Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0073
         (676 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC776.02c |dis2|sds1, bws1|serine/threonine protein phosphatas...   136   4e-33
SPCC31H12.05c |sds21||serine/threonine protein phosphatase Sds21...   132   4e-32
SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Sch...   118   6e-28
SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatas...    64   2e-11
SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa...    58   1e-09
SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Sc...    53   3e-08
SPAC22H10.04 |||protein phosphatase type 2A|Schizosaccharomyces ...    50   4e-07
SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch...    48   9e-07
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc...    39   6e-04
SPBC26H8.05c |||serine/threonine protein phosphatase |Schizosacc...    36   0.004
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch...    27   2.5  
SPCC1442.11c |||sequence orphan|Schizosaccharomyces pombe|chr 3|...    26   4.3  
SPAC13G6.06c |||glycine cleavage complex subunit P|Schizosacchar...    26   5.7  
SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase [UDP-...    26   5.7  
SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5 |Schizosacch...    26   5.7  
SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr...    25   7.6  

>SPBC776.02c |dis2|sds1, bws1|serine/threonine protein phosphatase
           PP1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 327

 Score =  136 bits (328), Expect = 4e-33
 Identities = 58/69 (84%), Positives = 67/69 (97%)
 Frame = +3

Query: 48  KFLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGALMSVDETLMCSF 227
           +FL KH+ DL+CRAHQVVEDGYEFF+KRQLVTLFSAPNYCGEFDNAGA+MSVDE+L+CSF
Sbjct: 233 RFLHKHDMDLVCRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSF 292

Query: 228 QILKPADKR 254
           QILKPA+K+
Sbjct: 293 QILKPAEKK 301


>SPCC31H12.05c |sds21||serine/threonine protein phosphatase
           Sds21|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 322

 Score =  132 bits (319), Expect = 4e-32
 Identities = 57/69 (82%), Positives = 64/69 (92%)
 Frame = +3

Query: 48  KFLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGALMSVDETLMCSF 227
           +FL KH+ DLICRAHQVVEDGYEFF KRQLVT+FSAPNYCGEFDN GA+MSV+E L+CSF
Sbjct: 230 RFLQKHDLDLICRAHQVVEDGYEFFGKRQLVTIFSAPNYCGEFDNVGAMMSVNEDLLCSF 289

Query: 228 QILKPADKR 254
           QILKPA+KR
Sbjct: 290 QILKPAEKR 298


>SPAC57A7.08 |pzh1||serine/threonine protein phosphatase
           Pzh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 515

 Score =  118 bits (285), Expect = 6e-28
 Identities = 53/86 (61%), Positives = 67/86 (77%), Gaps = 1/86 (1%)
 Frame = +3

Query: 12  RSASLLFXKS-FGKFLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAG 188
           R  S +F K+   +FL+KH+FDLICRAH VVEDGYEFF  R L T+FSAPNYCGEFDN G
Sbjct: 405 RGVSFVFNKNVIRQFLAKHDFDLICRAHMVVEDGYEFFNDRTLCTVFSAPNYCGEFDNWG 464

Query: 189 ALMSVDETLMCSFQILKPADKRSCTR 266
           A+MSV+  L+CSF+++KP D+ +  R
Sbjct: 465 AVMSVNSELLCSFELIKPLDQAAIRR 490


>SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatase
           Ppe1 |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 305

 Score = 64.1 bits (149), Expect = 2e-11
 Identities = 34/76 (44%), Positives = 49/76 (64%), Gaps = 2/76 (2%)
 Frame = +3

Query: 12  RSASLLFX-KSFGKFLSKHEFDLICRAHQVVEDGYEF-FAKRQLVTLFSAPNYCGEFDNA 185
           R A  LF  K   +F   ++  LI RAHQ+V++GY++ FA + LVT++SAPNYC    N 
Sbjct: 208 RGAGWLFGSKVTTEFSQINDLTLIARAHQLVQEGYKYHFADKNLVTVWSAPNYCYRCGNV 267

Query: 186 GALMSVDETLMCSFQI 233
            ++M VDE+L   F+I
Sbjct: 268 ASVMKVDESLEPEFRI 283


>SPAC823.15 |ppa1||minor serine/threonine protein phosphatase
           Ppa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 309

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 28/73 (38%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
 Frame = +3

Query: 12  RSASLLFXKSFGK-FLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAG 188
           R A   F     + F   +  DLI RAHQ+V +GY +     +VT+FSAPNYC    N  
Sbjct: 214 RGAGYTFGPDIAEAFNHNNGLDLIARAHQLVMEGYNWTTNHNVVTIFSAPNYCYRCGNQA 273

Query: 189 ALMSVDETLMCSF 227
           A+M +D+ +  +F
Sbjct: 274 AIMGIDDHINYAF 286


>SPBC16H5.07c |ppa2||serine/threonine protein phosphatase
           Ppa2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 322

 Score = 53.2 bits (122), Expect = 3e-08
 Identities = 28/81 (34%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
 Frame = +3

Query: 12  RSASLLFXKSFGK-FLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAG 188
           R A   F +   + F   +   L  RAHQ+V +G+ +     +VT+FSAPNYC    N  
Sbjct: 227 RGAGYTFGQDISETFNHANGLSLTARAHQLVMEGFNWAHDGDVVTIFSAPNYCYRCGNQA 286

Query: 189 ALMSVDETLMCSFQILKPADK 251
           A++ VD+T+   F    PA +
Sbjct: 287 AILEVDDTMNQVFLQFDPAPR 307


>SPAC22H10.04 |||protein phosphatase type 2A|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 307

 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 22/66 (33%), Positives = 36/66 (54%)
 Frame = +3

Query: 48  KFLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGALMSVDETLMCSF 227
           KFL  +    I RAHQ+  +GY+   +++L T++SAPNYC    N  +++ +D      F
Sbjct: 222 KFLEYNNMKHILRAHQLCSEGYQILFEKKLSTVWSAPNYCYRCANLASILQIDTDQSRFF 281

Query: 228 QILKPA 245
            +   A
Sbjct: 282 NVFDAA 287


>SPBC3F6.01c |||serine/threonine protein phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 473

 Score = 48.4 bits (110), Expect = 9e-07
 Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
 Frame = +3

Query: 12  RSASLLFXKSFGK-FLSKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAG 188
           R   L F     K F   +    + R+H+V + GYE       +T+FSAPNYC    N G
Sbjct: 377 RGVGLQFGPDVSKRFCEANGLKAVIRSHEVRDQGYEVEHDGYCITVFSAPNYCDSTGNLG 436

Query: 189 ALMSVDETLMCSF 227
           A++ V E +   F
Sbjct: 437 AVIKVKEDMELDF 449


>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
           Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 554

 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 19/62 (30%), Positives = 33/62 (53%), Gaps = 6/62 (9%)
 Frame = +3

Query: 51  FLSKHEFDLICRAHQVVEDGYEFFAKRQ------LVTLFSAPNYCGEFDNAGALMSVDET 212
           FL  +    + RAH+  + GY  + K +      L+T+FSAPNY   ++N  A++  +  
Sbjct: 297 FLENNNLLSVIRAHEAQDVGYRMYRKTKTTGFPSLMTIFSAPNYLDVYNNKAAVLKYENN 356

Query: 213 LM 218
           +M
Sbjct: 357 VM 358


>SPBC26H8.05c |||serine/threonine protein phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 348

 Score = 36.3 bits (80), Expect = 0.004
 Identities = 16/42 (38%), Positives = 25/42 (59%)
 Frame = +3

Query: 126 KRQLVTLFSAPNYCGEFDNAGALMSVDETLMCSFQILKPADK 251
           K  +VT++SAPNYC    N  +++ +DE    SF+I   A +
Sbjct: 291 KGSVVTVWSAPNYCYRCGNVASILQLDENQTQSFKIFGTASQ 332



 Score = 26.6 bits (56), Expect = 3.3
 Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
 Frame = +3

Query: 12  RSASLLFXKSFGKFLSK-HEFDLICRAHQVVEDGYE 116
           R A  LF     +  ++ ++   I RAHQ+V +GY+
Sbjct: 210 RGAGFLFGADVSEVFNRANDLSFIARAHQLVMEGYK 245


>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
           Slh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1935

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = +1

Query: 259 VLGPQHGPPQHAAARTAQEQEELMAPHARRPP 354
           V+G +   P  +     Q+ EE++ PHA+R P
Sbjct: 220 VIGKKFALPAGSEREDYQKYEEIIVPHAQRAP 251


>SPCC1442.11c |||sequence orphan|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 182

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 11/31 (35%), Positives = 21/31 (67%)
 Frame = -1

Query: 256 LRLSAGFRIWNEHISVSSTDIRAPALSNSPQ 164
           + +S  F+++N+  S+SSTD +  ++SN  Q
Sbjct: 33  ITISTIFKLYNQICSLSSTDRKKTSISNKLQ 63


>SPAC13G6.06c |||glycine cleavage complex subunit
           P|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1017

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 15/39 (38%), Positives = 25/39 (64%)
 Frame = -1

Query: 250 LSAGFRIWNEHISVSSTDIRAPALSNSPQ*FGAEKSVTS 134
           L+A  R +N H+ V++TD+ A  +  SP  +GA+ +V S
Sbjct: 281 LAATARSFNMHV-VAATDLLALTILKSPGEWGADVAVGS 318


>SPAC328.03 |tps1||alpha,alpha-trehalose-phosphate synthase
           [UDP-forming]|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 11/38 (28%), Positives = 19/38 (50%)
 Frame = +1

Query: 529 NSKKKRRAEIASKRYPVTERHLNNGSCSFQLKMHSRVS 642
           ++   RR  + S R P+T +  +NG+  F +     VS
Sbjct: 14  DASNSRRLIVVSNRLPITIKRKDNGTYDFSMSSGGLVS 51


>SPAC144.06 |apl5||AP-3 adaptor complex subunit Apl5
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 834

 Score = 25.8 bits (54), Expect = 5.7
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = -3

Query: 173 LPAVVWCREECDELPLRKELVSILDDLVR 87
           LPA++WC  E  E    +E + ILD L R
Sbjct: 475 LPAIIWCLGEYAE--FIEEYLDILDALTR 501


>SPBC29A3.13 |||PWWP domain protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 359

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 12/50 (24%), Positives = 25/50 (50%)
 Frame = +1

Query: 526 INSKKKRRAEIASKRYPVTERHLNNGSCSFQLKMHSRVSEERIRLSESLP 675
           + S  +   +++ +RYP++    + G    + K   +  +ER R+S S P
Sbjct: 204 VASTSRSSTQLSDQRYPLSSNFDHRGEAKGKGKQPLKNPQERGRISPSSP 253


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,731,077
Number of Sequences: 5004
Number of extensions: 56659
Number of successful extensions: 181
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 179
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)