Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte8c19
(664 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 38 0.008
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.056
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 33 0.13
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 33 0.13
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.17
At3g49540.1 68416.m05414 expressed protein 32 0.30
At4g15410.1 68417.m02355 UBX domain-containing protein low simil... 31 0.52
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.2
At2g19710.1 68415.m02303 expressed protein contains Pfam profi... 30 1.6
At4g14830.1 68417.m02280 expressed protein 29 2.8
At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 3.6
At1g76770.1 68414.m08934 heat shock protein-related contains sim... 29 3.6
At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl... 28 4.8
At1g22080.1 68414.m02761 hypothetical protein 28 4.8
At1g66880.1 68414.m07601 serine/threonine protein kinase family ... 28 6.4
At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta... 27 8.4
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 37.5 bits (83), Expect = 0.008
Identities = 20/48 (41%), Positives = 31/48 (64%)
Frame = +2
Query: 410 QFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 553
+F R++ LP+ E+V+++L + GVLTI + P+ VKG R V IA
Sbjct: 132 KFWRQFKLPDNVDMESVKAKLEN-GVLTINLTKLSPEKVKGPRVVNIA 178
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 34.7 bits (76), Expect = 0.056
Identities = 18/50 (36%), Positives = 31/50 (62%)
Frame = +2
Query: 404 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 553
S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+
Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 33.5 bits (73), Expect = 0.13
Identities = 18/50 (36%), Positives = 30/50 (60%)
Frame = +2
Query: 404 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 553
S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+
Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 33.5 bits (73), Expect = 0.13
Identities = 19/50 (38%), Positives = 32/50 (64%)
Frame = +2
Query: 404 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKVPIA 553
S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+
Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 33.1 bits (72), Expect = 0.17
Identities = 15/35 (42%), Positives = 23/35 (65%)
Frame = +2
Query: 404 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 508
S QF RR+ LPE + V++ + +GVLT+T P+
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 32.3 bits (70), Expect = 0.30
Identities = 22/68 (32%), Positives = 39/68 (57%), Gaps = 7/68 (10%)
Frame = +2
Query: 437 EGAAPETVESRLSSDGVLTITAPRKVPDAVKGERKV---PI-AQTGPVRKE---IKDQSX 595
E AA E E+++ + + + AP KV AV+ E+K P+ A+ PV+ E +K++S
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156
Query: 596 RSQREGKV 619
+ ++E V
Sbjct: 157 QEEKEAVV 164
>At4g15410.1 68417.m02355 UBX domain-containing protein low
similarity to XY40 protein [Rattus norvegicus]
GI:2547025; contains Pfam profile PF00789: UBX domain
Length = 421
Score = 31.5 bits (68), Expect = 0.52
Identities = 19/52 (36%), Positives = 23/52 (44%)
Frame = +3
Query: 474 HQTGYSPSLRRGRCLTPSRERERCPSHRPVPFARRSRIRVXEANEKEK*KGL 629
H Y+PS +PSR R PS R P+ RSR E E E G+
Sbjct: 75 HSPDYTPSETSP---SPSRSRSASPSSRAAPYGLRSRGGAGENKETENPSGI 123
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/79 (22%), Positives = 33/79 (41%)
Frame = +2
Query: 278 DKFQVNLDVQHFAPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 457
D ++D+ EEI V+ D ++ + F R++ LPE
Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94
Query: 458 VESRLSSDGVLTITAPRKV 514
+ + DGVLT+ P+++
Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112
>At2g19710.1 68415.m02303 expressed protein contains Pfam profile:
PF03398 eukaryotic protein of unknown function, DUF292
Length = 937
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +1
Query: 532 REKGAHRTDRSRSQGDQGSEXKKPTRRKSRKVYEEL 639
R + +HR DRS +Q + SE RR R YE++
Sbjct: 358 RNEPSHRRDRSNAQRESFSEDHVSPRRNVRMQYEDM 393
>At4g14830.1 68417.m02280 expressed protein
Length = 152
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +2
Query: 416 VRRYALPEGAAPETVESRLSSDGVLTITAPR 508
V R+ LPE PE V DG L +T P+
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139
>At2g28680.1 68415.m03486 cupin family protein similar to legumin
(11S-globulin) from Ginkgo biloba [GI:949869], 11S
globulin from Avena sativa [GI:472867]; contains a 11-S
plant seed storage protein signature (PS00305)
Length = 356
Score = 28.7 bits (61), Expect = 3.6
Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%)
Frame = +2
Query: 404 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAVKGERK--VPIAQTGPVRK 574
S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+
Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199
Query: 575 EIKD 586
+IKD
Sbjct: 200 DIKD 203
>At1g76770.1 68414.m08934 heat shock protein-related contains
similarity to 17.9 kDa heat-shock protein [Helianthus
annuus] gi|11990130|emb|CAB55634
Length = 244
Score = 28.7 bits (61), Expect = 3.6
Identities = 19/73 (26%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +2
Query: 407 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKVPDAVKGERKVPIAQTGPVRKEIK 583
++F + + +P+ + +++R + DG LT+T P+K VKG + I + +E+K
Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKK----VKGITGLKIEEEDE-EEEMK 147
Query: 584 DQSXRSQREGKVE 622
+ + E K E
Sbjct: 148 EPIVEEKTEEKTE 160
>At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl
methyltransferase family protein similar to
defense-related protein cjs1 [Brassica
carinata][GI:14009292][Mol Plant Pathol (2001)
2(3):159-169]
Length = 352
Score = 28.3 bits (60), Expect = 4.8
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +3
Query: 414 SSDVTRCLKARRLRLWNRDCHQTGYSPSLRR 506
+S + + + R LWNRD H TG++ +++
Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFNNKVKK 181
>At1g22080.1 68414.m02761 hypothetical protein
Length = 475
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = -2
Query: 204 LFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKYGSND 76
L + P A L+R + PK RRR R+ SSK + + D
Sbjct: 305 LMDEAPRANLKRKQEQQDEPKRSKKRRRQRVARSSSKLHSNLD 347
>At1g66880.1 68414.m07601 serine/threonine protein kinase family
protein contains protein kinase domain, Pfam:PF00069;
contains serine/threonine protein kinase domain,
INTERPRO:IPR002290
Length = 1296
Score = 27.9 bits (59), Expect = 6.4
Identities = 13/41 (31%), Positives = 18/41 (43%)
Frame = -2
Query: 210 YSLFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKY 88
Y S G A + +ARP CWS R +E S+ +
Sbjct: 1041 YEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSF 1081
>At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase,
putative similar to trehalose-6-phosphate phosphatase
(AtTPPA) GI:2944178; contains Pfam profile PF02358:
Trehalose-phosphatase
Length = 377
Score = 27.5 bits (58), Expect = 8.4
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +2
Query: 110 SRRPRRLLDQHFGLALTPDDLLSVAAGPLLNREYYRPWRHLAAAARDVGSSIKVDKD 280
S PR+ L + F + + P+D S A L + H+AA A++ ++ +D D
Sbjct: 59 SSPPRKRLVKDFNIEIAPEDDFSQRAWMLKYPSAITSFAHIAAQAKNKKIAVFLDYD 115
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,547,906
Number of Sequences: 28952
Number of extensions: 262724
Number of successful extensions: 762
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 745
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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