BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte5k03
(766 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP4H10.16c |||phosphatase activator |Schizosaccharomyces pombe... 28 1.7
SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein homolog|Schizosacch... 27 2.2
SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 27 3.9
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 26 5.1
SPBC1778.10c |ppk21|SPBC4C3.11|serine/threonine protein kinase P... 26 6.8
SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 26 6.8
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 26 6.8
SPAC1782.09c |clp1|flp1|Cdc14-related protein phosphatase Clp1/F... 26 6.8
SPBP4H10.03 |oxa102|oxa1, oxa1-2, oxa1sp2|mitochondrial inner me... 25 9.0
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 9.0
>SPBP4H10.16c |||phosphatase activator |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 295
Score = 27.9 bits (59), Expect = 1.7
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 363 PTAQVQTHPRFHKLLVFQSKQARTC 289
P + + P +HKLL+F K AR C
Sbjct: 231 PVSDPEYFPVYHKLLLFWQKPARKC 255
>SPBC28E12.06c |lvs1|SPBC3H7.16|beige protein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2609
Score = 27.5 bits (58), Expect = 2.2
Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 2/71 (2%)
Frame = -3
Query: 644 FPNSTRSSTASIFGAHSPG--GVFRACIIEFLTYSFNIKLSMDPDCSVCCTDMISRTFVK 471
FP SS +I + P G F+ I +++TY + L+++ S T+ + +
Sbjct: 585 FPVPLVSSQIAIISSLGPSYSGCFQGPINDYMTYKASTALTLEIQDSKSNTEDLLHSIQA 644
Query: 470 IIQTLIAFLMC 438
++ IAF+ C
Sbjct: 645 FSRSPIAFMSC 655
>SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated
membrane proteins, ESCRT 0 complex|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 610
Score = 26.6 bits (56), Expect = 3.9
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +3
Query: 597 VSSKDAGSATAGAVWKSRVHNSRMTQQILWVKASDICV 710
+ SK A + KSR+ +S QI+ +K +D CV
Sbjct: 40 IRSKSVDPKFAMRILKSRIDHSNPNVQIMALKLTDTCV 77
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 26.2 bits (55), Expect = 5.1
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 1/44 (2%)
Frame = -2
Query: 369 VSPTAQVQTHPRFHKLLVFQ-SKQARTCVYFLFLVYLKVSRLPN 241
V PTA H F K+L + S ++R CV+ + + +K S + N
Sbjct: 661 VDPTALAPGH-HFGKVLAYDASNESRRCVFEIPVTVMKPSSISN 703
>SPBC1778.10c |ppk21|SPBC4C3.11|serine/threonine protein kinase
Ppk21|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 25.8 bits (54), Expect = 6.8
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 2/50 (4%)
Frame = +3
Query: 171 DFLFKPIIMVTGFGPFANHPVNASWE--AVKLLDKQEIENKHRCELVLIE 314
D++F II F SW+ A+K+LDK+ I +++ + V IE
Sbjct: 53 DYVFGDIIGDGSFSKVRRATDKKSWKEYAIKVLDKKYIVKENKVKYVNIE 102
>SPCC1020.08 |||wybutosine biosynthesis protein
Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 688
Score = 25.8 bits (54), Expect = 6.8
Identities = 11/40 (27%), Positives = 18/40 (45%)
Frame = +3
Query: 270 QEIENKHRCELVLIEIPVTYENVDEFVPALWETHTPKLMI 389
+E C L L+ P+ Y ++EFV L E ++
Sbjct: 413 EEASRVRHCALSLVGEPIFYPYINEFVSMLHEREISSFLV 452
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 25.8 bits (54), Expect = 6.8
Identities = 9/33 (27%), Positives = 17/33 (51%)
Frame = +2
Query: 608 RCWQCYCGCCLEIKSTQQQNDTTDPLGQSVRYL 706
+C YCG C ++ T +++ + + RYL
Sbjct: 211 KCGHVYCGSCAKVLKTSKRSQSKCLVNDCGRYL 243
>SPAC1782.09c |clp1|flp1|Cdc14-related protein phosphatase
Clp1/Flp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 537
Score = 25.8 bits (54), Expect = 6.8
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +2
Query: 677 DPLGQSVRYLCEYIYYTSLS 736
D LG+ + +L + +YYTSLS
Sbjct: 6 DGLGEMIEFLEDKLYYTSLS 25
>SPBP4H10.03 |oxa102|oxa1, oxa1-2, oxa1sp2|mitochondrial inner
membrane translocase Oxa102|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 409
Score = 25.4 bits (53), Expect = 9.0
Identities = 6/10 (60%), Positives = 8/10 (80%)
Frame = -2
Query: 612 HLWSSQPWWS 583
H+WS PWW+
Sbjct: 108 HIWSGLPWWA 117
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 25.4 bits (53), Expect = 9.0
Identities = 17/60 (28%), Positives = 27/60 (45%), Gaps = 1/60 (1%)
Frame = -3
Query: 665 SAVVYS*FP-NSTRSSTASIFGAHSPGGVFRACIIEFLTYSFNIKLSMDPDCSVCCTDMI 489
+AVV S P N+ + + SP G +R ++ S + + D S CTD+I
Sbjct: 90 AAVVNSFLPFNNPKDDYKTRITLRSPSGSYRGSVVCLFKRSKFLPEELPADKSQICTDII 149
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,162,030
Number of Sequences: 5004
Number of extensions: 66628
Number of successful extensions: 189
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 367316502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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