BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte8e13
(597 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 39 6e-04
SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2 |Sc... 29 0.68
SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 28 0.90
SPBC4.03c |||COPII-coated vesicle component Sfb3 |Schizosaccharo... 27 1.6
SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.6
SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyc... 27 2.1
SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase Cka1|Sch... 27 2.7
SPAC11D3.09 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Manual 27 2.7
SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 27 2.7
SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces pomb... 27 2.7
SPBC409.08 |||spermine family transporter |Schizosaccharomyces p... 26 3.6
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 25 6.3
SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces po... 25 6.3
SPCC645.13 |||transcription elongation regulator|Schizosaccharom... 25 6.3
SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 25 8.4
SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.4
>SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 38.7 bits (86), Expect = 6e-04
Identities = 21/63 (33%), Positives = 31/63 (49%)
Frame = +1
Query: 406 MSVQIISYGATITSIQXXXXXXXXXXXXAGFDTLEEYFQPRNPYFGATIGRYANIIRDAT 585
+ V I +YGA + +++ GF+ Y NP+FGATIGR+AN I +
Sbjct: 378 LEVSIANYGALVQAVRYKGRNLVN-----GFNDFSRYKLKENPFFGATIGRFANRIANGQ 432
Query: 586 MVV 594
V
Sbjct: 433 FEV 435
>SPBC21.05c |ral2||Ras guanyl-nucleotide exchange factor Ral2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 611
Score = 28.7 bits (61), Expect = 0.68
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = -1
Query: 594 HHHCGIPNDVCVAPNSSSEIWVSWLEIFF*SVESCDHIIRNAFFVRN 454
+HHCGI CV N SE ++ + +S +H I + FF N
Sbjct: 209 NHHCGIYRSNCVVINKDSE-FLQMCRPINTTQDSNEHSIGSLFFYLN 254
>SPAC926.04c |hsp90|swo1|heat shock protein
Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
Length = 704
Score = 28.3 bits (60), Expect = 0.90
Identities = 23/90 (25%), Positives = 38/90 (42%), Gaps = 4/90 (4%)
Frame = +1
Query: 106 FPVKMAEEGDEKASGDVEEDTKTVTPQPEPKIPDVELIVDGFGLMPKSMKVPKNRTSNAF 285
+P+++ + + EE+T+ V + + K P +E + D K K K T+
Sbjct: 204 YPIQLVVTREVEKEVPEEEETEEVKNEEDDKAPKIEEVDDESEKKEKKTKKVKETTTETE 263
Query: 286 EESKQ----MLKEIKVTNEEAKSPCSSSTN 363
E +K +VT EE S S TN
Sbjct: 264 ELNKTKPIWTRNPSEVTKEEYASFYKSLTN 293
>SPBC4.03c |||COPII-coated vesicle component Sfb3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 27.5 bits (58), Expect = 1.6
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = +1
Query: 208 VELIVDGFGLMPKSMKVPKNRTSNAFEESKQMLKE 312
+E ++D F + + +K+P+ NA E K +LKE
Sbjct: 384 IERLLDSFPAIFQDVKIPEPVVGNALEVVKLLLKE 418
>SPAC1687.09 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1379
Score = 27.5 bits (58), Expect = 1.6
Identities = 16/59 (27%), Positives = 25/59 (42%)
Frame = +1
Query: 178 TPQPEPKIPDVELIVDGFGLMPKSMKVPKNRTSNAFEESKQMLKEIKVTNEEAKSPCSS 354
TP PEP ++E + +G + KN S + S +L K+ N +K S
Sbjct: 245 TPTPEPSDKNLEKLKNGASKQASESQSLKNMESLSLARSSPILTSEKLKNGASKQAIES 303
>SPCC830.07c |psi1|psi|DNAJ domain protein Psi1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 379
Score = 27.1 bits (57), Expect = 2.1
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +1
Query: 112 VKMAEEGDEKASGDVEEDTKTVTPQPEP 195
+K A EGDEK G V++ + +P P
Sbjct: 260 IKFAGEGDEKPDGTVQDIQFVLAEKPHP 287
>SPAC23C11.11 |cka1|orb5|serine/threonine protein kinase
Cka1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 26.6 bits (56), Expect = 2.7
Identities = 11/15 (73%), Positives = 12/15 (80%)
Frame = -3
Query: 499 RILRPHHQERLFCQE 455
R+LR HQERL CQE
Sbjct: 307 RLLRYDHQERLTCQE 321
>SPAC11D3.09 |||agmatinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 394
Score = 26.6 bits (56), Expect = 2.7
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -2
Query: 293 DSSKAFEVRFLGTFIDLGINPNPSTINSTSGI 198
D S++F++ FLG D G + P SGI
Sbjct: 69 DKSQSFDIAFLGAPFDTGTSYRPGARFGPSGI 100
>SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 669
Score = 26.6 bits (56), Expect = 2.7
Identities = 9/22 (40%), Positives = 15/22 (68%)
Frame = -1
Query: 429 IRNYLNTHPIFRSPSVPSNHFN 364
IRN++ THP ++ SV ++ N
Sbjct: 624 IRNFITTHPSYKQDSVVNDEIN 645
>SPBC646.10c |||U3 snoRNP protein Nop56 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 497
Score = 26.6 bits (56), Expect = 2.7
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 127 EGDEKASGDVEEDTKTVTPQPEPK 198
+GDE+ DVEE +T++ +P K
Sbjct: 438 DGDEEVDIDVEETVETISEKPSKK 461
>SPBC409.08 |||spermine family transporter |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 539
Score = 26.2 bits (55), Expect = 3.6
Identities = 18/54 (33%), Positives = 27/54 (50%)
Frame = +1
Query: 82 YISF*NSKFPVKMAEEGDEKASGDVEEDTKTVTPQPEPKIPDVELIVDGFGLMP 243
YI+ NS V A G +GD+E+ ++ + PE L+V GFG+ P
Sbjct: 102 YITIVNSLLVVCSAF-GSSVIAGDLEDVSRDLKVGPEVANLSCSLMVVGFGIGP 154
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 25.4 bits (53), Expect = 6.3
Identities = 11/45 (24%), Positives = 21/45 (46%)
Frame = +1
Query: 241 PKSMKVPKNRTSNAFEESKQMLKEIKVTNEEAKSPCSSSTNIEVV 375
P + PK+ T+N +S + + +V +E S TN ++
Sbjct: 347 PHGISSPKHSTNNLSSKSGKFHSDFRVVSENVLLQARSETNSPII 391
>SPAC12G12.07c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 412
Score = 25.4 bits (53), Expect = 6.3
Identities = 16/76 (21%), Positives = 33/76 (43%), Gaps = 5/76 (6%)
Frame = +1
Query: 127 EGDEKASGDVEEDTKTVTPQPEPKIPDVELIVDGFGLMPKSMKVPKNRTSNAFEESK--- 297
E EKA D+ + + V P+ E + VD F P + + + + E
Sbjct: 166 EKSEKAVADLNDSSTDVVPESEVSFQTISSRVDNFFAAPLPSEQAEELIEDDYAEQPEQA 225
Query: 298 --QMLKEIKVTNEEAK 339
Q +++ ++++EA+
Sbjct: 226 VGQPIEQQSISDDEAR 241
>SPCC645.13 |||transcription elongation
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 25.4 bits (53), Expect = 6.3
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1
Query: 493 GFDTLEEYFQPRNPYFGATIGRYANIIRDATMV 591
GFD L +YF RN Y + +N ++DA ++
Sbjct: 560 GFDILYDYFVKRNRY--GVLHSKSNSVKDAYII 590
>SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1513
Score = 25.0 bits (52), Expect = 8.4
Identities = 17/71 (23%), Positives = 29/71 (40%)
Frame = +1
Query: 154 VEEDTKTVTPQPEPKIPDVELIVDGFGLMPKSMKVPKNRTSNAFEESKQMLKEIKVTNEE 333
+ E T P+P + + V GL+P S P ++ + E + ++
Sbjct: 1251 IGESKSTRKPRPTKRQRQIMKQV-AEGLLPASAIAPPKSSNEKKSSNNVKAVEAETKSKS 1309
Query: 334 AKSPCSSSTNI 366
KSP + TNI
Sbjct: 1310 EKSPKKNGTNI 1320
>SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 628
Score = 25.0 bits (52), Expect = 8.4
Identities = 7/17 (41%), Positives = 11/17 (64%)
Frame = -1
Query: 564 CVAPNSSSEIWVSWLEI 514
C+ N +E W+SW+ I
Sbjct: 351 CIKHNQHNEKWISWIRI 367
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,396,677
Number of Sequences: 5004
Number of extensions: 44995
Number of successful extensions: 153
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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