Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte8l08
(642 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 72 8e-14
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 48 1e-06
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 42 6e-05
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 34 0.020
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 28 1.3
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 27 2.3
SPAC2G11.13 |atg22||autophagy associated protein Atg22 |Schizosa... 27 3.0
SPACUNK4.11c |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 5.3
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 25 7.0
SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 7.0
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 25 7.0
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 71.7 bits (168), Expect = 8e-14
Identities = 55/197 (27%), Positives = 89/197 (45%), Gaps = 2/197 (1%)
Frame = +2
Query: 56 LSNQDIVPCILPFIHIYGLVISLMGHLSKGCKLVTMSKFSANLFFDVLKNQDVSLLYIVP 235
L +D ++P H++GL+ L+ L+ G V KFSA+ F+ + VP
Sbjct: 199 LDPRDTSYVVMPLFHVHGLLCGLLSTLASGGCAVVPPKFSAHSFWKEFIQYGATWYTAVP 258
Query: 236 PV-AILLGKHPDVQAEHFRSVRNIICGAAPLAASDVDAILEKSKKNIEFNQGFGATETT- 409
+ ILL P R +R+ AP S ++A + + TE +
Sbjct: 259 TIHQILLRTPPPKPLPRIRFIRSCSSPLAPPVLSKLEATFRAPVL-----EAYAMTEASH 313
Query: 410 SLGTSTFKGSTNVDYSACGMPMVNFKLKFADPVTGEPIPIGEQGEMYMKSPTVMKGYHKN 589
+ T+ + +S G P +LK D G +P G++GE+ ++ V KGY N
Sbjct: 314 QMTTNPLPPLVHKPHSV-GKPF-GVELKILDQ-KGNEMPQGKEGEICVRGINVTKGYLNN 370
Query: 590 PAATKDTITEDGYFKTG 640
PAA K + T+D +F+TG
Sbjct: 371 PAANKSSFTKDRFFRTG 387
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 48.0 bits (109), Expect = 1e-06
Identities = 38/118 (32%), Positives = 53/118 (44%), Gaps = 2/118 (1%)
Frame = +2
Query: 293 VRNIICGAAPLAASDVDAILEKSKKNIEFNQGFGATETTSLGTSTFKGSTNVDYSACGMP 472
+R I G A LAAS A L S G+G TET + ++ + G P
Sbjct: 410 LRYCISGGAALAAS-TQAFL--SSCICPVLPGYGLTETCAGSFVLSPEQWHLYANTVGFP 466
Query: 473 MVNFKLKFAD-PVTGEPIPIGE-QGEMYMKSPTVMKGYHKNPAATKDTITEDGYFKTG 640
+ + + K D P G +GE++++ P V GY P K TEDG+FKTG
Sbjct: 467 IPSIEFKLVDIPDLGYYTDSSPPRGEVWIRGPAVCNGYLNRPEDNKAAFTEDGWFKTG 524
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 42.3 bits (95), Expect = 6e-05
Identities = 25/87 (28%), Positives = 44/87 (50%), Gaps = 2/87 (2%)
Frame = +2
Query: 386 GFGATETTSLGTSTFKGSTNVDYSACGMPMVNFKLKFADPVTGEPIPIGE--QGEMYMKS 559
G+G TE ++ N+D SA G + ++K D G G +GE++++
Sbjct: 432 GYGLTEISAAAMVQNPACFNLDDSA-GSLLPCTEMKLVDCEEGNYNSHGHPPRGEIWLRG 490
Query: 560 PTVMKGYHKNPAATKDTITEDGYFKTG 640
P++ +GY K++ T DG+F+TG
Sbjct: 491 PSLTRGYLNRDKENKESFTPDGWFRTG 517
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 33.9 bits (74), Expect = 0.020
Identities = 25/72 (34%), Positives = 30/72 (41%), Gaps = 1/72 (1%)
Frame = +2
Query: 389 FGATETTSLGTSTF-KGSTNVDYSACGMPMVNFKLKFADPVTGEPIPIGEQGEMYMKSPT 565
+G TE T +G S F K N S G P N T P+ +G GE+ M
Sbjct: 3011 WGPTELT-IGASAFRKVPKNAKVSNIGPPFPNCSTYILSKETKVPVLLGGLGEICMGGNQ 3069
Query: 566 VMKGYHKNPAAT 601
V KGY P T
Sbjct: 3070 VAKGYLNLPEQT 3081
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 27.9 bits (59), Expect = 1.3
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +2
Query: 464 GMPMVNFKLKFADPVTGEPIPIGEQGEMYMKS 559
GM V+ +L +P T E +GE GE++M+S
Sbjct: 1270 GMVPVSTQLAIVNPDTRELCRVGEYGEIWMRS 1301
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 27.1 bits (57), Expect = 2.3
Identities = 24/72 (33%), Positives = 31/72 (43%)
Frame = +2
Query: 257 KHPDVQAEHFRSVRNIICGAAPLAASDVDAILEKSKKNIEFNQGFGATETTSLGTSTFKG 436
K +H V N A+ A+ DAI KSK++ E Q A TT GT K
Sbjct: 677 KEKTANVQHSPEVLNDRSLASLSASRHADAISSKSKQHTEIAQ--PAFNTT--GTVLMKT 732
Query: 437 STNVDYSACGMP 472
+N+ SA P
Sbjct: 733 KSNLSDSALSTP 744
>SPAC2G11.13 |atg22||autophagy associated protein Atg22
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 529
Score = 26.6 bits (56), Expect = 3.0
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +2
Query: 20 IRFILKFYYKKGLSNQDIVPCILPFIHIYGLVISLMGHLSKGCKLVTMSKFSANLFF 190
I ++ +++ L I+ +LP +YGL+ + G + G + + F A LFF
Sbjct: 383 IPYLTEYFQLNSLQVVMIISILLPMAPLYGLLGYIPGFENAGIR-TSADVFRATLFF 438
>SPACUNK4.11c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 188
Score = 25.8 bits (54), Expect = 5.3
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +2
Query: 272 QAEHFRSVRNIICGAAPLAASDVDAILEKSKKNIEFNQGFG 394
QAE S +NI+ A VD + +K +N+E+ G+G
Sbjct: 21 QAEEELS-KNIVTDEHWSLAGKVDFLPQKMTRNVEYESGYG 60
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 25.4 bits (53), Expect = 7.0
Identities = 20/82 (24%), Positives = 39/82 (47%), Gaps = 8/82 (9%)
Frame = +2
Query: 179 NLFFDVL-KNQDVSLL-------YIVPPVAILLGKHPDVQAEHFRSVRNIICGAAPLAAS 334
+L D+L +N+D +L+ ++VPP+ L P ++H + + N + +
Sbjct: 701 SLLADLLSENKDANLMLIQEGKNHVVPPLFAKLLSSPKRSSKHLQEILNSSLDPSSGVVN 760
Query: 335 DVDAILEKSKKNIEFNQGFGAT 400
D+D L+ KK+ + G T
Sbjct: 761 DMDKALKFLKKSKAVVKELGLT 782
>SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 525
Score = 25.4 bits (53), Expect = 7.0
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = -1
Query: 561 GLFMYISPCSPIGIGSPVTGSANFSLKLT 475
G++M+I CS +GSP+ + KLT
Sbjct: 207 GMYMFIFMCSVASVGSPMGTGVAENPKLT 235
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 7.0
Identities = 13/37 (35%), Positives = 18/37 (48%), Gaps = 2/37 (5%)
Frame = +1
Query: 139 QRLQIGHNVKVFGESLFRCTEEPR--C*FTIHSASCS 243
Q I HN VFG+ + T +P C FT + C+
Sbjct: 289 QHNAIAHNPSVFGKPIGDLTSDPTNLCKFTFPTPECA 325
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,718,260
Number of Sequences: 5004
Number of extensions: 57461
Number of successful extensions: 164
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 158
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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