BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= br--0079
(650 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g46630.2 68418.m05740 clathrin adaptor complexes medium subun... 77 1e-14
At5g46630.1 68418.m05741 clathrin adaptor complexes medium subun... 77 1e-14
At1g60780.1 68414.m06842 clathrin adaptor complexes medium subun... 44 1e-04
At1g10730.1 68414.m01223 clathrin adaptor complexes medium subun... 34 0.071
At1g72700.1 68414.m08407 haloacid dehalogenase-like hydrolase fa... 31 0.66
At3g44630.3 68416.m04798 disease resistance protein RPP1-WsB-lik... 29 2.0
At3g44630.2 68416.m04800 disease resistance protein RPP1-WsB-lik... 29 2.0
At3g44630.1 68416.m04799 disease resistance protein RPP1-WsB-lik... 29 2.0
At5g33806.1 68418.m04019 hypothetical protein 28 6.2
At4g10400.1 68417.m01707 F-box family protein contains F-box dom... 28 6.2
At3g55610.1 68416.m06177 delta 1-pyrroline-5-carboxylate synthet... 27 8.2
>At5g46630.2 68418.m05740 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 441
Score = 76.6 bits (180), Expect = 1e-14
Identities = 40/82 (48%), Positives = 50/82 (60%), Gaps = 2/82 (2%)
Frame = +2
Query: 20 LVKILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLET-DTKK 196
+VKI P T+ G+AKY S + +VWKI++ G E+ LSAEIEL+ T KK
Sbjct: 326 VVKIPVPKQTAKTNFQVTTGRAKYNPSIDCLVWKIRKFPGQTESTLSAEIELISTMGEKK 385
Query: 197 KWTRPPISMGFEVP-FAPSGSR 259
WTRPPI M F+VP F SG R
Sbjct: 386 SWTRPPIQMEFQVPMFTASGLR 407
>At5g46630.1 68418.m05741 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 438
Score = 76.6 bits (180), Expect = 1e-14
Identities = 40/82 (48%), Positives = 50/82 (60%), Gaps = 2/82 (2%)
Frame = +2
Query: 20 LVKILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIELLET-DTKK 196
+VKI P T+ G+AKY S + +VWKI++ G E+ LSAEIEL+ T KK
Sbjct: 326 VVKIPVPKQTAKTNFQVTTGRAKYNPSIDCLVWKIRKFPGQTESTLSAEIELISTMGEKK 385
Query: 197 KWTRPPISMGFEVP-FAPSGSR 259
WTRPPI M F+VP F SG R
Sbjct: 386 SWTRPPIQMEFQVPMFTASGLR 407
Score = 39.5 bits (88), Expect = 0.002
Identities = 17/37 (45%), Positives = 27/37 (72%)
Frame = +1
Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETRC 363
++VR+LKV+E S ++ ++WVRYI ++G YE RC
Sbjct: 406 LRVRFLKVWEK----SGYNTVEWVRYITKAGSYEIRC 438
>At1g60780.1 68414.m06842 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 43.6 bits (98), Expect = 1e-04
Identities = 23/75 (30%), Positives = 37/75 (49%), Gaps = 2/75 (2%)
Frame = +2
Query: 23 VKILTPLNTSGVQLICLKGKAKYKASENAIVWKIKRMAGMKETQLSAEIEL--LETDTKK 196
+++ P + S + G A Y ++A+VWKIK G KE L AE L + +
Sbjct: 315 IELPVPTDASNPTVRTSLGSASYAPEKDALVWKIKSFPGNKEYMLRAEFHLPSITAEEAT 374
Query: 197 KWTRPPISMGFEVPF 241
+ PI + FE+P+
Sbjct: 375 PERKAPIRVKFEIPY 389
Score = 33.9 bits (74), Expect = 0.094
Identities = 17/36 (47%), Positives = 22/36 (61%)
Frame = +1
Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETR 360
I+VRYLK+ E S + + WVRYI +G YE R
Sbjct: 395 IQVRYLKIIEK----SGYQALPWVRYITMAGEYELR 426
>At1g10730.1 68414.m01223 clathrin adaptor complexes medium subunit
family protein contains Pfam profile: PF00928 adaptor
complexes medium subunit family
Length = 428
Score = 34.3 bits (75), Expect = 0.071
Identities = 19/36 (52%), Positives = 22/36 (61%)
Frame = +1
Query: 253 IKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGLYETR 360
I+VRYLK+ E K Y H WVRYI +G YE R
Sbjct: 395 IQVRYLKIIE-KSGYQAHP---WVRYITMAGEYELR 426
>At1g72700.1 68414.m08407 haloacid dehalogenase-like hydrolase family
protein similar to Potential phospholipid-transporting
ATPase (EC 3.6.3.1) from Homo sapiens [SP|Q9Y2Q0,
SP|O43520]; contains InterPro accession IPR005834:
Haloacid dehalogenase-like hydrolase
Length = 1228
Score = 31.1 bits (67), Expect = 0.66
Identities = 20/67 (29%), Positives = 36/67 (53%), Gaps = 2/67 (2%)
Frame = +1
Query: 193 EEVDAPAHLHGVRSSLCTLRIKVRYLK--VFEPKLNYSDHDVIKWVRYIGRSGLYETRC* 366
+E+ APA ++ + + L T+ + Y+ F+ LN DH +I+ ++Y GR + + R
Sbjct: 1119 DEILAPAPIYWMATLLVTVAAVLPYVAHIAFQRFLNPLDHHIIQEIKYYGRD-IEDARLW 1177
Query: 367 TTRRRLA 387
T R A
Sbjct: 1178 TRERTKA 1184
>At3g44630.3 68416.m04798 disease resistance protein RPP1-WsB-like
(TIR-NBS-LRR class), putative domain signature
TIR-NBS-LRR exists, suggestive of a disease resistance
protein., closest Col-0 homolog to RPP1-WsB
Length = 1240
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
Frame = +2
Query: 26 KILTPLNTSGVQLICLKGKAKYKASE--NAIVWKIKRMAGMKETQLSAEIELLETDTKKK 199
K+ P N Q+ C+ + + E + I W++K +AG L L +K +
Sbjct: 424 KVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVKALAGKLPLGLKVLGSALRGKSKPE 483
Query: 200 WTR 208
W R
Sbjct: 484 WER 486
>At3g44630.2 68416.m04800 disease resistance protein RPP1-WsB-like
(TIR-NBS-LRR class), putative domain signature
TIR-NBS-LRR exists, suggestive of a disease resistance
protein., closest Col-0 homolog to RPP1-WsB
Length = 1214
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
Frame = +2
Query: 26 KILTPLNTSGVQLICLKGKAKYKASE--NAIVWKIKRMAGMKETQLSAEIELLETDTKKK 199
K+ P N Q+ C+ + + E + I W++K +AG L L +K +
Sbjct: 424 KVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVKALAGKLPLGLKVLGSALRGKSKPE 483
Query: 200 WTR 208
W R
Sbjct: 484 WER 486
>At3g44630.1 68416.m04799 disease resistance protein RPP1-WsB-like
(TIR-NBS-LRR class), putative domain signature
TIR-NBS-LRR exists, suggestive of a disease resistance
protein., closest Col-0 homolog to RPP1-WsB
Length = 1214
Score = 29.5 bits (63), Expect = 2.0
Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
Frame = +2
Query: 26 KILTPLNTSGVQLICLKGKAKYKASE--NAIVWKIKRMAGMKETQLSAEIELLETDTKKK 199
K+ P N Q+ C+ + + E + I W++K +AG L L +K +
Sbjct: 424 KVEYPSNDEAFQIFCMNAFGQKQPHEGFDEIAWEVKALAGKLPLGLKVLGSALRGKSKPE 483
Query: 200 WTR 208
W R
Sbjct: 484 WER 486
>At5g33806.1 68418.m04019 hypothetical protein
Length = 336
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/40 (35%), Positives = 19/40 (47%)
Frame = +3
Query: 186 TPRRSGRARPSPWGSKFPLHPPDQGSLPEGVRTEAELLGP 305
TPRR GR+ SP + P + S +G EL+ P
Sbjct: 147 TPRREGRSGRSPRRDRSPCCDSPRPSSRDGTNQRLELIAP 186
>At4g10400.1 68417.m01707 F-box family protein contains F-box domain
Pfam:PF00646
Length = 409
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +2
Query: 92 KASENAIVWKIKRMAGMKETQLSAEIELLETDTKKKWTRPPI 217
K S N +VW +K ++E L +E + E W +P I
Sbjct: 295 KYSSNLLVWFLKNSPNLRELMLVSETDDHENLGMLSWNQPSI 336
>At3g55610.1 68416.m06177 delta 1-pyrroline-5-carboxylate synthetase
B / P5CS B (P5CS2) identical to SP|P54888
Length = 726
Score = 27.5 bits (58), Expect = 8.2
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +2
Query: 80 KAKYKASENAIVWKIKRMAGMKETQLSAEIELLETDTKKKWTRPP 214
+A YK S I W +A + +L A++ +L +D + +T PP
Sbjct: 163 RAPYKDS-TGIFWDNDSLAALLSLELKADLLILLSDVEGLYTGPP 206
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,892,223
Number of Sequences: 28952
Number of extensions: 185680
Number of successful extensions: 503
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 490
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 501
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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