BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= br--0004 (596 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 112 4e-26 SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 86 3e-18 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 26 4.8 SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces ... 25 6.3 SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 25 6.3 SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual 25 6.3 SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces ... 25 6.3 SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein Pop1... 25 8.4 SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz... 25 8.4 SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 25 8.4 >SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 112 bits (269), Expect = 4e-26 Identities = 49/84 (58%), Positives = 62/84 (73%) Frame = +3 Query: 258 GKIDIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPF 437 GKI IG+DVA+SEF+ DGKYDLD K P + L+ +L D+Y++ K +P+VSIEDPF Sbjct: 239 GKIKIGLDVASSEFYVDGKYDLDIKAAKPKPENKLTYQQLTDLYVELSKKYPIVSIEDPF 298 Query: 438 DQDDWSAWANLTGRTPIQIVGDDL 509 DQDDWSAW ++ T QIVGDDL Sbjct: 299 DQDDWSAWTHMKAETDFQIVGDDL 322 Score = 93.1 bits (221), Expect = 3e-20 Identities = 44/73 (60%), Positives = 55/73 (75%) Frame = +1 Query: 34 LAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKD 213 LA QEFMI PTGA +FSEAMR G+E YH LK I K+++G + VGDEGG AP++Q ++ Sbjct: 164 LAFQEFMILPTGAPSFSEAMRWGAETYHTLKSIAKKRYGSSAGNVGDEGGIAPDLQTPQE 223 Query: 214 ALYLIQDAIQKAG 252 AL LI +AI KAG Sbjct: 224 ALDLIVEAINKAG 236 Score = 46.4 bits (105), Expect = 3e-06 Identities = 21/29 (72%), Positives = 24/29 (82%) Frame = +2 Query: 509 TVTNPKRIATAVEKKACNCLLLKVNQIGS 595 TVTN KR+ TA++KK N LLLKVNQIGS Sbjct: 323 TVTNVKRLRTAIDKKCANALLLKVNQIGS 351 >SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 86.2 bits (204), Expect = 3e-18 Identities = 40/73 (54%), Positives = 53/73 (72%) Frame = +1 Query: 34 LAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKD 213 LA QEFMI P A TFSE +R GSEVYH LK + K+K+G + VGDEGG AP++ ++ Sbjct: 165 LAFQEFMIMPIKAPTFSEGLRWGSEVYHTLKALAKKKYGASAGNVGDEGGIAPDLTTAEE 224 Query: 214 ALYLIQDAIQKAG 252 AL L+ +AI++AG Sbjct: 225 ALDLVNEAIKEAG 237 Score = 85.4 bits (202), Expect = 6e-18 Identities = 37/89 (41%), Positives = 57/89 (64%) Frame = +3 Query: 243 ESWLAGKIDIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVS 422 E+ GK+ IG DVAASE + YDLDFK+ P + L KL + Y I+ +P+V Sbjct: 235 EAGYDGKVKIGFDVAASELYNGKLYDLDFKSEHPKPENKLDYKKLYEKYSALIEKYPIVF 294 Query: 423 IEDPFDQDDWSAWANLTGRTPIQIVGDDL 509 IEDPF ++DW A++ ++ +T ++++ DDL Sbjct: 295 IEDPFSEEDWGAFSYMSSKTKVEVIADDL 323 Score = 41.5 bits (93), Expect = 9e-05 Identities = 18/29 (62%), Positives = 23/29 (79%) Frame = +2 Query: 509 TVTNPKRIATAVEKKACNCLLLKVNQIGS 595 TVTN KR++ A+E K N LL+K+NQIGS Sbjct: 324 TVTNVKRLSKAIELKCANALLVKINQIGS 352 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 25.8 bits (54), Expect = 4.8 Identities = 12/44 (27%), Positives = 23/44 (52%) Frame = -3 Query: 213 ILVVLYVWSETTFITNSRRVQSKLLLNDLFQMVVHF*THPHGFT 82 ++ V +++S+TT I + + K L+NDL ++ FT Sbjct: 551 LITVCHIFSDTTLIKPVKELFQKYLVNDLPSSILKLGPQSPAFT 594 >SPBC106.04 |ada1||adenosine deaminase Ada1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 846 Score = 25.4 bits (53), Expect = 6.3 Identities = 19/54 (35%), Positives = 28/54 (51%), Gaps = 15/54 (27%) Frame = +3 Query: 336 PDSNPG-----DYLSSDKLADVYLDFIKDFPM------VSIE----DPFDQDDW 452 P+++PG D L SD+LA++YL K M VS++ +P D D W Sbjct: 154 PETDPGNMETTDPLFSDELAEIYLSIHKCMDMRHKYIRVSLQGELDNPIDDDSW 207 >SPAC1565.07c |||TATA binding protein interacting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1220 Score = 25.4 bits (53), Expect = 6.3 Identities = 15/64 (23%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Frame = +3 Query: 258 GKIDIG-MDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDP 434 G++D G + + A+E+F +L+ N D ++ L FIK+ + + D Sbjct: 841 GQLDYGKLTLPANEYFDTIASNLNSPNADVMKAAAIALGSLTSQSEKFIKELCALYVSDA 900 Query: 435 FDQD 446 +D++ Sbjct: 901 YDKE 904 >SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 723 Score = 25.4 bits (53), Expect = 6.3 Identities = 17/73 (23%), Positives = 34/73 (46%) Frame = +3 Query: 234 CYPESWLAGKIDIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFP 413 C P S + + I +++A+ FKD ++F+ S + + AD+ D + FP Sbjct: 163 CVPYSCFSNQQPIYIEIASQAIFKD--RSINFELAISQSQHSIRAMSAADI--DTLHAFP 218 Query: 414 MVSIEDPFDQDDW 452 + +E ++ W Sbjct: 219 ALHVEHQTPENLW 231 >SPAC26H5.11 |||spore wall assembly protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 965 Score = 25.4 bits (53), Expect = 6.3 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -2 Query: 301 KNSEAATSMPMSILPASQLSG*HPESDKEH 212 ++S+ + + I P SQLS HP S + H Sbjct: 184 RDSQNSLEIAPLISPESQLSSLHPSSSRRH 213 >SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein Pop1|Schizosaccharomyces pombe|chr 2|||Manual Length = 775 Score = 25.0 bits (52), Expect = 8.4 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = +3 Query: 315 YDLDFKNPDSNPGDYLSSDKLA 380 YD +NP +P ++LSS K A Sbjct: 229 YDFSSENPSIHPSNHLSSQKNA 250 >SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 900 Score = 25.0 bits (52), Expect = 8.4 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = -3 Query: 231 LNQIKSILVVLYVWSETTFITNSRRVQSKLLLNDLFQMVV 112 LNQ K +L LY+W + ++ ++Q LL+N L ++++ Sbjct: 443 LNQ-KDVLR-LYLWKKLRSYKSTMKIQKSLLVNWLLELML 480 >SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 2386 Score = 25.0 bits (52), Expect = 8.4 Identities = 14/42 (33%), Positives = 21/42 (50%), Gaps = 4/42 (9%) Frame = +3 Query: 300 FKDGKYDLDFKNPDSNPGDYLSS---DKLADVYLD-FIKDFP 413 +++ K DLDF NPGD K V+ + F++ FP Sbjct: 2159 YQEIKVDLDFALRSPNPGDIFEKKILPKFPPVFYEWFVESFP 2200 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,646,370 Number of Sequences: 5004 Number of extensions: 56231 Number of successful extensions: 159 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 159 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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