Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= br--0004
         (596 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot...   124   6e-29
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ...   114   4e-26
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S...    81   7e-16
At3g17740.1 68416.m02264 expressed protein                             29   2.4  
At5g16290.2 68418.m01904 acetolactate synthase small subunit, pu...    28   5.4  
At5g16290.1 68418.m01903 acetolactate synthase small subunit, pu...    28   5.4  
At4g33945.1 68417.m04817 armadillo/beta-catenin repeat family pr...    28   5.4  
At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q1...    27   7.2  
At3g58400.1 68416.m06509 meprin and TRAF homology domain-contain...    27   9.5  

>At1g74030.1 68414.m08573 enolase, putative similar to
           Swiss-Prot:P15007 enolase (EC 4.2.1.11)
           (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
           hydro-lyase) [Drosophila melanogaster]
          Length = 477

 Score =  124 bits (298), Expect = 6e-29
 Identities = 57/85 (67%), Positives = 69/85 (81%), Gaps = 1/85 (1%)
 Frame = +3

Query: 258 GKIDIGMDVAASEFF-KDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDP 434
           GKI IGMDVAASEFF KDG+YDL+FK   ++    LS++ LAD+Y +FIKDFP+VSIEDP
Sbjct: 283 GKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDP 342

Query: 435 FDQDDWSAWANLTGRTPIQIVGDDL 509
           FDQDDWS+WA+L     IQ+VGDDL
Sbjct: 343 FDQDDWSSWASLQSSVDIQLVGDDL 367



 Score =  109 bits (261), Expect = 2e-24
 Identities = 50/73 (68%), Positives = 61/73 (83%)
 Frame = +1

Query: 34  LAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKD 213
           LAMQEFMI P GA++FSEA +MGSEVYH LK IIK K+G D+  VGDEGGFAPN+Q+N++
Sbjct: 208 LAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNRE 267

Query: 214 ALYLIQDAIQKAG 252
            L L+ DAI+KAG
Sbjct: 268 GLVLLIDAIEKAG 280



 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 21/28 (75%), Positives = 26/28 (92%)
 Frame = +2

Query: 512 VTNPKRIATAVEKKACNCLLLKVNQIGS 595
           VTNPKRIA A++K++CN LLLKVNQIG+
Sbjct: 369 VTNPKRIAEAIKKQSCNALLLKVNQIGT 396


>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
           enolase (EC 4.2.1.11) (2-phosphoglycerate
           dehydratase)(2-phospho-D- glycerate hydro-lyase)
           [Arabidopsis thaliana]
          Length = 444

 Score =  114 bits (275), Expect = 4e-26
 Identities = 51/73 (69%), Positives = 62/73 (84%)
 Frame = +1

Query: 34  LAMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKD 213
           LAMQEFMI P GA++F EAM+MG EVYHHLK +IK+K+G D+T VGDEGGFAPNIQ NK+
Sbjct: 168 LAMQEFMILPVGAASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKE 227

Query: 214 ALYLIQDAIQKAG 252
            L L++ AI+KAG
Sbjct: 228 GLELLKTAIEKAG 240



 Score =  102 bits (244), Expect = 2e-22
 Identities = 48/87 (55%), Positives = 63/87 (72%), Gaps = 3/87 (3%)
 Frame = +3

Query: 258 GKIDIGMDVAASEFFKDGK-YDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDP 434
           GK+ IGMDVAASEF+ + K YDL+FK  ++N    +S D L D+Y  F+ ++P+VSIEDP
Sbjct: 243 GKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDP 302

Query: 435 FDQDDWSAWANLTGR--TPIQIVGDDL 509
           FDQDDW  +A +T    T +QIVGDDL
Sbjct: 303 FDQDDWEHYAKMTTECGTEVQIVGDDL 329



 Score = 49.2 bits (112), Expect = 2e-06
 Identities = 21/28 (75%), Positives = 25/28 (89%)
 Frame = +2

Query: 512 VTNPKRIATAVEKKACNCLLLKVNQIGS 595
           VTNPKR+A A+ +K+CN LLLKVNQIGS
Sbjct: 331 VTNPKRVAKAIAEKSCNALLLKVNQIGS 358


>At2g29560.1 68415.m03590 enolase, putative similar to enolase
           [Spinacia oleracea] gi|8919731|emb|CAB96173
          Length = 475

 Score = 80.6 bits (190), Expect = 7e-16
 Identities = 37/72 (51%), Positives = 50/72 (69%)
 Frame = +1

Query: 37  AMQEFMIFPTGASTFSEAMRMGSEVYHHLKKIIKEKFGLDSTAVGDEGGFAPNIQNNKDA 216
           A+QE MI P GAS F EA++ GSE YHHLK +I EK G     VG++GG AP+I + K+ 
Sbjct: 206 AIQEIMILPIGASRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGLAPDISSLKEG 265

Query: 217 LYLIQDAIQKAG 252
           L L+++AI + G
Sbjct: 266 LELVKEAINRTG 277



 Score = 80.6 bits (190), Expect = 7e-16
 Identities = 35/83 (42%), Positives = 54/83 (65%)
 Frame = +3

Query: 261 KIDIGMDVAASEFFKDGKYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPFD 440
           KI I +D+AA+ F    KYDLD K+P+ +  ++ S++ + D+Y +   D+P+VSIEDPFD
Sbjct: 281 KIKIAIDIAATNFCLGTKYDLDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFD 340

Query: 441 QDDWSAWANLTGRTPIQIVGDDL 509
           ++DW      +     Q+VGDDL
Sbjct: 341 KEDWEHTKYFSSLGICQVVGDDL 363



 Score = 39.1 bits (87), Expect = 0.002
 Identities = 15/28 (53%), Positives = 23/28 (82%)
 Frame = +2

Query: 512 VTNPKRIATAVEKKACNCLLLKVNQIGS 595
           ++N KR+  A+++ +CN LLLKVNQIG+
Sbjct: 365 MSNSKRVERAIQESSCNALLLKVNQIGT 392


>At3g17740.1 68416.m02264 expressed protein
          Length = 1149

 Score = 29.1 bits (62), Expect = 2.4
 Identities = 23/80 (28%), Positives = 37/80 (46%), Gaps = 7/80 (8%)
 Frame = +3

Query: 324 DFKNPDSNPGDYLSSDKLA-----DVYLDFIKDFPMVSIEDPFDQDDWSAWANLTGRTPI 488
           D   P+S+  D LS D +      D  L+  ++F  V+ E P D   W A+A+   +   
Sbjct: 248 DVTVPESDEEDVLSKDSMIGASWEDEVLNKTREFNRVTRERPHDAKAWLAFADFQDKVSS 307

Query: 489 QIVGDDLR*QTL--SVSLLQ 542
                 +R QTL   +S+L+
Sbjct: 308 MQSQKGVRLQTLEKKISILE 327


>At5g16290.2 68418.m01904 acetolactate synthase small subunit,
           putative similar to gi:5931761 from Nicotiana
           plumbaginifolia
          Length = 477

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 12/36 (33%), Positives = 19/36 (52%)
 Frame = -1

Query: 503 ITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 396
           +T N N  +  +V P  P    K +L+ H G ++DE
Sbjct: 265 LTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300


>At5g16290.1 68418.m01903 acetolactate synthase small subunit,
           putative similar to gi:5931761 from Nicotiana
           plumbaginifolia
          Length = 477

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 12/36 (33%), Positives = 19/36 (52%)
 Frame = -1

Query: 503 ITNNLNRRATSEVSPCRPIILVKRILNGHHGKIFDE 396
           +T N N  +  +V P  P    K +L+ H G ++DE
Sbjct: 265 LTGNGNASSGGDVYPVEPYNDFKPVLDAHWGMVYDE 300


>At4g33945.1 68417.m04817 armadillo/beta-catenin repeat family
           protein contains Pfam profile: PF00514
           armadillo/beta-catenin-like repeat
          Length = 464

 Score = 27.9 bits (59), Expect = 5.4
 Identities = 21/62 (33%), Positives = 28/62 (45%), Gaps = 6/62 (9%)
 Frame = +3

Query: 267 DIGMDVAASEFFKDGKYDLDFKNPD------SNPGDYLSSDKLADVYLDFIKDFPMVSIE 428
           D+GMD   SE  +D  Y L  +  D        PG+    D      LD +K+F  VSI+
Sbjct: 21  DLGMD--PSEALEDALYTLKLQGVDLFGIITCVPGESSVKDNPVIACLDRLKEFDSVSID 78

Query: 429 DP 434
            P
Sbjct: 79  GP 80


>At1g26370.1 68414.m03217 RNA helicase, putative similar to
           SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
           HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
           profiles PF04408: Helicase associated domain (HA2),
           PF00271: Helicase conserved C-terminal domain
          Length = 717

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 15/36 (41%), Positives = 21/36 (58%)
 Frame = -2

Query: 346 LESGFLKSRSYFPSLKNSEAATSMPMSILPASQLSG 239
           ++ GF+K+RSY PS K  E+   +P S     Q SG
Sbjct: 373 IDPGFVKARSYDPS-KGMESLDVVPASKAQTLQRSG 407


>At3g58400.1 68416.m06509 meprin and TRAF homology domain-containing
           protein / MATH domain-containing protein weak similarity
           to ubiquitin-specific protease 12 [Arabidopsis thaliana]
           GI:11993471; contains Pfam PF00917: MATH domain
          Length = 316

 Score = 27.1 bits (57), Expect = 9.5
 Identities = 12/42 (28%), Positives = 21/42 (50%)
 Frame = +3

Query: 312 KYDLDFKNPDSNPGDYLSSDKLADVYLDFIKDFPMVSIEDPF 437
           +YD D+  PD+   DY + ++ ++   D+  D P   I   F
Sbjct: 13  EYDSDYSYPDNYMSDYSAYEEESEPEFDYGDDSPFEGIPASF 54


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,135,167
Number of Sequences: 28952
Number of extensions: 305590
Number of successful extensions: 791
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 764
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 787
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)