BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0023 (663 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F12.02c |p23fy||translationally controlled tumor protein ho... 69 5e-13 SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 30 0.26 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 29 0.45 SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 26 5.6 SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 26 5.6 SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyce... 25 7.4 SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 25 9.7 >SPAC1F12.02c |p23fy||translationally controlled tumor protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 69.3 bits (162), Expect = 5e-13 Identities = 32/66 (48%), Positives = 43/66 (65%) Frame = -2 Query: 446 KKSYTLYLKDYMKKLVAKLEEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDG 267 KKSY Y+K YMK + A+L+E P++V VF+ N +K IL FK+ F+ GESMD D Sbjct: 81 KKSYMSYIKGYMKAIKARLQESNPERVPVFEKNAIGFVKKILANFKDYDFYIGESMDPDA 140 Query: 266 MVAMWN 249 MV + N Sbjct: 141 MVVLMN 146 Score = 52.4 bits (120), Expect = 6e-08 Identities = 25/54 (46%), Positives = 37/54 (68%), Gaps = 1/54 (1%) Frame = -1 Query: 663 GDEMFSDTYKMKLVDEVIYEVTGRLVTRAQG-DIQIEGFNPSAEEADEGTDSAS 505 GDE+ SD Y +K VD+++YE ++VT QG D+ I G NPSAE+A+E + + Sbjct: 10 GDELVSDAYDLKEVDDIVYEADCQMVTVKQGGDVDI-GANPSAEDAEENAEEGT 62 Score = 26.2 bits (55), Expect = 4.2 Identities = 14/26 (53%), Positives = 17/26 (65%) Frame = -1 Query: 255 VEYRDFDGTQIPIMMFFKHGLEEEKF 178 + YR+ DG P M+FFK GL EKF Sbjct: 145 MNYRE-DGIT-PYMIFFKDGLVSEKF 168 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 30.3 bits (65), Expect = 0.26 Identities = 17/40 (42%), Positives = 23/40 (57%) Frame = +2 Query: 137 ASLELLSYFLSNI*NFSSSRPCLKNIMIGICVPSKSLYST 256 AS ELL+ NFS+ R CL+N ++ CVP +Y T Sbjct: 781 ASFELLNNLTEARKNFSNYRDCLENCVLP-CVPFLGVYFT 819 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 29.5 bits (63), Expect = 0.45 Identities = 13/49 (26%), Positives = 24/49 (48%) Frame = -2 Query: 389 EEKAPDQVEVFKTNMNKVMKDILGRFKELQFFTGESMDCDGMVAMWNIE 243 E+ +Q E K+ + K++ EL+ E++D DG + N+E Sbjct: 1479 EQITKEQFEQLKSEKERTEKELADSKNELEHLQSEAVDADGKTEISNLE 1527 >SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase Gpt1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1448 Score = 25.8 bits (54), Expect = 5.6 Identities = 12/27 (44%), Positives = 18/27 (66%), Gaps = 1/27 (3%) Frame = +3 Query: 471 SLWFRTM-STPLSTPSPCPRQPLQPKD 548 S + +T+ STP+ST SP + L P+D Sbjct: 844 SSYLKTLESTPMSTSSPTKEEKLFPRD 870 >SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 987 Score = 25.8 bits (54), Expect = 5.6 Identities = 15/39 (38%), Positives = 18/39 (46%) Frame = +3 Query: 447 APKAYVSTSLWFRTMSTPLSTPSPCPRQPLQPKD*NPRS 563 +P Y+ S R ST T P PR PL P N R+ Sbjct: 87 SPFPYLKGSFDDRFSSTHSLTRQPSPRSPLTPLKGNTRA 125 >SPAC22A12.09c |sap114||splicing factor Sap114|Schizosaccharomyces pombe|chr 1|||Manual Length = 481 Score = 25.4 bits (53), Expect = 7.4 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +3 Query: 297 ELKFLKPAEDVFHYFVHVCFKYFNLVR 377 + FLKP ++ YF+ + +Y +L+R Sbjct: 175 QFDFLKPNNALYPYFMRIVQQYTSLIR 201 >SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2670 Score = 25.0 bits (52), Expect = 9.7 Identities = 9/28 (32%), Positives = 16/28 (57%) Frame = -2 Query: 449 CKKSYTLYLKDYMKKLVAKLEEKAPDQV 366 CK Y Y+ D+++ + E+K+ D V Sbjct: 2165 CKLDYYRYVGDWVRHFITLFEDKSQDVV 2192 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,725,707 Number of Sequences: 5004 Number of extensions: 54729 Number of successful extensions: 165 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 159 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 165 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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