.5
Identities = 17/43 (39%), Positives = 20/43 (46%)
Frame = +2
Query: 68 CLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLETDTKK 196
C KG + + E AI WK+ MA K S ELL T K
Sbjct: 438 CQKGVEESASEEEAINWKL-LMAVSKRASRSKFAELLGYKTLK 479
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,070,343
Number of Sequences: 5004
Number of extensions: 33894
Number of successful extensions: 80
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 78
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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