Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= bmte8a23
         (527 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_A1ZBT5 Cluster: Mediator of RNA polymerase II transcrip...    83   5e-15
UniRef50_A7T1N3 Cluster: Predicted protein; n=2; Nematostella ve...    45   0.001
UniRef50_Q96G25 Cluster: Mediator of RNA polymerase II transcrip...    40   0.027
UniRef50_Q4SRU6 Cluster: Chromosome undetermined SCAF14488, whol...    38   0.19 
UniRef50_A3DI66 Cluster: Transketolase-like protein; n=1; Clostr...    33   3.1  
UniRef50_A5AML7 Cluster: Putative uncharacterized protein; n=1; ...    33   5.4  

>UniRef50_A1ZBT5 Cluster: Mediator of RNA polymerase II
           transcription subunit 8; n=8; Endopterygota|Rep:
           Mediator of RNA polymerase II transcription subunit 8 -
           Drosophila melanogaster (Fruit fly)
          Length = 252

 Score = 82.6 bits (195), Expect = 5e-15
 Identities = 46/103 (44%), Positives = 57/103 (55%), Gaps = 5/103 (4%)
 Frame = +3

Query: 234 KHIAQFTKVVSHVWEIISKGREDWEGESMRSTGMQPTHNIADTHALVTAVGTGKGLR--- 404
           K + Q+ KVVSHV +++SK RE+WE ES   TG+Q T ++ADT  LV AVG GKGL+   
Sbjct: 138 KQVTQYNKVVSHVLDMVSKAREEWEIESSSRTGIQQTSSMADTQLLVAAVGMGKGLKLTN 197

Query: 405 --PGMANMVGQXXXXXXXXXXXXXQLGPAPSTLPKAPSAIKTN 527
             PG   MV                 G     L KAPSA+KTN
Sbjct: 198 YGPGPGMMVPPSIRAPSPMGGPAMSPGNVQQQLGKAPSAVKTN 240


>UniRef50_A7T1N3 Cluster: Predicted protein; n=2; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 264

 Score = 45.2 bits (102), Expect = 0.001
 Identities = 29/98 (29%), Positives = 42/98 (42%)
 Frame = +3

Query: 231 QKHIAQFTKVVSHVWEIISKGREDWEGESMRSTGMQPTHNIADTHALVTAVGTGKGLRPG 410
           QK I    ++++ + +II+  +EDWEGE   S     +    D  ALV AV  GKGL+P 
Sbjct: 149 QKQINHLNELLTTLTDIITSAKEDWEGE-QNSASRSTSSTNPDIGALVAAVTFGKGLKPN 207

Query: 411 MANMVGQXXXXXXXXXXXXXQLGPAPSTLPKAPSAIKT 524
                 +              +        K PS+IKT
Sbjct: 208 KTTNSSKTRTSTSSEQGSAQWMNNKADQPGKHPSSIKT 245


>UniRef50_Q96G25 Cluster: Mediator of RNA polymerase II
           transcription subunit 8; n=45; Euteleostomi|Rep:
           Mediator of RNA polymerase II transcription subunit 8 -
           Homo sapiens (Human)
          Length = 268

 Score = 40.3 bits (90), Expect = 0.027
 Identities = 28/70 (40%), Positives = 37/70 (52%), Gaps = 3/70 (4%)
 Frame = +3

Query: 231 QKHIAQFTKVVSHVWEIISKGREDWEGESMRSTGMQPTHNIADTHALVTAVGTGKGL--- 401
           QK I    K+ S++ E ISK   + E   +R    + T N  DT+ALV AV  GKGL   
Sbjct: 137 QKQIQSLNKMCSNLLEKISKEERESESGGLRPN--KQTFNPTDTNALVAAVAFGKGLSNW 194

Query: 402 RPGMANMVGQ 431
           RP  ++  GQ
Sbjct: 195 RPSGSSGPGQ 204


>UniRef50_Q4SRU6 Cluster: Chromosome undetermined SCAF14488, whole
           genome shotgun sequence; n=2; Tetraodontidae|Rep:
           Chromosome undetermined SCAF14488, whole genome shotgun
           sequence - Tetraodon nigroviridis (Green puffer)
          Length = 324

 Score = 37.5 bits (83), Expect = 0.19
 Identities = 22/57 (38%), Positives = 31/57 (54%)
 Frame = +3

Query: 231 QKHIAQFTKVVSHVWEIISKGREDWEGESMRSTGMQPTHNIADTHALVTAVGTGKGL 401
           QK I    KV S + E ++  RE+ + ES+     +   N ADT+ LV AV  G+GL
Sbjct: 195 QKQIQALNKVCSGLLEKLNNPREERDAESISLRQNKQYFNPADTNTLVGAVAFGRGL 251


>UniRef50_A3DI66 Cluster: Transketolase-like protein; n=1;
           Clostridium thermocellum ATCC 27405|Rep:
           Transketolase-like protein - Clostridium thermocellum
           (strain ATCC 27405 / DSM 1237)
          Length = 278

 Score = 33.5 bits (73), Expect = 3.1
 Identities = 23/66 (34%), Positives = 28/66 (42%), Gaps = 1/66 (1%)
 Frame = +3

Query: 222 FIFQK-HIAQFTKVVSHVWEIISKGREDWEGESMRSTGMQPTHNIADTHALVTAVGTGKG 398
           FI  K H A+   V+ H +  I K   D  G      G  PT  I    A   ++G G G
Sbjct: 66  FILSKGHAAEAYYVLLHEYGYIDKSDLDAFGSFQAKLGGHPTKKIKGVEANTGSLGHGLG 125

Query: 399 LRPGMA 416
           L  GMA
Sbjct: 126 LATGMA 131


>UniRef50_A5AML7 Cluster: Putative uncharacterized protein; n=1;
           Vitis vinifera|Rep: Putative uncharacterized protein -
           Vitis vinifera (Grape)
          Length = 755

 Score = 32.7 bits (71), Expect = 5.4
 Identities = 14/44 (31%), Positives = 28/44 (63%)
 Frame = +3

Query: 219 SFIFQKHIAQFTKVVSHVWEIISKGREDWEGESMRSTGMQPTHN 350
           SF+ Q + A F+  V+ +  ++   R +W G+S++ +G+Q TH+
Sbjct: 549 SFLLQAYTANFSMGVAGLPWVVMSERGEW-GQSLQGSGLQETHS 591


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 466,039,794
Number of Sequences: 1657284
Number of extensions: 8433493
Number of successful extensions: 18534
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 18128
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18530
length of database: 575,637,011
effective HSP length: 95
effective length of database: 418,195,031
effective search space used: 33455602480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)