BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0054 (659 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_566| Best HMM Match : Keratin_B2 (HMM E-Value=9) 56 3e-08 SB_43151| Best HMM Match : No HMM Matches (HMM E-Value=.) 36 0.039 SB_18916| Best HMM Match : GPS (HMM E-Value=1e-17) 31 0.83 SB_24101| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.5 >SB_566| Best HMM Match : Keratin_B2 (HMM E-Value=9) Length = 258 Score = 55.6 bits (128), Expect = 3e-08 Identities = 25/45 (55%), Positives = 32/45 (71%), Gaps = 1/45 (2%) Frame = +2 Query: 8 HEDYLVPXXMLXKAN-IFDICYADSNRSCCFTKAYTHYVEGTGSV 139 +E +L+P +L + + + DI A S RSCCFTKAY HY EGTGSV Sbjct: 207 YEQFLLPTKVLSRFSLVLDIVTAKSRRSCCFTKAYGHYAEGTGSV 251 >SB_43151| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1719 Score = 35.5 bits (78), Expect = 0.039 Identities = 20/50 (40%), Positives = 28/50 (56%) Frame = +1 Query: 247 VL*SKEILQLMSFPSEYSFPKTVTRKQCYRLLGNSVNVKVISELLQILFD 396 +L E+ +L FPS Y+ + R Q RLLGN+ +V V+ LL L D Sbjct: 1665 ILWCTEMERLFGFPSHYTDVNNMGRTQRQRLLGNAWSVPVVRHLLSPLKD 1714 >SB_18916| Best HMM Match : GPS (HMM E-Value=1e-17) Length = 1470 Score = 31.1 bits (67), Expect = 0.83 Identities = 11/28 (39%), Positives = 19/28 (67%) Frame = -2 Query: 652 KRSCNVISLNVEHVTCTPCAYDSTLTLL 569 +R C+++ N+ H TCT C++ ST L+ Sbjct: 1437 RRGCSIVESNLTHTTCT-CSHMSTFALI 1463 >SB_24101| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 519 Score = 30.3 bits (65), Expect = 1.5 Identities = 18/60 (30%), Positives = 31/60 (51%) Frame = -2 Query: 586 STLTLLNFFFV*EIRKNIASNNM*LKILTLH*STIINKMLLLSFQFKYKKKLQYIYRNYY 407 S L L + FF+ E+ A+N+M L + H + + +++ F +Y KK Q RN + Sbjct: 20 SMLALTSSFFLVELIVGYATNSMALVADSFHMLSDVMSLIIGYFALRYSKKSQRTERNTF 79 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,117,782 Number of Sequences: 59808 Number of extensions: 303368 Number of successful extensions: 502 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 437 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 502 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1693527500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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