Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= bmte5i05
(744 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 38 0.002
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 35 0.011
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.099
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 29 0.92
SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase Cgs2|Schi... 27 2.8
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.5
SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr 3... 26 6.5
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 26 6.5
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 26 6.5
SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase Gut2|Schi... 25 8.6
SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolo... 25 8.6
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 37.9 bits (84), Expect = 0.002
Identities = 47/177 (26%), Positives = 67/177 (37%), Gaps = 10/177 (5%)
Frame = +2
Query: 167 THASSLLVKQSISCETPMGVRQNPWTPTKRRGPIAAEASSPGPAVV-SLP------SLIG 325
TH SS + Q I T +G T R +AS P PA S+P +++
Sbjct: 924 THQSSTGLTQEI---TQLGSNMRLPTKLTRPSNDGRKASGPRPAAPPSIPPPLPVSNILS 980
Query: 326 KP---PPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAKGRALGPAASLHGR 496
P PP+ P AP + P A +A+ P P + A P +
Sbjct: 981 SPTSEPPKDHPPSAP-LSKPVSTSPAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPST 1039
Query: 497 WPPPRVEPTPAPCDYEPNKAARAVLDHAPAFSIGLRVSLPGPGSKTPAPNTYSMPPV 667
PP + PT P + + A + PA S + S+P P P P +PPV
Sbjct: 1040 APPVPI-PTSTPPVPKSSSGAPSAPPPVPAPSSEIP-SIPAPSGAPPVPAPSGIPPV 1094
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 35.1 bits (77), Expect = 0.011
Identities = 17/52 (32%), Positives = 27/52 (51%)
Frame = +2
Query: 302 VSLPSLIGKPPPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAK 457
V L S+ PP + + P + +K+DPL +I +A P P + G T+K
Sbjct: 1935 VPLSSMPNAPPSVASNAKLPPASNNRKVDPLEDILQAMPPPTTRKARGKTSK 1986
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.9 bits (69), Expect = 0.099
Identities = 46/178 (25%), Positives = 64/178 (35%), Gaps = 14/178 (7%)
Frame = +2
Query: 236 PWTPTKRRGPIAAEASSPGPAVVSLPS--LIGKPPPESRKPRAPAFTFGQKLDPLANIAK 409
P P+ R S+P P + PS + PP +P AP ++P N
Sbjct: 234 PSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPV-----SMNPAINSTS 288
Query: 410 AGPGPASYNTEGMTAKGRALGPAASLHGRWPPPRVEPTPAPCDYEP--NKAARAVLDHAP 583
P P R A + + + PPP P+ P N ++ + L P
Sbjct: 289 KPPLPPP--------SSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPP 340
Query: 584 -------AFSIGLRV---SLPGPGSKTPAPNTYSMPPVLGEAKEGSKRAAPAFSITGR 727
A SI L S P P AP+T PP L ++ S AP +I GR
Sbjct: 341 PPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPGR 398
Score = 25.8 bits (54), Expect = 6.5
Identities = 18/56 (32%), Positives = 22/56 (39%), Gaps = 1/56 (1%)
Frame = +2
Query: 215 PMGVRQNPWTPTKRRGPIAAEASSPGPAVVSLPSLIGKPPP-ESRKPRAPAFTFGQ 379
PMG P P PIA + PA LP PPP + P AP + +
Sbjct: 440 PMGAPAAP--PLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAPAAPVASIAE 493
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 28.7 bits (61), Expect = 0.92
Identities = 38/168 (22%), Positives = 61/168 (36%), Gaps = 5/168 (2%)
Frame = +2
Query: 233 NPWTPTKRRGPIAAEASSPGPAVVSLPSLIGKPPPESRKPRAPAFTFGQKLDPLANIAKA 412
NP+TP + P+AA + + S P++ PP + P P ++P
Sbjct: 955 NPYTPVAPQSPVAAAS-----RISSSPNM----PPSN--PYTPIAVASSTVNPAHTYKPH 1003
Query: 413 GPGPASYNTEGMTAKGRALGPAASLH-GRWPPPRVE-PTPAPCDYEPNKAARAVLDHAPA 586
G + + L P AS + PP V P+P+ V P
Sbjct: 1004 GGSQIVPPPKQPANRVVPLPPTASQRASAYEPPTVSVPSPSALSPSVTPQLPPVSSRLPP 1063
Query: 587 FSIGLRVSLPGP---GSKTPAPNTYSMPPVLGEAKEGSKRAAPAFSIT 721
S R +P P + P+ + S PP+ A S A+ + +T
Sbjct: 1064 VS-ATRPQIPQPPPVSTALPSSSAVSRPPIATSAGRSSTAASTSAPLT 1110
>SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase
Cgs2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 346
Score = 27.1 bits (57), Expect = 2.8
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = +1
Query: 292 TSRSISPFPYR*TASRIEETKSSSFYFR 375
T+ SI PFP +S +E KSS+F FR
Sbjct: 182 TTLSILPFPVNHGSSFGQELKSSAFLFR 209
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 6.5
Identities = 31/120 (25%), Positives = 50/120 (41%), Gaps = 3/120 (2%)
Frame = +2
Query: 293 PAVVSLPSLIGKPPPESRKPRAPAFTFGQKLDPLANIAKAGPGPASYNTEGMTAKGRALG 472
P +PS+ +PP AP+ T A++ P PA + +A +
Sbjct: 1493 PVPSMIPSVAQQPPSSVAPATAPSSTLPPSQSSFAHV----PSPAPPAPQHPSAAALSSA 1548
Query: 473 PAASLHGRWPPPRVEPTPAPCDYEPNKAARAVLDHAPAFSIGLRVSLP---GPGSKTPAP 643
PA + P R P AP EP + +A+ + APA ++G S GP + + +P
Sbjct: 1549 PADNS----MPHRSSPY-AP--QEPVQKPQAINNIAPATNLGTSQSFSPRMGPVNNSGSP 1601
>SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr
3|||Manual
Length = 334
Score = 25.8 bits (54), Expect = 6.5
Identities = 18/79 (22%), Positives = 37/79 (46%)
Frame = +2
Query: 203 SCETPMGVRQNPWTPTKRRGPIAAEASSPGPAVVSLPSLIGKPPPESRKPRAPAFTFGQK 382
S E ++ NP + +K + + + GP ++S P +I P + + + +K
Sbjct: 241 SSEMDSNLQTNPSSISKGKKNNSNNTTQKGPNIISSPQVINASSPVRKAGKKKS----KK 296
Query: 383 LDPLANIAKAGPGPASYNT 439
P + ++ + P ASYN+
Sbjct: 297 ALPTSPLSSSSP-DASYNS 314
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 25.8 bits (54), Expect = 6.5
Identities = 9/23 (39%), Positives = 17/23 (73%)
Frame = +3
Query: 660 HRYLVKRKKGVKELHRLSVSQVV 728
+++L +KG +ELHR +SQ++
Sbjct: 216 YQFLKDMRKGFRELHRKDLSQLI 238
>SPAC23C4.19 |spt5||transcription elongation factor
Spt5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 990
Score = 25.8 bits (54), Expect = 6.5
Identities = 13/28 (46%), Positives = 16/28 (57%)
Frame = +2
Query: 581 PAFSIGLRVSLPGPGSKTPAPNTYSMPP 664
PA++ G R GSKTPA N+ S P
Sbjct: 828 PAWNTGSRTPAWNSGSKTPAWNSGSRTP 855
>SPCC1223.03c |gut2||glycerol-3-phosphate dehydrogenase
Gut2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 649
Score = 25.4 bits (53), Expect = 8.6
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -1
Query: 732 FPRPVILKAGAALLLPSFASPSTGGI 655
FPR V+ AG ++LP + P GI
Sbjct: 307 FPRMVVPSAGVHVVLPEYYCPPNIGI 332
>SPAC26F1.14c |aif1|SPAC29A4.01c|apoptosis-inducing factor homolog
Aif1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 575
Score = 25.4 bits (53), Expect = 8.6
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = -2
Query: 236 GSGERPSESHTKLI 195
GS E+P+ES+TKLI
Sbjct: 246 GSDEKPTESYTKLI 259
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,046,178
Number of Sequences: 5004
Number of extensions: 67346
Number of successful extensions: 244
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 353266144
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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