BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0060 (650 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23C4.04c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 28 1.3 SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 28 1.3 SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 28 1.3 SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 26 4.1 SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 26 5.4 SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 26 5.4 SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 7.2 SPCC188.03 |cnd3||condensin subunit Cnd3 |Schizosaccharomyces po... 25 7.2 SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 25 7.2 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 25 9.5 SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|ch... 25 9.5 SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 25 9.5 >SPAC23C4.04c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual Length = 104 Score = 27.9 bits (59), Expect = 1.3 Identities = 9/28 (32%), Positives = 18/28 (64%) Frame = +1 Query: 514 HIASISHDNCKLFYVIDNVVYYVSLNES 597 + S S+D+C +Y+I N+ +Y + +S Sbjct: 4 YFLSKSYDDCSRYYIISNMAFYFPVYDS 31 >SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 942 Score = 27.9 bits (59), Expect = 1.3 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = -1 Query: 227 RPVELVPSDLRTGEPVSEFVNVFKVL 150 +PV +PS L GE +S F N K+L Sbjct: 853 QPVNTIPSSLSLGEQLSAFNNFMKLL 878 >SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr 3|||Manual Length = 1647 Score = 27.9 bits (59), Expect = 1.3 Identities = 22/81 (27%), Positives = 39/81 (48%) Frame = +2 Query: 251 PPRQHYDEFASATTVASEIQGFRYLRNGTAGVHTKAGVVYYDLDTLQRSKRAVIYGDYNR 430 P Q Y ++ T VAS Q +R +R+ + + + Y L L ++ Y+R Sbjct: 746 PGVQPYVDYVVETFVASSTQ-WRLMRD-VGSIRLQYACLQYMLAVLDGLNIDLLL--YSR 801 Query: 431 YVETDDTVLLMNSNLHLFITR 493 + + L N+NLH+++TR Sbjct: 802 ILSSKVRDNLQNNNLHVYLTR 822 >SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 26.2 bits (55), Expect = 4.1 Identities = 12/42 (28%), Positives = 21/42 (50%) Frame = +3 Query: 186 LSRPQITWHELYRSLTLWRQLHLLDSTTTNSRQQPRWPAKSR 311 L R +W+ + R ++W L+L + T + R+ R K R Sbjct: 34 LERVSRSWNSIVRRSSVWHNLYLSEFGTKHLRRHGRIEKKRR 75 >SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosaccharomyces pombe|chr 3|||Manual Length = 1213 Score = 25.8 bits (54), Expect = 5.4 Identities = 11/42 (26%), Positives = 21/42 (50%) Frame = +3 Query: 201 ITWHELYRSLTLWRQLHLLDSTTTNSRQQPRWPAKSRASDIY 326 + W E+ L L LL +T N+++ W +++ +IY Sbjct: 405 VVWEEVIIMLPPHVTLILLSATVPNTKEFASWVGRTKKKNIY 446 >SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 732 Score = 25.8 bits (54), Expect = 5.4 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +1 Query: 427 PLCRNRRHSTPDE*QSTFVHNKKTN 501 P N+ H+ E +++ HNKKTN Sbjct: 6 PNLNNKEHNRASEKKNSRTHNKKTN 30 >SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharomyces pombe|chr 1|||Manual Length = 671 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 6/34 (17%) Frame = +2 Query: 374 DLDTLQRSKRAVI------YGDYNRYVETDDTVL 457 +LDTL KR + + DY Y ETDD V+ Sbjct: 545 ELDTLLNGKRDIDVQDWKRFTDYRSYTETDDIVI 578 >SPCC188.03 |cnd3||condensin subunit Cnd3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 875 Score = 25.4 bits (53), Expect = 7.2 Identities = 9/29 (31%), Positives = 15/29 (51%) Frame = +2 Query: 422 YNRYVETDDTVLLMNSNLHLFITRKLIRS 508 Y R +T D + +N N+HL + + S Sbjct: 724 YERKTQTGDDYIALNHNVHLHLANMIFES 752 >SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizosaccharomyces pombe|chr 1|||Manual Length = 506 Score = 25.4 bits (53), Expect = 7.2 Identities = 9/34 (26%), Positives = 20/34 (58%) Frame = +3 Query: 33 IFTFLPAKQLTKCREVCIRWKNVIDTLNKYHSLW 134 +F++L L KC+ + RWK +++ + +L+ Sbjct: 84 VFSYLDQLDLCKCKLMSKRWKRLLEDPGIWKALY 117 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +3 Query: 213 ELYRSLTLWRQLHLLDSTTTNSRQQPRWPAKSRAS 317 E Y+S +WR+ + TTNS R A+ AS Sbjct: 964 EAYKSAGMWRECLSILQQTTNSEDLIRETAEDLAS 998 >SPCC18.04 |pof6||F-box protein Pof6|Schizosaccharomyces pombe|chr 3|||Manual Length = 872 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +3 Query: 33 IFTFLPAKQLTKCREVCIRWKNVIDTLNKYHSLWYK 140 IFT + L CR VC +++ + D Y+S++ K Sbjct: 41 IFTLISTPDLCNCRLVCRKFQQLCD----YNSIYVK 72 >SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pombe|chr 2|||Manual Length = 662 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/43 (30%), Positives = 22/43 (51%), Gaps = 1/43 (2%) Frame = +3 Query: 24 LSXIFTFLPAKQLTKCREVCIRWKNVI-DTLNKYHSLWYKFCG 149 L IF+FL + L + C WK ++ D L+ + ++ F G Sbjct: 39 LIIIFSFLDPRSLLSAQCTCKYWKKLLSDDLSWRTAFFHHFAG 81 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,598,113 Number of Sequences: 5004 Number of extensions: 52254 Number of successful extensions: 166 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 161 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 166 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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